ProteOn XPR36 Manuals

Manuals and User Guides for ProteOn XPR36. We have 1 ProteOn XPR36 manual available for free PDF download: User Manual

ProteOn XPR36 User Manual

ProteOn XPR36 User Manual (288 pages)

Protein Interaction Array System

Brand: ProteOn | Category: Speaker System | Size: 8 MB

Table of Contents

Table of Contents
11
Chapter 1. Introduction

19
- Experiment Workflow
  19
- Proteon Manager Software Features
  20
- Software Overview
  21
  - Instrument Log
    22
  - Status Bar
    23
  - Quick Start Menu
    24
  - Toolbar Icons
    24
  - Proteon Manager Software Options
    25
  - Database Browser Views
    25
  - Start Menu Options
    26
    
    Instrument Shutdown Utility
    27
    
    Instrument Log Extraction Utility
    27
  - Backing up the Database
    28
- Installing Proteon Manager Software
  29
- Starting and Exiting Proteon Manager Software
  39
  - Starting
    39
  - Exiting
    39
- Proteon XPR36 Instrument
  40
  - Starting the Instrument
    40
  - System Shutdown
    40
  - Component Status Leds
    40
  - Status Bar Instrument State Messages
    41
    
    Inactive State
    41
    
    Power up State
    42
    
    Initializing State
    42
    
    Ready State
    42
    
    Running State
    42
    
    Stop State to Ready State
    43
    
    Standby State
    43
    
    Maintenance State
    44
    
    Maintenance Standby State
    44
    
    Shutting down State
    44
    
    Fault State
    44
    
    Flushing State
    45
  - Buffer System
    45
    
    Flushing and Priming the System
    45
    
    Selecting Buffer Bottles
    46
Chapter 2. Protocol Design

49
Chapter 3. Experiments

81
- Required Materials
  81
- Experiment Preparation
  82
- Preparing the Samples
  86
  - Setting the Autosampler Temperature
    87
- Sensor Chips
  88
  - Sensor Chip Types
    89
  - Storage and Temperature Equilibration
    90
  - Opening the Sensor Chip
    90
  - Conditioning the Sensor Chip
    91
    
    GLC, GLM, and GLH Sensor Chip Conditioning
    91
    
    NLC Sensor Chip Conditioning
    93
    
    HTG Sensor Chip Conditioning
    93
  - Initializing the Chip
    94
  - Inserting and Ejecting the Sensor Chip
    94
    
    Chip Loader Leds
    95
  - Setting Sensor Chip Temperature
    96
  - Reusing Chips
    97
  - Viewing Chip Information
    98
- Running Experiments
  99
- Managing Experiments
  105
Chapter 4. Analysis

107
- Tools for Viewing Data
  107
  - Sensorgram Data Windows
    108
  - Changing Sensorgram Appearance
    109
    
    Changing Sensorgram Appearance in a Dataset
    110
  - Changing Sensorgram Views
    112
    
    Showing Interspot Data
    113
    
    Showing Point Minimized Data
    113
    
    Showing Kinetic Results
    113
    
    Viewing Kinetic Data in an Isoaffinity Graph
    114
    
    Visually Comparing Tabular Data in Screening Graphs
    115
    
    Excluding Sensorgrams from Manual Processing
    117
    
    Comparing Data in Multiple Sensorgram Data Windows
    118
    
    Undo
    119
    
    Redo
    119
  - Copying Sensorgram Data Windows
    119
  - Tabulated Data
    119
  - Data Filters and Display Controls
    121
    
    Choosing a Panel Type
    121
    
    Selecting a Protocol Step for Analysis
    121
    
    Grouping Data for Analysis
    121
  - Interaction Display Options
    125
    
    Display Control Grid
    125
- Processing Data
  126
  - Selecting a Range on a Sensorgram
    126
  - Data Processing Tasks
    127
    
    Using the Toolbar
    127
    
    Selecting All Graphs
    127
    
    Copying Data
    127
    
    Processing Multiple Tasks
    128
    
    Aligning the Injection
    128
    
    Aligning the Baseline
    128
    
    Removing Artifacts
    128
    
    Moving a Sensorgram
    129
    
    Performing Reference Subtraction
    129
    
    Displaying Real-Time Data
    131
    
    Deleting Report Point and User-Defined Columns
    131
    
    Displaying and Hiding Markers
    131
    
    Remove Processing
    132
    
    Switching to Raw Dataset
    132
  - Processing EV Correction Data
    133
    
    Working with EVC Calibration Plots
    135
  - Creating a Dataset
    136
  - Renaming a Dataset
    136
  - Deleting a Dataset
    137
- Analyzing Data
  138
  - Analysis Datasets
    138
    
