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Step 5
After dispensing the injection solutions into the ports, with-
draw the tips from the ports carefully, holding the port loading
guide throughout the procedure. Remove the A/D port loading
guide and set aside for loading port D (if needed). Do not
tap any portion of the XF Sensor Cartridge in an attempt to
remove air bubbles from an injection port. This can cause
unintended leaking of injection solutions from the injection
ports.
NOTE: Forcefully dispensing injection solutions can over-
whelm surface tension at the injection port opening and result
in leaking of the solution from the injection port.
Step 6
Switch to the B/C port loading guide (red lettering). Orient
with the letter 'B' (green arrow, Figure 7) in the upper left-hand
corner, and place on top of the XF Sensor Cartridge. Repeat
loading procedure outlined in steps 3-5 for 'B', and injection
ports. If your experimental design requires an injection from
Port D, remove the B/C port loading guide and place the A/D
port loading guide on the XF Sensor Cartridge. Repeat loading
procedure outlined in steps 3-5 for injection port D.. Remove
and discard loading guide(s) once the entire XF sensor car-
tridge has been loaded.
B
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3
4
A
B
C
D
E
F
G
H
Figure 7 Orienting the B/C port loading guide.
www.agilent.com/chem/discoverxf
For Research Use Only.
Not for use in diagnostic procedures.
This information is subject to change without notice.
© Agilent Technologies, Inc. 2019
Printed in the USA, April 9, 2019
5994-0239EN r2
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Step 7
Visually inspect the injection ports for even loading (Figure
8). The solution should be in the port; make sure there are
no residual drops on top of the XF Sensor Cartridge. Once all
injection solutions have been loaded according to your ex-
perimental design, carefully transfer the XF Sensor Cartridge
(together with the XF Utility Plate) to the Seahorse XFe96/
XF96 Analyzer to start your assay.
IMPORTANT: Remove all port loading guides and lids for the
XF Sensor Cartridge and XF Utility Plate before placement
into the Analyzer.
12
Figure 8 Visual verification of injection port loading.
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