Agilent Technologies Seahorse XFe Operating Manual
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Agilent Technologies Seahorse XFe Operating Manual

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Seahorse XFe Analyzer
Operating Manual

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Summary of Contents for Agilent Technologies Seahorse XFe

  • Page 1 Seahorse XFe Analyzer Operating Manual...

  • Page 2: Safety Notices

    FAR 12.211 (Technical Data) and 12.212 (Computer Software) and, for the Depart- ment of Defense, DFARS 252.227-7015 (Technical Data -Commercial Items) and DFARS 227.7202-3 (Rights in Commer- cial Computer Software or Computer Software Documentation). Seahorse XFe Analyzer Operating Manual...

  • Page 3: About This Manual

    About This Manual This manual contains information for operating and maintaining the Agilent Seahorse XFe Analyzer. “Introduction” Chapter 1 provides an introduction to the Agilent Seahorse XFe Analyzer. “Installation” Chapter 2 provides unpacking and installation instructions for the Agilent Seahorse XFe Analyzer.
  • Page 4 Seahorse XFe Analyzer Operating Manual...

  • Page 5: Table Of Contents

    Launch Wave Performing XF assays XFe status indicator Wave Controller widgets XFe Assays at Non-37 °C Temperatures Operational and assay guidelines for non-37 °C assays Set alarm (temperature tolerance range) Maintenance Cleaning and Routine Maintenance Seahorse XFe Analyzer Operating Manual...
  • Page 6 Troubleshooting Barcode errors Contact Information Worldwide technical support Ordering Additional Resources: Seahorse XFe Analyzer Operating Manual...

  • Page 7: Introduction

    Introduction General Information 8 Safety Considerations 9 Electrical hazards 9 Instrument Overview and Intended Use 10 Technical Specifications 11 This chapter provides an introduction to the Agilent Seahorse XFe Analyzer. Seahorse XFe Analyzer Operating Manual...

  • Page 8: General Information

    Introduction General Information General Information This manual covers several models of Agilent Seahorse XFe Analyzer: Instrument type Model numbers (32-bit) Model numbers (64-bit) Seahorse XFe96 101991-100, S7800A S7800B Seahorse XFe24 102238-100, S7801A S7801B Seahorse XFe Analyzer Operating Manual...

  • Page 9: Safety Considerations

    Always use a three-wire outlet with ground connection which is adequately rated for the load. The installation must comply with local safety regulations. Seahorse XFe Analyzer Operating Manual...

  • Page 10: Instrument Overview And Intended Use

    Instrument Overview and Intended Use Instrument Overview and Intended Use The Agilent Seahorse XFe analyzer measures the rate of change of dissolved oxygen and pH in the media immediately surrounding living cells cultured in a microplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells.

  • Page 11: Technical Specifications

    12 °C above ambient temp No gas or humidity control Software OS Windows 7, 32 bit Windows 10, 64 bit Data interface RS232c (internal) 64 bit barcode reader TCP/IP (external) USB Type B Seahorse XFe Analyzer Operating Manual...
  • Page 12 Introduction Technical Specifications Seahorse XFe Analyzer Operating Manual...

  • Page 13: Installation

    Installation Procedure 17 Suitable locations for the XFe system 17 Internal components of the XFe Analyzer 18 Setup and interconnects: cable installation 20 This chapter provides unpacking and installation instructions for the Agilent Seahorse XFe Analyzer. Seahorse XFe Analyzer Operating Manual...

  • Page 14: Unpacking And Component Identification

    These items must be removed prior to running the first assay. Agilent personnel will remove these shipping protection components from the XFe Analyzer During Installation. Instrument/Component Quantity Image XFe Instrument Seahorse XFe Analyzer Operating Manual...
  • Page 15 Installation Unpacking and Component Identification Instrument/Component Quantity Image XFe Controller 32 bit XFe Controller 64 bit Power cord (instrument) Power cord (controller) Power supply (controller) RS232 cable Seahorse XFe Analyzer Operating Manual...
  • Page 16 Installation Unpacking and Component Identification Instrument/Component Quantity Image USB cable USB extension cable Extra fan filters Seahorse XFe Analyzer Operating Manual...

  • Page 17: Installation Procedure

    The XFe should be positioned to allow accessibility to the power cord for easy disconnection. Avoid drafty areas, as well as areas experiencing significant vibration (such as C AU T I O N a centrifuge). Seahorse XFe Analyzer Operating Manual...

  • Page 18: Internal Components Of The Xfe Analyzer

    Probe head and injection manifold - The probe head consists of 24 or 96 “light pipes” to carry the optical signals to and from the sensors. The Injection manifold uses compressed air to inject compounds loaded into Sensor cartridge ports into the assay wells. Seahorse XFe Analyzer Operating Manual...
  • Page 19 Front stripper arm - Used for Sensor Cartridge removal at the end of assay runs. • Bar code reader - Reads bar code on sensor cartridge and cell plate. • Circulating fan - Helps to stabilize environment inside XFe Analyzer. Seahorse XFe Analyzer Operating Manual...

  • Page 20: Setup And Interconnects: Cable Installation

    3 A second cable (USB) connects the analyzer socket labeled "USB" to the USB port on the controller directly adjacent to the network (Ethernet) port. This port must be used for proper functioning of the instrument and barcode reader. (See Figure Seahorse XFe Analyzer Operating Manual...
  • Page 21 Two fans circulate air through the plenum containing the heater. A thermal fuse will disable the heater should it reach an abnormally high temperature. Seahorse XFe Analyzer Operating Manual...
  • Page 22 Installation Setup and interconnects: cable installation Seahorse XFe Analyzer Operating Manual...

