Spectris Malvern Panalytical Zetasizer Advance Series Basic Manual

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Zetasizer Advance Series

Basic Guide

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Page 1 Zetasizer Advance Series Basic Guide...
Page 3 Zetasizer Advance Series Basic Guide Publication date: Tuesday, March 21, 2023 MAN0593-10-EN...
Page 4: Copyright Notice
Disclaimer Although diligent care has been used to ensure that the information in this material is accurate, nothing herein can be construed to imply any representation or war- ranty as to the accuracy, correctness or completeness of this information and we shall not be liable for errors contained herein or for damages in connection with the use of this material.
Page 5: Table Of Contents
Contents Chapter 1 Introduction 1.1 About the Zetasizer Advance Series 1.2 About this manual 1.3 Cover removal 1.4 Assumed information 1.5 Where to get help Chapter 2 Health and safety 2.1 General safety issues 2.2 General maintenance warnings 2.3 Instrument warnings 2.4 Sample handling warnings 2.5 Fumes Chapter 3 Site requirements...
Page 6 Chapter 4 Hardware overview 4.1 System overview 4.2 Instrument 4.3 Accessory options Chapter 5 Quick start 5.1 Introduction 5.2 Start the system 5.3 Fill cells 5.4 Load cells into the instrument 5.5 Set up a measurement 5.6 Run a method 5.7 Analyze the results Chapter 6 Maintenance 6.1 About maintenance procedures...
Page 7: Chapter 1 Introduction
Chapter 1 Introduction 1.1 About the Zetasizer Advance Series 1.2 About this manual 1.3 Cover removal 1.4 Assumed information 1.5 Where to get help...
Page 8: About The Zetasizer Advance Series
CHAPTER 1 INTRODUCTION 1.1 About the Zetasizer Advance Series The Zetasizer Advance Series instruments provide the ability to measure particle size and zeta potential of particles or molecules in a liquid medium. The Zetasizer Advance Series feature unique technology for measurements over a wide concentration range, and precise tem- perature control to give reproducible, repeatable and accurate measurements.
Page 9: About This Manual
CHAPTER 1 INTRODUCTION Lab Blue Pro Blue Ultra Blue Lab Red Pro Red Ultra Red Zeta measurement Particle concentration pH titration DDLS Fluorescence filter 'Y' indicates feature is available for this product type. 'N' indicates feature is unavailable for this product type. 'O' indicates an optional feature.
Page 10 CHAPTER 1 INTRODUCTION Zetasizer Pro Red ZSU3205 Zetasizer Ultra Red ZSU3305 (previously: ZSU5700) For information about the features available for each Zetasizer Advance Series instrument, refer to section 1.1. 1.2.1 Product documentation structure This manual provides the essential information necessary for ensuring safe and efficient oper- ation of the Zetasizer Advance Series.
Page 11: Cover Removal
CHAPTER 1 INTRODUCTION 1.3 Cover removal Malvern Panalytical personnel (service engineers and representatives) are the only people authorized to perform any service procedures that may require the removal of the covers. WARNING - General hazard Removal of the main covers by unauthorized personnel will void the warranty of the instrument.
Page 12: Where To Get Help
CHAPTER 1 INTRODUCTION 1.4.3 Software option selection Software options are shown in bold and take the form main option>sub option. As an example, Home>Measure size refers to the need to select Home and then choose a Size measurement. 1.5 Where to get help This section gives information on the various channels in place to get help with your Zetasizer system.
Page 13: Chapter 2 Health And Safety
Chapter 2 Health and safety 2.1 General safety issues 2.2 General maintenance warnings 2.3 Instrument warnings 2.4 Sample handling warnings 2.5 Fumes...
Page 14: General Safety Issues
CHAPTER 2 HEALTH AND SAFETY 2.1 General safety issues WARNING - General hazard Use of the system in a manner not specified by Malvern Panalytical may impair the protection provided by the system. 2.1.1 Site requirements The system has specific site requirements that must be met to ensure safe operation of the instrument.
