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SILICYCLE MiniBlock manual available for free PDF download: User Manual
SILICYCLE MiniBlock User Manual (60 pages)
Brand:
SILICYCLE
| Category:
Laboratory Equipment
| Size: 17 MB
Table of Contents
Table of Contents
2
Usage and Safety Precautions
6
Cleaning
6
Maintenance
6
Storage
6
Temperature
6
Pressure
6
Components
6
Document Conventions
6
Figure 1: Silicycle Miniblock
7
General
7
Table 1: Minblock Physical Description
7
Figure 2: Reaction Tube Components
8
Figure 3: Silicycle Miniblock Components
8
Figure 4: Glass Reaction Tubewith Teflon® Heat Shrink Sleeve
8
The Silicycle Miniblock Reaction Tubes
8
Figure 5: Types of Pinch-Tube Inserts
9
Figure 6: Valve Mechanism in Closed Position
9
Figure 7: Silicycle Miniblock with Valve Key
9
Figure 8: Reaction Tube Configurations
10
Table 2: Silicycle Miniblock Configurations
10
Ambient Temperature Configurations
11
Controlled Temperature Configurations
11
Figure 10: Silicycle Miniblock Heat Transfer Blocks
11
Figure 9: Red Plugs Configurations
11
Plugs for Unused Pinch-Tube Inserts
11
Combining Red and Blue Silicycle Miniblocks into 96-Well Format
12
Silicycle Miniblock Configurations
10
Silicycle Miniblock System Materials
12
Silicycle Miniblock Metal Components
12
Reaction Tube Materials
12
Pinch-Tube Insert Materials
12
Figure 11: Collection Configuration 96-Positions
12
Septa Materials
13
Figure 12: Silicycle Miniblock with Heat Transfer Block
14
Silicycle Miniblock Temperature Control
14
Figure 13: Inert/Purge Manifold
15
Silicycle Miniblock Reactions Requiring an Inert Atmosphere
15
Silicycle Miniblock Assembly and Reaction Setup
16
Pre-Assembly Checks
16
Figure 14: Slide Valve Operation
16
Figure 15: Inspecting Pinch-Tubes
17
Figure 16: Pinch-Tube Extension
17
Silicycle Miniblock Assembly
18
Setup for Ambient Temperature Reaction
18
Setup for Controlled Temperature Reaction
18
Figure 17: Alignmentsplates Installed
18
Figure 18: Heat Transfer Block Installed
19
Figure 19: Insert Reaction Tubes
19
Inserting Reaction Tubes
19
Figure 20: Male / Female Luer Fitting
20
Figure 21: Seating Reaction Vessels
20
Sealing for Reactions Not Requiring Inert Conditions
20
Figure 22: Septum Layer and Cover Plate
21
Figure 23: Inert / Purge Manifold
22
Sealing for Reactions Requiring Inert Conditions
22
Closing the Silicycle Miniblock Valve
23
Figure 24: Silicycle Miniblock with Valve Key
23
Reaction Setup, Mixing and Incubation
24
Resin Addition - Solid-Phase Reactions
24
Resin Addition - Serial
24
Resin Addition - Parallel
24
IRORI® Kans
24
Resin Swelling
24
Inert Atmosphere
24
Figure 25: Dispensing Plate
24
Adding Solvents and Reagents
25
Reaction Mixing and Incubation
26
Shaking Stations
26
Securing Silicycle Miniblocks on the Shaking Station
26
Balancing the Shaking Station
26
Figure 26: Shaking Stations
26
Pre-Operational Shaking Station Check
27
Figure 27: Aligning Silicycle Miniblocks on the Shaker Top (with Optional Recirculator Manifold)
27
Figure 28: Securing Silicycle Miniblocks on the Shaker Top
27
Setting Agitation Speed
28
Incubation
28
Attaching the Manifold to the Shaker
28
Figure 29: Attaching the Recirculator Manifold to the Shaker Top
28
Connecting to the Recirculator
29
Testing the Recirculation System
29
Washing, Purification and Product Collection
30
Solvent Removal and Resin Washing
30