  • Page 23: Basic Operation

    Wave Controller widgets 26 XFe Assays at Non-37 °C Temperatures 28 Operational and assay guidelines for non-37 °C assays 29 Set alarm (temperature tolerance range) 30 This chapter provides basic operating procedures for the Agilent Seahorse XFe Analyzer. Seahorse XFe Analyzer Operating Manual...

  • Page 24: Power And Warm Up

    (See Figure Figure 7. LCD message screen (ready) It is recommended that you allow at least 18 hours (overnight) for the NOTE instrument to fully warm and equilibrate to the set temperature. Seahorse XFe Analyzer Operating Manual...

  • Page 25: Performing Xf Assays

    To accept or cancel an assay if one or more wells did not calibrate properly after Calibration • Any errors that can occur during the run, such as barcode read errors for the Sensor Cartridge, Cell Plate, or a protocol error. Seahorse XFe Analyzer Operating Manual...

  • Page 26: Wave Controller Widgets

    1 Click the Tray widget to display the Tray widget dialog box (See Figure Figure 8. Tray widget dialog box 2 Click Tray Out, and remove the Utility Plate or Cell Plate. 3 To insert the tray and maintain the Target Temperature, click Tray In. Seahorse XFe Analyzer Operating Manual...
  • Page 27 Basic Operation Wave Controller widgets Probe control widget Use the Probes widget to load/unload a Sensor Cartridge or raise/lower probes. To display options and select the appropriate action, click the Probes widget. Figure 9. Probes widget Seahorse XFe Analyzer Operating Manual...

  • Page 28: Xfe Assays At Non-37 °C Temperatures

    XFe Assays at Non-37 °C Temperatures XFe Assays at Non-37 °C Temperatures Agilent Seahorse XFe Analyzers have been validated to deliver desired target temperatures in the range of 16-42 °C, provided the ambient room temperature is 12-20 °C below the target temperature, and in the validated operational room temperature range of 4-30 °C.

  • Page 29: Operational And Assay Guidelines For Non-37 °C Assays

    To adjust the Target Temperature (set point) using the up/down arrows, do the following: 1 Click the Temperature widget. (See Figure 11.) Figure 11. Temperature widget The Tray Heater dialog box is displayed. (See Figure 12.) Figure 12. Edit temperature settings (Tray Heater dialog box) Seahorse XFe Analyzer Operating Manual...

  • Page 30: Set Alarm (Temperature Tolerance Range)

    To ensure the Tray Temperature starts within the Tolerance Range, check the current temperature of the XFe Analyzer before beginning an assay. For any suspected temperature issues or unexpected temperature fluctuations, contact Technical Support. (See “Contact Information” on page 36.) Seahorse XFe Analyzer Operating Manual...

  • Page 31: Maintenance

    Cleaning and Routine Maintenance 32 Troubleshooting 33 Barcode errors 33 Contact Information 36 Worldwide technical support 36 Ordering 36 Additional Resources: 37 This chapter provides routine maintenance, troubleshooting, contact, and additional resource information for the Agilent Seahorse XFe Analyzer. Seahorse XFe Analyzer Operating Manual...

  • Page 32: Cleaning And Routine Maintenance

    If you encounter a spill of any reagents or liquids into the sample tray or C AU T I O N system, please contact Technical Support. Do not attempt to open the instrument unless specifically instructed to do so by an Agilent technical support representative. Seahorse XFe Analyzer Operating Manual...

  • Page 33: Troubleshooting

    Open Tray: Eject the Sensor Cartridge to inspect barcode quality or to reverse the Sensor Cartridge. • Manual: Manually input the Sensor Cartridge barcode information. Contact Agilent Seahorse Technical Support for this step. (See “Contact Information” on page 36.) • Cancel Assay: Cancel the assay. Seahorse XFe Analyzer Operating Manual...
  • Page 34 2 Call the appropriate regional Agilent Cell Analysis Technical Support telephone number on the Cartridge Barcode Manual Entry window for assistance with entering the Sensor Cartridge barcode info. (See Figure 14.) Figure 14. Cartridge Barcode Manual Entry window Seahorse XFe Analyzer Operating Manual...
  • Page 35 3 The Cell Plate barcode is located on the side of the plate. Write down the barcode information. 4 Click Close Tray. The Cartridge Barcode Manual Entry window appears. (See Figure 14 on page 34.) 5 Enter the Cell Plate barcode, and click Accept. Seahorse XFe Analyzer Operating Manual...

  • Page 36: Contact Information

    Ordering Link to online store: https://www.chem.agilent.com/store/ US Direct Ordering: • Email: css_afo_fax@agilent.com • Phone: 1.800.227.9770 option #1 #1 • Fax Purchase Orders to: 302.633.8901 Europe: Contact your local Customer Care Center https://www.agilent.com/en-us/contact-us/page Seahorse XFe Analyzer Operating Manual...

  • Page 37: Additional Resources

    Maintenance Additional Resources: Additional Resources: Wave User Guide: https://www.agilent.com/cs/library/usermanuals/public/Wave_2_ 6_User_Guide.pdf Software Download Page: https://www.agilent.com/en/support/cell-analysis/seahorse-xf-so ftware XFe Consumables Web Page: https://www.agilent.com/en/products/cell-analysis/seahorse-xfe- consumables Links to other useful https://www.agilent.com/en/promotions/cell-analysis-technology information: Seahorse XFe Analyzer Operating Manual...
  • Page 38 Maintenance Additional Resources: Seahorse XFe Analyzer Operating Manual...
  • Page 40 © Agilent Technologies, Inc. 2018 Second Edition, June 2018 *S7804-90001* S7804-90001_REV A...

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