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Manuals and User Guides for Sage Science PippinHT. We have
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Sage Science PippinHT manual available for free PDF download: Operation Manual
Sage Science PippinHT Operation Manual (78 pages)
DNA Size Selection System
Brand:
Sage Science
| Category:
Laboratory Equipment
| Size: 3 MB
Table of Contents
Table of Contents
3
1 Introduction
6
System Overview
6
How the System Works
7
Collection Strategies
8
Collection Types
9
2 Keys to Successful Collections
10
Common Misconceptions
10
Improving Sample Yield
12
Improving Sample Yield for HMW DNA (High-Pass Protocols)
12
3 Safety and Precautions
13
4 Unpacking and Installation
14
Unpacking the Pippinht
14
Setting up the Pippinht
14
Unpacking Gel Cassette Kits
16
Storage Conditions for Cassettes and Reagents
17
5 Workflow Summary
18
6 Sample Preparation
19
Input Sample Characteristics
19
Preparing DNA Samples for the Pippinht
19
Sample Preparation for Use with an Internal Standard
19
Sample Preparation for Use with an External Marker
20
Recommended Sample Load Guidelines
20
7 Programming a Protocol
21
Overview
21
Protocol Parameters Detail
22
Writing a Size Selection Protocol
24
Using an External Marker
29
8 Optical Calibration
31
9 Preparing a Cassette
33
10 Continuity Test
36
Continuity Test Failure and Troubleshooting
37
11 Loading Samples
39
Sample Loading Best Practices
39
Sample Loading Procedure
40
Loading Less than 12 Samples, and Reusing a Gel Cassette
40
12 Running a Protocol
41
Overview
41
Sample Tracking
42
Gel Cassette Barcodes
42
Sample ID Entry
43
Importing Sample Ids
43
Using the Sample ID Template
45
Log Files
46
Log File Name Format
47
Adding a Prefix to Log File Names
47
Starting, Pausing and Run Completion
48
Monitoring a Run
50
Run Status Indicators
50
Lane Status Indicators
51
Marker Detection Failure Alert
52
Manual Mode
54
13 Log Review
55
Overview
55
Loading a File
56
14 Managing Files
57
Overview
57
File Types
58
Transferring Files
59
15 System Options
61
16 Upgrading Pippinht Software
63
Extracting the Files to a USB Flash Drive
63
Upgrading the Pippin Instrument Software
64
17 Preventative Maintenance - Rinsing Electrodes
67
Electrode Rinse Procedure
67
18 Warranty and Service
68
19 Instrument Specifications
69
20 Gel Cassettes Specifications
70
3% Agarose, 100 Bp-250 Bp, Marker 30G
71
2% Agarose, 100 Bp-600 Bp, Marker 20B
72
Agarose, 250 Bp-1.5 Kb, Marker 15C
73
Agarose, High-Pass, >300Bp-1.5Kb, Marker 15C
74
Agarose, High-Pass >6-10 Kb, Marker 75D
75
Agarose, High-Pass >15-20 Kb, Marker 75E
76
Agarose, High-Pass >30-40 Kb, Marker 75F
77
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