Amersham ÄKTA UPC-900 User Manual
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Amersham ÄKTA UPC-900 User Manual

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Monitor UPC-900
User Manual
18-1125-55

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  • Page 1 Monitor UPC-900 User Manual 18-1125-55...
  • Page 2 ÄKTA monitor UPC-900 ices are sold subject to the terms and conditions of Workstation. sale of the company within the Amersham Biosciences group which supplies them. A copy of these terms and Safety symbols conditions is available on request.

  • Page 4: Table Of Contents

    Cleaning and checking the module ..............22 Cleaning the UV flow cell in-place...............23 Cleaning the UV flow cell off-line................23 Cleaning the conductivity flow cell in-place............24 Cleaning the conductivity flow cell off-line............24 Changing the conductivity flow cell..............24 Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 5 Setup unit number ..............44 B.2.16 Setup display angle ..............44 Setting and using the alarm timer..............45 Service displays ..................45 Menu overview ..................46-47 Technical specifications ..................48 Operating data ..................48 Physical data .....................49 Accessories and Consumables ................51 Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 6 About this manual This manual comprises two parts; a practical part (sections 1 – 5) and a reference part (sections A – D). Sections 1 – 5 contain the necessary information for operating the instrument. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 7 Contents Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 8: Introduction

    Introduction 1 Introduction 1.1 General Monitor UPC-900 is a high precision on-line monitor for the combined measurement of UV absorption, pH and conductivity in liquid chromatography. The UPC-900 features: • Fixed wavelengths of 254, 280 nm (Hg lamp), or 214 nm (Zn lamp).

  • Page 9: Safety

    WARNING! HCl and NaOH are injurious to health. Avoid spillage. WARNING! Hg lamps contain small amounts of mercury and must be handled with care and disposed of according to national or local environmental regulations. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 10: Installation

    0.1 m is required behind and in front of the module. Do not place soft material under the module to ensure that the ventilation inlet below the front is not blocked. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 11: Installing The Optical Unit

    The optical unit can be placed up to 1.5 m from the monitor housing. Holder Optical unit Secure it by tightening the screw in the holder. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 12 The Hg optics with 254 and 280 nm filters and the Zn optics with the 214 nm filter are delivered with filters installed. If other filters are to be used, install the new filters as described in 2.10 Installing optical filters (optional). Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 13: Installing The Conductivity Flow Cell

    Connect the conductivity cell to the socket Conductivity Flow Cell on the rear panel of the module. Connect the tubing with fingertight connectors. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 14 Note: If the electrode is not fully holder inserted, the system will leak and a dead volume will occur in the holder. Connect the pH electrode cable to the socket on the pH Probe rear of the module. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 15: Connecting Electrical Signal Cables

    As a stand-alone module, it is controlled from the front dial and display. In ÄKTA , the monitor is controlled from a PC running FPLC UNICORN version 3.0 or higher via UniNet 1 cables, or manually ™ from the front dial and display. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 16: Connecting To Supply Voltage

    PC and a termination plug. The UniNet 1 link connects, in series, the PC with Pump P-920, Monitor UPC-900 and the Frac-900. The termination plug is connected to the last module in the chain.

  • Page 17: Installing Optical Filters (Optional)

    10 Check that all filters are clean. Insert the filter wheel back into the filter housing. Note: The filter wheel can be placed only in correct position. 11 Reassemble the filter housing with the detector housing by fastening the four screws. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 18: Operation

    Return one menu level, or accept setting level Select sub-menu Return one menu level If a menu has sub levels, the sub menu is displayed by pressing Pressing moves back one menu level. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 19 The cursor moves back one place to the right every two seconds if the dial is not turned. The text or numerical value displayed is accepted by pressing . To cancel, press Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 20: Main Menu Overview

    However, in most cases, a warm-up time of 15 minutes is sufficient. Switching the lamp on/off Switch the lamp on/off by moving the cursor with the dial, and Lamp then pressing OK. (on) on off Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 21: Reading The Uv Absorbance Value

