Kubota 4200 Instruction And Service Manual
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Kubota 4200 Instruction And Service Manual

Kubota 4200 Instruction And Service Manual

Table－top centrifuge
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NOTE
■The products being indicated in this Instruction Manual are designed for opera-
tors with expert knowledge and are intended only to be used by such qualified
operators observing the indicated precautions for respective purposes. For per-
sons lacking necessary expert knowledge, these products may be difficult to use
properly and may even pose a danger to use. When the aforesaid persons lack-
ing the necessary expert knowledge are using these products, do so under ap-
propriate supervision and guidance of a qualified operator possessing the neces-
sary expert knowledge.
■Do not distribute this manual within the U.S.A., Mexico, Canada and Australia as
the products advertised in the manual shall not be distributed in these countries.
For information added or modified after February 2011, please contact your local dealer.
29-9 HONGO 3-CHOME，BUNKYO-KU，TOKYO 113-0033，JAPAN
TABLE－TOP CENTRIFUGE
INSTRUCTION
AND
SERVICE MANUAL
KUBOTA CORPORATION
January 2011, Printed in Japan
［86 Pages］
M10420108

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  • Page 1 ■Do not distribute this manual within the U.S.A., Mexico, Canada and Australia as the products advertised in the manual shall not be distributed in these countries. For information added or modified after February 2011, please contact your local dealer. KUBOTA CORPORATION 29-9 HONGO 3-CHOME，BUNKYO-KU，TOKYO 113-0033，JAPAN ［86 Pages］...

  • Page 3: Warranty

    Use of Model 4000 / 4200 Model 4000 and 4200 can be used for preprocessing in in-vitro analysis, which breaks down blood or urine samples containing plasmas or cells. Accordingly, it is not designed to connect directly to a patient's body.

  • Page 5: Safety Instructions

    Safety Instructions The centrifuge and the manual indicate important information in order to ensure safe operation of the centrifuge and to prevent physical injuries and property damages. Be sure to understand the meanings of the following indications and follow the instructions.

  • Page 7: Table Of Contents

    Table of Contents page page ３ － ５ ．Setting the acceleration ･ deceleration Safety Instructions ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ Ⅰ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 3-10 General Notes ・...
  • Page 8 page page ⑥ d.5 Section 5. Troubleshooting Setting the timer ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 3-30 ５－１ ．View port flashing yellow (caution display) ⑦ d.6 ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 5-1 Grease application message ・...

  • Page 9: General Notes

    General Notes Be sure to follow them. Since large electrical and mechanical energies are present on the centrifuge and rotor, reasonable care is required for their handling. Otherwise failure may occur resulting in property damages or fatal physical injuries. In order to prevent them from happening,be sure to follow the instruction given below. WARNING （１）Maximum load （５）While the centrifuge is in operation...
  • Page 10 WARNING （１０） Lifetime and usable number of cycles 3. Water pipes of rotors City water piping may not work properly as a ground as it may Use of rotors beyond the lifetime consist of chloroethylene pip- and usable number of cycles may ings.
  • Page 11 CAUTION （２）Toxic or radioactive substances etc. （７）Balance of sample When centrifuging of substan- Keep the load (of the sample, ces contaminated with patho- bucket, etc.) balanced. genic bacteria, or toxic or radio- If an appropriate balance is not active substances, always use provided, unexpected accidents containers that are pathogenic can result from a damaged rotor...

  • Page 12: Standard Accessories

    Standard Accessories １． Instruction manual・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・1 Copy manual ２． Power cable ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・1 Pc. Option Table for Centrifuge Fixing bracket for...

  • Page 13: Usable Rotor

    （２）The rotors that can mount as of January 2011 are as follows: This information is subject to addition or change. For information after February 2011, please contact your local dealer. Model Fixed Angle Rotor Swinging Bucket Rotor Plate Rotor 4000 AT-508C ST-480M ST-504M PT-89M RA-2724M ST-722M...

  • Page 14: Lifetime And Usable Number Of Cycles Of Rotors

    Lifetime and usable number of cycles of rotors WARNING Use of rotors beyond the lifetime and usable number of cycles may lead to breakage of the rotor. If the rotor is used continuously even after the lifetime and usable number of cycles of rotors has expired, should the rotor get damaged, the main unit of the centrifuge suddenly may start to rotate;...