Page 15: Temperature Warnings
CHAPTER 2 HEALTH AND SAFETY 2.1.2 Temperature warnings WARNING - Hot surface Warning triangles on the cuvette lid and thermal cap indicate potentially hazardous temperatures within the cell area. The temperature range of the cell area is 0 °C to 120 °C.
Page 16: Electrical Safety Warnings
CHAPTER 2 HEALTH AND SAFETY 1. CLASS 1 LASER PRODUCT 2. CAUTION - CLASS 3R LASER RADIATION WHEN OPEN. AVOID EXPOSURE TO THE BEAM. Figure 2.1 Laser warning labels Note: The Lab Red, Pro Red and Ultra Red instruments use a class 3B laser. The Lab Blue, Pro Blue and Ultra Blue instruments use a class 3R laser. 2.1.4 Electrical safety warnings Take care when measuring samples not to spill liquid on the system covers.
Page 17 CHAPTER 2 HEALTH AND SAFETY connect the power and clean up before re-applying power to the system. Users who suspect powder or liquid has entered the covers should call a Malvern Panalytical representative to arrange a service call. WARNING - General hazard Never attempt to remove the covers;...
Page 18 CHAPTER 2 HEALTH AND SAFETY WARNING - General hazard Do not use the power cord received with this product on any other products. 2.1.5.3 Power safety information The following notes indicate guidelines to follow when connecting the Malvern Panalytical power supply using single and multiple extension leads, connection via AC Adapters and use of Uninterruptible Power Supplies (UPS).
Page 19: Moving The System
CHAPTER 2 HEALTH AND SAFETY Advice on use of AC adapters WARNING - General hazard Do not use adapter plugs that bypass the grounding feature, or remove the ground- ing feature from the plug or adapter. Place the AC adapter in a ventilated area, such as a desk top or on the floor. The AC adapter may become hot during normal operation of the instrument or accessory.
Page 20: General Maintenance Warnings
CHAPTER 2 HEALTH AND SAFETY 2.1.6.2 Moving the MPT-3 Multi-purpose Titrator and Auto Degasser If it is necessary to move the unit, follow these guidelines: Disconnect the power supply before attempting to move the unit. Disconnect and drain or vent any tubing that carries fluid or compressed air, including sample tubing, before moving the unit.
Page 21: Instrument Warnings
CHAPTER 2 HEALTH AND SAFETY 2.3 Instrument warnings WARNING - General hazard Before cleaning, always disconnect the unit from the power supply and computer and disconnect all electrical cables. Ensure the unit is completely dry before re-apply- ing power. CAUTION After the Zetasizer is powered off, you must wait at least 5 minutes before re-power- ing the system.
Page 22: Fumes
CHAPTER 2 HEALTH AND SAFETY After measuring hazardous samples, clean the system to remove any contaminants before making another measurement. Always label samples for analysis using industry standard labeling, particularly if they are handled by a number of staff or stored for long periods. Clearly label any operator hazard and associated safety precautions that are required for the handling of dangerous mater- ials.
Page 23: Chapter 3 Site Requirements
Chapter 3 Site requirements 3.1 Introduction 3.2 Environmental conditions 3.3 Space required 3.4 Services 3.5 Purge specification 3.6 MPT-3 Multi-purpose Titrator Services 3.7 Computer specification...
Page 24: Introduction
CHAPTER 3 SITE REQUIREMENTS 3.1 Introduction This section outlines the site requirements for the Zetasizer Advance Series. Make sure these requirements are met before the Malvern Panalytical engineer arrives to install and com- mission the system. 3.2 Environmental conditions The site must be: Away from strong light sources (such as windows) Away from strong heat sources (such as radiators) Well ventilated (for noxious samples)
Page 25: Space Required
CHAPTER 3 SITE REQUIREMENTS Condition Requirement Storage conditions -20 °C to 50 ° C (-4 °F to 122 °F) Humidity Maximum humidity 80% for temperatures up to 31 °C, decreasing lin- early to 50% relative humidity at 40 °C. Altitude Up to 2000 m Mains supply voltage fluc- Up to ±...
