1. Manuals
  2. Brands
  3. nanalysis Manuals
  4. Laboratory Equipment
  5. NMReady-60
  6. User manual

nanalysis NMReady-60 User Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the NMReady-60 and is the answer not in the manual?

Questions and answers

Related Manuals for nanalysis NMReady-60

Summary of Contents for nanalysis NMReady-60

  • Page 2 Nanalysis Corp. This book was carefully produced to describe the setup and operation of the NMReady-60 spectrometers. However, the authors do not claim that the information herein is completely free of errors. Readers are...

  • Page 3: Table Of Contents

    Laboratory temperature specification .................... 4 2.3.3 Supplying power to the Spectrometer .................... 4 2.3.4 Networking the Spectrometer ........................ 4 Powering up for the first time ...................... 6 Temperature Stabilization ......................6 Initializing the system ........................6 Regulatory Specifications ......................8 3.0 Acquiring NMR Data on the NMReady-60 ................9 Sample Preparation ......................... 9 NMReady Operation ......................... 9 3.2.1 Quick Select Buttons ........................... 10 3.2.2 Experiment Settings ............................ 12 4.0 Data Processing ........................ 3 7 1D Data Processing ........................ 3 7 4.1.1...
  • Page 4 10.7 Using the AUTOsample-60 ...................... 9 5 10.8 AUTOsample-60 workflow ...................... 9 6 10.9 Standby Shimming ......................... 9 8 10.10 Cancelling Experiments ...................... 9 8 10.11 Emergency Stop .......................... 9 8 10.12 Operation Guidelines ........................ 9 8 10.13 Hardware ............................ 9 9 10.13.1 Hardware Description .......................... 99 © 2019 Nanalysis Corp.
  • Page 5 10.13.2 Specifications ............................99 11.0 Frequently Asked Questions ..................... 100 11.1 General Queries ..........................100 11.1.1 Haven’t benchtop NMR spectrometers been around for years? ........ 1 00 11.1.2 Is the NMReady-60 spectrometer really a true benchtop NMR spectrometer? .. 1 00 11.1.3 How does it work? .......................... 1 00 11.1.4 How stable is the NMReady-60? Can I use signal averaging to improve the signal-to- noise ratio? .................................. 1 00 11.1.5 Can I look at flowing Samples? ...................... 1 01 11.1.6 Should I power down the spectrometer when I go home at night? ........ 1 01...

  • Page 6: Introduction

    Frequency. w = g The principal difference between the NMReady-60 and higher field superconducting magnets is the method used to generate the static magnetic field. Instead of generating a magnetic field using a cryogenically cooled superconducting solenoid, the NMReady uses carefully engineered permanent rare-earth magnets.

  • Page 7: Operation

    1.3 Using this Manual This document is a resource to facilitate the incorporation of the NMReady-60 into routine laboratory use. This manual contains: (1) instructions for installing the spectrometer (section 2) (2) specific details of the operational features of the NMReady interface (section 3 –...

  • Page 8: Getting Started

    NMR applications, it requires the use of very precise mechanical and electrical means. To ensure that these systems are not damaged upon shipment, the NMReady-60 is transported in a wooden crate and labeled as fragile. This helps to ensure that the unit is handled with care and that the instrument remains at the correct orientation.

  • Page 9: Laboratory Temperature Specification

    1) print directly to a network printer (section 6.4.2.2) 2) export data to a shared network folder (section 6.4.2.2) 3) remotely access the instrument (e.g., from a laptop) (section 6.4.2.2) 4) access advanced customer service over the Internet (section 6.5.1) © 2019 Nanalysis Corp.
  • Page 10 More information can be found regarding networking in section 6.4.2.2. Please note that Nanalysis Corp. will not be able to provide remote updates/customer support if you are unable to connect to the network. However, we can still provide assistance. Please submit offline inquires for customer support via email at service@nanaylsis.com.

  • Page 11: Powering Up For The First Time

    2.4 Powering up for the first time Once the location of the NMReady-60 has been designated, the first step is to plug the system either directly into the main power or into an uninterruptable power supply (UPS) that is plugged into the main power line.
  • Page 12 There is no need to remain in front of the spectrometer for the full duration of the installation process. Intermittent supervision, however, is recommended. While there is no need to contact Nanalysis’ customer service about this process, we do recommend contact us once it’s complete so it’s performance can be assessed.