  • Page 15: Number Of Cycles Allowed For Autoclaving Of Rotor

    Number of cycles allowed for autoclaving of rotor WARNING Stop the use of the rotor immediately when it is used beyond the number of cycles allowed for autoclaving. Otherwise, the rotor may deteriorate by the heat generated by autoclaving, resulting in deformation or destruction. Should the rotor get damaged, the main unit of the centrifuge suddenly may start to rotate;...
  • Page 16 Temperature and minutes of Usable number Rotor of cycles autoclave Rotor york Bucket 121 ℃ , 20 minutes 50 cycles ST-2504MS Sealing cap Packing of sealing cap Tube rack Rotor york 126 ℃ , 10 - 15 minutes 100 cycles Bucket (ST-410M) 115 - 118 ℃...

  • Page 17: Component Name And Explanation

    Component Name and Explanation Section 1 Appearance 1 － 1． Running condition lamp Refer to page 3-20. Hook Viewport Refer to page 4-8. Air intake port Drive shaft Chamber as springs Refer to page 3-1, 4-9. “POWER"switch Front cover Refer to page 5-8. Control panel １－１...

  • Page 18: １ － ２ ．Control Panel

    5 seconds. "SPEED" display (Speed / RCF ) MEMORY selection keys Model 4200 : × 10 These keys allow the selection of 3-11 Model 4000 : × 1 saved programs. Pressing the key will light the lamp. "SPEED" setting（△▽） key １－２...

  • Page 19: Installation And Power Supply

    Installation and Power Supply Section 2 Unpacking ２－１． When the centrifuge is taken out of a corrugated carton box, check the followings. （１） Upon receiving the centrifuge, examine it for any visible damage caused during transportation. If any is found, contact the dealer immediately. （２）Confirm that all the accessories listed in [Standard Accessories] are included with the Refer to page Ⅳ...

  • Page 20: ２－４ ．Power Requirement

    Section 2 Installation and Power Supply Power Requirement ２－４. WARNING Prepare a power supply that meets the following conditions. 1. The power supply voltage must be the same as that indicated on the nameplate of the centrifuge and the voltage variation must be within the range given in Table 2-1.

  • Page 21: ２－５ . Grounding

    Section 2 Installation and Power Supply Grounding ２－５ . Inlet NOTE When using the centrifuge for the first time, plug the power cable on the centrifuge into the inlet on the back of the centrifuge. Power plug 　 WARNING Ensure that the ground wire is connected to the grounding terminal. WARNING Do not connect the ground cable to the following places: 1.

  • Page 23: Section 3. Operation

    Operation Section 3 Cautions of Operation ３－１． When using this centrifuge, observe the contents of the Section "General Notes" being described in the front part of this document and the precautions given in respective sections. Operation 　 ３－２． Operation 1. Turn on the "POWER" switch． ．...
  • Page 24 Section 3 Operation Operation 6. Push lightly the center of the front of the lid, and close the lid. Close the lid firmly locked, "Running condition lamp" and "OPEN" lamp care turned off. Operation 6 NOTE If the lid is not closed properly the centrifuge is not able to start.
  • Page 25 Section 3 Operation Operation 11. When the rotor stops, "STOP" lamp remains lit, sound that notify the end of the operation, the Running condition lamp flashes, and the lid automatically opens. SPEED TIME × × MEMORY Ｓ Ｔ Ｏ Ｐ ACCEL STOP OPEN...

  • Page 26: ３－３． Setting The Speed

    ３－３． ［１］ Setting the speed by the rpm ［Ex.］The process mentioned below shows the case that ST-722M rotor is moun- ted. (Model 4000) （１）Mount the rotor on the drive shaft. （２）Press the "NORMAL" key. The "SPEED" display indicates the current setting.
  • Page 27 Section 3 Operation Model 4000 and 4200 indication on the "SPEED" display ① Model 4000 1 rpm intervals. SPEED × g ［Ex.］3,500 rpm ② Model 4200 10 rpm intervals. SPEED × g ×10 ［Ex.］3,500 rpm ［－－－］ is indicated. NOTE Before operation or while the lid is open, the centrifugal force will not be displayed even when the display mode is switched from speed to centrifugal force.

  • Page 28: ２］ Setting The Speed By The Centrifugal

    Section 3 Operation ［２］Setting the speed by the centrifugal force ( × g) （１）Mount the rotor on the drive shaft. （２）Press the "NORMAL" key. The "SPEED" display indicates the current setting. Ｎ SPEED × g （３）Check if the〈× g〉lamp turned on. ①...
  • Page 29 Section 3 Operation Model 4000 and 4200 indication on the "SPEED" display ① Model 4000 １× g intervals. SPEED × g ［Ex.］1,500 ×ｇ ② Model 4200 10 × g intervals. SPEED × g ×10 ［Ex.］1,500 ×ｇ ［－－－］ is indicated. NOTE Before operation or while the lid is open, the speed will not be displayed even when the display mode is switched from centrifugal force to speed.