Page 26: Services
CHAPTER 3 SITE REQUIREMENTS Component Width Depth Height Auto Degasser 75 mm 250 mm 130 mm Computer and printer See manufacturer's documentation The following diagram shows the minimum recommended space required for a typical system. 75mm 1 5 0 1 5 0 Figure 3.1 Space required 3.4 Services This section describes the services required.
Page 27: Power Specification
CHAPTER 3 SITE REQUIREMENTS Table 3.4 Power sockets required for each system unit Component Power Sockets Zetasizer MPT-3 Multi-purpose Titrator Auto Degasser Computer and printer 1 or more. Refer to manufacturer's documentation. 3.4.2 Power specification The mains power supply must be clean and filtered. If necessary, use an Uninterruptible Power Supply (UPS) to remove any spikes or noise.
Page 28: Purge Specification
CHAPTER 3 SITE REQUIREMENTS class 3B laser radiation. Malvern Panalytical therefore recommends that the administrative controls recommendations of the Laser Safety Regulations (ISO 60825-1:2014) are imple- mented. 3.5 Purge specification If measuring samples at low temperatures there is a risk of condensation occurring on the cell; this occurs when the measurement temperature is less than the dew point of the ambient air surrounding the cell being measured.
Page 29: Mpt-3 Multi-Purpose Titrator Services
CHAPTER 3 SITE REQUIREMENTS The flow rate will be preset at 1.5 L/min at 1 bar. The purge must be running prior to demanding a cell temperature below the dew point. Failure to do so may allow condensation to form and removal of condensation is difficult, even with the purge running.
Page 30: Computer Specification
CHAPTER 3 SITE REQUIREMENTS 3.7 Computer specification Contact Malvern Panalytical Support or refer to the website for the recommended computer specification. This is also provided in the Software Update Notification.
Page 31: Chapter 4 Hardware Overview
Chapter 4 Hardware overview 4.1 System overview 4.2 Instrument 4.3 Accessory options...
Page 32: System Overview
CHAPTER 4 HARDWARE OVERVIEW 4.1 System overview Note: The Zetasizer is initially commissioned by Malvern Panalytical trained personnel. This section describes how to reinstall the system, typically required when moving the instrument or changing the computer. The following image shows a typical Zetasizer instrument and its components. A cell is filled with the sample and loaded into the cell area on top of the instrument.
Page 33: Instrument
CHAPTER 4 HARDWARE OVERVIEW 4.2 Instrument The following image and corresponding table describes the components in the Zetasizer instrument. 1. Instrument 2. Cell access button - press to open the cell chamber lid 3. Status indicator light - see below for more information 4.
Page 34: Rear Panel
CHAPTER 4 HARDWARE OVERVIEW Table 4.1 Status indicator colors and meanings Indicator color and state Function Amber - steady Shows the start-up routine is running. Amber - flashing every 5 s Shows that the instrument is at “standby”. The instrument is functioning correctly but is either not connected to the computer or the software has not been started.
Page 35 CHAPTER 4 HARDWARE OVERVIEW 1. Power switch 5. Ethernet connection (diagnostic) 2. Power input socket - from external DC power supply 6. USB connection (diagnostic) 3. Purge connection 7. Laser warning label 4. USB connection to computer 8. Cooling fan Figure 4.3 Instrument rear panel 4.2.3 Cell chamber The cell chamber is where all cells are inserted to undertake a measurement.
Page 36 CHAPTER 4 HARDWARE OVERVIEW WARNING - Hot surface The system is capable of heating the cell to high temperatures. Take care when removing the cells if a measurement has been made at high temperatures. Allow the cell chamber to cool before removing the cell. Note: If the lid is opened with the cell area temperature above 50 °C the instrument beeps three times every few seconds to warn of high temperatures.
Page 37 CHAPTER 4 HARDWARE OVERVIEW 1. Cell chamber lid 5. Thermal contact plates holder - used with DTS1070 cells 2. Cell basin 6. Thermal cap (storage area) 3. Cell holder - containing cell clamp, electrodes and drain port 7. Tubing channel 4.