  • Page 13: Regulatory Specifications

    Environmental rating: Standard Installation category: II Altitude: 2000 m Humidity: 0-90% (non-condensing) Electrical supply: 13.5 V DC Indoor use only Operating Temperature range: 18-26 °C Follows the: CE mark, the CSA mark and FCC Declaration of Conformity © 2019 Nanalysis Corp.

  • Page 14: Acquiring Nmr Data On The Nmready-60

    6. The present chapter describes basic operation of the NMReady, including selection of acquisition parameters and initiation of an acquisition. Depending on the model of NMReady-60 that you have purchased, the nuclei available under the Observe Nucleus button will differ. The NMReady-60e will provide the option of either H or ©...

  • Page 15: Quick Select Buttons

    H and either F or P as was specified by the user at the time of purchase. Unless requested the NMReady-60 uses a standard deuterium ( H) Lock Nucleus, but there is also a proton lock ( H) feature.
  • Page 16 For carbon detected experiments, for example basic experiment lists could include basic 1D, spin- lattice relaxation (T ), spin-spin relaxation (T ), Nutation (used to calculate the precise 90° pulse angle), Distortionless Enhancement by Polarization Transfer (DEPT), Attached Proton Test (APT), © 2019 Nanalysis Corp.

  • Page 17: Experiment Settings

    Custom and using the pop-up keyboard to input the exact number of scans you desire. The scans in the list are multiples of four as the NMReady-60 uses a phase alternating pulse sequence (PAPS), however it is not necessary to customize to a multiple of 4.
  • Page 18 (Note that some authors discussing this relation define "number of points" to be the number of real values obtained, which is twice the number of complex valued data points). For most applications, 0.05– 0.10 Hz resolution is more than sufficient unless you require very well-defined peak shapes, such as in a © 2019 Nanalysis Corp.
  • Page 19 Dummy scans permit the spin system to reach a steady state prior to acquisition of data. This parameter can be set to 0, 1, or 2 to allow for attainment of steady state, and this may be useful, particularly in 2D experiments. © 2019 Nanalysis Corp.
  • Page 20 90° pulse and detecting the FID (figure 2). The time interval between the pulses is incremented on successive iterations of the pulse sequence, and the data are collected into a matrix. Figure 2 : Pictorial depiction of COSY pulse sequence. © 2019 Nanalysis Corp.
  • Page 21 The appearance of cross peaks is evidence of proximity of the spins within the molecule. There are many COSY variants described in the NMR literature. The NMReady-60 runs a standard COSY-90 (i.e., X=90 in figure 2) and displays and stores its data in magnitude mode.
  • Page 22 0.1 ms is suitable for the majority of situations. The f Spectral Width is the width of J-couplings that will be displayed and, for most proton spectrum this should be between 20 and 60 Hz. Septets may require larger windows. © 2019 Nanalysis Corp.
  • Page 23 Trim-Pulse Time is the duration of the pulse applied at the beginning and end of the spin lock pulsing sequence. It is included in the t time. This pulse is used to remove magnetization that is transverse to the spin lock axis prior to the spin lock sequence. © 2019 Nanalysis Corp.
  • Page 24 200 ppm, centered at about 100 ppm, unless it is expected from chemical considerations to be narrower in the C domain. Figure 5 : Pictorial depiction of HSQC pulse sequence. © 2019 Nanalysis Corp.
  • Page 25 8. It can also be helpful to select 6 or 8 dummy scans to help reach a steady state magnetization for the sample. The NMReady-60 HSQC program includes an optional “BIRD” (BIlinear Rotation Decoupling) filter as part of the further suppression of the spurious...
  • Page 26 J-couplings in the proton spectrum is not as important as the location of the correlation peaks. The C spectral width and center is addressed by using the automatic 240 ppm specified in C Spectral Width and 100 ppm in © 2019 Nanalysis Corp.
