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NanoEnTek EVE PLUS User Manual

Automated cell counter
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Automated cell counter
User Manual
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Summary of Contents for NanoEnTek EVE PLUS

  • Page 1 Automated cell counter User Manual Copyright NanoEnTek, Inc. All rights reserved.
  • Page 3 Fax. +1-781-790-5649 The information contained in this document is subject to change without notice. Firmware and software changes and updates may change without prior consent or notification. Copyright © 2019 by NanoEnTek Inc. All rights reserved. Published in Korea. Documentation: NESMU-EVE2-001E...

  • Page 5: Table Of Contents

    Table of contents Introduction Maintenance and cleaning Product contents Troubleshooting Product overview Warranty Features and benefits Product specifications Ordering information Product description Safety precautions Front view Safety symbols Side view Technical support Rear view Installation Install EVE PLUS Activation code Setting Setting menu Mode...

  • Page 6: Product Contents

    Please check that all items listed below were shipped, receiving the instru- ment. If any items are missing or damaged, contact your local distributor or e-mail sales@nanoentek.com. Automated cell counter Cell counting slides with 1.5 mL of Trypan blue (0.4%)

  • Page 7: Introduction

    Introduction Product EVE™ PLUS Automated cell counter uses state-of-the-art optics and image overview analysis for automatic cell counting. EVE™ PLUS is a benchtop counter de- signed to measure cell count and viability (live, dead, and total cells) accu- rately and precisely, using the standard trypan blue technique. Using the same amount of sample that you currently use with the hemo- cytometer, EVE™...

  • Page 8: Features And Benefits

    Introduction Features and User-friendly, compact design for simple, fast, automated cell count and • benefits viability measurements within 1 second. It provides data on cell size and is compatible with various types of eu- • karyotic cells, regardless of whether the cell size is small or large. Measures cell concentrations ranging from 1 ×...

  • Page 9: Product Description

    Product description Front view Item Description ① LCD touch screen Located in the front of the instrument contains buttons for all the functions needed and displays data from the cell count. ② Slide port The slide port is used to insert the EVE™ Cell counting slide containing the sample with trypan blue stain into the counter for analysis.

  • Page 10: Rear View

    Product description Rear view The front view showing various parts of the ADAM-MC2 is shown below: Control buttons Description ① USB port The USB port allows you to transfer and save the cell count data and image to your computer for record keeping and printing purposes.

  • Page 11: Installation

    Installation Install 1. After unpacking the instrument, place the instrument on a flat and dry sur- face. EVE™ PLUS 2. Connect adaptor and power cord, then plug the power cord to EVE PLUS. 3. Plug the power cord into the electrical outlet. Be sure to use only the power cord supplied with your instrument.

  • Page 12: Activation Code

    Installation Activation To use the EVE™ PLUS for the first time, you need the activation code, which is in the EVE™ slide box enclosed with the instrument. Entering the pro- code vided code into the pop up window allows normal cell counting. This code has a limit on the number of uses, and it is possible to use the counting function of 200 times per code.

  • Page 13: Setting

    Setting Setting menu Press SETTING from the startup screen to display settings. The setting menu allows you to set up the following: MODE to operate the instrument for cell counting (choose single cell) or • clumy cell counting (choose CLUMP CELL) PARAMETER (see below and next page for details) •...
  • Page 14 Setting Parameter 2. The parameters are described below: [Sensitivity] Sensitivity (refers to the contrast of the objects against the background). Adjusting the sensitivity higher makes instrument more sensitive to ob- jects; useful for cells that do not stain well with trypan blue while adjust- ing the sensitivity lower makes the instrument less sensitive and is useful if there is a lot of background.
  • Page 15 Setting Parameter 4. Type protocol name and user name in the appropriate fields, and press SAVE button again. 5. Once a protocol is saved, it is available for use at any time. Press LOAD button. 6. The protocol appears in the protocol menu. Use the up and down arrows to find your saved protocol.

