cytiva ReadyToProcess WAVE 25 Manuals

cytiva ReadyToProcess WAVE 25 Manuals

Manuals and User Guides for cytiva ReadyToProcess WAVE 25. We have 1 cytiva ReadyToProcess WAVE 25 manual available for free PDF download: User Manual

cytiva ReadyToProcess WAVE 25 User Manual

cytiva ReadyToProcess WAVE 25 User Manual (290 pages)

Brand: cytiva | Category: Laboratory Equipment | Size: 5 MB

Table of Contents

Table of Contents
2
1 Introduction

5
- Important User Information
  6
- About this Manual
  8
- Associated Documentation
  9
- Abbreviations
  10
2 System Description

11
- System Overview
  12
- Readytoprocess WAVE 25 Rocker
  16
- Readytoprocess CBCU
  24
- Readytoprocess Pump 25
  27
- Readytoprocess Pump
  27
- Cellbag Bioreactor
  29
- UNICORN Software Overview
  32
  - General UNICORN Operation
    33
  - UNICORN Help
    34
3 The UNICORN Software

36
- Administration
  37
- System Control
  38
- Methods in UNICORN
  46
  - Method Editor
    47
  - Method Creation
    49
  - Work with Methods
    53
  - Text Instructions
    59
  - Save a Method
    67
  - Scouting
    71
  - Method Queues
    77
- Evaluation in UNICORN
  82
  - Evaluation
    83
  - Open and View Results
    85
  - Run Documentation
    89
  - Generate and Print a Predefined Report Format
    94
  - Create a New Report Format
    96
  - Edit an Existing Report Format
    105
4 System Control Description

107
- Single and Dual Operation Modes
  108
- Temperature Measurement and Control
  109
- Ph and DO Measurement and Control
  110
  - Ph and DO Measurement Principles
    111
  - Ph and DO Control Principles
    113
- Ph Control
  115
  - Ph Control Schemes
    116
  - CO2 Control Mode
    118
  - Acid/Base Control Mode
    120
  - Ph Control Transition Delays
    125
- DO Control
  126
  - DO Control Schemes
    127
  - O2 Control Mode
    128
  - Speed Control Mode
    130
  - O2/Speed Control Scheme
    131
- Media Control
  132
  - Weight Measurement
    133
  - Media Addition
    134
  - Perfusion
    135
  - Deviation Alarm
    139
  - Media Control Inactivation
    140
- Recommended Operating Conditions
  141
- System Verification
  144
5 Operation

149
- Set up the System
  150
  - Select the Tray and Cellbag Bioreactor
    151
  - Attach and Detach Tray
    152
  - Prepare Ph and DO Sensors
    155
  - Attach the Cellbag Bioreactor
    158
  - Prepare the Pump
    160
  - Connect Gas to the System
    165
  - Connect the Filter Heater to the Rocker
    169
- Start and Configure the System
  170
  - Start the System and Log on to UNICORN
    171
  - Connect to the System
    172
  - Configure System Properties
    174
  - Configure System Settings
    176
  - Start a Run
    182
- Prepare for Cultivation
  187
  - Inflate the Cellbag Bioreactor
    188
  - Adjust Pump Parameters
    189
  - Final Checks before Cultivation
    190
  - Add and Equilibrate Culture Medium
    191
  - Prepare the Sensors
    194
- Perform Cultivation
  197
  - Inoculate the Culture
    198
  - Monitor and Control the Run
    199
  - End a Run
    206
6 Maintenance

208
- Calibration
  209
- Pump Calibration
  211
- Cleaning
  214
7 Troubleshooting

215
- Readytoprocess WAVE 25 System
  216
- Readytoprocess WAVE 25 Rocker
  217
- Readytoprocess CBCU
  221
- Readytoprocess Pump 25
  233
- Cellbag Bioreactor
  234
- Software Problems
  236
  - Troubleshooting Method Editor
    237
  - UNICORN System Control
    239
  - Troubleshooting Evaluation
    242
8 Reference Information

243
- System Specifications
  244
- Component Specifications
  245
- Client Computer Specifications
  248
- Chemical Resistance
  250
- Control Settings
  251
  - Rocking Control
    252
  - Heating Control
    255
  - Gas Flow Control
    256
  - CO 2 MIX Control
    258
  - MIX Control
    259
  - Pump Control
    260
  - Ph Measurement
    263
  - Ph Control
    265
  - DO Measurement
    272
  - DO Control
    274
  - Media Control
    278
- Digital and Analog I/O Connections
  280
- Ordering Information
  283
Index

285

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