Molecular Devices Axoclamp 900A Manuals

Manuals and User Guides for Molecular Devices Axoclamp 900A. We have 1 Molecular Devices Axoclamp 900A manual available for free PDF download: User Manual

Molecular Devices Axoclamp 900A User Manual

Molecular Devices Axoclamp 900A User Manual (213 pages)

Computer-controlled Microelectrode Amplifier

Brand: Molecular Devices | Category: Amplifier | Size: 2 MB

Table of Contents

Table of Contents
7
Chapter 1: Introduction

15
Chapter 2: Installation and Basic Operation

19
- Installation
  19
  - Check List
    19
  - Installing Hardware
    20
  - Installing the Axoclamp 900A Commander
    20
- Input/Output Connections
  22
  - Front Panel
    22
  - Rear Panel
    23
- Overview
  25
  - Axoclamp 900A Commander Interface Overview
    26
  - Toolbar
    26
  - Meters
    27
  - Mode Buttons
    27
  - Mode Tabs
    28
  - Scaled Output Tabs
    29
  - Setting Parameters in the Axoclamp 900A Commander
    29
  - Toolbar Buttons in the Axoclamp 900A Commander
    32
  - Communication with the Axoclamp 900A
    32
- Model Cells
  33
  - The CLAMP-1U Model Cell
    33
  - The MCO-2U Model Cell
    35
  - Installation of Model Cells
    35
- Power Supply
  35
  - Supply Voltage Selection
    35
  - Changing the Fuse
    36
  - Glitches
    36
- Grounding and Hum
  37
Chapter 3: Setup and Tutorials

39
Chapter 4: Guide to Electrophysiological Recording

73
- General Advice
  73
  - Chamber Design
    73
  - Perfusion
    74
  - Mechanical Stability
    74
  - Optics
    75
  - Bath Electrode
    75
  - Interfacing a Computer
    76
- Noise
  76
  - Measurement of Noise
    76
  - Sources of Noise
    77
- Bath Error Potentials
  79
- Headstages
  84
  - HS-9A Series
    84
  - VG-9A Series
    84
  - Current Clamp Circuit
    85
  - Mounting the Headstage
    86
  - Bath Connection
    86
  - Static Precautions
    86
  - Acoustic Pick-Up
    86
  - Compatibility of the HS-2 and HS-2A with the Axoclamp 900A
    87
  - General Characteristics
    87
  - The Meaning of H
    88
  - Guidelines for Selecting a Headstage
    89
  - Headstage Connection
    91
  - Interchangeability
    91
  - Cleaning
    91
  - Input Leakage Current
    91
  - Input Resistance
    92
- Holders
  92
  - Holder Design
    92
  - Optional Ag/Agcl Pellets
    94
  - Holder Use
    94
  - Holder Maintenance
    95
- Sharp Microelectrode Recording
  96
  - Microelectrode Properties
    96
  - Filling Solutions
    99
  - Impaling Cells
    100
- Theory of Recording Modes
  101
  - Current-Clamp Mode
    101
  - Discontinuous Current Clamp (DCC) Mode
    102
  - Discontinuous Single-Electrode Voltage Clamp (Dsevc) Mode
    105
  - Noise in DCC and Dsevc Modes
    110
  - TEVC Mode
    112
- Filters
  121
  - 3 Db Frequency
    121
  - Types of Filters
    122
- Blanking
  124
- Current Measurement
  125
- Virtual-Ground Current Measurement
  126
Chapter 5: Commander Reference

