Sartorius Biostat RM 20 Manuals

Manuals and User Guides for Sartorius Biostat RM 20. We have 1 Sartorius Biostat RM 20 manual available for free PDF download: Operating Instructions Manual

Sartorius Biostat RM 20 Operating Instructions Manual (97 pages)

Bioreactor prepared for Biobrain

Brand: Sartorius | Category: Laboratory Equipment | Size: 2 MB

Table of Contents
3
About this Document
7
Scope
7
Accompanying Documents
7
Target Groups
7
Symbols Used
8
Warnings
8
Other Symbols
8
Safety Instructions
9
Intended Use
9
Personnel Qualification
9
General Safety Instructions
9
Significance of These Instructions
10
Proper Working Order of the Device
10
Safety Equipment
10
Danger Due to Electrical Power
10
Unsuitable Power Supply Cables
11
Danger Due to Gasses
11
Danger of Fire Due to Pure Oxygen (O )
11
Danger Due to Carbon Dioxide
11
Accessories, Consumables, and Spare Parts
12
Emergency Instructions
12
Personal Protective Equipment
12
Danger Due to Leaked Substances
13
Danger of Scalding Due to Defective Components
13
Danger of Chemical Burns from Contact with Feed and Culture Media
13
Danger of Contamination from Contact with Feed and Culture Media
13
Danger Due to Components under Pressure
13
Danger of Injury from Escaping Substances
13
Risk of Death from Energy that Is Unexpectedly Released
14
Danger Due to Bursting of the Culture Bag
14
Hot Surfaces
14
Heating Mats
14
Moving Parts
15
Unexpected Startup of the Device
15
Heavy Weight of the Device
15
Device Description
16
Device Overview
16
Connections
18
Rear Connections
18
Load Cell Module Connections
19
Connections on the Left and Right of the Device
19
Load Cell Module (Optional)
20
Culture Bag Holder
20
Hood for Culture Bag Holder 20 | 50
20
Aeration Module (Optional)
21
Heating Mats
21
Temperature Sensor
22
Filter Heaters
22
Display
22
Safety Equipment
23
Main Switch
23
Overheating Protection
23
Symbols on the Device
23
Temperature Control
24
Setup
25
Acclimatization
25
Conditions at the Place of Installation
25
Unpacking the Device
26
Laboratory Energy Sources
26
Emergency Shut-Off Equipment
26
Power Supply
26
Gas Supply
27
Getting Started
29
Installation Kit
29
Installing the Culture Bag Holder
29
Culture Bag Holder with Heating Mats
29
Installing the Temperature Sensors
30
Temperature Sensor in Connection with Heating Mats
30
Connecting the Compressed Air and CO
31
Supply
31
Connecting to the Power Supply
31
Switching the Device on and off
32
Connecting the Device to Decentralized Control Systems (DCS)
33
Options for Connecting the Device to Other Devices
33
Options for Connecting to Deltav Systems
34
Changing the Communication Settings
36
Connecting the Device to Ethernet
37
Connecting the Device to the Biostat
37
RM Control Unit
37
Connecting the Device to Decentralized Control Systems
37
Connecting the Device to Profibus Networks
37
Operation
38
Process Sequence Overview
38
Operating the Control Elements
38
Main Screen" Menu
39
Overview" Menu
40
Aeration
40
Aeration with Air ("GASFLOW 1," "GASFLOW 2" Menu)
40
Aeration with CO ("CO " Menu)
41
Rocker Speed, Rocker Angle | "SPEED" and "ANGLE" Menus
42
Temperature | "TEMP 1" and "TEMP 2" Menus
43
Tray Position | "POSITION" Menu
43
Weighing Function | "LOADCELL" Menu
44
Loadcell Zero" Submenu
45
Loadcell Tare" Submenu
45
User Management | "User Level" Menu
46
Alarms | "Alarm" Menu
48
Settings | "Settings" Menu
48
Setting the Time and Date
48
Displaying Service Information
49
Setting the Culture Bag Size
50
Selecting the Control Mode
51
Setting the Controller Behavior after a Restart
53
Starting the Timer for the Process Sequence
53
Preparing the Culture Bag
54
Installing Culture Bags on the Culture Bag Holder
54
Installing the Filter Heaters
54
Connecting the Air Supply
55
Inflating the Culture Bag
56
Filling the Culture Bag with the Culture Medium
56
Setting the Cultivation Parameters and Starting Cultivation
57
Sampling and Inoculation
58
Preparing Sampling or Inoculation
58
Inoculation
59
Sampling
60
Completing the Process, Cell Harvesting, and Shutting down the Device
60
Stopping the Rocker Movement
60
Emptying the Culture Bag and Cell Harvesting
61
Switching off the Device
61
Cleaning and Maintenance
62
Preliminary Steps
63
Cleaning
63
Maintenance
64
Device Servicing by Operating Personnel
64
Servicing the Device by Authorized Personnel
64
Faults
65
Troubleshooting
65
Hardware-Related Faults
66
Contamination Troubleshooting Table
66
Temperature Control" Troubleshooting Table
67
Aeration and Ventilation" Troubleshooting Table
68
Alarm Messages
69
Decommissioning
72
Decommissioning the Device
72
Transport
72
Transporting the Device
72
Storage and Shipping
73
Storage
73
Returning the Device and Parts
73
Disposal
74
Information on Decontamination
74
Disposing of Device and Parts
74
Information on Disposal
74
Technical Data
75
Dimensions
75
Weights, Materials
75
Device Components
76
Heating Mat
76
Temperature Sensor
76
Filter Heater
76
Loadcell RM 20 | 50
76
Culture Bag Positioning and Operating Volumes
77
Approved Culture Bags
78
Culture Bag Properties
78
Pressures in the Culture Bag
78
Laboratory Energy Sources
78
Mains Connection
78
Aeration and Ventilation
79
Measurement and Control Ranges
79
Rocker Function
79
Aeration and Ventilation
80
Temperature Control
80
Ambient Conditions
80
Acoustic Emissions
81
Communication Interfaces
81
D-LINK 1: Ethernet
81
D-LINK 2: RS-232 Serial Interface
82
D-LINK 3: Profibus DP
84
Data Table
84
Accessories and Consumables
88
Accessories
88
Consumables
88
Sartorius Service
90
Conformity Documents
90
Licensing Information
90
GNU Licensing
90
Appendix
95
Aerating the Cell Culture
95
Schematic Representation of Aeration
95
Overpressure Control
96

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