Related Manuals for NanoEnTek ADAM MC2
Summary of Contents for NanoEnTek ADAM MC2
- Page 1 ADAM MC 2 A New Standard of Automated Cell Counter Instruction Manual Copyright NanoEnTek, Inc. All rights reserved.
- Page 3 Fax. +1-781-790-5649 The information in this manual is described as accurately as possible. Firmware and software changes and updates may change without prior consent or notification. Copyright © 2018 by NanoEnTek Inc. All rights reserved. Published in Korea. Documentation: NESMU-AMC2-001E...
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Page 5: Table Of Contents
Table of contents Introduction Setting General description Setting Technology Wifi Basic principle of counting Update Product Contents Power off ADAM-MC2 Lock AccuChip kit Power off Upon receiving the instrument Maintenance and cleaning Product Description Trouble shooting Front view of ADAM-MC2 Warranty Rear view of ADAM-MC2 Technical specifications... -
Page 6: General Description
Introduction General The ADAM-MC2, an automated cell counter, is a benchtop automated cell Description counter that performs cell count and viability measurements using AccuStain Solution. -
Page 7: Technology
To address these problems, NanoEnTek has developed the ADAM-MC2, which is based on a fluorescent microscopy technique for counting cells. The ADAM-MC2 utilizes sensitive fluorescence dye staining, LED optics and CMOS detection technologies to make the cell analysis more accurate and reliable. -
Page 8: Basic Principle Of Counting
Introduction Basic principle ADAM-MC2 is based on staining mammalian cell DNA with a fluorescent of counting dye, Propidium Iodide (PI). PI does not enter cells with intact membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. -
Page 9: Adam-Mc2
Product Contents Introduction ADAM-MC2 The contents of the ADAM-MC2 are listed below: Item Quantity Main device Instruction manual USB hub Wifi dongle Power cord Adapter AccuChip Kit Calibration Bead AccuChip kit The contents of the ADAM-MC2’s AccuChip Kit are listed below: Item AccuChip2x Kit AccuChip4x Kit... -
Page 10: Front View Of Adam-Mc2
Product Description Introduction Front view of The front view showing various parts of the ADAM-MC2 is shown below: ADAM-MC2 ① Door ② Power ③ ④ ⑤ ⑥ Control buttons Description ① Door Slide holder is inserted and ejected. ② Power on / off. Power ③... -
Page 11: Rear View Of Adam-Mc2
Introduction Product Description Rear view of The rear view showing various parts of the ADAM-MC2. ADAM-MC2 ① ② ③ USB Port PC Port Power plug Port Description ① USB Port Port for software update and save the data. ③ Power Plug Connects ADAM-MC2 power cord to wall outlet CAUTION ②... -
Page 12: Environmental Requirements
Initial Display 2. Press the power button for 2~3 seconds. If you get an error message, please contact your local distributor or sales@nanoentek.com. If booting is successful and no errors are detected, the home screens will be displayed as below. -
Page 13: Error Messages During Booting
[System State] during booting It appears when booting is not working properly. Turn off main power and restart device. If this message still appears after restarting, contact your local distributor or sales@nanoentek.com. Error code Cause 0x00000C00 Failure of X-axis sensor... -
Page 14: Count Setting
Getting Started Introduction Count setting Set the conditions in the ‘Setting’ tap before counting. [AccuChip] Set the AccuChip according to you are using. Accuchip Accuchip [Cell size] Set the minimum and maximum size of cell. Cell size [Dilution factor] When diluting sample, set the Dilution factor. CAUTION Factor values for the AccuStain Solution is already applied. -
Page 15: Introduction
General Operation Introduction Introduction Instruction are provided in this section for preparing the sample with AccuStain Solution for use with disposable AccuChip for automated cell count using the ADAM-MC2. Please check the procedure of sample preparation and testing below. For more detailed information, please refer to the next page. 1. -
Page 16: Sample Preparation
General Operation Introduction Sample Cultivate the required number of cells. preparation Add an appropriate volume of growth media or PBS to dilute to a final concentration of 5 x 10 cells/mL to 4 x 10 cells/mL. NOTE Concentration out of this range will result in errors. Refer to page 17 for more information about errors. - Page 17 General Operation Introduction Counting cell WARNING [Sample loading error] Be cautious of loading the correct volume of the sample into AccuChip. The instrument will not detect low or high sample volumes. Correct volume Low volume CAUTION Avoid bubbles which may negatively affect the result. ADAM-MC2, A New Standard of Automated Cell Counter...
- Page 18 General Operation Introduction Counting cell WARNING [AccuChip insert error] Completely insert AccuChip face up, in the direction of the arrow on the slide. The instrument will not detect if slides are inserted incorrectly. See pictures below for proper insertion. ( O ) ( X ) Correctly inserted Not fully inserted...
