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Caliper Life Sciences IVIS Lumina XR System Manual

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System Manual
IVIS Lumina XR
Real-Time Bioluminescence, Fluorescence & X-Ray
Imaging System
October 2009

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Summary of Contents for Caliper Life Sciences IVIS Lumina XR

  • Page 1 System Manual IVIS Lumina XR Real-Time Bioluminescence, Fluorescence & X-Ray Imaging System October 2009...
  • Page 2 E-mail: tech.support@caliperLS.com www.caliperLS.com IVIS and Living Image are registered trademarks or trademarks of Caliper Life Sciences, Inc. The names of companies and products mentioned herein may be the trademarks of their respective owners. Apple, Macintosh, and QuickTime are registered trademarks of Apple Computer, Inc. Microsoft, PowerPoint and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries.

  • Page 3: Table Of Contents

    5.5 Regulatory Compliance ........17 6 IVIS Lumina XR Components & Specifications ..... 19 6.1 CCD Camera .
  • Page 4 ........33 8.1 Surveying the IVIS Lumina XR for Radiation Leakage ....33 8.2 Maintenance &...
  • Page 5 Contents iii Appendix D Removing & Replacing the Scintillation Plate Holder ... 67 D.1 Removing the Scintillation Plate Holder ......67 D.2 Replacing the Scintillation Plate Holder .
  • Page 6 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 7: Welcome

    The IVIS Lumina XR takes very low light level images that can be saved and displayed for subsequent analysis. In addition to bioluminescent and fluorescent imaging, the IVIS Lumina XR incorporates planar X-ray equipment that enables automated X-ray co- registration to optical images.

  • Page 8: Caliper Technical Support

    43. For instructions on using the Living Image software, please see the Living Image ® Software User’s Manual. If you have questions regarding this manual or the IVIS Lumina XR, please contact Caliper technical support. 1.2 Caliper Technical Support Telephone: 1.877.522.2447 (US)

  • Page 9: Important Safety Instructions

    Observance of safety precautions will help avoid actions that could damage or adversely affect the performance of the IVIS Lumina XR. If the equipment is used in a manner not specified in this manual, the protection provided by the equipment may be impaired.

  • Page 10: Instructions

    IVIS ® Lumina XR Imaging System Manual 2.2 Safety Symbols shows safety symbols that are found on the IVIS Lumina XR and in this manual. Table 2.1 Table 2.1 Safety symbols Symbol Definition Warning: Hazardous voltage Warning: The equipment produces X-rays when energized.

  • Page 11: Chapter 2 | Important Safety Instructions

    Chapter 2 | Important Safety Instructions DO NOT operate the IVIS Lumina XR unless an X-ray safety survey has been performed within the last 12 months. For more information, please contact Caliper technical support. An X-ray safety survey must be performed when the instrument is installed or after it has been moved, unless the instrument was moved on the XWS-260 Workstation cart and no abnormal mechanical shocks occurred.

  • Page 12: Cleaning Or Moving The System Components

    There is sufficient space behind the IVIS Lumina XR equipment. A minimum space of four inches from the flat surface of the rear panel should be provided behind the IVIS Lumina XR to provide unobstructed air flow and access to the main power on/ off switch.

  • Page 13: Power Considerations

    The IVIS Lumina XR is configured for the voltage requirements of the installation locality that was specified at the time of order. If the IVIS Lumina XR is moved to another area, make sure that the same voltage requirements exist.

  • Page 14: Other Equipment

    Do not modify the IVIS Lumina XR in ANY manner by making any kind of hole or aperture in the instrument or removing any component of the radiation shielding.

  • Page 15: Warnings

    Always turn off the switch on the front panel of the light source module and the rear-mounted ON/OFF switch on the IVIS Lumina XR before you connect or disconnect any of these cable connections.

  • Page 16: Eye Safety And Burn Hazard

    See Lamp Replacement, page 3.4 Mechanical Safety The imaging chamber of the IVIS Lumina XR is heavy and weighs 160 lbs. (73 kg). IMPORTANT ALERT! The IVIS Lumina XR may only be moved on the XWS-260 workstation. For...

