Evolve 200 Technical User Manual

Evolve 200 Technical User Manual

70mm, 100mm and 140mm chromatography columns
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70mm, 100mm and 140mm
Chromatography Columns

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Table of Contents
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Summarization of Contents

Column Hardware
Mechanical Description
Detailed explanation of the column's design and features.
Technical Data
Specifications including dimensions, volume, pressure ratings, and operating temperature.
Weights and Dimensions
Physical size and weight details for different column models.
Pressure Drop
Graphs illustrating pressure drop versus linear velocity for column models.
Material Conformity
Lists materials used for wetted and non-wetted parts, conforming to standards.
Chemical Compatibility
Tables showing resistance of column components to various chemicals.
Safety Guidelines
Pressure Equipment Directive (PED)
Information on compliance with European pressure equipment regulations.
Over-pressurization
Recommendations for preventing and managing over-pressurization.
Handling
Guidelines for safe manual and mechanical handling of columns.
Installation and Column Set-up
Installation
Initial steps for setting up the column after receipt.
Column Preparation
Procedures for preparing the column components before packing.
Installation of bed supports
Steps for correctly fitting the bed supports.
Installation of Fixed Bed Support
Detailed procedure for installing the fixed bed support.
Leveling the 70mm, 100mm & 140mm Columns
Instructions for ensuring the column is level before operation.
Installation of Adjuster Bed Support
Steps to install the adjuster bed support correctly.
Set Bed Height
Procedure for adjusting the bed height and energizing the seal.
Column Operation
Hydrostatic Leak Test
Procedure to check for leaks and ensure column readiness.
Media Preparation
General method for preparing chromatography media for packing.
Column Packing (Constant Flowrate Method)
Detailed instructions for packing the column using a constant flowrate.
Unpacking
Conventional method for unpacking the column.
Cleaning and Storage
Guidelines for cleaning and storing the column.
Maintenance
Routine Maintenance Procedures-General guidelines
General advice and precautions for column maintenance.
Disassembly of 70mm, 100mm and 140mm Columns
Step-by-step instructions for disassembling the column.
Reassembly of 70mm, 100mm and 140 column
Step-by-step instructions for reassembling the column.
Troubleshooting
Leakage from adjuster seal
Causes and solutions for leaks around the adjuster seal.
Leakage from fixed bed support seal
Causes and solutions for leaks from the fixed bed support seal.
Leakage from inlet or outlet
Troubleshooting steps for leaks at column connections.
Excessive column back pressure
Identifies causes and solutions for high back pressure.
Media Leakage
Addresses potential causes of media leakage from the column.
Kits, Spare parts and Accessories
Connection kit
Details of kits containing frequently used connectors.
Tool kit
Information on the included tool kit for column maintenance.
Spares Kit
Overview of the spares kit contents.
Accessories
Lists available accessory kits such as fluid connection and hose kits.
Appendix 1 Chromatography Performance
Performance Measurement and Column Efficiency
Explains methods for measuring chromatographic efficiency and calculations.
Performance Results From Evolve®Columns
Presents actual performance data for the columns with specific media.

Table of Contents