Related Manuals for DNA 11
Summarization of Contents
PRINCIPLES OF TEST
RNA preparation
Process of isolating total RNA from blood or bone marrow cells.
cDNA Synthesis
Process of synthesizing cDNA from isolated RNA using primers.
PCR
Amplification of cDNA using specific primers and DNA polymerase.
EQUIPMENT AND MATERIALS REQUIRED BUT NOT PROVIDED
RNA extraction
Required kit for RNA extraction from biological samples.
Reagent Module
Necessary reagent modules for the test procedure.
Master Mix room
Equipment and materials for the master mix preparation.
cDNA room
Equipment and materials for cDNA synthesis.
PCR room
Equipment and materials for PCR amplification.
Gel electrophoresis room
Equipment for visualizing PCR products.
PRECAUTIONS
General precautions
Important considerations for sample quality and handling to ensure accurate results.
Safety
Essential safety measures to follow during the assay.
PROCEDURE
Procedural notes
General guidelines and important considerations for performing the procedure.
RNA preparation
Detailed steps for preparing RNA samples before synthesis.
cDNA Synthesis and PCR
Steps for cDNA synthesis and subsequent PCR amplification.
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