  • Page 27 ~ 150 Hz coupling. For the NMReady-60 HMBC pulse program, the purge pulse is used to suppress the resonances from protons bonded to C. The length of that Purge Pulse , as well as the Purge Amplitude can be optimized as desired.
  • Page 28 It can also be helpful in studies of larger molecules, such as polymers, where it can correlate with timescales of molecular motion. Figure 7: Pictorial depiction of inversion recovery pulse. © 2019 Nanalysis Corp.
  • Page 29 The spin-spin, or transverse, relaxation time, (T ) quantifies the time required for the bulk magnetization to dephase after an RF pulse due to interactions with other spins. NMReady-60 spectrometers provide three types of experiments to measure T relaxation in a sample. The T...
  • Page 30 Once these parameters have been added, select Done to exit Experiment Settings and return to the main screen. From the main screen, select Go to initiate the experiment. © 2019 Nanalysis Corp.
  • Page 31 100 echoes, the second after 200 echoes, … and the tenth after 1.000/0.001 = 1000 echoes. (But note that each spectrum is an average of scans, the number of which is determined by the Number of Scans parameter specified in the top menu.) © 2019 Nanalysis Corp.
  • Page 32 For CPMG-FSE, after setting the Scan Delay , Spectral Center , Number of Scans , and Dummy Scans , one need only set the Total Time and Number of Echoes in the T2 - CPMG - FSE tab. © 2019 Nanalysis Corp.
  • Page 33 Start Width to the End Width in the defined by the Number of Steps . The NMReady-60 uses the 180° null as the point of reference so End Width should be greater than twice the expected 90°...
  • Page 34 The decoupling mode for a DEPT experiment should be Inverse Gated and the type of DEPT experiment can be setup by selecting the desired q Pulse Angle (45, 90 or 135). © 2019 Nanalysis Corp.
  • Page 35 Number of Points , Scan Delay , Number of Scans , Spectral Center , Dummy Scans ). The APT specific parameters are located on the bottom half of the page, where the Estimated J-coupling can be optimized for the molecule, depending on the nature of the structure. © 2019 Nanalysis Corp.
  • Page 36 As the HETCOR is carbon-detected, the top half of the experiment tab allows the user to modify the spectral parameters of the C NMR experiment ( Spectral Width , Number of Points , Scan Delay , © 2019 Nanalysis Corp.
  • Page 37 1D experiments that will be run and the frequency with which the will be acquired. The user can select: (1) Wait Type ; (2) Tau ; (3) Number of Clusters ; and (4) Geometric Factor . © 2019 Nanalysis Corp.
  • Page 38 1 minute, run second, wait 2 minutes, run third, wait 3 minutes, etc.). The Number of Clusters refers to how many times the specified 1D experiment is run; by selecting ’6’ there will be 6 1D experiments are run in succession with the defined time interval. © 2019 Nanalysis Corp.
  • Page 39 Pre-Sat suppression method will bring up two parameters that can be adjusted ( Suppression Amplitude and Suppression Length ). The suppression length is roughly inversely proportional the width of the peak being suppressed, and the amplitude controls the power of the pre-saturation pulse. © 2019 Nanalysis Corp.
  • Page 40 Amplitude Factor controls the amplitude of the pulses (as a percentage of the full-power 90 pulse). The Interpulse Delay is the time between pulses in the DANTE train, and the Final Delay is the length of the last delay that precedes the regular 90 pulse. © 2019 Nanalysis Corp.
  • Page 41 For an example of suppression please see the illustrative stacked plot below for a 60% vodka sample in 40% D 3.2.2.21 Experiment Designer This allows users to design their own pulse programs, customizing the number of pulses, timing, phases etc. For more information please contact your Nanalysis customer service representative. © 2019 Nanalysis Corp.

  • Page 42: Data Processing

    4.1.1.1 FID/FT Toggle On the top right-hand side of the spectrum will be an FID button. If pressed, instead of viewing the Fourier Transformed spectrum, the user can see the time domain data. © 2019 Nanalysis Corp.