    Note: The user interface of the monitor must be unlocked if connected to a UNICORN control system. Setting the Cond analogue output Current analogue settings are displayed (zero and full scale Set Cond Analog. Out values). Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 22 Set UV Analogue Out values). Allowed full range values are 0.0001, 0.0002, 0.0005, 0.001, 0.002, 0.005, 0.01, 0-02, 0.05, 0.1, 0.2, 0.5, 1.0, 2.0 and 5.0. Zero level is set as a percentage of full scale. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 23: Calibrating Conductivity

    1 pH unit. Calibration can also be performed from UNICORN. In UNICORN, select . Select the pH System Control:System:Calibrate monitor. The calibration procedure can be done with the pH electrode either fitted in or removed from the flow cell. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 24 Turn the dial clockwise to access the buffer 2 calibrating menu. Calibr pH Buffer 2 Rinse the electrode tip with distilled water and then immerse the electrode in the second buffer solution (e.g. pH 4.0 or 9.0) and press Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 25 Switch to the second standard buffer solution and repeat the procedure. For a description of calibration from UNICORN with the electrode fitted in the flow cell, see Chapter 6 in the UNICORN User Manual. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 26: Filtering Noise In The Uv Signal

    5 mm to 2 mm when a lower sensitivity is desired, due to output signal limitation. See section 2.3 Installing the optical unit, sub-section Flow cell installation. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 27: Reading Ph, Temperature And Conductivity Values

    Storage of the conductivity flow cell Overnight: The conductivity cell can be left filled with a buffer. Weekend or long term storage: Flush the conductivity cell with water and fill with 20% ethanol. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 28: Restart After Power Failure

    If the power supply to the module is interrupted, it automatically restarts when power is restored and displays the main operating menu. All set values are retained in the module and the lamp is switched on. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 29: Maintenance

    4 Maintenance WARNING! Always disconnect the power supply before attempting to replace any item on the module during maintenance. CAUTION! Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining and servicing the module. 4.1 Periodic maintenance...

  • Page 30: Cleaning The Uv Flow Cell In-Place

    Maintenance When necessary, replace the lamp according to Section 4.10 Changing the UV lamp, or contact Amersham Biosciences for lamp replacement. Autozero The module internal absorbance value for autozero can be checked to test the consistency of buffers. Select menu...

  • Page 31: Cleaning The Conductivity Flow Cell In-Place

    See section B.2 in Reference information. If the cell constant is not known, it can be determined (see also section B.2 in Reference information). Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 32: Cleaning The Ph Electrode

    Place the electrode tip in the heated KNO solution. Allow the electrode to cool while immersed in the KNO solution before re-testing. If these steps fail to improve the electrode response, replace the electrode. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 33: Changing The Ph Electrode

    Maintenance 4.9 Changing the pH electrode See section 2.4 Installing the pH flow cell and electrode. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 34: Trouble-Shooting

    WARNING! The module must not be opened by the user. It contains high voltage circuits that can deliver a lethal electric shock. 5.2 Faults and actions If the suggested actions do not correct the fault, call Amersham Biosciences. UV measurement No text on the front display 1 Check that the mains cable is connected and the power switch is in ON-position I.
  • Page 35 8 Clean the pH electrode if required, see section 4.8 Cleaning the pH electrode. 9 Compare the response of the pH electrode with that of another pH electrode. If the response differs greatly, the electrode may require cleaning or replacement. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 36 1 Turn the flow cell so the end with the screws is facing the pH flow cell. 2 Recalibrate the conductivity cell. 3 Calibration solution, 1.00 M NaCl, not correctly prepared. Prepare a new calibration solution and recalibrate the conductivity cell. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 37 3.8 and section B.1.4 Setting the Cond analogue output Checking recorder No text on the front display 1 Check that the mains cable is connected and the power switch is in ON-position. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 38: Error Messages

    Trouble-shooting 5.3 Error messages If the suggested actions do not correct the fault, call Amersham Biosciences. Message Action 1 Perform a new start-up. The preceding message may tell 34 Start up failed more about the cause. Retry/Call service 2 If not, call service.
  • Page 39 1 The difference between the zero level and full scale must be 97 WARNING pH scale at least 1 pH unit. Increase the span between zero and full (0-100%)<1pH unit scale settings. See section 3.8. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 40 ERROR number 118 1 Switch off the module. Exc x/y in ab.c 2 Check all connections. 3 Switch on the module. Exc DIV/O in ab.c Exc instr in ab.c Exc address in ab.c Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 41: Reference Information

    Reference information Reference information Description A.1 Module Monitor UPC-900 is an on-line monitor for measurement of UV- absorption, pH (optional in ÄKTA ) and conductivity. The FPLC monitor can work with standard glass pH electrodes with a built in liquid-filled reference electrode and a BNC connector.

