Table of Contents
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User Programs—Customized Analysis
5.1.1 Entering a Calibration Formula Directly
5.1.2 Creating a Calibration Table
User Programs—Customized Analysis
Page 40
4. After entering the Program Name, save the program by touching
. Once the rest of the data in the table above is entered, touch
Save Program
again, then touch
Save Program
User Programs.
1. Scroll to Calibration and touch
2. Touch and edit each of the coefficients to complete the formula.
The coefficients determine the complexity of the formula. For
example, a simple linear relationship would have coefficients of
zero for c and d.
The Calibration screen shows the formula that will be used to
calculate concentration. This formula is shown at the top of the
screen. "C" stands for the calculated concentration. The "A"
corresponds to the absorbance reading, and the lowercase
characters "a", "b", "c", and "d" correspond to the coefficients
in the formula.
The Curve Fit screen allows concentration values to be entered and
the corresponding absorbance values to be read. After selecting the curve type,
the instrument will calculate the coefficients of the formula, based on the points
in the table.
1. On the Edit User Program screen, highlight
touch
Edit
2. On the Calibration screen, touch
+
3. Touch
4. Enter the first Standard Concentration value. Touch
5. Insert a blank sample (not the first standard) and touch
6. When the first calibration standard is ready to read, insert it in the sample
compartment. Touch
on the calibration curve.
7. Touch the
Add Point
8. Enter the second calibration point concentration. Touch
9. Insert the second calibration standard. Touch
10. Continue this process until all the standards are read (up to a maximum of
16 points).
. The program is now in the list of
Done
.
Edit
.
to add a point.
to read the absorbance and capture the first point
Read
icon again.
and
Calibration
.
Curve Fit
.
OK
Zero
.
OK
to read the absorbance.
Read
5940018UserPrograms.fm
.
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