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Desktop Microscope User Guide
Version 1.1.0
4Deep inwater imaging
6589 Chebucto Road
Halifax, Nova Scotia B3L 1L9
Canada
tel: (902) 444-4449
info@4-deep.com
www.4-deep.com
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Summary of Contents for 4Deep Desktop Microscope

  • Page 1 Desktop Microscope User Guide Version 1.1.0 4Deep inwater imaging 6589 Chebucto Road Halifax, Nova Scotia B3L 1L9 Canada tel: (902) 444-4449 info@4-deep.com www.4-deep.com...
  • Page 2: Table Of Contents

    Contents 1 Introduction 2 General Safety Information 3 What’s in the Box 4 The Microscope and its Components 4.1 Camera ......... 4.2 Sample Stage .
  • Page 3: Introduction

    4Deep. With its new and unique method of data acquisition and analysis, this system is designed to optimize your research of the microscopic world.
  • Page 4: Camera

    Figure 1: Main view of the Desktop microscope 4.1 Camera (a) SSD scale on the opposite side Figure 2: The camera vertical control knob The the digital camera included with the microscope is capable of 15 fps at full (2048x2048) resolution.
  • Page 5: Sample Stage

    4.2 Sample Stage The sample stage has two separate control knobs, one located next to the laser power switch which controls the vertical (z) transla- tion stage shown in Fig. 4, and a second set of vertically aligned knobs on the other side of the microscope which control the x-y transla- tions of the stage, in Fig.
  • Page 6: Microscope Setup

    Plug the 12 VDC camera power cable into the outlet shown in Fig. 3 on page 4. Some models of the Desktop microscope will have a separate 6 VDC power cable for the laser; this is to be connected to the outlet located just below the switch for the laser, shown in Fig. 4 on the previous page.
  • Page 7: Software

    The reconstructed images can be further analyzed, saved, or assembled into the volume re- constructions based on the application. 7 Software The holograms acquired by the Desktop microscope camera need to be properly processed in order to reconstruct the image. 4Deep provides 2 standard software packages, Swordfish, Octopus, and Stingray, which incor- porate our patented fast hologram reconstruction algorithm as well as additional functionality.
  • Page 8: Care And Maintenance

    The distance between the camera sensor and the point source - Source to Screen Distance (SSD) of the Desktop microscope is adjustable. Contact 4Deep for the development of custom configurations for special applications. In order to achieve accurate images, inline holography requires that a reasonable amount of the reference wave (unscattered light) reaches the camera sensor.
  • Page 9: 11 Specifications

    List of Figures Main view of the Desktop microscope ......The camera vertical control knob ......

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