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102-837-000
PacBio 102-837-000 Manuals
Manuals and User Guides for PacBio 102-837-000. We have
1
PacBio 102-837-000 manual available for free PDF download: Operation Manual
PacBio 102-837-000 Operation Manual (49 pages)
Cluster generator and short-read sequencer
Brand:
PacBio
| Category:
Laboratory Equipment
| Size: 2 MB
Table of Contents
Table of Contents
3
Before You Begin
5
Flow Cell and Main Instrument Connector Assembly (MICA) Configuration
5
Flow Cell Configuration
5
MICA Configuration
6
A) Connecting MICA Instrument Connection Side to Instruments
6
B) Disconnecting MICA Instrument Connection Side from Instruments
6
Powering Instruments on
6
Safety Guidelines
7
General Best Practices
7
DNA Library Input
7
Multiplexing
7
Library Loading Concentration and Control Spike-In Guidelines
7
Reagent and Sample Handling
8
Clustering on the Cluster Generator
8
Cluster Generator
9
Clustering Workflow
10
Required Materials and Equipment
11
Clustering Procedure
12
Thawing Clustering Reagents
12
Preparing Instrument Wash Reagent
12
Denaturing and Diluting the DNA Library
12
Onso Clustering Reagent Plate Preparation
15
Cluster Generator Setup and Run
16
Post-Run Wash
23
Instrument Washes
26
Long-Term Shutdown
28
Sequencing
29
Onso System Short-Read Sequencer
29
Sequencing Workflow
30
Required Materials and Equipment
30
Sequencing Procedure
31
Thawing Sequencing Reagent Pack Thaw
31
Optional Custom Primer Addition
31
Sequencing Set up
34
Unloading the Sequencing Instrument
41
Kitting and Discarding the Reagent Pack
42
Instrument Washes
43
Long-Term Shutdown
46
Run Management
46
System Checks
47
Troubleshooting
49
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