NanoEnTek EVE User Manual
  1. Manuals
  2. Brands
  3. NanoEnTek Manuals
  4. Laboratory Equipment
  5. EVE
  6. User manual

NanoEnTek EVE User Manual

Automatic cell counter
Hide thumbs Also See for EVE:
Table of Contents

Advertisement

Quick Links

EVE
™
Automatic cell counter
User
Manual

Advertisement

Table of Contents
loading

Related Manuals for NanoEnTek EVE

Summary of Contents for NanoEnTek EVE

  • Page 1 ™ Automatic cell counter User Manual...
  • Page 2 The information in this manual is described as accurately as possible. Firmware and software changes and updates may change without prior consent or notification. Copyright © 2013 by NanoEnTek Inc. All rights reserved. Published in Korea. Documentation : NESMU-EVE-001E Revision history : V.0.0 Date : MAR 2013 : V.0.5...

  • Page 3: Table Of Contents

    PC software 26-33 Installation 26-27 Open the data 28-29 Cell gating Counting Spread sheet Prepare report Cleaning and maintenance Troubleshooting 35-36 Error codes Warranty Safety precautions 39-41 Product specifications Ordering information Technical support , Automatic Cell Counter ©2013 NanoEnTek Inc.

  • Page 4: Product Contents

    Product contents EVE™ is shipped with the following components. Please check that all items listed below were shipped, receiving the instrument. If any items are missing or damaged, contact your local distributor or e-mail sales@nanoentek.com. Automatic cell counter Power cord with 4 adaptor...

  • Page 5: Product Overview

    Using the same amount of sample that you currently use with the hemocytometer, the EVE™ takes less than 20 seconds per sample for a typical cell count and is compatible with a wide variety of eukaryotic cells and provides information on cell size.
  • Page 6 Uses disposable cell counting slides that eliminate washing steps and cross contamination between samples. — Saves and print cell count data including images using the EVE™ software and EVE™ USB drive. — Presents comprehensive data with graphical reports and as a .CSV...

  • Page 7: Description

    Slide port The slide port is used to insert the EVE™ Cell counting slide containing the sample with trypan blue stain into the counter for analysis. USB port...
  • Page 8 Description Rear and Power inlet Connect the counter to an electrical outlet using the supplied power cord and the side view appropriate plug, based on the electrical outlet configuration in your country. Image adjustment The image adjustment (focus) knob is used (focus) knob to adjust the image quality to obtain better contrast between live (bright centers) and dead...

  • Page 9: Installation

    Powering the instrument with an unapproved power cord may damage the instrument. 4. When you are ready to use, start the EVE™ by pressing the Power button. 5. When the instrument is turned on, the Start Up screen is displayed.

  • Page 10: Operation

    Operation 1. Press Settings from the Start-Up screen to display Settings. Settings The settings menu allows you to set up the following: · Count mode to select Counting type and Auto focusing options. The Counting type is to operate an instrument for cell counting or beads counting. The Auto focusing is to operate an instrument for AF on mode or AF off mode.
  • Page 11 After modifying any parameters, Apply button press to make the changes. Default button To restore default parameters, press 3. Press Save protocol button to create a new protocol. , Automatic Cell Counter ©2013 NanoEnTek Inc.
  • Page 12 Operation 4. Type protocol name and user name in the appropriate Cell mode fields, and press Save button again. parameters 5. Once a protocol is saved, it is available for use at any time. Press Load protocol button. 6. The protocol appears in the protocol menu. Use the up and down arrows to find your saved protocol.

  • Page 13: Date And Time Setup

    Operation 1. Turn on the EVE™ by pressing the Power button. The Start Date and up screen is displayed after a few seconds. time setup 2. Press Settings and then press Date and Time. 3. The Date/Time Properties screen is displayed. To select the month and day, scroll to the appropriate month using the arrow keys and then press the day on the calendar.
  • Page 14 ∙ The EVE™ is supplied pre-calibrated. To recalibrate your instrument, see page 24. ∙ The EVE™ memory holds one set of data. Save your data to the USB drive after each reading. You may transfer the data to your PC, using the USB drive immediately as described in Transferring data to a PC (page 21).