  • Page 16: Calibration

    Setting Calibration 1. Press SETTING and then press CALIBRATION button. 2. Check the current background level and press YES button to recalibrate. 3. To recalibrate the EVE™ PLUS, mix 10 µL trypan blue solution with 10 µL of a standard buffer, such as phosphate buffered saline (PBS). Mix thor- oughly.
  • Page 17 Setting Calibration 4. Load 10 µL of the sample mixture to the slide ports on the EVE™ cell count- ing slide. Press START button. 5. After calibration is completed, press the OK button to restart the instru- ment and proceed to cell counting. There is no need to recalibrate each time the instrument is turned on.

  • Page 18: Date

    Setting Date 1. Press SETTING button and then press DATE button. 2. The Date/Time properties screen is displayed. To select the year, month and day, using the plus and minus button select the number. 3. Press APPLY button to make the Date/Time changes. 4.

  • Page 19: Operation

    Operation Recommend To obtain the best results, follow these recommendations: actions 1. Wear Protective gloves during sample handling. 2. Do not touch the optical surfaces of the EVE™ Cell counting slides. Hold the cell counting slides by the edges. 3. Use the EVE™ PLUS at room temperature only (5 - 40 °C). 4.

  • Page 20: Cell Counting (Auto Focus)

    Operation Cell counting 1. Press the power button to start the instrument. The Start-up screen is displayed. (Auto focus) 2. EVE™ PLUS is preset to CLUMP CELL mode for counting. If necessary to count single cell, select SINGLE CELL mode on SETTING. 3.
  • Page 21 Operation Cell counting 5. Insert the EVE™ Cell counting slide, sample side (side A) first into the slide port on the instrument, you will hear a soft click, if the slide is pushed in (Auto focus) correctly. Each slide is counted separately. 6.
  • Page 22 Operation Cell counting 8. After 10 seconds to count each sample, and the cell count for live, dead, (Auto focus) and total cells as well as percentage viability is displayed on the screen. Re- cord the cell count, or insert a USB drive and press SAVE button (see page 9.

  • Page 23: Cell Counting (Manual Focus)

    Operation Cell counting 1. Pressing and hold 3 seconds the Power button to start the instrument. (Manual focus) The Start-up screen is displayed. 2. Mix well the 10 µL of your sample and the 0.4% trypan blue stain using a pipette. 3.
  • Page 24 Operation Cell counting 5. Before cell counting press the MANUAL FOCUS button. (Manual focus) 6. For the correct focusing by pressing the ZOOM button. Select by press- ing the location you like to see on the navigator. While viewing cells in the zoom mode, use the focus knob to adjust the image.
  • Page 25 Operation Cell counting Optimize the image for analysis such that: (Manual focus) <Correct Image> Live cells have bright centers and dark edges. Dead cells have a uniform blue color throughout the cell with no bright centers. <Incorrect Image 1> Live cells have dark centers and are counted as the dead cells. <Incorrect Image 2>...
  • Page 26 Operation Cell counting 7. When you are satisfied with the image, press COUNT but- (Manual focus) ton. 8. With in 1 second to count each sample, and the cell count for live, dead, and total cells as well as percentage viability is displayed on the screen. Re- cord the cell count, or insert a USB drive and press SAVE button (see page 27).
  • Page 27 Operation Cell counting 10. The CALCULATOR button allows you to quickly calculate adjustments to (Manual focus) the cell suspension to obtain a desired concentration. 11. To count the cells in the other side of the slide (side B), remove the EVE™ slide after side A is counted by pushing in the slide slightly and then pull- ing the slide out.

  • Page 28: Data

    DATA Check 1. Push the DATA button to checking data lists. the image 2. Data lists and properties screen is displayed. When cell counting is done, all the information is automatically stored in the DATA and up to 500 lists can be stored. 3.
  • Page 29 DATA Check 4. To see more details counting results on the screen, press the ZOOM but- the image ton. 5. Choose CIRCLE ON button to see detail counting result. Then, use the navigator can see detail count result and viability result with blue and red color circle.

  • Page 30: Edit The Histogram

    DATA Edit the 1. Choose result on the lists, then press HISTOGRAM button to check detail. histogram 2. Determine the value for minimum cell size and maximum cell size using up and down arrow button. » Minimum cell size is used to determine the low range of cell size to in- clude in the measurement.

