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Summary of Contents for Four E's Scientific CytoCube
- Page 1 Operating Instructions Portable Cell Counter...
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Page 2: Table Of Contents
Contents Introduction Safety Instructions Cell Counter Quick Start Guide Chapter 1 Introduction 1.1 System Overview 1.2 Function Overview 1.3 Parameters Chapter 2 Operation Instruction 2.1 Installing the Instrument 2.2 Turn On or Off the Instrument 2.3 Focus adjustment 2.4 Diameter Range, Living / Dead Cell Threshold Adjustment 2.5 Cell Counting 2.6 Viewing Result 2.7 Data Export... -
Page 3: Introduction
Introduction Thank You for purchasing Cell Counter. Please read this manual carefully before using the instrument, follow the instructions and procedures, and beware of all safety instructions when using this instrument. Please keep the manual in an easy accessible place for reference. In this manual, "user"... -
Page 4: Safety Instructions
Safety Instructions In order to safely operate automatic portable cell counter, please pay attention to the following safety instructions and operation guidelines: • Place the equipment on a dry, solid and stable table. • Don’t keep equipment in a humid environment. •... -
Page 5: Cell Counter Quick Start Guide
accuracy. • Ensure that the EasyFive counting slide is not contaminated before use. A clean counting slide can guarantee the performance. Cell counter quick start guide 1. Boot Turn on the Laptop /PC power switch and connect the cell counter correctly. 2. -
Page 6: Chapter 1 Introduction
5. Analysis II Adjust the focus until the sample image is clear, click "Count" 6. View Results 2 seconds later, analysis results will appear. Click “Resul” tab to check the detailed data. Chapter 1 Introduction Cell Counter is a portable cell counter that can quickly and accurately detect total cell concentration and cell viability. -
Page 7: System Overview
the current sample being analyzed is sample two, and so on. Cell Counter system can accurately analyze adherent cells, suspended cells, stem cells and insect cells in a concentration range of 1*104~3*107cells/mL. The diameter range is 8~200μm, Accurate cell information, such as cell concentration, cell viability and diameter distribution also can be checked. - Page 8 • Slot indicator The indicator light in front of the cell counter corresponds to slide chamber number. When one light is on means the location is sample one; When the second light turns on, it means the sample being analyzed is sample in slide chamber 2.
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Page 9: Function Overview
1.2. Function Overview The software is divided into the following two parts: login interface (as shown in Figure 2) and main menu interface. FIG. 2 Login interface • Login Interface Connect to computer correctly, then double-click the software icon to open the software. - Page 10 “Log”, “Device”, “Calculator” and “Logout”. Compared with the “super administrator mode”, the “administrator mode” does not have the operation rights to change “Storage” path. • User It is used for laboratory managers to manage the users of this instrument. • Log Record the log history of all users in this instrument.
- Page 11 Figure 3 Main menu interface (super administrator mode) Figure 4 Main menu interface (administrator mode) Figure 4.1 Main menu interface (User mode)
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Page 12: Parameters
1.3. Parameters Table 1 Instrument Instrument Type Portable cell counter specifications Size (W*D*H) 255 x 164 x 97mm Weight 1.35 Kg Concentration Range 1 x 10⁴ - 3 x 10⁷cells/mL Diameter Range 8-200μm Activity Range 0-100% Test Time 2-5s Detection Accuracy ±5% The Optical System Bright Field... -
Page 13: Chapter 2 Operation Guide
Chapter 2 Operation Guide 2.1 Installing the instrument • System components Cell counter, USB power cord, equipment manual, warranty card • Installing System Components Carefully open the cell counter box, remove the packing filler and store it for future using. Check the packing list and contents in the packing case. If any component is missing or damaged, please contact seller. -
Page 14: Focus Adjustment
the main menu interface. (Warning: Make sure the USB cable is properly connected to the equipment and computer before activating, otherwise the system will fail to initialize.) New user need to create a personal account before using the equipment for the first time: Click "User", then click "New User" in the upper right corner, input personal information, and finally click "Submit". -
Page 15: Diameter Range, Living / Dead Cell Threshold Adjustment
(3) Adjust the focusing knob on the upper left side of the instrument clockwise or counterclockwise to adjust the cells in the field of vision until the cytoplasm center is translucent, and the cell membrane is clear, as shown in FIG. 5 and 6. (4) After the focus is right, click “Count”... -
Page 16: Cell Counting
any questions about threshold modification, contact your local agent or manufacturer. You can also Save these parameters for later use by using “Save As”. Such saved parameters can be accessed from the “Cell Parameters” tab under the “Analysis” Menu. Figure 7 Figure 8 An example of correct threshold parameters Example of incorrect threshold parameters... - Page 17 5. Ensure that all equipment for sample preparation such as EasyFive counting slide, pipette tip is stored in a dust-free environment, as dust or particles may affect image quality. 6. Do not touch the counting area of the EasyFive cell counting slide. Hold the edge of the slide, and make sure the EasyFive counter slide is not contaminated before use.
- Page 18 between "Bright Field" or "Trypan Blue" counting mode to preview the unit image (as shown in Figure 9). In the Bright Field mode, only live cells are labeled and the total number of live cells is calculated. The Trypan Blue mode can count the number of live and dead cells, as well as calculate cell viability.
- Page 19 same as “Bright Field” mode. • Cell counting Preparation 1. Take a new EasyFive six-position cell counting slide out of the cover, tear off the upper and lower surface protective films, place the EasyFive six-position cell counting slide on a horizontal clean bench, and load 25µL cell sample into the chamber.
- Page 20 2. Complete counting analysis in one step. Insert the EasyFive cell counting slide horizontally into the slot and click analysis to calculate the cell viability. (Note: Do not touch the optical surface of the EasyFive cell counter. Hold the edge of the counter by hand, otherwise fingerprints will affect the count accuracy.) 3.
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Page 21: Viewing Result
2.6. View Results After counting, results will be displayed directly (as shown in Figure 11), and all test results will be automatically stored in the connected computer, including images and the parameters. • Sample Image Cell Counter takes single photo of each cell sample, and the tagged cells can be observed after counting. -
Page 22: Data Export
FIG. 12 Distribution of cell diameter • Result Report document Click "Result" under the analysis menu (as shown in Figure 11) to view the overall analysis report of sample cells (.pdf file). 2.7. Data Export All sample data counted by the cell counter is stored in the connected computer. - Page 23 • Export the target sample data Click "Result" to find the specific sample data (as shown in Figure 13), check it and export it to the corresponding folder of cell counter for storage, or export it to other storage equipment through USB drive. FIG.
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Page 24: Return To Main Menu & Powering Down
2.9. Return to Main Menu and Powering Down • Return to main screen After completing data analysis, result review, data saving and export, please click the return icon ( ) of the interface to return to the main interface for testing the next sample. •... -
Page 27: Chapter 4 Warranty
Chapter 4 Warranty This instrument is warranted to be free from defects in materials and workmanship under normal use and service, for a period of 12 months from the date of dispatch. The warranty is extended only to the original purchaser. - Page 28 Date：2024.03.04 Version：V1.0...
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