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Photometrics Cascade series User Manual

Cascade series
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1K ,
128+,
U S E R M A N U A L
Cascade 650,
512F, 512B,
and 128

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  • Page 1 1K , 128+, U S E R M A N U A L Cascade 650, 512F, 512B, and 128...
  • Page 2 User Manual for Cascade Systems ® Model 650, Model 1K, Model 512F, Model 512B, Model 128+, and Model 128...
  • Page 3 Pentium is a registered trademark of Intel Corporation. QED Capture is a trademark and PVCAM, Cascade, and Photometrics are registered trademarks of Roper Scientific, Inc. Mac and Macintosh are trademarks of Apple Computer, Inc., registered in the U.S. and other countries.

  • Page 4: Limited Warranty

    Photometrics warrants this product against substantial defects in materials and / or workmanship for a period of up to one (1) year after shipment. During this period, Photometrics will repair the product or, at its sole option, repair or replace any defective part without charge to you. You must deliver the entire product to the Photometrics factory or, at our option, to a factory-authorized service center.
  • Page 5 (1) year limited warranty and/or any other warranty, expressed or implied. 3. All warranty service must be made by the Photometrics factory or, at our option, an authorized service center. 4. Before products or parts can be returned for service you must contact the Photometrics factory and receive a return authorization number (RMA).
  • Page 6 When contacting us for technical support or service assistance, please refer to the Photometrics factory of purchase, contact your authorized Photometrics representative or reseller, or visit our technical...

  • Page 7: Declaration Of Conformity

    Declaration of Conformity Roper Scientific, Inc. declares on its sole responsibility that the equipment described in this document is in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC EMC Directive & Amendments 93/68/EEC EMC Directive & Amendments 73/23/EEC Product Safety with Amendments on the application of the laws of Member States relating to Electromagnetic...
  • Page 8 Declaration of Conformity Roper Scientific, Inc. declares on its sole responsibility that the equipment described in this document is in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC EMC Directive & Amendments 93/68/EEC EMC Directive & Amendments 73/23/EEC Product Safety with Amendments on the application of the laws of Member States relating to Electromagnetic...
  • Page 9 Declaration of Conformity Roper Scientific, Inc. declares on its sole responsibility that the equipment described in this document is in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC EMC Directive & Amendments 93/68/EEC EMC Directive & Amendments 73/23/EEC Product Safety with Amendments on the application of the laws of Member States relating to Electromagnetic...

  • Page 10: Table Of Contents

    About This Manual ........................3 Precautions..........................3 Environmental Requirements ....................4 Storage Requirements.......................4 Microscopes, Lenses, and Tripods..................4 Repairs............................4 Cleaning .............................4 Photometrics Customer Service ....................5 Chapter 2. System Installation Introduction ..........................7 Software Compatibility Requirements...................7 Host Computer Requirements ....................7 Multiple Cameras........................8 Software Installation.........................8 Installing the PCI Card......................8 Connecting Your Cascade Camera ..................9...
  • Page 11 PVCAM Error Message Appears..................26 Lengthy Pauses During Imaging ..................26 Chapter 5. Basic Specifications Camera Dimensions (Front) ....................27 Camera Dimensions (Bottom) ....................29 Focal Plane Measurement ......................31 Additional Measurements .....................34 CCD Specifications and Orientation ..................34 Connectors ..........................36 POWER Connector Pinout....................37 I/O Connector Pinout .....................37 Power Supply Specifications and Dimensions..............39 Index ........................

  • Page 12: Chapter 1. Introduction

    The Photometrics ® Cascade ® line of cameras from Photometrics uses an advanced CCD technology called "impact ionization" to achieve charge multiplication gain above the read noise. As a result, the cameras offer unparalleled sensitivity even at high frame rates.

  • Page 13: Technology Introduction

    Technology The principal difference between a charge-multiplying CCD and a traditional CCD is the presence of an extended serial register in the new device (see Figure 1). Introduction Electrons are accelerated from pixel to pixel in the extended portion of the serial register (also referred to as a multiplication register) by applying higher-than-typical CCD clock voltages.

  • Page 14: About This Manual

    PCI Card Power Supply with Power Cord About This The Cascade User Manual is divided into five chapters. It is suggested that you read the entire manual before operating the camera in order to ensure proper Manual use. The chapters that follow this introduction are briefly described below. Note: The information in these chapters applies to the Cascade:650, the Cascade:1K, the Cascade:512F, the Cascade:512B, the Cascade:128+ , and the Cascade:128 models unless otherwise specified.