Page 38 CHAPTER 4 HARDWARE OVERVIEW Component Description Electrode voltage interlock - turns off any voltage to the cell electrodes. To close the lid, push it down until it locks. No measurements can be made unless the lid is fully closed. Cell basin [2] The cell basin is made of an insulating material which provides protection from the heated cell holder and in conjunction with the thermal cap gives temperature stability when heating and cooling the sample.
Page 39 CHAPTER 4 HARDWARE OVERVIEW Component Description Thermal cap [6] The thermal cap gives increased temperature stability for size measurements when heating and cooling the sample. This is important when measuring at either end of the operational temperature range. Place the thermal cap over the cell. WARNING - General hazard With the thermal cap removed both the metal lining of the cap and the top of the cell holder will be exposed.
Page 40: Accessory Options
CHAPTER 4 HARDWARE OVERVIEW 4.3 Accessory options A range of accessories and options, listed below, are available for more advanced meas- urement strategies. The specifications for each product are provided in the Zetasizer Advance Series Accessories Guide . Table 4.3 List of Zetasizer accessories Option Part No.
Page 41 CHAPTER 4 HARDWARE OVERVIEW Option Part No. Description Low volume disposable siz- ZSU1002 This cell is used for low volume size meas- ing cell urements. It can contain a measurement volume as small as 3 μl. It offers extended upper particle size range without the need to modify your sample dispersion conditions.
Page 43: Chapter 5 Quick Start
Chapter 5 Quick start 5.1 Introduction 5.2 Start the system 5.3 Fill cells 5.4 Load cells into the instrument 5.5 Set up a measurement 5.6 Run a method 5.7 Analyze the results...
Page 44: Introduction
CHAPTER 5 QUICK START 5.1 Introduction This section describes the full process of making a measurement, from turning on the system through to analyzing the results. The example given is that of running a size measurement. Zeta potential measurements follow broadly the same principles. Note: This section does not cover how to run Titration or Particle Concentration meas- urements.
Page 45: Fill Cells
CHAPTER 5 QUICK START Figure 5.1 ZS XPLORER desktop icon 4. The initialization routine will then begin. When the status indicator shows steady green, the instrument has completed its initialization procedure. Note: Allow your Zetasizer to thermally stabilise for 30-minutes before use. If the instrument has been stored outside its nominal operating temperature range (10-35°C) a longer stabilization time maybe necessary.
Page 46 CHAPTER 5 QUICK START 1. Flush the cell with ethanol to facilitate wetting. 2. Fill one of the syringes with the DI water and place in one of the sample ports on the cell, the empty syringe is placed into the other port. 3.
Page 47 CHAPTER 5 QUICK START 1. Invert the cell. 2. Using a syringe or pipette, slowly inject your sample into the cell, filling to just over halfway. 3. Check that no air bubbles are trapped in the cell. If there are bubbles, tap the cell gently to dislodge them.
Page 48 CHAPTER 5 QUICK START 3. Make sure the sample depth is between 10 mm and 15 mm by placing the cuvette against the diagram on the inside of the cell area lid, as shown: Max: 15mm Min: 10mm Figure 5.4 Correct sample depth 4.
Page 49: Load Cells Into The Instrument
CHAPTER 5 QUICK START Note: Most cuvettes have a triangle or spot mark, as shown below. This mark should face towards the front of the instrument. Figure 5.6 Front-facing cuvette 5.4 Load cells into the instrument This section gives information about how to load cells into the instrument correctly. Refer to section 4.2.3 for more information about the cell chamber.
Page 50 CHAPTER 5 QUICK START 5.4.1 Size cells Insert a cuvette into the instrument in the direction shown in the following illustration: Figure 5.7 Correct cell insertion - Size cell...