  • Page 30: ３－４． Setting The Timer

    Section 3 Operation Setting the Timer ３－４． ● As for the setting method of the timer, there are the following 2 kinds. ［1］Set the timer to minutes or seconds. ［2］Set the timer to the desired combination of minutes and seconds. ●...
  • Page 31 Section 3 Operation ［2］Set the timer to the desired combination of minutes and seconds. （１） Check that a period "." is displayed to the right of the "TIME" display. If a period is not displayed, refer to page 3-30 [d.5:Setting the timer] and change the setting. TIME Period "."...

  • Page 32: ３ － ５ ．Setting The Acceleration ･ Deceleration

    Section 3 Operation Setting the acceleration ･ deceleration ３－５． When blowing-up of samples is concerned, adjust the acceleration and the deceleration setting by pressing the "ACCEL/DECEL" key. RAPID acceleration SLOW acceleration ［Ex.］ SPEED １ ５ ０ ０ ｒ ｐ ｍ TIME Refer to page 3-26.

  • Page 33: ３ －６ ．Saving The Memory

    Section 3 Operation Saving the Memory ３ －６ ． Saving the setting value enables operation with the same setting value repeatedly. ［１］ Saving the Memory （ １） Set the respective parameters. Refer to the following page for the setting method. ［...

  • Page 34: ３ － ７ ．Flashing Operation

    Section 3 Operation Flashing operation ３ －７ ． The flashing operation continues as long as the "FLASHING" key is kept depressed. The flashing operation decelerates and stops when the "FLASHING" key is released. Operation 1. At first, execute ordinary Operation 1 - 6 in the ［ 3-2.Operation ］ . ...

  • Page 35: ３ －８ ．Memory Flashing Operation

    Section 3 Operation Memory Flashing operation ３－８． The last ordinary flashing operation is memorized automatically. When the "FLA- SHING" key is pressed shortly (within a second), the memory is called out and the equipment will make spinning down for the same time as the last operation. It is not necessary to keep depressing of the flashing key.

  • Page 36: ３ － ９ ．Setting The Optional Function

    Section 3 Operation Setting the optional function ３－９． This product allows setting the following displays and buzzer sounds for check. （１）Pressing the "TIME" key while holding down the "STOP" key indicates the version number of control software. "TIME" key "TIME" setting（△▽）key TIME Ｓ...
  • Page 37 Section 3 Operation "SPEED" "TIME" Turn Function Outline Page display display [Ex.] ST-722M ID ７ Rotation Radius Setting the desired rotation radius. 3-24 r.rAd [Ex.] 0 rpm "Natural" Setting the natural deceleration ８ 3-25 deceleration starting speed. [Ex.] 1,000 rpm Setting the speed of Na up to which "SUPER SLOW"...

  • Page 38: １］Control Of Software Version

    Section 3 Operation ［１］Control of Software Version To check the software version number for maintenance purposes, follow the method below: Software version number shall be informed when you have any inquiry. （１）Pressing the "TIME" key while holding down the "STOP" key. （２）...

  • Page 39: Of The Rotor

    Section 3 Operation Press the "TIME" setting （△▽）key to display the ID number of the rotor on the "TIME" （３） display. SPEED TIME × g ［Ex.］ ST-722M rotor ID No.22 Rotor number table Rotor Rotor Rotor number number number （ ST-410M ） ST-2504MS AT-2730M ST-480M...

  • Page 40: ３］Setting The Sound That Notify The

    Section 3 Operation ［３］Setting the Sound that notify the end of the operation The "Sound that notify the end of the operation" can be selected from the 5 kinds ＋ Sound none. Use this function to identify each "Sound that informs the end of the operation" when you use more than one centrifuges.

  • Page 41: ４］Setting The Volume For The Buzzer

    Section 3 Operation ［４］ Setting the Volume for the buzzer Three volume levels (mute, low and high) can be selected the "Sound that notify the end of the operation" and "key entry buzzer." Factory default : ［ High(2) ］ （ １） Pressing the "TIME" key while holding down the "STOP" key . The software version number is displayed.