  • Page 15: Environmental Requirements

    The Cascade camera system contains no user-serviceable parts. Repairs must be done by Photometrics. Should your camera system need repair, contact Photometrics Customer Service. Please save the original packing materials so you can safely ship the camera system to another location or return it for repairs if necessary.

  • Page 16: Photometrics Customer Service

    Photometrics If you have any questions about your camera system, contact Photometrics Customer Service. When you call, please have your Roper Scientific job number Customer Service or equipment serial numbers available. • Tel: 800.874.9789/ 520.889.9933 between 8:00 am and 5:00 pm MST •...
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  • Page 18: Chapter 2. System Installation

    Keep all the original packing materials so you can safely ship the Cascade system to another location or return it for service if necessary. If you have any difficulty with any step of the instructions, call Photometrics Customer Service. Software The Cascade package includes the PVCAM drivers and the QED Capture software program designed for use with your Cascade camera.

  • Page 19: Multiple Cameras

    Windows-based computers. Additional instructions are included for installing a PCI card in your computer and capturing images. The Photometrics CD-ROM contains the following files. • Linux directory — this directory contains the files for installing on a Linux PC.

  • Page 20: Connecting Your Cascade Camera

    Connecting Your The following connectors are located on the back of the Cascade camera: Cascade Camera • DATA connector: 20-pin, high-density connector for data transfer. • POWER connector: 5-pin, LEMO connector for camera power (see POWER Connector Pinout on page 36 for details). •...

  • Page 21: Adjusting The C-Mount Adapter

    Adjusting the The Cascade features an adjustable C-mount adapter that allows you C-Mount Adapter to change the focal depth. Use a .050" hex key to adjust the adapter: loosen the two setscrews, rotate the ring to the desired height (the (2) Setscrews (.050"...

  • Page 22: Chapter 3. Operating Features

    Chapter 3. Operating Features Common Features The features described in this section are identical in the Cascade:650, the Cascade:1K, Cascade:512F, the Cascade:512B, the Cascade:128, and the Cascade:128 cameras. Features that differ between the two models are described separately in the "Cascade:650 Features" and "Cascade:512F/512B/128+/128 Features"...

  • Page 23: Figure 3. Required Settings For Non-Overlap Mode Operation

    In PVCAM compatible software, this sequence is programmed by simply setting: • clocking mode to “Frame transfer” and • clearing mode to “Pre-exposure” with clear count of “1”(or more). Figure 3 shows these settings on a software dialogue box. Note: Since the software you are using may show the settings differently, you should refer to the software documentation for accurate information.

  • Page 24: Figure 5. Required Settings For Overlap Mode Operation

    Referring to Figure 4, it can be seen that exposure and readout are carried out in non-overlapped fashion. As a result, each frame in the sequence is precisely exposed for the time specified (i.e., 10 ms). Overlap Mode Overlap mode is extremely useful in applications requiring continuous imaging (Simultaneous Exposure- (100% duty cycle).
  • Page 25 In Overlap mode when exposure time < readout time, the total time (T ) taken to capture N frames is given by: × N) + t = (t Where = Total time taken to capture a sequence of N frames = readout time for one frame N = total number of frames in a sequence = exposure time...

  • Page 26: Triggered Operation

    Triggered Operation Cascade:650, Cascade:1K, Cascade:128+, Cascade 128, Cascade:512F, and Cascade:512B cameras offer several methods of integration with external trigger sources, such as delay generators or laser pre-triggers. Each camera has a 26-pin, high-density I/O connector (pinout functions are described on page 37) on the back for trigger-in/out and various TTL input and output operations.

  • Page 27: Aimo / Non-Aimo

    Ignored Trigger Exposure Readout Figure 10. Strobe Mode Timing Diagram Bulb Mode Bulb mode only operates in Non-Overlap mode. In Bulb mode, exposure time for each frame is determined by the trigger pulse width. Exposure time entered into the software is ignored in this mode (see Figure 11). If a trigger arrives during the readout of the previous frame, it is ignored.