129
- Always on Top
  129
- Bridge Balance
  129
  - Bridge Balance Overview
    130
  - Bridge Balance in the Bath
    132
  - Bridge Balance in the Cell
    132
- Buzz
  133
- Clear
  134
- Configure Softpanel
  134
  - Default Softpanel Settings
    135
  - How to Configure Softpanel
    135
- Detect Oscillation
  137
  - Current Clamp
    137
  - Voltage Clamp
    138
- Current Injection Resistor, R
  138
- Holding
  138
  - How to Use Holding
    139
- Keyboard Shortcuts
  139
  - Hot Keys
    139
  - Shortcut Keys
    139
- Load Configuration
  142
- Mode Switching
  143
  - Connecting an External Command Signal
    143
  - Automatic Mode Switching
    144
  - Additional Note
    144
- Monitor Scope
  145
- Options
  145
- Options / General
  145
  - External Command Sensitivity
    146
  - Lowpass Filter Type
    147
  - Sync Output
    147
  - Auxiliary Headstage Information
    148
  - Miscellaneous
    148
- Options / Auto
  149
  - Switch to from I-Clamp to Dsevc / TEVC
    149
  - Return to Current Clamp
    150
- Options / Audio
  151
  - Mute
    152
  - Volume
    152
  - Audio Mode
    152
  - Audio Signal
    153
- Options / Quick Select
  153
- Options / about
  154
- Output Offset
  154
  - How to Use Output Offset
    155
  - Additional Notes
    156
- Pipette Capacitance Neutralization
  156
- Pipette Offset
  158
  - How to Use Pipette Offset
    159
  - Additional Notes
    159
- Pulse
  159
  - How to Use Pulse
    160
  - Additional Notes
    160
- Quick Select
  160
  - Configuration Files
    160
  - Executable Files
    161
- Reset to Program Defaults
  161
- Resize Window
  161
- Save Configuration
  161
- Scaled Output
  162
  - Voltage Clamp
    162
  - Current Clamp
    163
  - Auxiliary Headstages
    164
  - Telegraphs
    164
  - Conditioning the Scaled Output
    164
  - Highpass Filter
    165
  - Gain
    165
  - Lowpass Filter
    166
- Seal Test
  167
  - How to Use Seal Test
    167
  - Additional Notes
    167
- Select Device
  168
  - Axoclamp Tab
    168
  - Softpanel Tab
    169
- Slow Current Injection
  170
  - How to Use Slow Current Injection
    171
- Telegraphs
  171
- Tuning
  172
  - How to Use Tuning
    172
  - Additional Notes
    173
- V-Clamp Control
  173
  - Gain
    173
  - Lag
    173
  - DC Restore
    174
Chapter 6: Troubleshooting

175
- Voltage Clamp Problems
  176
  - Dsevc
    176
  - Tevc
    176
  - All Voltage-Clamp Modes
    177
- Space Clamp
  178
- Noise
  180
Chapter 7: Specifications

181
- Functional Specification
  181
  - Instrument Usage
    181
  - Main Unit
    181
  - Headstage
    182
  - Axoclamp 900A Headstages
    182
  - Output Compliance
    182
  - Pipette Capacitance Neutralization
    182
  - Oscillation Detection and Correction
    183
  - Sample Rate
    183
  - DCC Mark/Space
    183
  - Phase Lag
    183
  - Dsevc Gain
    183
  - TEVC Gain
    183
  - DC Restore
    184
  - Buzz
    184
  - Clear (+/-)
    184
  - Step Activate
    184
  - Blank Activate
    184
  - Scope
    185
  - Options
    185
  - Internally Generated Signals
    186
  - External Command Inputs
    187
  - External Current Outputs (2 Off)
    187
  - Auxiliary Inputs & Outputs (2 Off)
    187
  - Scaled Outputs
    188
  - Metering (4 Off)
    188
  - Audio Monitor
    189
- Performance Specification
  189
  - I-Clamp (Channels 1 & 2)
    189
  - DCC (Channel 1)
    189
  - HVIC (Channel 2)
    190
  - Dsevc (Channel 1)
    190
  - TEVC (Channels 1 & 2 Together)
    190
Appendix A: References

193
Appendix B: Circuit Diagrams Request Form

197
Appendix C: Important Safety Information

199
Appendix D: Polarity Conventions

207
Appendix E: Glossary

209
- Index
  211

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