Table of Contents
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Table of Contents
Related Manuals for Evolve 140
Summarization of Contents
Column Hardware
Mechanical Description
Describes the design features and non-metallic flowpath of the Evolve Column for biopharmaceutical applications.
Column Sub-assemblies
Details the three main sub-assemblies of the Evolve column: adjuster, tube, and fixed assembly.
Technical Data
Technical Data Table
Presents detailed specifications like Cat No., dimensions, volume, pressure, and temperature for different column sizes.
Weights and Dimensions Table
Lists weights, maximum diameters, and minimum heights for various Evolve column models.
Pressure Drop
Pressure Drop Curves
Explains pressure drop and shows graphs for 70mm and 100mm columns at different flowrates.
Pressure Drop for 140mm Column
Displays the pressure drop graph for the Evolve 140mm chromatography column.
Material Conformity
Process-Wetted Parts Material
Lists materials in contact with the process stream, conforming to FDA and USP standards.
Non Process-Wetted Parts Material
Details materials used for external components of the column assembly.
Chemical Compatibility
Wetted Components Chemical Resistance
Provides resistance data for various chemicals with wetted column components.
Non-Wetted Components Chemical Resistance
Lists chemical resistance data for non-wetted stainless steel parts.
Safety Guidelines
Pressure Equipment Directive (PED) Compliance
Explains how the Evolve column complies with the European Pressure Equipment Directive 2014/68/EU.
Over-pressurization Prevention
Advises on preventing column over-pressurization and recommends protective devices.
Handling and Lifting Precautions
Provides guidance on safe handling of heavy column components, recommending mechanical aids.
Chemical Compatibility Considerations
Stresses checking compatibility for unlisted substances to maintain column integrity.
Installation and Column Set-up
Column Installation Overview
Outlines the steps for preparing and installing the column, including bed supports and leveling.
Installation of Bed Supports
Details how to install the polypropylene bed supports with Santoprene seals.
Leveling the Columns
Explains the process of leveling the 70mm, 100mm, and 140mm columns using a spirit level.
Installation of Adjuster Bed Support
Describes the steps for installing the adjuster bed support and seal.
Setting Bed Height
Details how to reinstate the adjuster unit and set the required bed height.
Column Operation
Hydrostatic Leak Test Procedure
Explains the two-step hydrostatic leak test to ensure column integrity and readiness for use.
Media Preparation Guidelines
Provides general guidance on preparing chromatography media, advising media manufacturer's recommendations.
Column Packing Method
Outlines the step-by-step process for packing the column using the constant flowrate method.
Column Schematic and Part Identification
Illustrates the Evolve column with a detailed schematic and part identification.
Column Packing Process Details
Continues packing instructions, including adjusting seal compression and removing residual air.
Cleaning and Storage Procedures
Provides guidelines for cleaning the column after use and recommended storage solutions.
Storage Precautions for Unpacked Columns
Advises on proper storage conditions for unpacked columns, avoiding sunlight and UV sterilization.
Maintenance
Routine Maintenance Guidelines
Offers general guidelines for column disassembly, assembly, and tool usage for maintenance.
Adjuster Assembly Disassembly
Provides step-by-step instructions for disassembling the adjuster unit of the column.
Fixed Assembly Disassembly
Details the procedure for safely disassembling the fixed assembly of the column.
Fixed Assembly Reassembly
Explains how to reassemble the fixed assembly, including torque settings for bolts.
Fixed Assembly Reassembly Torque Settings
Specifies the correct torque settings for reassembling the fixed assembly bolts.
Adjuster Assembly Reassembly
Details the process of reassembling the adjuster assembly, including seal and flowtube fitting.
Adjuster Assembly Reassembly Final Steps
Covers the final steps of adjuster assembly reassembly, including alignment and tightening.
Troubleshooting
Leakage from Adjuster Seal
Addresses common causes and solutions for leaks originating from the adjuster seal.
Leakage from Fixed Bed Support Seal
Provides troubleshooting steps for leaks related to the fixed bed support seal.
Leakage from Inlet or Outlet
Guides on identifying and resolving leaks at the column inlet or outlet connections.
Excessive Column Back Pressure
Discusses potential causes and checks for excessive back pressure in the column.
Media Leakage Causes and Solutions
Lists causes and checks for media leakage from the column.
Kits, Spare Parts, and Accessories
Connection Kit Contents
Lists the components included in the Connection Kit for the Evolve columns.
Tool Kit Contents
Details the items included in the Tool Kit, such as wrenches and spirit levels.
Spares Kit Contents
Lists the spare parts available for the Evolve columns, categorized by type.
Bed Support Kits
Details the specific bed support assemblies available as kits for different column sizes.
Elastomer Seal Kits
Lists the available kits containing adjuster and flow tube seals for various columns.
Wetted Flowpath Kits
Lists components for wetted flowpath kits, including adaptors, seals, and elbows.
Tube Kits (Replacement)
Details replacement tube kits, buffer rings, and scale labels for the columns.
Column Refresh Kits
Lists components for column refresh kits, including seals, cells, tubes, and labels.
Accessories
Fluid Connection Kits
Describes the contents of fluid connection kits for 70/100mm and 140/200/250mm columns.
Flexible Hose Kits
Lists flexible hose kits for different column sizes, including hoses, seals, and clamps.
Torque Wrench Kit
Details the torque wrench and fixed end tool included in the kit.
Contact Us
Contact Information and Support
Provides website, email, and physical address for further inquiries and support.
Appendix 1 Chromatography Performance
Performance Measurement and Column Efficiency
Explains how to measure chromatographic efficiency using HETP and theoretical plates (N).
Column Efficiency Calculation Methods
Details calculations for Asymmetry, Reduced Plate Height (RPH), and Dilution Factor.
Performance Results for Toyapearl Butyl 650M
Presents performance test results (N, Asymmetry) for Toyapearl Butyl 650M media on Evolve columns.
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