  • Page 17: Chemical & Biological Safety

    There are no user serviceable components in the lower electronics tray of the IVIS Lumina XR. Do not remove the electronics tray from the IVIS Lumina XR or the cover from the light source module unless you are instructed by and under the supervision of a Caliper technical service representative.
  • Page 18 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 19: Legal Notices

    Trademarks ........14 4.1 Limited Warranty Caliper Life Sciences, Inc. ("Caliper") provides the following limited warranty for each new IVIS Lumina XR ("System") as follows ("Limited Warranty"): i.

  • Page 20: Patents

    4.3 Trademarks IVIS and Living Image are registered trademarks or trademarks of Caliper Life Sciences, Inc. The names of companies and products mentioned herein may be the trademarks of their respective owners. Apple, Macintosh, and QuickTime are registered trademarks of Apple Computer, Inc.

  • Page 21: Ray Safety & Radiation Hazards

    X-ray system. 5.2 Effects of Radiation The IVIS Lumina XR produces ionizing radiation in the energy range from 20 to 40 kilovolts (X-ray). While this energy can be hazardous to the human body, the shielded cabinet protects the user or others in the vicinity from any exposure above background.

  • Page 22: X-Ray Dose Limits

    Refer all servicing to Caliper technical support. 5.3 X-Ray Dose Limits A sample of Caliper Model IVIS Lumina XR has been tested at maximum operating conditions. Caliper has determined the local X-ray dose rate at a distance of 5 cm from the surface of the equipment is less than 1.0 mSv/h.

  • Page 23: Regulatory Compliance

    WARNING! A Caliper employee will conduct a radiation leakage survey and safety tests after the IVIS Lumina XR is serviced by Caliper. Caliper employees are trained in radiation safety. However, check with your local radiation control authority to determine the specific radiation survey requirements at your facility.
  • Page 24 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 25: Ivis Lumina Xr Components & Specifications

    The system is controlled by a pre-configured computer running Living Image software. ® There are no user-serviceable parts in the IVIS Lumina XR. For more details on the fluorescence equipment, see Chapter 10, page Figure 6.1 IVIS Lumina XR imaging system...

  • Page 26: Ccd Camera

    ® Caliper, all warranties that cover this product are void. In addition, the computer included with the IVIS Lumina XR is specifically configured to run all system-related applications. Any modification of existing software or hardware voids all warranties. If you have any questions, please contact Caliper technical support.
  • Page 27 Chapter 6 | IVIS Lumina XR Components & Specifications 21 • X-ray mode • Any combination of bioluminescence, fluorescence, and/or X-ray mode Operating in X-ray mode requires activation with a key-operated selector switch on the imaging chamber front panel. (For more details, see page page 28.)

  • Page 28: X-Ray Scintillation Module

    Figure 7.3, page 29) that controls the electrical power to the full instrument is on the rear of the IVIS Lumina XR. Activation of this switch provides power to the instrument, but does not permit energizing the X-ray source unless the following conditions have been met: 1.

  • Page 29: Key Selector Switch & Lost Keys

    ® of this requirement is to prevent untrained and unauthorized personnel from operating the X-ray functionality of the instrument. The key-operated switch on the IVIS Lumina XR fulfills this requirement when used in conjunction with the user's own written radiation safety procedures.
  • Page 30 IVIS ® Lumina XR Imaging System Manual Figure 6.4 IVIS ® Lumina XR imaging chamber Imaging Chamber Features • Custom zero-background imaging chamber • Eight position removable optical filter emission wheel and a 12-position excitation filter wheel • High-efficiency lens assembly •...

  • Page 31: Optical Filter Wheel

    Office is installed as well as the Living Image software that controls the ® 6.1) IVIS Lumina XR. ® The computer controls the IVIS Lumina XR hardware, including the CCD camera. A printer can be connected to the computer. Computer Features • High speed Windows -based PC ®...

  • Page 32: Computer Specifications

    IVIS ® Lumina XR Imaging System Manual Computer Specifications Computer Description Power requirements 1.0 A at 120 V 0.5 A at 240 V 50-60 Hz Dimensions 15.75” x 17” x 4.75” 40 cm x 44 cm x 12 cm Weight 22 lbs 10 Kg Computer Monitor Specifications...

  • Page 33: Operating The Ivis Lumina Xr

    The IVIS Lumina XR allows you to monitor and record cellular and genetic activity within a living organism, in real time. The imaging system captures, quantifies, and images the light emitted by a sample.
  • Page 34 IVIS ® Lumina XR Imaging System Manual The temperature square is green when the operating temperature is reached. Click the temperature square to display the current temperature. Figure 7.1 IVIS Acquisition Control panel in the Living Image software 8. Allow the system to initialize. —...