Removing Solvents to Waste on the Shaking Station
30
Accelerating Solvent Drainage
30
Figure 30: Slide Valve Operation
30
Air Push Assist Device
31
Positive Pressure Manifold
31
Figure 31: Air Push Assist Device
31
Figure 32: Positive Pressure Manifold
31
Inert Atmosphere Manifold
32
Washing
32
Purification and Clean-Up
32
Solid-Phase Extraction (SPE)
32
Figure 33: Transfer to Second Silicycle Miniblock Containing SPE Packed Tubes (Using Transfer Adapter)
32
Scavenger Resins
33
Product Collection / Cleavage
33
Figure 34: Examples of Silicycle Miniblock Collection Racks and Plates
33
Table 3: SPE Development Kits Available from Silicycle
33
Collection Rack Selection
34
Figure 35: Collecting Products
34
Figure 36: Vacuum Collection Base, Shown with a 24-Positions Rack
34
Table 4: Collection Plates, Racks and Vessels
36
Collection Vessel Positioning
37
Figure 37: Proper Height Alignment for Collection Tubes
37
Figure 38: Positioning Silicycle Miniblock Components for Collecting into 48 Test Tubes Rack
38
Figure 39: Pinch-Tube Extension
38
Maintenance
39
Cleaning Procedure
39
Post-Collection Rinse
39
Figure 40: Tube Removal Tool
39
Silicycle Miniblock Cleaning
40
Replacing Consumable Components
40
Replacement of Pinch-Tube Inserts
40
Figure 41: Remove Valve Insert Lock-Plate
41
Figure 42: Remove Pinch Tube Inserts
41
Figure 43: Compression Cords Replacement
42
Replacement of Compression Cords
42
Troubleshooting
43
Jammed Slide Valve
43
Leakage / Solvent Loss
43
Figure 44: Leakage Points
43
Preventive Maintenance
45
Figure 45: Exploded View of Silicycle Miniblock Reactor
46
Table 5: Silicycle Miniblock Spare Parts
47
Appendix A: Heat Shrinking Teflon® Sleeves Onto Glass Reaction Tubes
48
Apparatus
48
Procedure
48
Figure 46: Glass Reaction Tube with Teflon® Sleeve
48
Quality Checks
49
Incomplete Heat-Shrinking
49
Defective Heat-Shrinking
49
Appendix B: Resin Dispenser: Operating Instructions
50
Figure 47: Resin Dispensing Plate
50
Operation Description
50
Overview
50
Dispensing
51
Resin Dispenser Setup (Procedure Written for the 48-Positions Version)
51
Figure 48: Resin Dispenser (13742086)
51
Table 6: Standard Configurations
51
Table 7: High Volume Kit (Sold Separately)
51
Dispensing Procedure
52
Figure 49: Resin Dispenser Closed Position
52
Figure 50: Resin Dispensing Spreader
52
Dispensing to Microtiter / Deep Well Plates
53
Figure 51: Dispensing Powder
53
Figure 52: Replacing the Plate
54
Resin Cleaning
54
Resin Dispenser Calibration
54
Figure 53: the Vacuum Collection Base Set (13742008)
55
Table 8: Collection Rack Configurations
55
Assembly Diagrams
56
Figure 54 : 12 X 100 MM Test Tubes (8 Ml)
56
Figure 55 : 12 X 75 MM Test Tubes (6 Ml)
56
Figure 56 : Deep Well Plate (Various Volumes)
56
Figure 57 : Tall Minitube Rack (2.5 Ml)
56
Figure 58 : 16 X 100 MM Test Tubes (15 Ml)
57
Figure 59 : 15 X 45 MM Vials (3.7 Ml)
57
Figure 60: 17 X 61 MM Vials (7.5 Ml)
57
Figure 61 : 21 X 70 MM Vials (15 Ml)
57
Figure 62 : 28 X 95 MM Vials (40 Ml)
57
Appendix C: Silicycle Miniblock Collection Rack Configurations
55
Appendix D: Determining Correct Collection Tube Height
58
Instructions for the Go/No-Go Test
58
Figure 63: Tube Heights Are Correct. "GO
58
Figure 64: Tube Heights Are too Low. "NO-GO
58
Figure 65: Tube Heights Are too High. "NO-GO
58
Procurement
59
Appendix E: Chemistry Applications
59
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