illumina HiSeq 2000 Manuals

illumina HiSeq 2000 Manuals

Manuals and User Guides for illumina HiSeq 2000. We have 1 illumina HiSeq 2000 manual available for free PDF download: System Manual

illumina HiSeq 2000 System Manual

illumina HiSeq 2000 System Manual (76 pages)

Brand: illumina | Category: Recording Equipment | Size: 0 MB

Table of Contents

Table of Contents
6
Chapter 1 Overview

8
Chapter 2 Getting Started

15
- Start the Hiseq 2000
  15
- Customize System Settings
  15
- View and Send Instrument Data
  16
- User-Supplied Consumables
  17
Chapter 3 Sequencing in Hiseq V4 Mode

18
- Introduction
  18
- Prepare Reagents
  19
- Enter Run Parameters
  20
- Load and Prime Reagents
  23
- Load the Sequencing Flow Cell
  27
- Monitor the Run
  30
- Unload Reagents
  30
- Perform a Water Wash
  31
Chapter 4 Sequencing in Truseq V3 Mode

33
- Introduction
  33
  - Prepare Reagents for Read 1
    34
- Enter Run Parameters
  37
- Load and Prime Reagents
  40
- Load the Sequencing Flow Cell
  44
- Monitor the Run
  46
  - Prepare Reagents for Read 2
    47
- Unload Reagents
  50
- Perform a Water Wash
  50
Chapter 5 Maintenance

52
- Introduction
  52
- Perform a Maintenance Wash
  52
- Idle the Instrument
  54
- Shut down the Instrument
  54
Appendix A Troubleshooting

56
- Possible Run Setup Problems
  56
- Stop and Resume a Run
  57
- Pause a Run
  59
- Stagger Runs on Flow Cell a and Flow Cell B
  59
- Split SBS Kits
  60
- Primer Rehybridization
  61
Appendix B Real-Time Analysis

63
- Real-Time Analysis Overview
  63
- Real-Time Analysis Workflow
  64
- Monitor Run Metrics
  66
Appendix C Output Files and Folders

68
- Sequencing Output Files
  68
- Output Folder Structure
  69
- Tile Numbering
  69
- Thumbnail Images
  70
- Index
  71
- Technical Assistance
  75

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