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Hoefer DALT
Amersham Biosciences Hoefer DALT Maker Manuals
Manuals and User Guides for Amersham Biosciences Hoefer DALT Maker. We have
1
Amersham Biosciences Hoefer DALT Maker manual available for free PDF download: User Manual
Amersham Biosciences Hoefer DALT User Manual (70 pages)
Brand:
Amersham Biosciences
| Category:
Laboratory Equipment
| Size: 0 MB
Table of Contents
Table of Contents
5
Hoefer DALT System Function and Description
7
DALT System Components
7
Unpacking
11
Important Information/Informations Importantes
12
Specifications
13
Required or Convenient, but Not Supplied
15
Preparing the Gel Caster
16
Casting Homogeneous (Non-Gradient) Gels
18
Preparation for Gradient Gel Casting
20
Configuring the Gradient Divider
20
Calibrating the Peristaltic Pump
21
Casting Gradient Gels
22
Gradient Casting Setup
22
Pouring Gel Solutions for Gradient Gels
23
Applying Overlay to DALT Slab Gels
26
Polymerization
26
Unloading the Gel Caster
27
Filling the Tank with SDS Electrophoresis Buffer
28
Connecting a Refrigerated Circulating Bath
28
Preparing the DALT Tank for Electrophoresis
28
Loading and Running Second Dimension Gels
31
Equilibrating IPG Strips
31
Loading the IPG Strips Onto the DALT Slab Gels
33
Loading Cassettes into the DALT Tank
35
Electrophoresis Conditions in the DALT Tank
36
Unloading the DALT Cassettes
37
Cleaning DALT Cassettes
37
The DALT Blotting Kit
38
Preparing the DALT Tank for Transfer
38
Assemble the Transfer Cassette
39
Loading the Cassettes
41
Final
50
Acryl. Stock
50
M Tris-Cl, Ph
50
10% Aps 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00
50
Water 400 366 333 300 267 233 200 167 133 100
50
10% Sds
51
Gradient Gel Solutions
53
10% Aps
54
10% Temed
54
Glycerol
54
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