  • Page 15: Cell Counting

    3. Mix well the 10 µL of your sample and the 0.4% trypan blue stain using a pipette. 4. Load 10 µL of the sample mixture on EVE™ Cell counting slide (side A) using a pipette. The two chambers of the slide are labeled “A” and “B” for easy tracking of your samples.
  • Page 16 Operation 5. Insert the EVE™ Cell counting slide, sample side (side A) first into Cell counting the slide port on the instrument, you will hear a soft click, if the slide is pushed in correctly. Each chamber is counted separately.
  • Page 17 Focus knob. You are able to unlock the knob later, to adjust the image. ☞ For information on how to check counting results, refer to the page 19. , Automatic Cell Counter ©2013 NanoEnTek Inc.
  • Page 18 Operation Optimize the image for analysis such that: Cell counting ∙ Live cells have bright centers and dark edges. ∙ Dead cells have a uniform blue color throughout the cell with no bright centers. <Correct Image> <Incorrect Image 2> <Incorrect Image 1> Live cells have dark centers and are Dead cells have bright, blue centers counted as the dead cells.
  • Page 19 Save button (see page 21). 11. To see more details on the data as well as graphical representation of the data, press the More data button. » Press the Close button to return to the main screen. , Automatic Cell Counter ©2013 NanoEnTek Inc.
  • Page 20 13. To count the cells in the other side of the slide (side B), remove the EVE™ slide after side A is counted by pushing in the slide slightly and then pulling the slide out. Turn the slide around and reinsert into the slide inlet and repeat the procedure.

  • Page 21: Transferring Data To A Computer

    To delete all data from the .CSV file and start with a blank file, press Start .CSV file button. 4. Transfer the EVE™ USB drive to the USB port on your PC. You may open the .CSV file using a spreadsheet program. To see the image and generate a report, use the EVE™...

  • Page 22: Using The Beads Mode For Counting Beads

    (side A or B) using pipette. The two chambers of the slide are labeled “A” and “B” for easy tracking of your samples. 4. Insert the EVE™ Cell counting slide with beads into the slide port on the instrument, making sure that the sample side is inserted completely into the instrument.
  • Page 23 10. After counting beads, place instrument into Cell Count mode for counting cells by pressing Settings button and then pressing the Cells button. , Automatic Cell Counter ©2013 NanoEnTek Inc.

  • Page 24: Calibrating

    1. Press Settings and then press Calibration button. Calibrating 2. Check the current background level and press Yes button to recalibrate. 3. To recalibrate the EVE™, mix 10 µL trypan blue solution with 10 µL of a standard buffer, such as phosphate buffered saline (PBS). Mix thoroughly.
  • Page 25 4. Load 10 µL of the sample mixture to the chamber ports on Calibrating one side of the EVE™ Cell counting slide. Press Start. 5. After calibration is completed, press the OK button to restart the instrument and proceed to cell counting.

  • Page 26: Pc Software

    If you wish to archive data and generate reports, you must transfer data to your computer, and use the EVE™ software to generate and print reports (see instructions, below). Alternatively, data stored in the .CSV file may be transferred to your computer by the USB drive and imported into any spreadsheet program, without the need for EVE™...

  • Page 27: Installation

    5. Click ‘OK’ when Installation is complete. 6. When the software is installed, the PC software icon appears on your desktop. 7. Click on the PC software icon on your PC, Start-up screen will be shown. , Automatic Cell Counter ©2013 NanoEnTek Inc.

  • Page 28: Open The Data

    PC software 1. Insert the USB to USB port of computer. And Click Open Open the button to load data files transferred from the instrument. data 2. Select the file that need to adjust counting result in different condition. 3. Adjust the cell image by pressing the Zoom button. Navigate the fields by clicking the location you like to see on the grid.
  • Page 29 » Using each button, you can check individual result for viable cells, dead cells and total cells. 7. If modified image should be saved, then click Save changes button. , Automatic Cell Counter ©2013 NanoEnTek Inc.