  • Page 28: Cascade:650/1K Features

    For more information, refer to the "On-Chip Multiplication Gain" technical note. This technical note can be accessed by going to the Photometrics web site at www.photomet.com, clicking on "Library", clicking on "Technical Notes", and selecting the title from the list of notes.

  • Page 29: Readout Speed

    Readout Speed Cascade:650 is implemented with 10 MHz and 5 MHz digitization speeds (10 million (typical) and 5 million pixels per second, respectively). At 10 MHz, it gives approximately 25 full frames per second (fps), whereas 5 MHz operation offers better image quality. With either digitizer, increased frame rate can be achieved by choosing a sub-region.

  • Page 30: Cascade:512F/ 512B/128+/128 Features

    Cascade:512F/ A 12-bit DAC is provided to control the multiplication gain of the Cascade: 512F, Cascade:512B, Cascade:128+, and Cascade:128 in 4096 steps. A DAC setting of 512B/128+/128 zero (0) refers to a no-gain state where the camera behaves like a standard high Features speed CCD (with rather high read noise).

  • Page 31: Figure 15. Software Selection Of Readout Port

    Note: The Cascade:128+ and Cascade:128 are only available with a multiplication port. The Cascade:512F and the Cascade:512B are also available in "multiplication port only" configuration. Please check the configuration for your system. If your camera is configured with two readout amplifiers (ports), the software automatically allows port selection.

  • Page 32: Conversion Gain

    2. Traditional or Non-Multiplication amplifier (Port #2): When the camera is using this amplifier, electrons (signal) generated in pixels are clocked through the standard serial register. The amplifier is designed to take advantage of the dynamic range of the CCD and is most useful when the frame rate is not critical.

  • Page 33: Binning

    A slower readout speed can be used when better noise performance is needed at the expense of frame rate. On the other hand, increased frame readout rate can be achieved by one or more of the following • higher readout speed, •...

  • Page 34: Summary

    Summary Table 5, below, summarizes typical Cascade:512F and 512B settings for two types of applications and is provided as a setup aid. Actual settings will vary based on the exact nature of your experiment design. Readout Conversion Amplifier Readout Multiplication Gain Readout Application...
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  • Page 36: Chapter 4. Troubleshooting

    2. Turn your computer back on. If your system boots normally, there is probably an interrupt conflict between a previously installed expansion card and the PCI card that you are installing. 3. If you need assistance resolving the interrupt conflict, contact Photometrics Customer Service. New Hardware...

  • Page 37: Camera Running Too Warm

    Too Warm inch of space has been left around the external cooling fins for airflow), switch off the camera immediately and contact Photometrics Customer Service. Unlike other Cascade models, the Cascade:128 features a fanless design. Therefore, the cooling performance afforded by the Cascade:128 is highly sensitive to the camera’s orientation and environment.

  • Page 38: Chapter 5. Basic Specifications

    Chapter 5. Basic Specifications Camera Dimensions (Front) Cascade:650 and 1K Cameras - Front View 4.50 in (11.43 cm) (2) Holes .15 in (.38 cm) dia x .15 in (.38 cm) deep 1.25 in (3.18 cm) apart 4.00 in (10.16 cm) 4.30 in (2) Setscrews (10.92 cm)
  • Page 39 Cascade:128 Camera - Front View Cascade User Manual...

  • Page 40: Camera Dimensions (Bottom)

    Camera Dimensions (Bottom) 2.90 in (7.37 cm) 7.01 in (17.8 cm) 7.19 in (18.26 cm) Cascade:650 Camera: Bottom View Cascade:1K Camera: Bottom View Chapter 5. Basic Specifications...
  • Page 41 .09 in (.23 cm) 2.87 in (7.29 cm) 6.98 in (17.73 cm) 7.17 in (18.21 cm) 1/4-20 UNC-2B thread .30 in (.76 cm) max deep Cascade:512F, 512B, and 128+ Cameras: Bottom View Cascade:128 Camera: Bottom View Cascade User Manual...

