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Clarity ® , DataApex ® and ® are trademarks of DataApex Ltd. Microsoft ® and Windows TM are trademarks of Microsoft Corporation. DataApex reserves the right to make changes to manuals without prior notice. Updated manuals can be downloaded from www.dataapex.com. Author: DM...
Jasco FP-4020/4025 Table of Contents Contents 1 Jasco FP-4020/4025 Control Module 2 Requirements 3 Installation Procedure 3.1 Jasco FP-4020/4025 detector communication 3.2 Clarity Configuration 4 Using the control module 4.1 Jasco FP-4020/4025 Setup 4.2 Method Setup - Acquisition 4.3 Time Program: Functions 4.4 Device Monitor 5 Troubleshooting 5.1 Error Messages during Operation...
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Jasco FP-4020/4025 Table of Contents To facilitate the orientation in the Jasco FP- 4020/4025 manual and Clarity chromatography station, different fonts are used throughout the manual. Meanings of these fonts are: Instrument (blue text) marks the name of the window to which the text refers. Open File (italics) describes the commands and names of fields in Clarity, parameters that can be entered into them or a window or dialog name (when you already are in the topic describing the window).
Jasco FP-4020/4025 1 Jasco FP-4020/4025 Control Module 1 Jasco FP-4020/4025 Control Module This manual describes the setting of the Jasco FP- 4020/4025 Fluorescence detector. The control module enable direct control of the instrument over RS-232 connection. Fig 1: Jasco FP-4020/4025 detector Direct control means that the detector can be completely controlled from the Clarity environment, including the digital data acquisition.
Jasco FP-4020/4025 2 Requirements 2 Requirements Clarity Installation USB with appropriate control license allowed - LC Control (p/n A24). Optional RS- 232 interface (Jasco Product Item: 6890- H445A, RS232C 9P CABLE WITH ADAPTER) Free COM port in the PC. Serial DB9M-DB9M cross cable (p/n SK01) Minimal firmware version 1.02.04 Note: Modern computers usually have only one (if any) serial (COM) port...
Jasco FP-4020/4025 3 Installation Procedure 3 Installation Procedure 3.1 Jasco FP-4020/4025 detector communication The Jasco FP-4020/4025 is controlled by serial (RS-232) communication. It uses a standard serial DB9F-DB9F cross cable (p/n SK01) described in the picture below.. Caution: RS-232 communication interface for the Jasco FP-4020/4025is optional (Jasco Product Item: 6890- H445A, RS232C 9P CABLE WITH ADAPTER).
Jasco FP-4020/4025 3 Installation Procedure 3.2 Clarity Configuration Fig 3: How to Add Jasco FP-4020/4025 module Start the Clarity station by clicking on the icon on the desktop. Invoke the System Configuration dialog accessible from the Clarity window using the System - Configuration... command. Press the Add button (①...
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Jasco FP-4020/4025 3 Installation Procedure Select the correct item and press the Add (③ on Fig 3 on pg 4.) button. Jasco FP-4020/4025 Setup dialog will appear. Set the correct communication Port and click on the Autodetect button to establish communication with the device. ④...
Jasco FP-4020/4025 4 Using the control module 4 Using the control module After adding and setting up the detector a new Acquisition tab will appear in the Method Setup dialog. A new Jasco FP-4020/4025 detector section enabling the monitoring of the current detector state will be also created in Device Monitor window.
Jasco FP-4020/4025 4 Using the control module 4.1 Jasco FP-4020/4025 Setup Fig 4: Jasco FP-4020/4025 Setup Port Select the communication port the instrument is connected to. AutoDetect Test the connection to the detector using the set COM port. Upload the configuration from the instrument: serial number, model type, firmware version, A/Z and mark in input, wavelength range, and flow cell ID reading settings.
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Jasco FP-4020/4025 4 Using the control module for a long period (5 minutes or more) may damage the photomultiplier tube. Note: Refer to the FP- 4020/4025 Fluorescence Detector Operator's Manual for details. Flow Cell ID Reading Shows whether the flow cell information data is read or not. Note that the flow cell temperature is not controlled if Inactive is set (FP-4020 model).
Jasco FP-4020/4025 4 Using the control module 4.2 Method Setup - Acquisition Method Setup - Acquisition tab serves for setting the common parameters of the Jasco FP- 4020/4025 detector. If more than one detector is available, it is possible to select between them by using the Select Detector combobox on the top of the dialog.
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Jasco FP-4020/4025 4 Using the control module EX1, EM1 Sets the first pair of excitation and emission wavelengths (nm) in the dual detection mode or ratio detection mode. When the detection mode is Single, the excitation and emission wavelengths (nm) can also be set in these fields.
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Jasco FP-4020/4025 4 Using the control module Offset values of Signal 1 and Signal 2 can be set in advance to suppress generation of negative values due to baseline drift in the ratio calculation. Autozero Mode Sets the autozero execution conditions. You can choose one of the following mode: Auto Autozero is executed automatically when the wavelength or gain is...
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Jasco FP-4020/4025 4 Using the control module Note: There is no stabilization time setting, the instrument will be ready when the flow cell temperature setpoint +/- 1°C is reached. Key Lock When this setting is enabled, key operation is disabled on the instrument. Time Program The time table is used to perform changes on the detector during run.
Jasco FP-4020/4025 4 Using the control module 4.3 Time Program: Functions The following table summarizes the settings that can be changed during the run according to the detection mode. Select first the detection mode, then add some steps in the time program table: Click Add to add a line at the end of the time program table.
Jasco FP-4020/4025 4 Using the control module 4.4 Device Monitor The window with the detector status can be invoked by the Monitor - Device Monitor command from the Instrument window or using the Device Monitor icon. It displays the detector global status and some actual detector settings.
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Jasco FP-4020/4025 4 Using the control module Displays the second actual set excitation wavelength ( Dual or Ratio detection mode) in nm. Displays the second actual set emission wavelength ( Dual or Ratio detection mode) in nm. EM Slit Width Displays the actual set emission slit width (in nm) for the emission side of the monochromator.
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Jasco FP-4020/4025 4 Using the control module Click Shut down or select Control / Shut Down to put the detector in safe mode and switch the lamp off even if an analysis is running. - 16 -...
When the solution to a problem cannot be found easily, a recording of the communication between Clarity and the detector will significantly help DataApex support. The data recording can be enabled by adding or amending the COMMDRV.INI file in the Clarity installation directory (C:\CLARITY\CFG by default).
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Jasco FP-4020/4025 5 Troubleshooting Solution: The excitation grating drive function is not carried out properly. Turn off the power, wait at least 10 seconds then turn on the detector again. Contact your local instrument distributor if the same error is still reported. ▌...
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Jasco FP-4020/4025 5 Troubleshooting ▌ An error message “PMT temperature error”. Solution: This is displayed when an error is detected in the measured temperature of the photomultiplier tube. Turn off the power, wait at least 10 seconds then turn on the detector again.
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