  • Page 30: Cell Gating

    PC software 1. Select the saved file you wish to open and adjust range of Cell gating cell size using Cell gating button. 2. Determine the value for minimum cell size and maximum cell size using up and down arrow button. »...

  • Page 31: Counting

    Clumped cell Recognize individual cell <Result before recounting> <Result after recounting> » Before recounting, cells clumping together could be counted one cell. But after recounting, software algorithm will recognize and count each of them. , Automatic Cell Counter ©2013 NanoEnTek Inc.

  • Page 32: Spread Sheet

    PC software 1. Select the saved file you wish to open and click Spread Spread sheet sheet button. 2. User can check cell distribution according to cell size differences. And also, this information can be exported by Export button.

  • Page 33: Prepare Report

    3. Click Export to PDF button to obtain a printable version of the image and data. 4. Type the file name and select location for saving file. 5. You can check the file on the location you selected. , Automatic Cell Counter ©2013 NanoEnTek Inc.

  • Page 34: Cleaning And Maintenance

    Do not reuse the cell counting slides. The EVE™ does not need regular maintenance. To troubleshoot problems with EVE™, contact technical support (page 44). Do not perform any repairs or service on the EVE™ to avoid damaging the instrument.

  • Page 35: Troubleshooting

    ∙ Ensure that the sample covers the entire counting area and the EVE™ Cell counting slide is inserted completely into the counter. ∙ The EVE™ is designed to read samples from 1 × 10 cells/ mL to 1 × 10...
  • Page 36 ∙ Download a new version of the firmware from the website May be using a corrupted on a different USB drive and try updating the firmware firmware file or a damaged on the EVE™. Contact technical support (page 44) if the USB drive problem persists. Auto focusing ∙...

  • Page 37: Error Codes

    Error codes This section describes the error codes displayed by the EVE™ when it encounters a problem. Contact technical support (page 44) for details on error codes and if you need to send the instrument back for servicing. Position Reason...

  • Page 38: Warranty

    (1) year from date of purchase. If any defects occur in EVE™ during this warranty period, NanoEnTek will repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded: 1.

  • Page 39: Safety Precautions

    Class A equipment is intended for use in an industrial environment. In the documentation for the user, a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments, due to conducted as well as radiated disturbances. , Automatic Cell Counter ©2013 NanoEnTek Inc.
  • Page 40 Consignes de sécurité Examinez et suivez les consignes de sécurité ci-dessous : · N'installez pas l'instrument dans un endroit humide comme une serre ou un incubateur pour éviter un risque de choc électrique. Si de l'eau ou tout autre matériau pénètre dans l'instrument, l'adaptateur, ou l'entrées d'alimentation, débranchez le cordon d'alimentation et contactez un technicien de service.
  • Page 41 Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part I: General Requirements.” Instruments bearing the TUV symbol are certified by TUV Product Services to be in conformance with the applicable safety standards for the US and Canada. , Automatic Cell Counter ©2013 NanoEnTek Inc.

  • Page 42: Product Specifications

    5 - 60 µm Dimensions 27 cm (W) x 20 cm (D) x 19 cm (H) Weight 2.1 kg EVE™ Cell counting slide Material Polymethy methacrylate Dimensions 75 mm (L) x 25 mm (W) x 1.8 mm (H) Chamber depth 100 µm...

  • Page 43: Ordering Information

    Ordering information The following products can be used with the EVE™ and are available separately from NanoEnTek. Cat. No. Description Contents EVS-050 Cell counting slide 50 slides (100 counts, with 1 ea X 1.5 mL of trypan blue (0.4%)) EVS-1000...

  • Page 44: Technical Support

    Technical support Visit the our Website at www.nanoentek.com for : Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers. · For more information or technical assistance, please call or email. NanoEnTek Inc. (HQ)
  • Page 45 ™ Automatic cell counter NESMU-EVE-001E (V.1.5)

Table of Contents