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Page 19: Run Sample
Measure Introduction Run Sample Start counting process by pressing ‘START’ . It may take about 2 minutes longer for auto focus at the initial test. While the test is in progress, you can check the cell images of each channel. ADAM-MC2, A New Standard of Automated Cell Counter... -
Page 20: Result Analysis
Measure Introduction Result Analysis The result will be displayed after being automatically calculated by ADAM- MC2 software. CLOSE * 1.10E6 = 1.10 X 10 cells/mL Number of Number of Title Viability Total cell Non-Viable cell HeLa_300μM H2O2 T1 (1.10E6) N1 (5.50E5) HeLa_100μM H2O2 T2 (2.20E6) N2 (5.50E5) -
Page 21: Result Analysis -Error Code
Measure Introduction Result Analysis - Error code Error code Cause Frames with errors are over 50% of total counting frame. Cells are more than 4X10 cells/mL. Cells are more than 2X10 cells/mL. Cells are less than 4X10 cells/mL. Cells are less than 5X10 cells/mL. Frame with error that contains cells whose diameter is larger than 100μm. -
Page 22: Data List
Data Introduction Data list ① ② ③ ④ ⑤ ⑥ ⑦ Control buttons Description ① Select all data in Data List. ② SEARCH Display the data of the selected date. ③ View and edit the data. EDIT Multiple data can be edited with the same settings. ④... -
Page 23: Edit
Data Introduction EDIT ① ③ ④ ② ⑤ CANCE L APPLY Control buttons Description ① Sample Edit the sample name. ② Cell size graph View the cell size graph each channel(T/N). ③ Cell size setting Set the min/max size of the cell. Set the dilution factor of sample. -
Page 24: Image
Data Introduction IMAGE ① ② ③ ④ ⑤ Control buttons Description ① Channel Select a channel. ② Original Check the original image. ③ Counted Check the counted cell image. ④ Frame Select a frame number of the channel. ⑤ Zoom-in/out Zoom in and out to check the cell image. -
Page 25: Mail
Data Introduction MAIL ① ② ③ Control buttons Description ① History Select an e-mail address from the list to send the Excel, PDF, and Image files of selected data. The e-mail address that received the files remains in the list. ②... -
Page 26: Setting
Setting Introduction Setting ① ④ ⑤ ② ③ ⑥ Control buttons Description ① Set the conditions in the setting tap before counting. Count setting Refer to page 10 for more information. ② Wifi Set the wifi to use the e-mail function. ③... -
Page 27: Wifi
• ADAM-MC2 can be updated only when the firmware or software file exists in the ‘AdamUpdate’ folder. The ‘ADAM MC2.eve’ file should be in the ‘AdamUpdate’ folder. • Do not rename the ‘AdamUpdate’ folder. The folder name should be ‘AdamUpdate’ . -
Page 28: Lock
Introduction Power off Lock Press LOCK before turning off the device. If there is no operation for 3 minutes, the lock function will be activated automatically. Whem the device is locked, the screen will be changed as shown below. Power off If you press the power button 2~3 seconds, then ‘Slide to shut down your PC’... -
Page 29: Maintenance And Cleaning
Maintenance and cleaning Introduction Maintenance 1. ADAM-MC2 does not need regular maintenance. 2. ADAM-MC2 has no replacement of consumable materials. and cleaning 3. Please clean the exposed surface of ADAM-MC2 frequently or before testing, using a soft cloth and isopropyl alcohol or deionized water. CAUTION Dispose of wipes in an appropriately labeled solvent contaminated waste container. -
Page 30: Trouble Shooting
Trouble shooting Introduction Trouble Problem Description Solution shooting ADAM-MC2 does • • No power from outlet Check power source. not power up • • Bad power cord. Replace. Inaccurate result • • Cell number may be out of Adjust the number of cells range. -
Page 31: Warranty
2. Repair or modification done by anyone other than NanoEnTek or an authorized agent. 3. Damage caused by substituting alternative parts. 4. Use of fittings or spare parts supplied by anyone other than NanoEnTek. 5. Damage caused by accident or misuse. 6. Damage caused by disaster. -
Page 32: Technical Specifications
Technical Specifications Introduction Technical ADAM-MC2 Specifications Measuring range 5x10 ~ 4x10 cells/mL Optimal range 5x10 ~ 2x10 cells/mL : For initial test, Analysis time < 25~50 sec/test max 2 min/test Voltage DC12V Current Objective lens 4W Green LED Camera CMOS camera Filter Excitation filter, Dichroic filter, Emission filter... -
Page 33: Product List
Product List Introduction Product List Cat. No. Product Contents Quantity 200 pcs AccuChip 2X AD2K-200 AccuChip2X Kit* 12.5 mL AccuStain Solution T 12.5 mL AccuStain Solution N 200 pcs AccuChip 4X 12.5 mL AccuStain Solution T AD4K-200 AccuChip4X Kit 12.5 mL AccuStain Solution N 12.5 mL AccuStain Solution T ADR-1000 AccuStain... -
Page 34: Safety Precautions
Safety Precautions Introduction Safety Review and follow the safety instructions below : Precautions Always ensure that the power supply input voltage matches the voltage • available at your location. To avoid the danger of electric shock, install the instrument per the •... -
Page 35: Safety Symbols
For more detailed information about disposal of your old appliance, please contact your city office, waste disposal service or visit our web-site, www.nanoentek.com. This product conforms to UL 61010-1, CAN/CSA C22.2 No.61010-1 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part l: Gen- eral Requirements.”... -
Page 36: Warnings
Warnings Introduction Warnings 1. After using device, please turn off main power. If not, it may cause malfunction or may reduce product life. 2. When turn off the device, be sure to lock the device with Lock button. If not, it may cause mechanical problem or error message when device is booting. -
Page 37: Technical Support
Technical Support Introduction Technical Visit the our Website at www.nanoentek.com for : Support Technical resources, including manuals, FAQs, etc. • Technical support contact information • Additional product information and special offers. • For more information or technical assistance, please call or email. - Page 40 MC 2 ADAM NESMU-AMC2-001E (V.0.0) NanoEnTek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel :+82-2-6220-7940 Fax:+82-2-6220-7999 NanoEnTek America, Inc. 240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA Tel: +1-781-472-2558 Fax: +1-781-790-5649 Email sales@nanoentek.com Website www.nanoentek.com...