  • Page 43: View

    Remember, this touchscreen does not respond to gestures like a smart phone (e.g., there is no two-finger “spread” feature). After selecting H Zoom, you need to touch the screen twice to define the left and right boundaries. © 2019 Nanalysis Corp.
  • Page 44 Then touch the bottom of the screen to highlight what you wish to include within the window. The spectrum will be then scaled accordingly. 4.1.2.3 Show All The Show All button restores the original size of the spectrum. © 2019 Nanalysis Corp.
  • Page 45 To assist in determining coupling constants, the Cursor function has a dual cursor display. The dual cursor display is enabled with a delta button, and the X1 and X2 toggle buttons alternatively lock the position of one or the other of the dual cursors for fine adjustment. © 2019 Nanalysis Corp.
  • Page 46 If desired, the user can put on a grid to help visualize the spectrum by selecting Grid. The spacing of the grid lines will depend upon the zoom of the spectrum, the more zoomed in the spectrum is the closer the lines will appear. © 2019 Nanalysis Corp.
  • Page 47 Simply select the Add Scans, input the number of scans you wish to add and hit Enter. This will continue the data acquisition. This button can be used after phasing, etc., although it is not required that the data be analyzed prior to continuing. © 2019 Nanalysis Corp.

  • Page 48: Process

    4.1.3.1 Phase The NMReady-60 has onboard tools to adjust the spectrum. The Phase can let you modify the spectrum either automatically or manually using the onscreen controls. To phase automatically, simply press Auto. The manual phase adjustment is performed using on-screen sliders by either dragging the cursor or tapping on the lighter green side rail.
  • Page 49 Baseline button and then by selecting Auto or manually by using the up and down arrows. If the autocorrect or manual correction does not improve the baseline, the Reset function restores the original baseline settings. © 2019 Nanalysis Corp.
  • Page 50 (e.g., if number of points is 1024 then setting zero filing to 1 will process 2048 points, setting it to 3 will process 4096 points. The instrument defaults to 7x zero fill, but by using the arrows, this can be changed to 0, 1, 3, 7 and 15, as is desired. © 2019 Nanalysis Corp.
  • Page 51 The Apply To… button becomes active when using the AUTOsample-60 (section 10.0) or queueing (section 7.1). This allows the user to apply the same Phase, Baseline, Apodize L/B and Zero Fill parameters to a series of spectra. © 2019 Nanalysis Corp.

  • Page 52: Analyze

    Show the threshold. When selected a green line will appear on the screen showing the lower threshold of the integration. Using the Up and Down arrows, you can include more or less of the peak in an integration region. This is the threshold Auto integration. © 2019 Nanalysis Corp.
  • Page 53 Set Reference will pop-up a keyboard. This will allow you to input the relative integral of that region. For example, the integral of a –CH – resonance can be set to 2, and the other integrals will adjust relatively. © 2019 Nanalysis Corp.
  • Page 54 4.1.4.3 Chemical Shift Reference The NMReady-60 monitors the deuterium channel to lock at the beginning of each scan to ensure that there is a minimal drift. This process affords reasonably accurate chemical shifts. However, to ensure that the observed chemical shifts are precise, they can be further referenced upon workup if desired.

  • Page 55: Data Processing

    This is shown for an HSQC, but the principle holds for all 2D data processing. The user should optimize the threshold to see the maximum number of correlations without making either the cross peaks or noise artifacts (e.g., T noise) too dominant. © 2019 Nanalysis Corp.

  • Page 56: Process

    100 Hz in both dimensions. Like in the 1D spectra, increasing the apodization reduces noise artifacts, but it does so at the expense of fine resolution of points. Applying a negative apodization or increasing the zero fill will help the user see observe better resolution in both dimensions. © 2019 Nanalysis Corp.

  • Page 57: Saving And Exporting Data

    5.0 Saving and Exporting Data After data processing is complete, the data can be saved directly to the NMReady-60 (as either a dx, pdf or a png file, see section 6.3.2 and 5.1), exported to a network folder or sent to a network printer once those features have been enabled.

  • Page 58: Print

    When the printer icon is selected, a Print Details window will appear allowing the user to customize fields including Title , Sub-title , Author(s) and Description and selected the desire Printer , Paper Size and Orientation . © 2019 Nanalysis Corp.
  • Page 59 The user can check the PDF box if they’d like to save as a pdf. The image will print in a frame and will be accompanied by experimental parameters. In order to ensure that your peak picks and integrations are accurate, ensure that the threshold is positioned correctly for each spectrum. © 2019 Nanalysis Corp.