  • Page 42: Uv Optcal Unit

    For 214 nm wavelength, a Zn lamp is used. This lamp is larger than the Hg lamp and is therefore mounted in a larger lamp housing. The lamp connectors are keyed to inform the monitor software of which lamp type is connected. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 43: Ph Eletrode

    Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 44: B Menu

    Service information relevant to the module can be checked. Information may not be available in all menus. Select main menu and press Check Select sub menu and press Check Service Mode Check Service Mode Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 45: Setup Menus

    A warning message is shown until confirmed by pressing Warning! This will change temp calib The current adjustment value is displayed as default. Enter the Setup Adjust Temp temperature shown on the thermometer and press (22.3°C) 22.3 Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 46: Setup Show Temperature

    System Control:System:Calibrate CondCalib Note: The conductivity temperature compensation must not be used when adjusting the cell constant. Set the Setup Cond Temp Comp 0 (see section B.2.4). The temperature sensor must be calibrated Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 47 (83.53mS/cm) 86.6 displayed as default. Enter the theoretical conductivity value according to the graph and press The new cell constant is automatically calculated. The range is 1.000- 999.9 mS/cm. Temperature (°C) Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 48: Setup Adjust Cell Constant

    Setup Show Cond status for showing conductivity is displayed. If is shown, (on) current conductivity is displayed in main menus 1 and 2. If shown, no conductivity is displayed in the main menus. Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 49: Setup Ph Temperature Compensation

    Change the setting if desired and press Setup Show pH (on) on off B.2.11 Setup UV averaging filter constant Select sub menu and press Setup UV Setup Temp Cond pH UV Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 50: Setup Lamp Run Time

    1. Change the setting if desired and press Setup Show UV (on) on off B.2.14 Setup language The language used on the display can be changed. Select main menu and press Setup Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 51: Setup Unit Number

    UniNet-bus. It should correspond to the number set in UNICORN for the Monitor UPC-900. The number should be set to 0 if one UPC-900 is used. If more than one UPC-900 monitor is used, they must all have different numbers.

  • Page 52: Setup Display Angle

    B.4 Service displays The module has service displays for use by authorised service Insert Access Code: personnel. If the service display is accidentally Insert Access Code: selected, press to return to the normal operation display. Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 53 Buzzer Test See next page for sub menus Setup Setup Setup Language Set Clock Setup Unit Number Set Timer Setup Display Angle (18:34:52) 00.00.00 Set Alarm 00:32:21 Alarm/Timer 12:30:52 Alarm/Timer off? Buzzer 08:23:34 Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 54 Set Averaging UV sub menus (1.3S) 0.64 Set Averaging (1.3S) Setup Lamp Run Time Setup Lamp Run Time Hg (2000h) Hg (2000h) 3000 Setup Show UV (on) Setup Show UV (on) on off Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 55 Reference information Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 56: Menu Overview

    Max. ±1% or ±5 µS/cm whichever is greater long term Max. ±3% or ±15 µS/cm whichever is greater Noise Max. ±0.5% of full scale calibrated range Response time <3 s (0 - 95% of step) Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 57 Generated backpressure Max. 0.01 MPa at 100 ml/min pH measurement pH range 0 to 14 (spec. valid between 2 and 12) Accuracy temperature compensated ±0.1 pH within +4 to +40 °C not temperature Monitor UPC-900 User Manual 18-1125-55 Edition AE...

  • Page 58: Physical Data

    2000 hours Chemical resistance The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100% ethylacetate, 100% hexane, and 100 % tetrahydrofuran (THF). Monitor UPC-900 User Manual 18-1125-55 Edition AE...
  • Page 61 Short instructions The following short instructions are intended as a guide to users who are fully familiar with the safety precautions and operating instructions described in this manual. These short instructions assume that the module is installed according to the installation instructions.

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