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CONTACT INFORMATION:
Peter Owens: 091 494036 (office)
Jennifer Connolly: Jennifer.connolly@nuigalway.ie
REQUIREMENTS FOR EQUIPMENT USAGE:
1. CMI user
2. Completion and signing of Microscope Safety Checklist
3. Certification by Peter Owens or Jennifer Connolly
REVISION LOG
Revision
Date
1.0
12/5/14
1. Purpose
1.1.
This document specifies the work instructions for the CMI Witec Confocal Raman microscope located in
the ground floor Physiology lab, Quadrangle building. If you see an area where more clarification is
needed, if additional information is needed, or if you have suggestions on how to make this guide more
useful in the lab, please contact the CMI.
1.2.
Note that this document is not a detailed instrument manual and does not intend to be one. For
detailed questions or if anything unusual happens with the system, please refer to the manuals
present in the lab, or ask CMI personnel for help.
CMI STANDARD OPERATING PROCEDURE
Confocal Raman Microscope
Physiology Ground floor research lab
CMI document ID: SOP003
Peter.owens@nuigalway.ie
Author
Changes
P. Owens
Initial draft
CMI | SOP003_rev1.0
1
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Summary of Contents for CMi SOP003
- Page 1 1. Purpose 1.1. This document specifies the work instructions for the CMI Witec Confocal Raman microscope located in the ground floor Physiology lab, Quadrangle building. If you see an area where more clarification is needed, if additional information is needed, or if you have suggestions on how to make this guide more useful in the lab, please contact the CMI.
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Page 2: Important Notes
Please contact the CMI staff for more assistance if required. Changes may occur when a new software version or patch is installed. Please contact the CMI staff if you are not sure about new features and functions. - Page 3 CMI staff. Maintaining a clean workspace. Food and drink are NOT allowed! Reporting any and all maintenance issues/concerns to the supervising CMI staff member immediately. Raman overview Raman spectroscopy is a characterization technique that is widely used in scientific field in recent years. It actually utilizes the unique Raman spectra for different components as a spectral “finger print”...
- Page 4 Familiarise yourself with the system folders, located on the shelf above the equipment. Start Up Procedure Book equipment on CMI web booking system. Turn on lasers or individual laser, if only one is to be used. For the 633 laser, turn on the key. For the 785 nm laser, turn on power first and wait until the green led stops flashing.
- Page 5 Both the lasers have to be warmed up for at least 30 mins before acquisition; while warming up alignment of the microscope can be done. Click on the ‘Witec Project Control’ to start the raman software interface Modulate shutter laser intensity 633nm laser 785 nm laser shutter CMI | SOP003_rev1.0...
- Page 6 Set the brightness to 100% in the software (‘Illumination’). Make sure to see some intensity changes in the video image; check it by opening and closing the aperture stop (A) at the microscope. Typically keep this just slightly open, unless for dim samples/dic imaging. CMI | SOP003_rev1.0...
- Page 7 Fine tune the focus with remote observing the image of the field stop again. Then open the field stop to observe the silicon sample. CMI | SOP003_rev1.0...
- Page 8 Record the spectrum for silicon , say 0.5 integration time , verify that the peak intensity is as expected and in the right place ie 520 rel wavenumbers. 8.21 Close the laser shutter and stop the oscilloscope. Alignment of the microscope is now completed. CMI | SOP003_rev1.0...
- Page 9 C-H stretching around 700 and 1000 wavenumbers can be used for mapping. After selecting the large area scan images with better contrast (between cells and nanoparticles), data analysis – background correction, cosmic ray removal, basis analysis – can be performed on large area scan data. CMI | SOP003_rev1.0...
- Page 10 CLEAN UP the workplace, and leave it better than you found it. 11.7 Close the software, it will take several minutes to warm up the CCD before the software fully closes. Log-off the computer. Log out from computer, complete log book. CMI | SOP003_rev1.0...
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Page 11: Appendix A - Certification: Demonstration Of Skills
Demonstrate changing the fibre cable during the measurement session Demonstrate finishing and shutting down system SAFETY Where are cover slips, glass slides discarded? Where are biological samples discarded? Correct use of lasers and proper handling procedures? VALIDATION Certification: Pass: Fail: Trainee Certified by: Date: CMI | SOP003_rev1.0...
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