  • Page 60: Nanalysis User Interface: The Task Bar

    User Manual 6.0 Nanalysis User Interface: The Task Bar The main screen of the NMReady-60 user interface is divided into two sections from which the spectrometer can be operated. 1) The task bar is located at the top of the main screen and can be accessed from most windows in the user interface.

  • Page 61: Temperatures

    6.2.1 Temperatures The NMReady-60 tracks the temperature of the: (1) electronics board; (2) magnet; and (3) enclosure. Fluctuations are expected within the boards and enclosure, but the magnet temperature should remain constant.

  • Page 62: Versions

    6.2.7 Messages When a warning appears on the Status button, the NMReady-60 will provide a recommendation to improve the overall performance of the instrument. These are based on numerous measurements of system parameters and performance characteristics.

  • Page 63: Results

    6.2.8.3 Full Finally, a full shim performs a full electronic tune-up. For the NMReady-60 this shims down to fourth order gradients. It takes approximately 20 minutes to complete and will be recommended by the system status function every 24 hours.
  • Page 64 Please note that based on the file type, only the relevant buttons will be enabled (e.g., .png files cannot be loaded back into the NMReady-60 processing window for further processing and therefore Load will remain greyed out).

  • Page 65: Load

    The set-up of this folder will be further discussed in section 6.4.2.2. To export a file to a USB drive, the USB key must be inserted into the NMReady-60. This will render the Export to USB and Eject USB keys active. Next, select the desired file and touch Export to USB button.

  • Page 66: Queuing

    On or Off by toggling the button, similarly one can choose to share generic usage statistics with Nanalysis with an On or Off toggle. There is also an option to skip the pre scan delay. It is recommended that the user does not use this.
  • Page 67 Any time the screen has gone to sleep, touching it will turn it back on. 6.4.2.2 Network Settings As discussed in section 2.3.4 connecting the NMReady-60 to a network enables: (1) access to a network folder for exporting data; (2) using a network printer; (3) software updates; and (4) advanced customer service.
  • Page 68 If Ethernet is not available, but Wi-Fi is available, a Wireless configuration tab is also available. To find a wireless network, select Scan, to select the Wi-Fi network that you would like to connect to. Then enter the password and select Connect. © 2019 Nanalysis Corp.
  • Page 69 2. Go to the screen Setup è System è Network è Configuration 3. Enter in the IP address supplied by the network administrator along with the netmask, gateway and nameserver. 4. Double check that addresses have been entered correctly 5. Press the Apply button © 2019 Nanalysis Corp.
  • Page 70 Test the path to the shared folder to make sure it is working (press Test button) Once this is done, you can press the Export to Network button on the results screen and quickly upload files to the shared folder. © 2019 Nanalysis Corp.
  • Page 71 This functionality is not included in the basic instrument package, but if enabled can be easily accessed from Setup è System è Network è Remote. If you are interested in more information about this functionality, please visit the product page (Nanalysis.com/nmready-connect), login to the developer website (Nanalysis.com/developer-zone) or notify your Nanalysis representative.
  • Page 72 The desired printer can be selected by highlighting it and then hitting Add. Again, to ensure the printer is properly configured, the NMReady-60 must be restarted after the printer installation. To restart, click the power button on the right-hand side of the NMReady-60, chose Restart and wait for the instrument to reboot itself.
  • Page 73 Date/Time This tab enables the date and time of the NMReady-60 to be changed depending on the time zone you are in. If you are without a network and find that the time is not correct, it can be modified by hour, minute and second as is necessary.
  • Page 74 To restart, click on the power button on the right-hand side of the NMReady, choose Restart and wait for the instrument to reboot itself. The NMReady-60 is defaulted to lock on deuterated solvents, although non-deutero options are also available.

  • Page 75: Shim History Tab

    The Shim History Tab displays a chronological list of the most recent shims that were applied to the NMReady-60. Both auto and manual shims are included in this list. To re-apply an old shim set, select the desired entry in the list and touch the Load Shims button. It is rare that recalling a shim set from the shim history is required.

  • Page 76: Autoshim Tab

    (1) choosing the gradient to be tuned (e.g., B ), these will appear in the ‘Shims currently selected’ box; and (2) pressing Start Autoshim. The estimated time it will take to autoshim will appear next to this. © 2019 Nanalysis Corp.