  • Page 42: ５］Setting The Running Condition Lamp

    Section 3 Operation ［５］Setting the Running condition lamp ● The lamp color of "Running condition lamp" of the lid can be selected from "Seven colors shown as follows" by "MEMORY"1～5Key and "NORMAL" Key. ＊ Caution is indicated by the lamp rapidly flashing "yellow." Running condition lamp Alarm is indicated by the lamp rapidly flashing "red."...
  • Page 43 Section 3 Operation （３）Press the "MEMORY" or "NORMAL" key, which you want to choose the color of the lamp. The lamp of "MEMORY" or "NORMAL" key indicates the current setting of the lamp color number The lamp flashes or lights. ［Ex.］It lights by the color to which selected Key and Running condition 　　　lamp are memorized.

  • Page 44: ６］Setting The Delayed Operation Start-Time

    Section 3 Operation ［６］Setting the Delayed operation start-time This function is helpful for biochemical tests and serological tests when the start of operation is required to be delayed for the previously specified time. When centrifugal separation is conducted for the fresh blood taken out, processing will wait until that the erythrocytes coagulate and then will start automatically.
  • Page 45 Section 3 Operation How to check the delayed operation start-time that has been saved ① Implement the operations（１）and（２） given in page 3-22. ② Pressing "MEMORY" key where the delayed operation start time is set, and choose the lamp number you want to recall. The "SPEED"...

  • Page 46: ７］Setting The Rotation Radius

    Section 3 Operation ［７］Setting the Rotation Radius To obtain more accurate centrifugal force, adjust the rotation radius to that of the rotor buckets. Factory default : Initial value of each rotor Since the radius of gyration is different depending upon each of the rotor and each of the bucket, a deviation will occur in the indications of the centrifugal force.

  • Page 47: ８］Setting The "Natural" Deceleration

    Section 3 Operation ［８］Setting the "Natural" deceleration You can set the natural deceleration starting speed Nd by the following method. Factory default， it is set as 0 rpm. The Nd value can be set up to the maximum speed of the rotor that is being used. （１）Pressing the "TIME"...

  • Page 48: ９］Setting The "Super Slow" Acceleration

    Section 3 Operation ［９］Setting the "SUPER SLOW" acceleration You can set the speed of Na up to which the "SUPER SLOW" acceleration is applied by the following method. Factory default， it is set as 1,000 rpm. The Na value can be set up to the maximum speed of the rotor that is being used. （１）Pressing the "TIME"...
  • Page 49 Section 3 Operation ［１ ０］Setting the other Function The following 7 items are possible setting with ON/OFF. Other function lists "TIME" Function Default Page display Buzzer sound of the "START" key. 3-28 Buzzer sound of the "STOP" key 3-28 Operation time after resumption 3-29 Reminder alarm 3-29...

  • Page 50: Setting The "Start" Buzzer Sound

    Section 3 Operation ④ Pressing the "SPEED" setting （△▽） key to change ON or OFF. SPEED TIME × g SPEED × g ⑤ After setting any one of the above, please return the display to the previous indication of Speed and Time. To do above, press the "TIME"...

  • Page 51: Operation Time After Resumption

    Section 3 Operation ③ d.2 ［Operation time after resumption］ When stopping the operation midway and when resuming the operation after that, it is possible to make the setting for resuming the operation from the beginning of the preset time (ON) or to make the setting for resuming the operation for the remaining time (OFF).
  • Page 52 Section 3 Operation ⑥ d.5 ［Setting the timer］ The setting method of the timer is able to select [Set the timer to minutes or seconds.(OFF)] or [Set the timer to the desired combination of minutes and seconds.(ON)]. Refer to page 3-8 [3-4.Setting the timer] Its default setting is OFF.
  • Page 53 Section 3 Operation Calculating Centrifugal Force ３－１ ０. You can readily calculate the centrifugal force by assigning the Rotation Radius and speed in the following equation: Please refer to the Nomograph for Centrifugal Force Calculation at the last page of this manual.
  • Page 55 Service Section 4 Daily Inspection ４－１． WARNING If any abnormality is found during routine daily inspections, discontinue centrifuge operation, use the "POWER" switch to turn the machine OFF, disconnect the power cable, attach "DO NOT USE" labels to the rotor and the outside of the centrifuge, and contact your nearest dealer to request a centrifuge inspection before resuming operation of the equipment.

