Micromass Quattro Ultima Manuals

Manuals and User Guides for Micromass Quattro Ultima. We have 1 Micromass Quattro Ultima manual available for free PDF download: User Manual

Micromass Quattro Ultima User Manual

Micromass Quattro Ultima User Manual (227 pages)

Brand: Micromass | Category: Laboratory Equipment | Size: 4 MB

Table of Contents

Method
2
Table of Contents
3
Hardware Specifications

11
- Dimensions
  11
- Weights
  11
  - Lifting and Carrying
    12
- Power
  13
- Environment
  13
- Water Cooling
  13
- Exhausts
  13
  - Rotary Pumps
    13
  - API Gas Exhaust
    13
- Nitrogen
  14
- CID Gas
  14
Instrument Description

15
- Overview
  15
- Vacuum System
  16
- Ionisation Techniques
  17
  - Atmospheric Pressure Chemical Ionisation
    17
  - Electrospray
    17
    
    Nanoflow Electrospray
    17
- Sample Inlet
  17
- MS Operating Modes
  18
- MS-MS Operating Modes
  18
- Data System
  22
- Front Panel Connections
  23
  - Desolvation Gas and Probe Nebuliser Gas
    23
  - Capillary / Corona
    23
  - ESI / Apci
    23
- Front Panel Controls and Indicators
  24
  - Status Display
    24
    
    Operate LED
    24
    
    Vacuum LED
    24
  - Flow Control Valves
    25
  - Divert / Injection Valve
    25
- Rear Panel Connections
  26
  - Event out
    26
  - Contact Closure in
    26
  - Analog Channels
    26
  - Mux
    27
  - Data System
    27
  - Water
    28
  - Nitrogen Gas in
    28
  - Exhausts
    29
  - CID Gas
    29
  - Power Cord
    29
  - Mains Switch
    29
  - Fuses
    29
  - Rotary Control
    29
  - ESD Earth Facility
    29
- Internal Layout
  30
  - Electronics
    30
  - Mechanical Components
    32
Routine Procedures

33
- Start up Following a Complete Shutdown
  33
  - Preparation
    33
  - Pumping
    36
  - Measuring the Analyser Pressure
    37
  - Using the Instrument
    37
- Start up Following Overnight Shutdown
  37
- Automatic Pumping and Vacuum Protection
  42
  - Overview
    42
    
    Protection
    42
    
    Transient Pressure Trip
    42
    
    Power Failure
    43
    
    Pump Fault
    43
- Tuning
  44
- Calibration
  44
- Data Acquisition
  44
- Data Processing
  44
- Setting up for MS-MS Operation
  44
  - Parent Ion Selection
    44
  - Fragmentation
    45
- Shutdown Procedures
  46
  - Emergency Shutdown
    46
  - Overnight Shutdown
    46
  - Complete Shutdown
    47
- Automatic Start up and Shutdown
  48
Tuning

55
- Overview
  55
- The Tune Page
  56
- Printing Tune Information
  56
- Experimental Record
  56
- Saving and Restoring Parameter Settings
  56
- Modifying the Peak Display
  58
- Changing the Display
  60
  - Customise Plot Appearance
    60
  - Trace
    61
  - Intensity
    61
  - Grid
    61
- Autotune
  62
- Ion Mode
  63
- Scope Parameters
  64
- Gas Controls
  64
- Ramp Controls
  64
- Resetting the Zero Level
  65
- Controlling Readbacks
  66
- Changing Tune Parameter Settings
  67
- Source Voltages
  67
Data Acquisition

69
- Starting an Acquisition
  69
  - Starting an Acquisition from the Tune Page
    69
    
    Parameters
    70
  - Multiple Samples
    71
    
    Automated Analysis of Sample List
    72
    
    Process
    72
- Monitoring an Acquisition
  74
- Instrument Data Thresholds
  75
  - Maxent
    76
  - Profile Data
    76
  - Centroid Data
    76
  - SIR Data
    76
  - Ion Counting Threshold
    77
  - Profile Data - Spike Removal
    78
  - Analog Data
    79
- System Manager
  79
- Stopping an Acquisition
  80
- The Function List Editor
  80
  - Introduction
    80
  - The Function List Editor Toolbar
    82
  - Adding a New Function
    82
  - Modifying an Existing Function
    83
  - Copying an Existing Function
    83
  - Removing a Function
    83
  - Changing the Order of Functions
    83
  - Setting a Solvent Delay
    84
  - Analog Channels
    84
  - Saving and Restoring a Function List
    85
  - Setting up a Full Scan Function
    86
    
    Cone Voltage
    86
    
    Mass (M/Z)
    86
    
    Method
    86
    
    Apci Probe
    87
    
    Scan Duration (Secs)
    87
  - Setting up a SIR Function
    88
    
    Channels
    88
    
    Method
    89
    
    Retention Window
    89
  - Setting up MS-MS Scanning Functions
    90
    
    Mass
    90
    
    Collision Energy
    92
  - Setting up a MRM Function
    93
  - Setting up a Survey Function
    93
    
    Survey and MSMS Template Pages
    94
    
    MS to MSMS Switching
    95
    
    MSMS to MS Switching
    97
    
    Including and Excluding Masses
    98
    
    Monitoring Acquisitions
    99
Mass Calibration

101
- Introduction
  101
- Overview
  102
  - Calibration Types
    102
  - The Calibration Process
    103
- Electrospray
  103
  - Introduction
    103
  - Preparing for Calibration
    104
    