  • Page 77: Manual Shim Tab

    Consequently, the window loads with a warning to inform users that any changes made in this screen can be detrimental to the performance of the NMReady-60 and should be used with caution. © 2019 Nanalysis Corp.

  • Page 78: Service Tab

    The Service tab contains very powerful operations that govern the configuration of the NMReady-60 and is primarily intended for use by Nanalysis service personnel. It can be restricted by a separate PIN access than the Setup panel to prevent unauthorized users from accessing this screen. There are four subtabs (1) Maintenance;...
  • Page 79 6.4.6.2 Tests The Tests tab is meant primarily as a diagnostic tool for Nanalysis Customer Service representatives to assess the radiofrequency system, the instrument, and temperature performance over time. This allows them to perform diagnostic testing using the Shim Test, Stability Test, RF Self Test 1, RF Self Test 2, System Test and OS Test.
  • Page 80 6.4.6.3 Updates The Updates tab is meant to simplify software and firmware updates. Nanalysis will notify you when it is necessary to update the software. It can be done either through the Ethernet network connection, or by putting the updated files on a USB key. When a software update is selected, the software will automatically detect any versions that are higher than that being run on the spectrometer.
  • Page 81 Additionally, one can access the Service Screen or alter Digital Filters but these are not recommended without guidance from a Customer Service Representative. Of note is the Diagnostic Utility. This utility is used for troubleshooting, backing up files, updating the instrument software and uploading © 2019 Nanalysis Corp.
  • Page 82 NMReady-60 User Manual logs to Nanalysis. If selected, or if the instrument detects an error, the utility will be launched automatically and the glow ring will turn red and enter the diagnostic screen. © 2019 Nanalysis Corp.

  • Page 83: Help

    OS. 6.5 Help The Help Panel is present in virtually every window of the NMReady-60 interface. It can be used to offer assistance for proper operation of your spectrometer, as well as for diagnosis and fixing issues that may arise during routine use.

  • Page 84: Customer Service Agent

    Share my screen with customer service is activated. The Stop sharing option will remain in the top right-hand corner of the screen for the entire duration in the spectrometer is connected to the Nanalysis service network. To terminate the connection at any time, simply press Stop sharing.
  • Page 85 NMReady-60 User Manual © 2019 Nanalysis Corp.

  • Page 86: Optional Software Packages

    If Queuing is enabled on a spectrometer an orange ‘+’ will appear in the right-hand corner of the Go button. To set-up a queue: 1. Select the desired Observe Nucleus (choices depend on which NMReady-60 model you have) Lock Nucleus ( H or H lock), Solvent, and Experiment on the front screen.

  • Page 87: Installation Qualification And Operational Qualification (Iq

    7.2 Installation Qualification and Operational Qualification (IQ/OQ) Adherence to regulatory compliance is critical in many work places, especially with contract research and manufacturing organizations (CRO and CMO). Nanalysis offers an optional software package for Installation Qualification (IQ) and Operational Qualification (OQ). These packages test the instrument’s performance on initial siting, as well as in routine workflow to help validate result integrity over time.
  • Page 88 Once this is manually completed by the user, and OK is selected and the procedure will begin to immediately perform the series of tests. © 2019 Nanalysis Corp.
  • Page 89 IP address as well as ensures that the necessary ports are open. As each test is completed, it will receive a pass or fail based upon the specified parameters. Example progress parameters are shown below. © 2019 Nanalysis Corp.
  • Page 90 5 minutes to complete. When completed the Report button becomes active and a report can be generated. © 2019 Nanalysis Corp.

  • Page 91: Experiment Designer

    NMReady-60 User Manual 7.3 Experiment Designer For more information on the experiment designer and customizing your own pulse programs, please contact your Nanalysis customer service representative. © 2019 Nanalysis Corp.

  • Page 92: Shimming On The Nmready

    It is used to lock and provide evidence of B homogeneity and instrument performance. The NMReady-60 is preset with basic shimming protocols (e.g., quick, medium and full, see section 6.2.8 and more advanced auto-shim (section 6.4.4) and manual shim (section 6.4.5) options.

  • Page 93: Hardware Description

    8.4.5 Progress Ring This has been introduced to the NMReady-60 so the user has an easy visual identification of the progress of an experiment and the status of the instrument. The process ring can be disabled in the Setup è...