  • Page 31: Data Export (Report & Image)

    DATA Data export 1. To archive your data or generate a printed report, insert USB drive into (Report the USB port. & image) 2. Save your data on the USB drive by pressing the SAVE button on the main screen and data menu. 2-1) SAVE button on the main screen.

  • Page 32: Data Export (Csv File)

    DATA DATA export 1. Save CSV file on the USB drive by pressing the SAVE DATA (CSV) button (CSV file) on the main screen and data menu. The numerical data is also automati- cally saved as a .CSV file that can be opened with any spreadsheet program. 2.

  • Page 33: Data Export (Mail)

    1. Push the MAIL button to transfer data. (Mail) 2. Enter the file name using the keypad buttons displayed on the save menu. sales@nanoentek.com * To send data by e-mail, it is necessary to connect Wi-Fi. It can be set on Wi-Fi option in the SETTING menu.

  • Page 34: Maintenance And Cleaning

    Maintenance and cleaning Maintenance Clean the surface of the EVE™ PLUS with a damp cloth. To clean the LCD screen, turn off the EVE™ PLUS, disconnect the power ca- and cleaning ble, and clean the LCD screen with a soft cloth lightly moistened with LCD cleansing detergent.

  • Page 35: Troubleshooting

    Troubleshooting Trouble Inaccurate cell count shooting Problem Solution Sample handling • Do not insert the EVE™ Cell counting slide upside-down as this may introduce liquid into the instrument that could damage it. • Do not reuse the EVE™ Cell counting slides, as leftover dye from the previous reading may affect the next reading.
  • Page 36 Troubleshooting Trouble Saving and printing problems shooting Problem Solution Incorrect USB drive • Use the USB drive supplied with the counter or an USB 2.0 drive as some types of USB drive are not detected or recorded by the counter. •...

  • Page 37: Warranty

    2. Repair or modification done by anyone other than NanoEnTek or an authorized agent. 3. Damage caused by substituting alternative parts. 4. Use of fittings or spare parts supplied by anyone other than NanoEnTek. 5. Damage caused by accident or misuse. 6. Damage caused by disaster.

  • Page 38: Product Specifications

    Product specifications Product Environmental conditions specifications Operating power 100 - 240V~, 1.5A Frequency 50 / 60 Hz Electrical input 12 VDC, 1.0 A Installation site Indoor use only Operating temperature 5 - 40 °C Maximum relative humidity 20 - 80 % Altitude ≤...

  • Page 39: Ordering Information

    Ordering information Ordering The following products can be used with the EVE™ PLUS and are avail- able separately from NanoEnTek. information Cat. No. Description Contents 50 slides EVS-050 Cell counting slide 100 counts, with 1 ea X 1.5 mL of trypan blue (0.4%)

  • Page 40: Safety Precautions

    Safety precautions Safety Review and follow the safety instructions below: precautions Do not install the instrument in a humid place such as a greenhouse or an • incubator to avoid a danger of electric shock. If water or other material en- ters the instrument, the adaptor, or power inlet, disconnect the power cord and contact a service person.
  • Page 41 Safety precautions Consignes de Examinez et suivez les consignes de sécurité ci-dessous : sécurité N’installez pas l’instrument dans un endroit humide comme une serre • ou un incubateur pour éviter un risque de choc électrique. Si de l’eau ou tout autre matériau pénètre dans l’instrument, l’adaptateur, ou l’entrées d’alimentation, débranchez le cordon d’alimentation et contactez un tech- nicien de service.

  • Page 42: Safety Symbols

    Safety symbols Safety symbols The following symbols are found on the instrument and this document. Always use the equipment in the safest possible manner. Symbol Meaning Caution & Warning Protective earth (Ground) This instrument and consumables conforms to the Declaration of Conformity. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

  • Page 43: Technical Support

    Technical support Technical Visit the our Website at www.nanoentek.com for: support Technical resources, including manuals, FAQs, etc. • Technical support contact information • Additional product information and special offers. • For more information or technical assistance, please call or email.
  • Page 48 NESMU-EVE2-001E (V.0.0) NanoEnTek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel :+82-2-6220-7940 Fax:+82-2-6220-7999 NanoEnTek America, Inc. 240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA Tel: +1-781-472-2558 Fax: +1-781-790-5649 Email sales@nanoentek.com Website www.nanoentek.com...

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