  • Page 35: Restarting The System After A Power Outage

    Chapter 7 | Operating the IVIS Lumina XR 29 7.2 Restarting the System After a Power Outage If the IVIS Lumina XR experiences a loss of supply power, turn off the power switch on each component. Do not restart the system until reliable line power has been restored.

  • Page 36: Door Operation

    7.4 Door Operation The IVIS Lumina XR imaging chamber door has custom designed hinges, seals, and a four-point closure mechanism. The door is designed to provide a light-tight seal over numerous opening/closing cycles and should close easily without excessive handle turning resistance.

  • Page 37: Imaging Basics

    Chapter 7 | Operating the IVIS Lumina XR 31 Stage Curtain A stage curtain is attached to the IVIS imaging chamber platform and covers the empty space beneath the platform . The stage curtain serves as a reminder to not place (Figure 7.5)

  • Page 38: System Shut Down Procedure

    (mostly plants), mouse urine, and animal bedding have been found to glow. Use caution when introducing materials into the IVIS Lumina XR. It is advisable to pre- screen all items by imaging them alone, before imaging them with samples under study.

  • Page 39: Care & Maintenance

    Cleaning the Scintillation Plate Window & Holder ....35 8.1 Surveying the IVIS Lumina XR for Radiation Leakage Caliper recommends, and some local government agencies may require, an X-ray leakage safety test be performed under the following conditions: •...

  • Page 40: Cleaning The Ivis Lumina Xr

    IMPORTANT ALERT! Do not spray cleaning solutions in the imaging chamber. Gently wipe the imaging chamber surfaces as described on page Table 8.1 Acceptable cleaning solutions for the IVIS Lumina XR imaging chamber Cleaning Solution Manufacturer ® Cidexplus Solution (3.4% glutaraldehyde)

  • Page 41: Cleaning The Scintillation Plate Window & Holder

    ® Disinfectant Pharmacal Research Laboratories, Inc. NOTE Caliper makes no claims as to the sterility of the IVIS Lumina XR imaging chamber after using the solutions in . Please refer to the manufacturer’s literature for Table 8.1 information as to the applicability of the compound for the organism of interest.
  • Page 42 IVIS ® Lumina XR Imaging System Manual Gas tubing connected Gas tubing connected at Gas manifold gas hose disconnected from the right- at the barbed fitting on the right-hand elbow on hand elbow the stage the gas manifold Figure 8.1 Gas manifold connections 2.
  • Page 43 Chapter 8 | Care & Maintenance 37 Figure 8.3 Apply optical cleaner to a soft, lint-free wipe 4. Starting at the middle of the plate, wipe the window surface using a gentle circular motion to remove dirt or smudges (Figure 8.4) Figure 8.4 Wipe the window surface 5.
  • Page 44 IVIS ® Lumina XR Imaging System Manual Figure 8.5 Support the alcohol-soaked lint-free wipe with a foam paint applicator NOTE If this method does not completely clean the plate holder, it may be necessary to remove the plate holder from the drive mechanism for cleaning. Instructions on how to remove and clean the plate holder are detailed in Appendix D, page...

  • Page 45: Troubleshooting

    CCD camera to maintain the CCD ® at -90° C. If the camera power supply remains on (IVIS Lumina XR box), the system maintains this temperature regardless of whether the Living Image software is open or the computer is turned on.

  • Page 46: Photographic Image Is Unacceptable

    IVIS ® Lumina XR Imaging System Manual 9.2 Photographic Image Is Unacceptable Photograph imaging parameters are automatically controlled and generally produce a good quality photo. If shiny objects are imaged, creating specular reflections, the automatic algorithm may get confused and produce an underexposed image. Also, refer to the Living Image Software Manual for further details on acquiring images.

  • Page 47: No Image Produced

    Figure 9.3 High reflectance hemisphere Wear powderless gloves when handling the hemisphere to prevent surface contamination that may glow when using the IVIS Lumina XR. 9.4 No Image Produced If no image is produced, there may be an error in the Living Image software, a problem with the physical connections to the camera, or a hardware failure.
  • Page 48 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 49: Fluorescence Module

    The Fluorescence Module provides the IVIS Lumina XR with fluorescent imaging capability. The fluorescence equipment can be used for in vitro or in vivo applications. The sensitive range of the IVIS Lumina XR CCD camera sets the wavelength range for fluorescence applications, which is approximately 400-950 nm As with bioluminescent imaging, wavelengths greater than 600 nm are preferred for in vivo fluorescent applications due to lower absorption in tissue.

