User Manuals: Harvard Bioscience BTX Hybrimune System

Manuals and User Guides for Harvard Bioscience BTX Hybrimune System. We have 1 Harvard Bioscience BTX Hybrimune System manual available for free PDF download: User Manual

Harvard Bioscience BTX Hybrimune User Manual

Harvard Bioscience BTX Hybrimune User Manual (52 pages)

Hybridoma Production System

Brand: Harvard Bioscience | Category: Medical Equipment | Size: 1 MB

Table of Contents

Cloning Medium
2
Table of Contents
3
Warranty Information
6
Safety Information
7
Hybrimune™ System Components and Set-Up

8
- Introduction
  8
- The Hybrimune™ Waveform Generator
  8
- Figure 1: Hybrimune System
  8
- The Large-Volume Fusion Chamber
  9
- Cytofusion® Medium
  9
- Figure 2: Large Fusion Chambers
  9
- Figure 3: Cytofusion® Medium
  9
- Setting up the Hybrimune™ System
  10
- Waveform Generator Front Panel Display
  10
- Waveform Generator Interface Software Installation
  11
  - Set-Up Using a USB-To-Serial Converter
    11
- System Test
  12
- Figure 5: User Interface Application Screen
  12
- Figure 6: Last Protocol Log
  13
- Elementary Verification Tests
  14
- The Waveform Generator Interface Software
  14
  - The Toolbar
    15
  - Tools Area
    15
  - Status Area
    15
  - Last Protocol Log Window
    16
  - Control Panel Area
    16
  - Running a Protocol
    17
  - File Management
    17
- Programming Waveform Protocols
  18
  - Programming the Protocol
    18
Producing Fusion Products Using Hybrimune

19
- Cell Preparation before Electrofusion Day
  19
  - Myeloma Cells
    19
  - Available Myelomas or Heteromyelomas
    20
    
    Human
    20
    
    Mouse
    20
    
    Rat
    20
    
    Rabbit
    20
  - B-Cells
    20
  - Immunization
    20
  - Screening
    20
  - Harvesting Spleen Cells
    21
  - In Vitro Activation
    21
- Cell Preparation on Electrofusion Day
  21
  - Medium
    21
  - Chamber Cleaning
    22
  - Washes and Other Preparatory Needs
    22
  - Cell Preparation Process
    22
- Electrofusion
  23
  - Pre-Fusion Pulse AC
    23
  - Fusion Pulse
    23
  - Post-Fusion Pulse AC
    23
- Cell Handling after Electrofusion
  24
  - Immediate Post-Fusion Care of Cells
    24
  - Materials Needed for Post Electrofusion Culture
    24
    
    Cloning Medium 1
    24
    
    Complete Growth Medium
    24
    
    HAT Stock (50X)
    24
    
    HT Stock (50X)
    24
  - Method, Culture of Cell and Clone Selection
    25
  - Culture of Cells
    25
  - Feeding Cells
    25
  - Cloning Cells
    25
  - Weaning Clones
    25
- Method of Analysis
  25
- Demonstration Experiment to Illustrate Turbulence
  26
- Optimization
  26
- Chamber Cleaning
  26
- Hybrimune Electrofusion Protocol Summary
  27
- Hybrimune Electrofusion Protocol Summary
  28
Cell Electrofusion Tutorial

29
Specifications

44
- Maintenance
  46
- Fuse Replacement
  46
- Figure 22: Fuse Replacement
  46
- Ordering Information
  47
- References
  48
Appendix A: Medarex Data

49
Appendices

49
Appendix B: MEDCOAT 200 Summary Test Results

51
- Appendices
  51

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