  • Page 56: Monthly Inspections

    Section 4 Service Monthly Inspections ４－２． ［１］Inspect the rotor WARNING Perform a monthly careful inspection of the rotor appearance. Check for any deformation or damage, including the interior and bottom of the holes. If any abnormality whatsoever is found, there may be significant damage or corrosion of the rotor that could lead to serious damage or accidents involving physical injury.
  • Page 57 Section 4 Service Cleaning and Sterilization ４－４． WARNING Consult with your nearest dealer prior to attempting any cleaning procedure for the rotor, buckets, or tube rack that is not specifically recommended in this manual. Certain cleaning methods or cleaning agents may cause corrosion and then breakage leading to serious damage or accidents involving physical injury.
  • Page 58 Section 4 Service ［２］ Cleaning the rotor, buckets and tube rack CAUTION Do not use detergents exceeding a pH range of 5 - 8, or chlorinated detergents normally used for washing. Corrosion may damage the rotor, bucket, or tube rack, resulting in damage to the centrifuge that may lead to serious additional damage or accidents involving physical injury.
  • Page 59 Section 4 Service ［４］ Clean and disinfect the bottom of the tube accommodation hole Wet a cleaning swab (preparation of which is Tube accommodation hole described below) with warm water and remove any stains or residue adhering to the bottom of the hole. Cleaning swab Rotor If the part must be disinfected, use rubbing alcohol,...
  • Page 60 Section 4 Service Greasing ４－５． CAUTION Be careful to maintain lubrication by ensuring that a proper amount of grease is present in all bucket grooves (section "A" in the schematic diagram below). A lack of grease may allow violent vibration to occur, resulting in damage to the centrifuge.
  • Page 61 Section 4 Service Inspection of Circuit Protector ４－６． The circuit protector is automatically activated when the electric circuit failure or overcurrent occurs. In such a case, the white portion of the circuit protector, located on the rear side of the centrifuge, will stick out.
  • Page 62 Section 4 Service Using the Viewport ４－７ ． A Viewport is provided at the top of the lid. Through the Viewport, the actual rate of rotation can be measured, using a photoelectric tachometer. （ １） When using a photoelectric tachometer, place a piece of reflective tape 30 - 50 mm away from the center of the rotor.
  • Page 63 Section 4 Service Spare Parts Supply ４－８. NOTE Spare parts (parts necessary to the maintenance of equipment functions) will be available for ten (10) years after production of the particular product is discontinued (except spare parts which we are unable to procure). We therefore beg your indulgence and recommend that you be prepared for some situations in which certain repairs are impossible due to a lack of spare parts.
  • Page 64 Section 4 Service ［１］ Required information when submitting an inquiry Required information NOTE １． Model 4000 / 4200 ２． Serial number 　 ［Ex.］H10015 See the product label on the left side panel of centrifuge. ３． Software version Refer to Section "Control of Software Version" on page number 3-16.
  • Page 65 Section 4 Service Product Preparation When Returning ４－１ １. Units for Repair or for Other Reasons ［１］Remove any contaminants from units or items that will be returned for servicing Prior to sending back our products (including accessories) for repair or for other reasons, be sure to remove any and all contaminants.

  • Page 66: Contaminant Elimination Certificate

    Please make a copy of this page and fill out the relevant details. Contaminant Elimination Certificate Date :　　　, 　　 , 　　 Name: 　 　　　　　　　　　　 Company / Institute: 　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　 Department: 　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　 　 　　　　　　　　　　 Tel:　　　　　　　　　　　　　　　 Fax: 　　　　　　　　　　　　　　　　　　　 　　　　　　　 Address: We hereby certify that contaminant elimination for this equipment has been performed as follows: 　　　　　　　　　　　...
  • Page 67 Model 4000 : 10-6,300 rpm, in 10 rpm steps Model 4200 : 10-16,300 rpm, in 10 rpm steps Digital display, Model 4000 : 10-5,350 × g, in 10 × g steps RCF setting Model 4200 : 10-23,470 × g, in 10 × g steps Digital display, Model 4000 : 0-5,900 ×...
  • Page 69 Dispose of the centrifuge unit, Section 7 rotor, and accessories Dispose of the centrifuge unit, rotor, and accessories according to local practices for the disposal of industrial waste. If you encounter problems concerning proper disposal, contact your nearest dealer. If the centrifuge unit, rotor, or accessories to be discarded are contaminated with radioactive, explosive, toxic or infectious substances that may be harmful to people's health, you must notify the waste handlers concerning the details of such contaminants.
  • Page 71 Model 4200 Motor 017-0023 TG-205B-GL-83-F307, 24 V Geared motor 100 Ω 120W, Model 4200 112-0017 Enamel resistor 014-0244 VFNC3S-1004P, Model 4000 For 100-115 V Inverter 014-0245 VFNC3S-2004PL, Model 4000 For 200-240V Inverter 014-0146 VFS11-2007PM-AN, Model 4200 Inverter 014-3330 Imbalance sensor W28 ×...

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