  • Page 50: Specifications

    XR to enable fan cooling as well as adequate room for fiber optic cable routing. Chemicals Required for Operation No chemicals are required for the operation of the IVIS Lumina XR or the fluorescence equipment. Other user supplied chemicals or materials may be required for your specific biological testing procedures.

  • Page 51: Description & Theory Of Operation

    This light source consists of a 150-watt quartz tungsten halogen lamp with a dichroic reflector. shows the relative spectral radiance output of the lamp/ Figure 10.4 reflector combination and indicates emission throughout the IVIS Lumina XR wavelength range of 400-950 nm.
  • Page 52 The lamp output is delivered to the excitation filter wheel assembly on the (Figure 10.5) back of the IVIS Lumina XR imaging chamber using a 1/4 inch diameter fiber optic bundle. shows a cross section of this excitation filter wheel assembly. Light Figure 10.6...
  • Page 53 Fluorescent emission from the target fluorophore is collected through an emission filter wheel located at the top of the IVIS Lumina XR imaging chamber and then focused into the CCD camera. The emission filter wheel contains eight openings. Users have the ability to choose up to seven emission filters (60 mm diameter), leaving one position open for bioluminescent imaging.
  • Page 54 The use of high quality filters is essential for obtaining good signal-to-background levels (contrast) in fluorescence measurements, particularly in a high sensitivity instrument such as the IVIS Lumina XR. Typical excitation and emission fluorophore spectra are shown , along with idealized excitation and emission filter transmission curves Figure 10.8...
  • Page 55 OD7. Filter passbands for the standard set are listed in Table 10.1 Table 10.1 Standard filter sets, fluorescent dyes, and proteins used with the IVIS Lumina XR. Fluorophore Standard High Resolution...

  • Page 56: Acquiring Fluorescent Images

    IVIS ® Lumina XR Imaging System Manual 10.4 Acquiring Fluorescent Images NOTE This chapter provides a quick start guide to acquiring fluorescent images. See the Living Image® Software User Manual for complete details. IVIS System Control Panel Overview Acquiring fluorescent images using Living Image software is controlled through the IVIS Acquisition Control Panel .

  • Page 57: Troubleshooting

    Chapter 10 | Fluorescence Module 51 3. Place the sample to be imaged in the center of the stage in the imaging chamber and close the door. 4. Select the Fluorescent check box. 5. Make a selection from the Field of View drop-down menu on the left side of the Control Panel.

  • Page 58: Electrical Power

    IVIS ® Lumina XR Imaging System Manual 4. Take a fluorescent image and check for light and fan operation by looking through the fan guard on the rear panel of the light source module. If there is neither, a blown fuse is the probable cause for light loss.
  • Page 59 Chapter 10 | Fluorescence Module 53 NOTE For the software to control lamp functions, the front panel switch on the light source module must be in the ON position. 11. Resume normal operation. Lamp Replacement WARNING WARNING! The following procedure can expose the user to hazardous voltages unless the electrical power to the light source module of the fluorescence equipment is completely eliminated.
  • Page 60 IVIS ® Lumina XR Imaging System Manual 6. Remove the lamp assembly from the light source module by slowly pulling outward. 7. Check the lamp assembly to verify that the lamp and socket are cool before proceeding. 8. Push back on the lamp holder release lever to raise the lamp (L) (Figure 10.12) 9.

  • Page 61: Fluorescence Hardware Replacement Parts List

    WARNING! DO NOT use fluids to clean the exterior (or interior) of the module. Do not allow fluids of any kind to enter the light source module under any circumstances. Sprays and liquids that come into contact with the light source module or the IVIS Lumina XR may result in damage to the system or electrocution.
  • Page 62 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 63: Appendix A Moving The Ivis Lumina Xr With The Xws-260 Workstation

    (Figure A.1) CAUTION CAUTION! Caliper recommends that you do not move an IVIS Lumina XR that is not located on the XWS-260 workstation. If you need to move an imaging system that is not on the workstation, contact Caliper technical support for assistance.

