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Axoporator 800A
User Manuals: Axon Axoporator 800A Electroporator
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Axon Axoporator 800A Electroporator manual available for free PDF download: Theory And Operation
Axon Axoporator 800A Theory And Operation (79 pages)
SINGLE-CELL ELECTROPORATOR
Brand:
Axon
| Category:
Laboratory Equipment
| Size: 8 MB
Table of Contents
Table of Contents
5
Chapter 1 Introduction
9
Chapter 2 Functional Checkout
13
Chapter 3 Installation
17
Standard Configuration
17
Micromanipulator
17
Microscope
18
Connections
18
Optional Configurations
19
Oscilloscope
19
Chapter 4 Reference Section
21
Audio Monitor
21
Headstage
22
High Voltage Precautions
22
Static Precautions
22
Hot Plugging
22
Types
22
Voltage Clamp Circuit
22
Noise
24
Mounting
24
Bath Connection
24
Electroporating While Recording
24
Cleaning
24
Front Panel Controls
25
Front Panel Display
26
Rear Panel Inputs/Outputs
30
Overview of Pulses and Trains
33
Terminology
35
Frequency Equals the Inverse of the Duration between the Start of Two Successive Pulses in the Train
35
Using Axoporator's Pulse Generator
35
Triggering
36
Micropipette Holder
36
Holder Design
36
Holder Use
38
Holder Maintenance
39
Adapters
39
Model Cell
40
Chapter 5 Tutorial
41
Guidelines for Single-Cell Electroporation
41
Micropipettes
41
Selecting the Stimulation Parameters
46
Overview of Protocols for Loading Dissociated Cells in Culture
48
Testing the Setup
51
Chapter 6 Troubleshooting Guide
53
General Rule
53
Symptom: High or Infinite Tip Resistance
53
Symptom: Decrease in Tip Resistance
53
Symptom: Positional Drift
54
Symptom: Contents Leaking from Micropipette Tip
54
Symptom: Unable to Load Cells
54
Symptom: Current or Power Reading Is out of Range
55
Symptom: Grey out of Pulse Frequency or Width Reading
55
Symptom: no R Value Is Reported
55
Chapter 7 Specifications
57
AP-1A Headstage (Voltage Clamp)
57
AP-1A-1MU (Standard)
58
AP-1A-0.1MU (Optional)
58
Micropipette Holder
59
Main Unit
59
Operating Modes
59
Offset Voltage
60
Pulse Types
60
Pulse Generation (Active Mode, Internal)
60
LCD Display
61
Rear Panel
62
Power Requirements (Using the Provided Power Supply)
64
Model Cell
64
Accessories Provided
64
References
65
Technical Assistance
69
Warranty and Repair Service
71
Declaration of Conformity
75
Important Safety Information
77
Index
79
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