Page 51 CHAPTER 5 QUICK START 5.4.2 Zeta cells Insert a folded capillary cell in the direction shown in the following illustration: Figure 5.8 Correct cell insertion - Zeta cell Note: The Malvern hills logo on the folded capillary cell should face the front of the instru- ment when inserted.
Page 52: Set Up A Measurement
CHAPTER 5 QUICK START 5.5 Set up a measurement The steps required to set up a measurement are as follows: Create a new Method. Specify the individual Step or Group settings. 5.5.1 About methods To run a measurement you must create a Method. The Method builder allows you to create methods.
Page 53: Create A Method
CHAPTER 5 QUICK START 5.5.2 Create a method Creating a size or zeta method is fundamentally the same process, but the types of meas- urement settings for each will differ. Note: This section gives the example of running a size measurement - for more detailed information, including details on how to run pH Titration or Particle Concentration measurements, refer to the Help system or the Zetasizer Advance Series User Guide .
Page 54 CHAPTER 5 QUICK START Figure 5.11 Selecting a cell type 4. Select the appropriate material and dispersant for your sample. Or, to add your own, refer to the User Guide . 5. Select an existing project to save the measurement results in, or create a new project. Note: The data is not necessarily saved to the open project within the Analyze view.
Page 55 CHAPTER 5 QUICK START Figure 5.12 Selecting a step from the side panel Note: Some steps may be grayed out depending on your chosen settings. For example, the cell selected in the image above cannot be used for zeta potential measurements, so the zeta measurement step is unavailable.
Page 56 CHAPTER 5 QUICK START Figure 5.13 Expanded Properties window 5.5.2.1 Setting the step run order Steps run in the order they are shown in the method, from top to bottom. Reorder steps by clicking on the gripper circled in the following image, and dragging them up or down as required: Figure 5.14 Reordering steps...
Page 57 CHAPTER 5 QUICK START 5.5.2.2 Repeating steps or groups Steps can be specified to repeat as many times as required, as can groups. Set the number of repeats by clicking on the repeat icon on the step. In this example the step will run three times. Figure 5.15 Repeating a step 3 times Note: If you repeat a zeta measurement three times, the system will only optimize once.
Page 58: Run A Method
CHAPTER 5 QUICK START 5.6 Run a method This section guides you through the process of running a method. 1. After setting up a method (refer to section 5.5), click Start method at the top of the win- dow: Figure 5.16 Running a method 2.
Page 59 CHAPTER 5 QUICK START Tip: You can carry on working in other areas of the software during the measurement - it is not necessary to observe the measurement taking place. Note: Abort the measurement at any time by clicking one of the red Stop buttons. The sys- tem will ask for confirmation before stopping the measurement.
Page 60 CHAPTER 5 QUICK START Figure 5.19 Completed measurements Tip: The step of the method which is currently running is shown by a flashing icon as in Sample 2 above, where a Particle concentration measurement step is in progress. Tip: Clicking Clear list will remove the sample information from the Method list window. Clicking on a measurement in this window switches between their respective meth- ods.
Page 61: Analyze The Results
CHAPTER 5 QUICK START 5.7 Analyze the results This section gives information on how to analyze the results once you have run a meas- urement. 1. Select the Analyze tab from the top menu. 2. Select a measurement from the Record selector on the left. 3.
Page 62 CHAPTER 5 QUICK START Tip: To customize the workspace layout, click in the top right corner and select a layout from the pop-up menu. 5.7.1 Record selector The Record selector on the left of the Analyze window shows a table of results. From this table, results can be sorted by parameter values, edited, or selected for viewing in the work- space.
Page 63 CHAPTER 5 QUICK START Figure 5.22 Changing the parameters in the table The data shown in the Record selector depends on the project you have selected. Click the Explorer arrow icon to show the Project Explorer. Figure 5.23 Switching between projects Click on the required project to display it in the Record selector .
Page 64 CHAPTER 5 QUICK START Note: Each Project has its own table. You can choose which project your result will appear in when setting up the measurement.
Page 65: Chapter 6 Maintenance
Chapter 6 Maintenance 6.1 About maintenance procedures 6.2 Clean cells and accessories 6.3 Clean the covers...
