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ThermoFisher Scientific QuantStudio Series Manuals
Manuals and User Guides for ThermoFisher Scientific QuantStudio Series. We have
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ThermoFisher Scientific QuantStudio Series manual available for free PDF download: User Manual
ThermoFisher Scientific QuantStudio Series User Manual (66 pages)
Real-Time PCR System
Brand:
ThermoFisher Scientific
| Category:
Laboratory Equipment
| Size: 0 MB
Table of Contents
Table of Contents
3
CHAPTER 1 About the Software
7
Compatible Instruments
7
Compatible Data Files
7
CHAPTER 2 Workflow
9
CHAPTER 3 Set up a Plate File
10
Select a System Template or Existing Plate File to Set up a New Plate File
10
Confirm or Edit Run Method
12
Run Method Elements
12
Apply the Recommended Run Method for Your Master MIX
12
Edit Temperature Ramp Rate, Temperature, and Time Length for a Step
13
Add or Remove a Step
13
Add or Remove a Stage
13
Add, Edit, or Remove a Pause Cycle in a PCR Stage
14
Edit the Ramp Increment for the Melt Curve Dissociation Step
15
Enable, Edit, or Disable Auto Delta
15
Enable, Edit, or Disable Veriflex Zones
16
Confirm or Edit Filter Settings
16
Confirm or Edit Plate Setup
17
Select Plate Wells
17
Add Samples and Assign to Plate Wells
17
Add Targets or SNP Assays and Assign to Plate Wells
21
Edit Reagent Information
25
Select a Passive Reference
25
Set up the Standard Curve
26
Add Biogroups and Assign Samples
27
Edit Plate File or Data File Information
27
Review the Plate File and Save
28
Export a Plate Setup File
28
CHAPTER 4 Review and Analyze Data
29
Workflow: General Procedures to Review the Primary Analysis Results
29
Open a Data File
30
Review Results in the Amplification Plot
30
Evaluate the Overall Shape of the Curves in the Amplification Plot
30
Review the Amplification Status for each Well
31
Review or Edit Threshold Settings in the Amplification Plot
31
Review or Edit Baseline Settings in the Amplification Plot
33
Optimize Display of Negative Controls in the Amplification Plot
33
Identify and Omit Outliers from Analysis
34
Review Results in the Multicomponent Plot
35
Review Results in the Raw Data Plot
36
Review Results the Melt Curve Plot
36
Review QC Alerts in the Well Table
37
Edit Primary Analysis Settings
37
View or Edit C Settings
37
View or Edit QC Alerts Settings
38
View or Edit Melt Analysis Settings
39
Use the Analysis Settings from Another File
39
View Instrument Calibration Results
40
Review Roi/Uniformity Calibration Results
40
Review Background Calibration Results
40
Review Dye Calibration Results
41
Override the Calibration Data
41
Perform Additional Analysis
42
CHAPTER 5 Export Results
43
Export the Well Table
43
Export Plate Layout as an Excel ™ Spreadsheet
43
Export Plate Layout Image
43
Export Images of Plots
43
Export Data in the Quantstudio ™ File Format
44
Export Data in the RDML Format
44
Generate a Report
44
CHAPTER 6 General Procedures to Analyze Data in the Quality Check Tab
47
View the Post-Run Summary
47
Configure the Layout of the Quality Check Tab
47
Review Individual Well Results in the Well Table
48
Review Replicate Group Results
48
Configure General Plot Settings
48
CHAPTER 7 Manage Plate Files and Data Files
49
About System Templates and Plate Files
49
About Data Files
50
Save a Plate File or Data File
50
Restrict Editing of a Plate File or Data File
50
Add a Plate File to My Plate Files
51
Search for a Plate File or Data File
51
CHAPTER 8 Manage Analysis Modules
52
About Analysis Modules
52
Select an Analysis Module
53
CHAPTER 9 Manage Export Settings
54
About Export Settings
54
View or Edit Export Settings
54
View Files in the Export Settings Library
55
Download an Export Settings File
55
Import an Export Settings File
56
CHAPTER 10 About Data Analysis
57
About the Quantification Cycle (C Q )
57
About Melt Curve Analysis
57
Primary Analysis Settings Overview
58
Settings Overview
58
Guidelines for Manual Threshold and Baseline Settings for C Analysis
59
Melt Analysis Settings Overview
59
Plots Overview
59
Amplification Plot Overview
59
Raw Data Plot Overview
60
Multi-Component Plot Overview
60
Melt Curve Plot Overview
61
APPENDIX A Troubleshooting
62
Documentation and Support
63
Related Documentation
63
Customer and Technical Support
63
Limited Product Warranty
63
Index
64
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