    Parameter Types
    138
    
    Specifying Report Points
    139
    
    Specifying Report Points on Concatenated Data
    140
    
    Computing Difference between Report Points
    140
    
    Viewing Report Points from a Dataset
    141
    
    Importing Report Points from Other Datasets
    141
    
    Saving Report Points to an External File
    142
    
    Adding a Report Points Column
    142
    
    Deleting Report Point Columns
    142
    
    Filtering and Sorting Analysis Results
    142
    
    Adding a User-Defined Column
    142
    
    Deleting a User-Defined Column
    143
    
    Renaming an Analysis Set
    143
    
    Deleting an Analysis Set
    144
  - Analysis Wizards
    144
    
    Kinetic Analysis Wizard
    145
    
    Equilibrium Analysis Wizard
    150
    
    Concentration Analysis Wizard
    152
  - Ungrouping Analysis Results
    156
  - Filtering Analysis Results
    156
  - Sorting Analysis Results
    158
- Saving and Exporting Data
  159
  - Exporting Data to a File
    159
  - Exporting Analysis Data
    160
  - Copying and Pasting Data to a File
    161
  - Exporting Experiments to a File
    162
- Reports
  163
  - Customizing and Printing Reports
    164
  - Exporting a Report
    165
Chapter 5. Maintenance

167
- Maintenance and Cleaning Chips
  167
  - Maintenance Chips (MNT)
    168
  - Cleaning Chips (CLN)
    169
- Instrument Maintenance
  169
  - Safety
    169
  - Scheduled Maintenance Wizards
    170
  - Maintenance Protocol Solutions
    171
- Maintenance Wizard Instructions
  172
  - Prime
    172
  - Weekly
    172
  - Postexperiment
    173
  - Clean MCM
    173
  - Syringe Maintenance
    174
    
    Starting the Syringe Wizard
    175
    
    Cleaning Syringes
    179
    
    Replacing the Syringes
    179
    
    Resuming the Wizard
    180
  - OQ Protocol
    183
- Shutdown Procedures
  184
  - Immediate Shutdown
    184
  - Long-Term Shutdown
    185
  - Software-Only Shutdown
    186
  - Exiting Proteon Manager Software and the Instrument
    186
  - Emptying the Collection Tank
    187
Chapter 6. Proteon System Troubleshooting

189
- Common Concerns
  189
- Nonspecific Binding
  190
  - Background
    190
  - Addressing the Causes of NSB
    191
- Firewall Settings
  192
- High Throughput Screening Analyses
  193
Chapter 7. Security Edition

195
- Background on U.S. FDA Part 11 Regulations (21 CFR Part 11)
  195
- Standard Mode Vs. Secure Mode
  196
- System Requirements
  196
- Installing and Starting Proteon Manager Software, Security Edition
  196
- Enabling and Disabling Secure Mode
  197
- Users, Passwords, and User Levels
  198
  - User Level Restrictions
    198
  - User Access by Function
    198
  - User Authentication
    199
- File Security and Validation
  200
  - Importing/Exporting Secure Files
    200
- Proteon Manager Software Logs
  200
- Secure Documents
  200
- Signed Files
  201
- Signed Datasets and Analysis Sets
  202
- Audit Trail
  202
  - Viewing the Audit Trail
    204
- Protected Directories
  205
- Logging off
  205
- Changing Data Ownership
  206
Proteon XPR36 Instrument and Peripherals

207
- Instrument Front View
  208
- Instrument Side View
  209
  - Instrument Leds
    210
  - Chip Loader and Chip Eject Button
    211
  - Buffer Control Buttons
    211
  - Serial Numbers
    212
  - Cables and Power Switch
    213
  - Ventilation
    213
- Inside the Instrument
  215
  - Buffer System
    215
  - Microfluidics System Components
    216
  - Autosampler
    217
  - Thermal Platform
    217
  - Needle Holder with Sampling Needles
    218
  - Changing the Needle Holder and Needles
    218
  - Wash Station
    220
  - Sample Holders
    221
    
    Sample Holder Sensors
    222
  - Syringe Pumps
    222
    
    Multichannel Module
    223
  - Optical Detection System
    223
  - Reservoir, Overflow Sensor, and Drain Pump
    224
- Computer and Display
  224
  - HASP Keys
    225
Specifications and Requirements

227
- System Specifications
  227
  - Detection System
    227
  - Detection Limits
    227
  - Working Ranges (Typical Values)
    228
  - Fluidics
    228
  - Physical
    228
- Installation Requirements
  229
  - Electrical Requirements
    229
  - Bench Requirements
    229
  - Recommendations
    229
  - Environmental Requirements
    230
  - Chemical Compatibility
    230
Surface Plasmon Resonance

231
- Biomolecular Applications
  231
- Interaction Analysis
  232
  - Ligand Immobilization Steps
    232
  - Analyte Interaction Steps
    232
- Ligand Immobilization Process
  233
  - Immobilization Chemistries
    233
    
    Direct, Covalent Coupling
    233
    
    Noncovalent Coupling
    233
  - Activation Step (Amine Coupling)
    234
  - Immobilization Step
    234
- Determining the Optimum Ph for Immobilization
  237
  - Deactivation and Stabilization Steps
    238
  - Determining the Extent of Ligand Immobilization
    239
- Analyte Interaction Process
  240
  - Analyte Step
    240
  - Regeneration Step
    242
Protocol Development Kits

243
Ordering Information

245
- How to Order
  245
  - Payment
    245
  - Phone-In Hours
    245
  - Information Needed
    245
  - Materials and Supplies
    246
Security Edition Configuration Guide

251
Glossary

265
Index

277

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