Table of Contents
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Table of Contents
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Summary of Contents for Fisher Scientific 11350119
- Page 1 Inverted Microscope Instruction Manual Model 11350119...
- Page 2 Introduction Thank you for your purchase of a Fisher Scientific microscope. Your new microscope is a precision instrument carefully checked to assure that it reaches you in good condition. It is designed for ease of operation and years of carefree use. The information in this manual probably far exceeds what you will need to know in order to operate, troubleshoot and maintain your microscope.
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Page 3: Table Of Contents
Fisher Scientific Inverted Microscope Table of Contents Nomenclature Specifications Setting Up the Microscope Assembling the Microscope Input Voltage Installing the Lamp Mounting the Condenser Installing the Objectives Mechanical Stage Mounting the Eyepieces Microscopic Procedure: Interpupillary Distance Adjustment Diopter Adjustment Centering the Condenser... -
Page 4: Nomenclature
Nomenclature 1. Lamp Socket Clamp Screw Knob 8. Objectives 2. Lamp House Cover Clamp Screw 9. Revolving Nosepiece 3. Annular Diaphragm 10. Coaxial Coarse/Fine Focus Knob 4. Condenser Diaphragm Lever 11. Torque Adjusting Ring 5. Condenser Focus Knob 12. Filter Retaining Ring 6. - Page 5 1. Vertical Photo Port 10. Phase Slider 2. Interpupillary Distance Scale 11. Condenser Clamp Holder Screw 3. Diopter Adjustment Ring 12. Condenser Centering Screws 4. Eyepiece 13. Stage Plate 5. Optical Path Selector Lever 14. Fluorescence Filter cassette Mount 6. Filter Slider 15.
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Page 6: Specifications
Specifications • Magnification Ratio: 40X - 200X • Eyepiece: Objective Field Ø22 • Objectives: Magnification N.A. W.D. (mm) Plan Achromat 4X 0.10 23.5 Plan Achromat Phase 10X 0.25 Plan Achromat Phase LWD 20X 0.40 • Condenser: N.A. 0.3 / W.D. 72mm •... -
Page 7: Setting Up The Microscope
Setting Up the Microscope Working environment • The location should be free from dust, moisture, chemical vapors and mechanical vibrations. • Do not place the instrument in a warm and/or humid environment. • Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp or a well- lit bright wall. -
Page 8: Mounting The Condenser
• Replace the stage plate insert. 4. Mechanical Stage • Secure the mechanical stage to the 11350119 plain stage using the two mounting screws located beneath the stage on the right side 5. Mounting the Eyepieces • Remove the dust caps from the eyepiece tubes. -
Page 9: Microscopic Procedure: Interpupillary Distance Adjustment
Microscopic Procedure: Interpupillary Distance Adjustment • Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective. • Adjust the interpupillary distance so that both the right and left field of view become one. • This adjustment will enable the user to observe the specimen with both eyes. 1. -
Page 10: Centering The Lamp
slightly smaller than the eyepiece field of view. • For normal observation, the size of the diaphragm should be just outside the edge of the field of view. 3. Centering the Lamp • Set the Phase annular diaphragm slider in the center position (O). •... -
Page 11: Phase Contrast Microscopy
5. Phase Contrast Microscopy • Phase contrast objectives are labeled Ph. • Fully open the aperture diaphragm. • Bring the 10x (Ph1) objective into optical path. • Position the Phase annular diaphragm slider to 10 -20. Set slider to 40 when using a 40x (Ph3) objective (optional). -
Page 12: Photomicrographic Procedure
Photomicrographic Procedure • The optical path selector lever can be used to set the optical path to either the binocular tube 100:0/0:100 (beam split for fluorescence) or binocular tube/vertical tube 20:80/100:0 (for brightfield or phase). • Before starting photomicrography, check the following: •... -
Page 13: Troubleshooting Table
Troubleshooting Table The troubleshooting table below contains the most frequently encountered problems and their possible causes. Optical and Operating Problems Problem Possible Cause • Lamp not installed properly • Filter slider in intermediate position • Phase slide not in click-stop position •... -
Page 14: Care And Maintenance
Care and Maintenance 1. Lenses and Filters • To clean lens surfaces or filters, first remove dust using compressed air. If dust still persists, use a soft/ clean brush or gauze. • If excessive dirt or grease gets on lens surfaces, a small amount of distilled water or eyeglass lens cleaner can be used on a lens tissue. -
Page 15: Fisher Scientific Warranty
The Fisher Scientific Warranty for cameras is 1 year. Other than set forth above, Fisher Scientific hereby disclaims all warranties, expressed or implied, of fitness for a particular purpose. Defective products covered by the warranty will be repaired free of charge when they are returned, postpaid, to: Fisher Scientific c/o National Optical &... - Page 16 © 2014 Thermo Fisher Scientific Inc. All rights reserved.
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