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DTI TCST-9622 Operation Manual

Dti fabspeed thermal cycler
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DTI FabSpeed Thermal Cycler
Model
TCST-9622
Operation Manual Ver 1.0

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Summary of Contents for DTI TCST-9622

  • Page 1 DTI FabSpeed Thermal Cycler Model TCST-9622 Operation Manual Ver 1.0...

  • Page 2: Table Of Contents

    TCST-9622 Operation Manual Table of Contents Contents 1. Safety Precautions ...................... 4 1.1. Intended Use ......................4 1.2. Safety Label ....................... 4 1.3. General Instrument Safety ..................4 1.4. Chemical Safety ......................5 1.5. Chemical Waste Safety ..................... 6 1.6. Electrical Safety ......................6 2.
  • Page 3 TCST-9622 Operation Manual 6.4. Terminating a Running Protocol ................25 7. Tools ........................... 26 7.1. Tm Calculator ....................26 7.2. Incubator ......................26 7.3. Copy Number Convertor ................. 26 7.4. Master mix Preparation Wizard ..............26 7.5. Help ........................26 8.

  • Page 4: Safety Precautions

    1. Safety Precautions 1. Safety Precautions Before using the DTI FabSpeed Thermal Cycler for the first time, please read this entire operation manual carefully. To guarantee problem free, safe operation of the DTI FabSpeed Thermal Cycler, it is essential to observe the following section.

  • Page 5: Chemical Safety

    TCST-9622 Operation Manual 1. Safety Precautions Make sure the power source conforms to the required power supply specifications. To avoid electric shock, make sure the device is plugged into a grounded electrical outlet. 10. Do not allow water or any foreign objects to enter the various openings of the device.

  • Page 6: Chemical Waste Safety

    1.6. Electrical Safety ELECTRICAL SHOCK HAZARD. Severe electrical shock can result from operating the DTI FabSpeed Thermal Cycler without its instrument panels in place. Do not remove instrument panels. High-voltage contacts are exposed when instrument panels are removed from the instrument.

  • Page 7: General Description

    2. General Description 2. General Description The DTI FabSpeed Thermal Cycler is a powerful, reliable and affordable thermal cycler. It is equipped with a gradient function that enables the user to set a maximum 30°C temperature gradient between the left and right rows in the sample block at each temperature.

  • Page 8: Product Overview

    TCST-9622 Operation Manual 2. General Description 2.2. Product Overview Lid Handle Front Panel USB Port Air Vents Figure 1. Top view Table 1. Detailed description for top view Name Function 7” high resolution color LCD display with capacitive touch panel. It displays Front Panel the status of the system and allows the user to operate the instrument.
  • Page 9 TCST-9622 Operation Manual 2. General Description High Temperature Warning Label Lid Heater Platen Sample Block Figure 2. Top view with opened lid Table 2. Detailed description for top view with opened lid Name Function During and after PCR operation, the lid heater platen and the sample block High Temperature may become very hot (up to 120°C).
  • Page 10 TCST-9622 Operation Manual 2. General Description Product Product Label webpage QR Code Air Vents Power Cable Socket Power Switch Figure 3. Rear view Table 3. Detailed description for rear view Name Function Power Cable Socket Power cable socket and fuse compartment.

  • Page 11: Getting Started

    Switch on the device using the power switch at the rear of the device. The LCD display will show the boot screen and then the “DTI FabSpeed Thermal Cycler” title will be displayed. Tap on the “DTI FabSpeed Thermal Cycler” title to log into the Main Screen and start your operation. Tap on the “DTI FabSpeed Thermal Cycler”...

  • Page 12: Lid Opening/Closing

    TCST-9622 Operation Manual 3. Getting Started 3.3. Lid Opening/Closing To open the lid, pull the handle upward and lift the lid backward as shown in Figure 4 to the upright position. Figure 4. Opening the lid To close the lid, pull the lid downward and close the handle to the lock position as shown in Figure 5 Figure 5.

