Summary of Contents for Agilent Technologies Seahorse XFe96
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Sensor Cartridge Injection Ports Basic Procedure A key feature of the Agilent Seahorse XFe96/XF96 Analyzer is the ability to inject solutions during the assay and observe changes in respiration or glycolytic rates in real time. This is accomplished by pipetting solutions into the Injection Ports on the XF Sensor Cartridge prior to beginning an XF assay.
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(green arrow) will be in the bottom left-hand corner tions prior to starting the assay, load the injection ports in (Figure 2). close proximity to the Seahorse XFe96/96 Analyzer. 5. A manual or automatic pipet may be used. Ensure any pipet is properly calibrated to accurately dispense volumes of 20.0 to 30.0 µL.
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Step 3 Step 4 Using a multi-channel pipette, first ensure tips are securely Place the A/D port loading guide (blue lettering) flat on top fitted onto the pipette before pipetting injection solutions. of the sensor cartridge. Orient the port loading guide so the Position the pipette tips (filled with your injection solutions) letter ‘A’...
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XF Sensor Cartridge remove air bubbles from an injection port. This can cause (together with the XF Utility Plate) to the Seahorse XFe96/ unintended leaking of injection solutions from the injection XF96 Analyzer to start your assay.