  • Page 94: Specifications, Recommendations And Guidelines

    The colour blue indicates that the NMReady-60 is still in use, whether that means it is shimming or collecting data. A green light ring means that the NMReady-60 is ready for use. A red light ring means there was an error and the spectrometer requires some diagnostic maintenance.

  • Page 95: Maintenance And Troubleshooting

    Please note that the broken NMR tube can be sharp and could hold remnants of dangerous materials. Please exercise caution when removing broken NMR tubes by using appropriate tools and/or personal protection equipment. © 2019 Nanalysis Corp.

  • Page 96: Changing The Air Filter

    4) Reset the air filter reminder by pushing the Reset Air Filter Reminder button, which is found on the Maintenance tab under Setup è Service. © 2019 Nanalysis Corp.

  • Page 97: Autosample-60

    10.0 AUTOsample-60 10.1 Introduction The AUTOsample-60 is designed as an accessory to the NMReady-60 family of benchtop spectrometers, to improve workflow and allow for automated sample tube changing. The AUTOsample-60 can help automate NMR spectrometer experiment workflow when more than one sample is used.

  • Page 98: Using This Manual

    2 carousels 1 USB cable 1 24V external power supply Removing the middle foam insert will expose the AUTOsample-60, which can be removed by grasping the unit from its sides. power supply carousels power supply AUTOsample © 2019 Nanalysis Corp.

  • Page 99: Installing The Autosample-60

    To install the AUTOsample-60 onto your NMReady, first remove the sample tube from the NMReady, grasp the AUTOsample-60 by its sides, and place it onto the round top cover of the NMReady-60. The top cover of the NMReady-60 mates to the bottom of the AUTOsample-60 and locates the two instrument pieces together.

  • Page 100: Moving The Autosample-60

    AUTOsample-60 and use it to change to the desired tube. Note that the NMReady-60 must have the AUTOsample-60 feature enabled in its license. When the AUTOsample-60 feature is enabled on the NMReady, the front screen will have a button for “Tube Number”.

  • Page 101: Autosample-60 Workflow

    To queue experiments using different tubes, the procedure is similar to queuing (section 7.1), but the user must also specify the Tube Number: 1. Select the desired Observe Nucleus (choices depend on which NMReady-60 model you have) Lock Nucleus (...
  • Page 102 AUTOsample-60 tube warmer 4. The NMReady-60 will perform the current experiment(s) on the first NMR sample 5. After the current experiment is completed, the sample is moved back to its original location in the carousel 6.

  • Page 103: Standby Shimming

    User Manual 10.9 Standby Shimming The NMReady-60 has a standby shimming protocol that can be used to maintain optimal line shape through times when the instrument is not being used. The Standby Shimming button is in the upper left-hand corner of the main screen ( ).

  • Page 104: Hardware

    The motors have been factory calibrated and require no on-site calibration or maintenance. 10.13.2 Specifications Power 24 V DC via power adapter Weight 10 lbs / 4.5 kg Connectivity Height* 12.5 inches / 32 cm *Above the top of the NMReady-60 © 2019 Nanalysis Corp.

  • Page 105: Frequently Asked Questions

    All of the components are packaged in a single enclosure. 11.1.4 How stable is the NMReady-60? Can I use signal averaging to improve the signal-to- noise ratio? The NMReady uses a deuterium (or proton) internal lock to stabilize the transmitter position in the magnetic field.
  • Page 106 11.1.5 Can I look at flowing Samples? The basic design of the NMReady magnet is compatible with custom sample-flow systems! We offer a flow accessory, please inquire with your local Nanalysis representative about the NMReady-flow to use your NMReady as on online NMR detector.
  • Page 107 2 minutes in the magnet before acquiring NMR data on the sample. 11.2.4 What experiments can I do? All the NMReady-60 instruments are configured with a standard one-pulse 1D experiment, a nutation experiment for pulse calibrations, standard T...
  • Page 108 H NMR of small molecule work (< 400 Da or so) if a spectrum is required in a few scans (< 30 sec). If you are sample limited but not time limited, lower concentrations are fine, but they will require more signal averaging. © 2019 Nanalysis Corp.

Table of Contents