  • Page 13: Heated Lid

    Note If DTI FabSpeed Thermal Cycler is started cold, approximately two to three minutes will be required for the heater platen to reach the setting temperature. If the low-profile reaction vessels and the 5.2mm compression mat are used, an extra three minutes will be required to allow the compression mat to be heated to the required 120°C temperature.

  • Page 14: Loading The Reaction Vessel

    3. Getting Started 3.5. Loading the Reaction Vessel For the optimal performance of the DTI FabSpeed Thermal Cycler, the recommended sample volume for 0.2 ml tubes or 96-well plates is 5-100μl. For low profile vessels, it is required to use a 5.2 mm Compression Mat which will help placing even and sufficient pressure on the caps or sealing...

  • Page 15: Main Screen

    3. Getting Started 3.6. Main Screen On the Main Screen there are some information items indicate the status of the DTI FabSpeed Thermal Cycler, it also contains 8 main function icons. Please refer to the following Figure 8 and Table 5 for the detailed description.

  • Page 16: User Folder Management

    TCST-9622 Operation Manual 4. User Folder Management 4. User Folder Management Tap on the User icon on the main screen to enter the User folder management screen. A sample screen is shown in Figure 9 below. Lid:25 C Block:25 Lock symbol Figure 9.

  • Page 17: Editing A User Folder

    It is recommended to format your flash drive in FAT or FAT32 file system prior to using it on DTI FabSpeed Thermal Cycler) for the DTI FabSpeed Thermal Cycler to recognize it and create the required folder in it. Tap on the USB icon to open the specific folder (\\TPCR_Files) in the flash drive and use it as a User Folder.

  • Page 18: Protocol Management

    TCST-9622 Operation Manual 5. Protocol Management 5. Protocol Management After entering the selected User Folder, the protocol list will be displayed. A sample screen is shown as Figure 10 below. Users can utilize the function icons on the lower part of the screen to edit, create, delete, duplicate (save as new protocol) and open the protocols.
  • Page 19 TCST-9622 Operation Manual 5. Protocol Management 5.1.1. Changing Temperature/Time/Ramp Rate Setting 1. To select the Step, tap on the step segment. The selected Step will be highlighted and the Step Temperature, Time and Ramp Rate will be displayed. Tap on...
  • Page 20 TCST-9622 Operation Manual 5. Protocol Management 5.1.3. Changing Stage Cycle Number Stage 2 If a Stage contains more than one Step segment, the Stage Cycle Number will appear when 20 cycles you tap on the Stage header to highlight it. The Stage Cycle Number indicates the total number of Stage 2 times the Stage will repeat itself during the protocol running.
  • Page 21 TCST-9622 Operation Manual 5. Protocol Management Gradient Temperature Setting 5.1.4.1. Tap on the Temperature Gradient setting function icon, spin the wheel to the desired value for the gradient temperature span from the original temperature of the Step. The value 00.0 turns off the Gradient.

  • Page 22: Saving A Protocol

    TCST-9622 Operation Manual 5. Protocol Management 5.2. Saving a Protocol After all settings are entered, tap on the Save icon to store the protocol. Input the protocol name in the pop-up screen and tap on “OK”. If you create your new protocol from the Main Screen, you’ll then be brought to the User folder selection screen.

  • Page 23: Protocol Running Management

    TCST-9622 Operation Manual 6. Protocol Running Management 6. Protocol Running Management Caution: During the protocol running, the lid heater and the sample block may become very hot. Please be aware of the high temperature. 6.1. Running a Protocol Please refer to Section 5.3 Editing (Viewing) a Protocol to open and check the setting of a Protocol.

  • Page 24: Monitoring The Protocol Running Status

    TCST-9622 Operation Manual 6. Protocol Running Management Lid:25 C Block:25 Figure 16. Sample Block control mode Tap on Run icon to start the run. 6.2. Monitoring the Protocol Running Status During the protocol running, the running status screen will be displayed as shown in Figure 17 below.