  • Page 64: Moving The Imaging System On The Xws-260 Workstation

    2. Turn on the power surge protection devices. 3. Turn on the computer and monitor. 4. Turn on the IVIS Lumina XR imaging chamber (the power switch is located on the back of the unit) and verify that the other components such as the camera power supply, X-ray controller (both on the back of the unit) and fluorescence lamp are also turned to the On position.
  • Page 65 Chapter A | Moving the IVIS Lumina XR With the XWS-260 Workstation The temperature square is green when the operating temperature is reached. Click the temperature square to display the current temperature. Figure A.2 IVIS Acquisition Control panel in the Living Image software 8.
  • Page 66 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 67: Appendix B Ivis Lumina Xr Options & Accessories

    Appendix B IVIS Lumina XR Options & Accessories Table 2.1 Optional equipment and accessories for the IVIS Lumina Part No. Item 117837 XBP-24 Black Paper (1 tablet of 24 sheets) 118897 XLS-4 Calibrated Light Source 118918 XGI-8 Anesthesia System, 120V...
  • Page 68 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 69: Appendix C Emission Filter Wheel Replacement Instructions

    Installing the Calibration Data ......65 This appendix explains how to replace the emission filter wheel on the following systems: • IVIS Lumina • IVIS Lumina XR • IVIS Kinetic C.1 Removing the Filter Wheel 1. Move the stage to Position C.
  • Page 70 IVIS ® Lumina XR Imaging System Manual 4. Lower the front edge of the illumination plate until it rests on the stage. 5. Remove the three flathead retaining screws using the T-handle tool supplied in the filter kit. NOTE At this point, the filter wheel is only retained by magnets. Wear the cotton gloves provided in the filter wheel kit to avoid putting fingerprints on the filters.

  • Page 71: Replacing The Filter Wheel

    Chapter C | Emission Filter Wheel Replacement Instructions 65 6. Remove the filter wheel from the gear. C.2 Replacing the Filter Wheel 1. Put the filter wheel onto the gear. Fit the two pins on the gear into the slots in the filter wheel. Either pin orientation is acceptable.
  • Page 72 IVIS ® Lumina XR Imaging System Manual [This page intentionally blank.]...

  • Page 73: Appendix D Removing & Replacing The Scintillation Plate Holder

    D.1 Removing the Scintillation Plate Holder 1. Close the Living Image software and turn off the power to the IVIS Lumina XR at the main ® power switch that is located at the rear of the instrument near the power cord.
  • Page 74 IVIS ® Lumina XR Imaging System Manual Figure D.2 Remove the buttonhead screws from the sheet metal cover 4. After the sheet metal cover is removed, rotate the scintillation plate out into the center of the stage by pulling the plate holder forward, and then turning it in a counter- clockwise direction.

  • Page 75: Replacing The Scintillation Plate Holder

    Chapter D | Removing & Replacing the Scintillation Plate Holder 69 D.2 Replacing the Scintillation Plate Holder 1. To re-install the plate holder, orient the scintillation plate mounting disk so that the un- chamfered hole is towards the rear of the imaging chamber and slide the paddle underneath this disk (Figure D.4) Figure D.4 Orientation of the paddle mounting...
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  • Page 77: Index

    Index excitation accessories 61 filter 49 acquiring fluorescent images 50–51 filter transmission curve 48 anesthesia filter wheel 46, 47 gas plumbing 29 fluorophore spectra 48 anesthesia delivery system 30 eye and burn hazard 10 bandgap 48 filter lock 50 bandpass filter 48 filter replacement 55 black paper 31 filter spectra 48...
  • Page 78 IVIS ® Lumina XR Imaging System Manual radiation effects 15 key selector switch 23 radiation hazards 15–17 regulatory compliance 17 replace lamp 44 filter 55 lamp replacement 53–54 fuse 52–53 light block 46 lamp 53–54 Light Source Module 45 replacement parts dimensions 44 fuse 55 weight 44...
  • Page 79 Index 73 subject placement 31 XWS-260 workstation 57–59 system cleaning 34–35 environmental requirements 26 moving with XWS-260 workstation 57–59 restarting after power outage 29 shut down 32 start up 27–28, 58–59 system components Fluorescence Module 45 X-ray system 20–22 technical support 2 temperature 44 measured unequal to demand 39 trademarks 14...
  • Page 80 IVIS ® Lumina XR Imaging System Manual...

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