LI-COR 4300 Manuals

LI-COR 4300 Manuals

Manuals and User Guides for LI-COR 4300. We have 1 LI-COR 4300 manual available for free PDF download: Manual

LI-COR 4300 Manual

LI-COR 4300 Manual (48 pages)

DNA Analyzer

Brand: LI-COR | Category: Measuring Instruments | Size: 0 MB

Table of Contents

Table of Contents
3
Section 1. Plate Organization

5
Section 2. Gel Preparation and Electrophoresis

9
- Choosing Plates and Spacers
  9
- Choosing a Gel
  9
- Plate Assembly
  10
- KB Plus Gel Preparation
  14
- Preparing Gel Solutions from Other Manufacturers
  15
- Pouring a 6.5% KB Plus Gel
  18
- Pre-Electrophoresis Preparation
  21
- Starting Runs
  26
- Sample Loading
  26
- Disassembly
  27
- Cleanup
  28
Section 3. PCR Protocols

31
- Dntp Recommendation
  31
- PCR Optimization
  31
- Multiplexing Loci
  31
- Microsatellite Optimization
  32
- Lab Organization
  34
- Preparing Genomic DNA
  35
- Size Standards
  35
- Cycling Programs
  37
- Tailed Primers
  39
- Labeled Primers
  41
- Troubleshooting
  43
  - Calculation of Oligonucleotide Concentration (Nmol) Given Optical Density (O.D.)
    46
  - Protocol for 96-Well Paper Combs
    47

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