    Reference Compound Introduction
    104
    
    Tuning
    104
    
    Instrument Threshold Parameters
    105
  - Calibration Options
    106
    
    Removing Current Calibrations
    106
    
    Selecting the Reference File
    106
  - Selecting Parameters
    107
    
    Automatic Calibration Check
    107
    
    Calibration Parameters
    108
    
    Mass Measure Parameters
    109
  - Performing a Calibration
    110
    
    Acquisition Parameters
    112
    
    Starting the Calibration Process
    114
  - Checking the Calibration
    116
    
    Calibration Failure
    118
    
    Incorrect Calibration
    120
    
    Manual Editing of Peak Matching
    121
    
    Saving the Calibration
    121
    
    Verification
    122
  - Electrospray Calibration with PEG
    124
- Atmospheric Pressure Chemical Ionisation
  125
  - Introduction
    125
  - Preparing for Calibration
    126
    
    Reference Compound Introduction
    126
    
    Tuning
    126
  - Calibration Options
    126
    
    Removing Current Calibrations
    126
    
    Selecting Reference File
    126
  - Selecting Calibration Parameters
    126
  - Performing a Calibration
    127
    
    Static Calibration
    127
    
    Scanning Calibration and Scan Speed Compensation
    132
  - Calibration Failure
    135
  - Incorrect Calibration
    136
  - Manual Editing of Peak Matching
    137
  - Saving the Calibration
    137
  - Manual Verification
    138
Electrospray

141
- Introduction
  141
  - Post-Column Splitting
    144
  - Megaflow
    145
    
    Changing between Flow Modes
    145
- Operation
  146
  - Checking the ESI Probe
    147
  - Obtaining an Ion Beam
    148
    
    Tuning and Optimisation
    148
  - Megaflow Hints
    154
  - Removing the Probe
    154
- Sample Analysis and Calibration
  155
  - General Information
    155
    
    Typical es Negative Ion Samples
    156
    
    Typical es Positive Ion Samples
    156
- Chromatographic Interfacing
  157
  - LC-MS Sensitivity Enhancement
    158
Nanoflow Electrospray

159
- Overview
  159
- Installing the Interface
  160
- Operation of the Camera System
  163
- Using the Microscope
  163
- Glass Capillary Option
  164
  - Restarting the Spray
    165
- Nano-LC Option
  166
  - Installation
    166
  - Operation
    167
- Changing Options
  168
Atmospheric Pressure Chemical Ionisation

169
- Introduction
  169
- Preparation
  170
  - Checking the Probe
    171
- Obtaining a Beam
  172
- Calibration
  173
- Hints for Sample Analysis
  174
  - Tuning for General Qualitative Analysis
    174
  - Specific Tuning for Maximum Sensitivity
    174
    
    Corona Current
    175
    
    Desolvation Gas
    175
    
    Probe Position
    175
    
    Probe Temperature
    175
- Removing the Probe
  176
Maintenance and Fault Finding

177
- Introduction
  177
- Cooling Fans and Air Filters
  177
- The Vacuum System
  178
  - Vacuum Leaks
    179
  - Pirani Gauge
    179
  - Active Inverted Magnetron Gauge
    179
  - Gas Ballasting and Rotary Pump Oil Recirculation
    180
  - Oil Mist Filter
    181
  - Foreline Trap
    181
  - Rotary Pump Oil
    182
- The Source
  183
  - Overview
    183
  - Cleaning the Cone Gas Nozzle and Sample Cone
    184
  - Removing and Cleaning the Ion Block
    188
  - Removing and Cleaning the Ion Tunnel Assembly
    192
  - Reassembling and Checking the Source
    194
  - The Discharge Pin
    195
- The Electrospray Probe
  196
  - Overview
    196
  - Replacement of the Stainless Steel Sample Capillary
    198
- The Apci Probe
  200
  - Cleaning the Probe Tip
    200
  - Replacing the Probe Tip Heater
    201
  - Replacing the Fused Silica Capillary
    202
- The Analyser
  204
- The Detector
  204
- Electronics
  205
  - Fuses
    205
    
    Analog PCB
    205
    
    Power Backplane #2
    205
    
    Power Sequence PCB
    205
    
    Pumping Logic PCB
    205
    
    Rear Panel
    205
    
    RF Power PCB
    205
- Fault Finding Check List
  206
  - No Beam
    206
  - Unsteady or Low Intensity Beam
    206
  - Ripple
    206
  - High Noise Level in MRM Analyses
    207
    
    Chemical Noise
    207
    
    Electronic Noise
    208
  - High Back Pressure
    208
  - General Loss of Performance
    209
- Cleaning Materials
  210
- Preventive Maintenance Check List
  211
  - Weekly
    211
  - Monthly
    211
  - Three-Monthly
    211
  - Four-Monthly
    211
Reference Information

213
- Overview
  213
- Editing a Reference File
  214
- Positive Ion
  215
  - Horse Heart Myoglobin
    216
  - Polyethylene Glycol
    216
    
    Peg + Nh4
    216
  - Sodium Iodide and Caesium Iodide Mixture
    217
  - Sodium Iodide and Rubidium Iodide Mixture
    217
- Negative Ion
  218
- Preparation of Calibration Solutions
  220

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