  • Page 42: Focal Plane Measurement

    Focal Plane Measurement .66 in (16.8 mm) FOCAL PLANE Cascade:650 Camera: Focal Plane Cascade:1K Camera: Focal Plane Chapter 5. Basic Specifications...
  • Page 43 FOCAL PLANE .63 in (16.0 mm) MECHANICAL Cascade:512F and 512B Cameras: Focal Plane .58 in (14.7 mm) FOCAL PLANE .63 in (16.0 mm) MECHANICAL Cascade:128+ Camera: Focal Plane Cascade User Manual...
  • Page 44 .080 in (2.0 mm) Adjustment FOCAL PLANE .65 in (16.5 mm) Mechanical .70 in (17.8 mm) Optical Cascade:128 Camera: Focal Plane Chapter 5. Basic Specifications...

  • Page 45: Additional Measurements

    Additional Camera weight: Measurements Cascade:650, 1K, 512F, 512B, and 128+ 6.5 lb. (2.9 kg) Cascade:128 5.9 lb. (2.6 kg) Specifications and Orientation Cascade:650 Cascade:1K Cascade:512F/ Cascade:128+ Cascade:128 Cascade:512B CCD: TI TC253 TI TC285SPD E2V CCD87F E2V CCD60 E2V CCD60 E2V CCD97B Image Type: Monochrome Monochrome...
  • Page 46 Cascade:1K CCD Orientation Cascade:512F/512B CCD Orientation Cascade:128+ CCD Orientation Chapter 5. Basic Specifications...

  • Page 47: Connectors

    x = 128 (0,0) y = 128 Cascade:128 CCD Orientation Connectors Cascade Rear Panels (Cascade:128 on right) The following connectors are located on the back of the camera. DATA connector: 20-pin, high-density connector for data transfer and power. POWER connector: 5-pin, LEMO connector for camera power. I/O connector: DB26, high-density connector for input/output control signals.

  • Page 48: Power Connector Pinout

    An I/O cable (Part #: 37-107-002) to access Trigger Input (Pin 1), Trigger Invert Input (Pin 2), Frame Readout (Pin 7), Camera exposing output (Pin 8), and Shutter Output (Pin 23) is available from Photometrics. Trigger Input: This input is internally tied high through a 4.7kΩ...
  • Page 49 GND: System digital ground. Any external circuitry intended to interface with the trigger control signals must reference this ground connection. Port 4 DAC 1: 8-bit programmable output (0-5 V) Port 5 DAC 2: 8-bit programmable output (0-5 V) GND: System digital ground. Any external circuitry intended to interface with the trigger control signals must reference this ground connection.

  • Page 50: Power Supply Specifications And Dimensions

    Power Supply Voltage Input: 100-240 V~ @ 47-63 Hz Specifications Current Input: 3.0 A Voltage Output: +12 Vdc Maximum Power Output: 96 W Dimensions Power Supply Weight: 2 lb (0.9 kg) Note: CE certification applies to the Cascade only when the camera system is operated with a CE-approved power supply.
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  • Page 52: Index

    Index Cascade:650 binning, 18 Application examples CCD orientation, 34 Cascade:512F and 512B, 22 conversion gain, 17 Bias, 11 declaration of conformity, vi Binning multiplication gain, 17 Cascade:128, 22 readout speed, 18 Cascade:128+, 22 software compatibility, 7 Cascade:1K, 18 Cascade:512F and 512B, 22 Cascade:128+ orientation, 35 Cascade:650, 18 Cascade:1K orientation, 35...
  • Page 53 Digitization speeds (cont.) Cascade:512F and 512B, 21 Readout modes Cascade:650, 18 non-overlap, 11 Dual digitizers overlap, 13 Cascade:512F and 512B, 21 Readout ports, 19 Cascade:650, 18 Readout speed Dual readout ports, 19 Cascade:128, 21 Focal plane, 31 Cascade:128+, 21 Host computer requirements, 7 Cascade:1K, 18 I/O interface Cascade:512F and 512B, 21...
  • Page 54 49.89.660.779.50 tel: 81.43.274.8022 email: mailto@roperscientific.com fax: 33.160.86.07.09 email: mail@roperscientific.de fax: 81.43.274.8023 email: princeton.instruments@wanadoo.fr email: sales@roper.co.jp Photometrics Princeton Instruments 3440 East Britannia Drive 3660 Quakerbridge Road Tucson, Arizona 85706 Trenton, New Jersey 08619 tel: 800.874.9789 or 520.889.9933 tel: 800.874.9789 or 609.587.9797 fax: 520.295.0299...

This manual is also suitable for:

Cascade 650Cascade 1kCascade 512fCascade 512bCascade 128+Cascade 128

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