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GRIDION: PROTOCOL GridION Q user manual Q_GRD_revC_06Jan2025 This document will help you to install, check and configure the GridION™ Q device. For Research Use Only FOR RESEARCH USE ONLY...
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Contents Overview 1. Device information Installation 2. What's in the box 3. Installing and powering on the device Shutting down 4. Shutting down...
1. Device information The GridION Q The GridION Q is a compact benchtop device designed to run up to five MinION flow cells. Each flow cell is independently addressable, meaning that experiments can be run concurrently or individually. Integrated compute...
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Designed to sequence at +18°C to +25°C conditions Functional range of electronics +5°C to +40°C GridION Q information Further information about the GridION Q can be found in the following documents in the Q-line channel (https://community.nanoporetech.com/qline/): GridION Q IT requirements GridION Q technical specification...
2. What's in the box GridION Q packaging What's in the box The shipment will contain: GridION Q device Five country-specific C13 cables Five configuration test cells (CTCs) DisplayPort to HDMI adapter Quick Start Guide...
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The components of the GridION Q are shown below.
The connections and ports available on the GridION Q are shown below. 3. Installing and powering on the device Preparing for installation Before you install the device, the following must be available: 1x Ethernet cable (1 Gbps) Ethernet port providing outbound-only access to the internet over port 443, and TCP access to AWS eu- west-1 IP ranges listed here for EPI2ME analysis Outbound-only access over HTTPS/port 443 to 52.17.110.146, 52.31.111.95, 79.125.100.3 or DNS rule for...
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Optional: optical barcode scanner Recommended laboratory location Place the device on a strong, clean laboratory bench Ensure Ethernet and power connectors are in close proximity Ensure power socket is easily accessible should a disconnection be required in case of emergency Sequencing should be carried out at a room temperature of +18°C to +25°C Allow 30 cm clearance to rear and sides of the device Do not cover any ventilation grilles...
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1x C13 cable *1x DisplayPort to HDMI adapter is provided Connect the power cable to your mains/UPS solution. Use the mains cord supplied, or a suitable approved and rated power cord. Ensure the device is connected to a protective earth mains supply. Turn the power source on. Press the power button.
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Log into the GridION. Click on GridION user.
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grid You will be prompted for the password. Enter the password: Double-click the sequencing software icon located on the desktop.
The GridION's computer requires a stepwise, processed shut down, otherwise there may be problems e.g. when recognising flow cells. Follow the instructions in this section to ensure you do not face errors with your GridION Q from incorrectly shutting down your device.
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If an assay is running, you will see "Run in progress" underneath individual positions.
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Close the sequencing software. In the top right corner of the screen, click the power button, then click "Power Off/Log Out" Click "Power Off". Then confirm by clicking Power Off in the dialog box. If not clicked, the device will automatically power off after 60...
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Turn off the device at the mains supply, if you are using mains power.
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