Brother PT-2500PC User Manual page 178

When Align in Relation to Print Area
is off (no appears), the selected
objects are aligned based on their
locations in the layout.
Left
Horizontal Center
Right
Top
Vertical Center
Bottom
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When Align in Relation to Print
Area is on ( appears in front of
the command), the selected objects
are aligned based on the print area.
Left
Horizontal Center
Right
Top
Vertical Center
Bottom