  • Page 25: Pausing A Running Protocol

    TCST-9622 Operation Manual 6. Protocol Running Management 6.3. Pausing a Running Protocol To pause a running Protocol, tap on the Pause icon , a dialog box will pop up, requesting you to confirm the operation. The protocol running will not be interrupted before your confirmation. Tap to confirm and start the pause.

  • Page 26: Tools

    7.1. Calculate the Tm, length, GC% and the molecular weight for the input primer. Incubator 7.2. Allows you to use DTI FabSpeed Thermal Cycler as an incubator. Copy Number Convertor 7.3. Convert the DNA concentration into copy number. Master mix Preparation Wizard 7.4.

  • Page 27: System Setup

    8. System Setup Tap on the System icon on the Main Screen to enter the System setup section as shown in Figure 20 below. Here you can adjust several parameters for the DTI FabSpeed Thermal Cycler. Figure 20. System overview Brightness 8.1.

  • Page 28: Maintenance

    Air vents should be vacuumed to remove dust. 9.2. Cleaning the Heated Lid Make sure the DTI FabSpeed Thermal Cycler is turned off, unplugged and cooled down. Use mild detergents to clean material from the lid. A Kimwipe™ dipped in 70% ethanol will help remove residue from the sealing tape.

  • Page 29: Troubleshooting

    TCST-9622 Operation Manual 10. Troubleshooting 10. Troubleshooting Problem Cause Action During the protocol Operating temperature environment Operate the unit in a temperature running, the running may be unsuitable. environment between 15 to 30°C. status screen show warning icon. Power is not reaching the system.

  • Page 30: Error Messages

    TCST-9622 Operation Manual 10. Troubleshooting 10.1. Error Messages Message Cause Action Error 1: Ambient The room temperature exceeds Cool down the room temperature. temperature too high! 30°C. Reboot the unit There are no enough space in the Keep the front and rear of the...
  • Page 31 TCST-9622 Operation Manual 10. Troubleshooting N= 1 (UL) : upper left 2 (UC) : upper center 3 (UR) : upper right 4 (LL) : lower left 5 (LC) : lower center 6 (LR) : lower right Error 20~25: Block N (XX)

  • Page 32: Appendix A: Technical Specifications

    Horizontal across the block Gradient Temperature Range 30 ~ 100°C Gradient Temperature Difference Max. span 30°C Software Save and transfer to another DTI FabSpeed Thermal Portability of protocols Cycler via USB flash drive Stored program no. > 4000 sets Registered user folder no.

  • Page 33: Appendix B: Template Protocols

    Appendix B: Template Protocols 2-Step Stage 1 94°C, 5 min Stage 2 94°C, 30 sec 30 cycles 67°C, 30 sec Stage 3 72°C, 7 min Stage 4 4°C, Hold 3-Step Stage 1 94°C, 5 min 94°C, 30 sec Stage 2 55°C, 30 sec 30 cycles 72°C, 30 sec...
  • Page 34 TCST-9622 Operation Manual Touch down Stage 1 94°C, 5 min 94°C, 30 sec Stage 2 60°C (-0.5°C/Cycle), 30 sec 30 cycles 72°C, 30 sec 94°C, 30 sec Stage 3 45°C, 30 sec 30 cycles 72°C, 30 sec Stage 4 72°C, 7 min Stage 5 4°C, Hold...

  • Page 35: Appendix C: Order Information

    TCST-9622 Operation Manual Appendix C: Order Information TCST-9622 DTI FabSpeed Thermal Cycler with 96-well sample block (230V) Visit https://store.dsstakarabio.com/ for more detailed product information For more information contact directly below; Address: DSS Takara Bio India Pvt Ltd, A-5 Mohan Co-operative, Industrial Estate, Mathura Road,...

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