User Manuals: Invitrogen EVOS M5000 Cell Imaging System

Manuals and User Guides for Invitrogen EVOS M5000 Cell Imaging System. We have 1 Invitrogen EVOS M5000 Cell Imaging System manual available for free PDF download: User Manual

Invitrogen EVOS M5000 User Manual (160 pages)

Imaging System

Brand: Invitrogen | Category: All in One Printer | Size: 5 MB

Table of Contents
3
CHAPTER 1 about this Guide

9
- Audience
  9
- User Attention Words
  9
- Safety Alert Words
  9
CHAPTER 2 Product Information

10
- Product Description
  10
- EVOS ™ M5000 Imaging System
  10
- EVOS ™ M5000 Software
  10
- Product Use
  11
- Standard Items Included
  11
- EVOS ™ M5000 Imaging System
  11
- EVOS ™ M5000 Accessories Kit
  11
- EVOS ™ M5000 Imaging System User Documentation
  12
- Instrument Exterior Components and Mechanical Controls
  13
- Front View
  13
- Rear View
  14
- Graphical User Interface (GUI)
  15
- GUI Layout
  15
CHAPTER 3 Installation

16
CHAPTER 4 Capture Images

27
- Overview
  27
- Workflow
  27
- Capture Tab
  28
- Capture Images in a Single Channel
  28
- Select Objective and Light Source
  28
- Adjust Brightness
  29
- Focus on the Sample
  30
- Optional: Adjust Display Settings
  31
- Find a Region of Interest
  31
- Capture Images for each Channel
  32
- Capture Images in Multiple Channels
  34
- Capture Multiple Channels Automatically
  34
CHAPTER 5 Measure, Annotate, and Analyze Captured Images

36
- Display Settings and Analysis Tools
  36
- Adjust Image Display Settings
  37
- Display Grid
  38
- Display Scale Bar
  39
- Align Channels
  40
- View Pixel Intensity Histogram
  41
- Display Histogram
  41
- Add Measurements and Annotations
  42
- Analyze Cell Culture
  44
- Analysis Tools
  44
- Count Cells - Auto Count
  45
- Perform Auto Count
  45
- Count Cells - Manual Count
  51
- Perform Manual Count
  51
- Measure Confluence
  54
- Confluence Tool
  54
- Calculate Transfection Efficiency
  58
CHAPTER 6 Save Captured Images

62
- Save
  62
- Save Images Manually
  62
- Quick Save Images
  63
- (Optional) Enable Quick Save
  63
CHAPTER 7 Capture Time Lapse Images

65
CHAPTER 8 Capture Z-Stack

74
- Z-Stack Tool
  74
- Capture Z-Stack Images
  74
CHAPTER 9 Stage View

76
- Overview
  76
- Workflow
  77
- Set Stage Origin
  78
- Mark Pin Locations
  81
- Find Pins Using the Find Target Screen
  81
- Load Pin Map
  84
- Update the Target Pin Location
  84
CHAPTER 10 Review and Analyze Saved Images

85
- Review Images
  86
- Configure Display Settings
  88
- Adjust Display Settings for Saved Images
  88
- Display Grid
  89
- Display Scale Bar
  89
- View Pixel Intensity Histogram
  89
- Display Histogram
  89
- Add Measurements and Annotations to Saved Images
  90
- Analyze Cell Culture Using Saved Images
  90
- Save Analysis Results
  92
- Save
  92
- Batch Analysis
  94
- Batch Analysis Function
  94
- Save Current Analysis Settings
  94
- Run Batch Analysis
  94
CHAPTER 11 Configure Instrument Settings

96
- Overview
  96
- Settings Tab
  96
- Adjust Objective Settings
  98
- Assign Objectives
  98
- Calibrate Objective Magnification
  99
- Delete Objectives
  99
- Calibrate White Balance
  100
- Set Saturated Pixel Options
  101
- Highlight Saturated Pixels
  101
- General Settings
  101
- General Settings Options
  101
- Define Capture All Checkbox Behavior
  101
- Define Display Settings Toolbar Behavior
  102
- Show Align Channels Tool
  102
- Configure Network Settings
  102
- Connect to a Wi-Fi Network
  103
- Map Network Drive
  103
- Service
  105
- Copy Error Logs
  105
- Set Date and Time
  105
- Update from Cloud
  106
- Update from USB
  106
CHAPTER 12 Instrument Care and Maintenance

107
- General Care
  107
- Objective Lens Care
  108
- Stage Care
  108
- Decontamination Procedures
  108
- Change EVOS ™ LED Light Cubes
  109
- Change LED Light Cube
  109
- Change the Objectives
  110
- Procedure for Objective Change
  110
- Calibrate the Objectives
  112
- Calibrate Objective Magnification
  112
APPENDIX A Troubleshooting

114
- Image Quality Issues
  114
- Stage View Issues
  115
- Software Interface Issues
  115
- Mechanical Issues
  116
APPENDIX B Graphical User Interface (GUI)

117
- Capture Tab
  117
- Review Tab
  126
- Auto Count Controls
  128
- Manual Count Controls
  129
- Cell Culture - Confluence Controls
  130
- Cell Culture - Transfection Efficiency Controls
  131
- Settings
  132
APPENDIX C System Overview

135
- Technical Specifications
  135
- Physical Characteristics
  135
- Hardware
  135
- Operation Principles and Technical Overview
  136
- LED Illumination
  136
- LED Light Cubes
  136
APPENDIX D EVOS ™ Image Analysis

138
- EVOS Image Analysis
  138
- Gallery
  139
- Edit Image
  139
- Adjust Tab
  140
- Analyze Tab
  141
APPENDIX E Glossary

142
- Software Controls and Functions
  142
- Image Files
  143
- Microscope Controls and Optics
  145
- Stage View Feature
  146
APPENDIX F Safety

147
- Symbols on this Instrument
  147
- Standard Safety Symbols
  147
- Location of Labels
  149
- Control and Connection Symbols
  149
- Conformity Symbols
  149
- Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
  150
- Instrument Safety
  150
- General
  150
- Physical Injury
  151
- Electrical Safety
  152
- Cleaning and Decontamination
  153
- Instrument Component and Accessory Disposal
  153
- Safety and Electromagnetic Compatibility (EMC) Standards
  153
- Safety Standards
  154
- EMC Standards
  154
- Environmental Design Standards
  155
- Chemical Safety
  156
- Biological Hazard Safety
  157
APPENDIX G Documentation and Support

158
- Customer and Technical Support
  158
- Limited Product Warranty
  158

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User Manuals: Invitrogen EVOS M5000 Cell Imaging System

Invitrogen EVOS M5000 User Manual (160 pages)

Table of Contents

CHAPTER 1 about this Guide

CHAPTER 2 Product Information

CHAPTER 3 Installation

CHAPTER 4 Capture Images

CHAPTER 5 Measure, Annotate, and Analyze Captured Images

CHAPTER 6 Save Captured Images

CHAPTER 7 Capture Time Lapse Images

CHAPTER 8 Capture Z-Stack

CHAPTER 9 Stage View

CHAPTER 10 Review and Analyze Saved Images

CHAPTER 11 Configure Instrument Settings

CHAPTER 12 Instrument Care and Maintenance

APPENDIX A Troubleshooting

APPENDIX B Graphical User Interface (GUI)

APPENDIX C System Overview

APPENDIX D EVOS ™ Image Analysis

APPENDIX E Glossary

APPENDIX F Safety

APPENDIX G Documentation and Support

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