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Burleigh PiezoDrill User Manual

Inertial impact drill
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User's Manual
   
PiezoDrill
Inertial Impact Drill
08572-M-01 (Rev. D)
Copyright ã Burleigh Instruments, Inc. 2002
All Rights Reserved
P
D
is a trademark of Burleigh Instruments, Inc.
IEZO
RILL

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  • Page 1 User’s Manual     PiezoDrill Inertial Impact Drill 08572-M-01 (Rev. D) Copyright ã Burleigh Instruments, Inc. 2002 All Rights Reserved  is a trademark of Burleigh Instruments, Inc. IEZO RILL...

  • Page 3: Table Of Contents

    3. Setup ............................3-1 Filling the Injection Pipette with Fluorinert ................. 3-1 Using Mercury in the Pipette...................... 3-2 Attaching the PiezoDrill to the Microscope and the Micromanipulator ........3-3 Critical Success Factors ......................3-4 PiezoDrill Settings and Control Parameters ................3-4 PiezoDrill Settings When Fluorinert is Used in the Pipette ..........

  • Page 5: Introduction - Mercury Warning

    AND WARRANTIES REGARDING THE USE OF MERCURY IN CONNECTION WITH THE PIEZODRILL.     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 7: Equipment And Materials

    Typical microinjection set up incorporating the PiezoDrill     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 8: Injection Pipettes

    PiezoDrill. The models listed do represent a popular set of choices. We have direct evidence that all of these models work well with the PiezoDrill. Injection Pipettes...
  • Page 9 Inertial Impact Drill User’s Manual 2 Equipment and Materials The PiezoDrill eliminates the need for beveling and drawing a sharp tip on the pipette. These features were required for conventional zona penetration, but the PiezoDrill allows penetration with a flat pipette. Therefore, the optimal pipette is simply broken or cut flat at the precise internal and external diameter required for the application.

  • Page 10: Fluids Used In The Injection Pipette

    We have included one bottle of FC-77 Perfluoro Compound with the PiezoDrill for your initial use. (08572-M-01 Rev D)     Burleigh Instruments, Inc. 2002 The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 11: Holding Pipettes

    20 to 25 µm because: It provides more perivitelline (PV) space between the zona and the oolemma at the other side of the oocyte where the PiezoDrill will penetrate the zona. Since zona vibration settings are higher than the settings for the oolemma, the pipette must not engage the oolemma while vibrating at the zona settings.

  • Page 12: Pipette Holders

    Hollow tube systems may require slightly higher settings on the PiezoDrill amplifier because some of the energy transmitted from the PiezoDrill is lost in the rubber O-ring attachment to the pipette. It is very important that the pipette is held tightly in this connection.

  • Page 13: Solid Pipette Holders

    Optimum amplitude settings are lower with this system because there is no O-ring to absorb energy.     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 14: Microinjector

    Microinjector The Importance of a Good Microinjector A good microinjector is critical to successful use of the PiezoDrill. It is important that the syringe responds precisely to fingertip commands. If pressure inadvertently increases during the procedure, Fluorinert will be pushed out of the end of the pipette.

  • Page 15: Micromanipulators

    This allows for more efficient transfer of vibrations from the PiezoDrill to the pipette tip.     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 17: Setup

    Microbore, 0.38 mm ID, 2.29 mm OD, 0.76 mm wall, Cole- Parmer cat. no. EW-06418-03     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 18: Using Mercury In The Pipette

    Piezodrill, especially for work on mouse oocytes. Burleigh Instruments Inc. does not suggest, approve, endorse or recommend the use of mercury in the PiezoDrill. Mercury should not be used with the PiezoDrill by any individuals or laboratories unwilling to bear the entire risk of exposure to mercury or unable to properly safeguard against mercury exposure.

  • Page 19: Attaching The Piezodrill To The Microscope And The Micromanipulator

    Attaching the PiezoDrill to the Microscope and the Micromanipulator Place the PiezoDrill control unit so that the controls can be easily reached while sitting at the microscope. The PiezoDrill is equipped with foot pedal controls that allow two independent sets of parameters to be readily accessed via independent foot pedals.

  • Page 20: Critical Success Factors

    PiezoDrill Settings When Mercury is Used in the Pipette (08572-M-01 Rev D)     Burleigh Instruments, Inc. 2002 The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.
  • Page 21     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 22: Using The Piezodrill To Break Off The Sperm Tail

    Using the PiezoDrill to Break Off the Sperm Tail To remove the sperm tail with the PiezoDrill, draw the sperm cell into the pipette tail first. With the sperm head half in and half out of the end of the...

  • Page 23: Recommended Procedure For A Successful Injection With Fluorinert In The Pipette

    Figure 8: Pre-injection setup of injection pipette     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.
  • Page 24 Activate pulse 1 on the drill; do not push the injection manipulator at this point – let the PiezoDrill do the work. Increase the negative pressure slightly as the tool drills through the zona (as shown in Figure 10), being careful not to break through the oolemma.
  • Page 25 Proper pressure control and positioning of the sperm or nucleus is the key to success.     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 27: Recommended Procedure For A Successful Injection With Mercury In The Pipette

    PiezoDrill. Please see our mercury warning on page 1-1.     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.

  • Page 29: Troubleshooting Guide

    Oolemma is difficult to See above penetrate.     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.
  • Page 30 (08572-M-01 Rev D)     Burleigh Instruments, Inc. 2002 The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.
  • Page 31 Appendix A Humagen Price List for PiezoDrill Pipettes     Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D) The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh PiezoDrill customers.
  • Page 33 Burleigh Instruments, Inc. 7647 Main Street Fishers Victor, New York 14564-8909 Tel. (585) 924-9355 Fax. (585) 924-9072 Email: info@burleigh.com http://www.burleigh.com PiezoDrill User’s Manual 08572-M-01...

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