Page 66: About Maintenance Procedures
CHAPTER 6 MAINTENANCE 6.1 About maintenance procedures Note: Maintenance procedures and details of consumable parts and kits for any accessories are provided in the Zetasizer Advance Accessories Guide . An operator can perform all maintenance procedures outlined in this section. 6.2 Clean cells and accessories Clean cells thoroughly between measurements, especially between different types of sample.
Page 67: Appendix A Specifications
Appendix A Specifications A.1 Specifications A.2 Chemical compatibility...
Page 68: Specifications
Appendix A Specifications A.1 Specifications The following table lists the specifications of the Zetasizer. All specifications are correct at time of publication, but may be subject to alteration. Table A.1 Zetasizer specifications Size measurement specifications Parameter Specification Range (maximum diameter) 0.3 nm - 10 μm* (Pro) Γ...
Page 69 Appendix A Specifications 173° (water as sample dispersant), 90° and 13° (Ultra) Dynamic light scattering (NIBS ® ), Multi angle dynamic light Measurement technique scattering (MADLS®), Dynamic light scattering (13/90/173 degrees) Zeta potential measurement specifications Parameter Specification Size range suitable for measurement Minimum 3.8 nm, maximum 100 µm†...
Page 70 Appendix A Specifications Laser specifications Model Specification Zetasizer Blue Label Systems Type: HeNe gas laser Max. output power: 4 mW Beam diameter: 0.63 mm (1/e²) Beam divergence: 1.5 mrad Beam wavelength: 632.8 nm Attenuation, transmission 100% to 0.0003% Zetasizer Red Label Systems Type: HeNe gas laser Max.
Page 71 Appendix A Specifications General specifications Parameter Specification Detector Avalanche photodiode QE >50% at 633 nm (Lab, Pro, Ultra) Condensation control Purge facility using dry air Temperature control range 0 ºC to 120 ºC** Compatible cell temperatures 0 °C - 120 °C - Glass and quartz cells 0 °C - 70 °C - Low volume disposable sizing cell 0 °C - 70 °C - Folded capillary cell, plastic disposable cells 0 °C - 70 °C - Dip cell...
Page 72: Chemical Compatibility
Appendix A Specifications ‡ Using ZSU1002 low volume disposable sizing cell Γ Indicative data available for particle sizes greater than 10 µm when using Extended size range analysis MADLS ® Multi Angle Dynamic Light Scattering A.2 Chemical compatibility Components of the Zetasizer that may come into contact with the sample are manufactured from materials that are considered to give the widest protection to stop degradation.
Page 73 Appendix A Specifications Component Materials Electrodes Gold plated beryllium/copper Cell holder Aluminum (anodized) A.2.2 Cells and cuvettes Refer to the descriptions in the Zetasizer Accessories Guide for the materials used for each of the available cells.
Page 75: Appendix B Regulatory
Appendix B Regulatory B.1 UKCA Declaration of Conformity B.2 EU Declaration of Conformity B.3 Canadian Regulatory Information (Canada only) B.4 FCC Notice (US only) B.5 Disposal of Electrical & Electronic Equipment...
Page 76: Ukca Declaration Of Conformity
Appendix B Regulatory B.1 UKCA Declaration of Conformity The UKCA badge on this product, where fitted, signifies conformance to the relevant UK reg- ulations - consult the Declaration of Conformity certificate for the product for more inform- ation. B.2 EU Declaration of Conformity The CE badge on this product signifies conformance to the relevant European Directives - con- sult the Declaration of Conformity certificate for the product for more information.
Page 77: Disposal Of Electrical & Electronic Equipment
Appendix B Regulatory The device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: 1. This device may not cause harmful interference, and 2. This device must accept any interference received, including interference that may cause undesired operation.
Page 78 Appendix B Regulatory This symbol on the product or on its packaging indicates that when the last user wishes to discard this product it must not be treated as general waste. Instead it shall be handed over to the appropriate facil- ity for the recovery and recycling of electrical and electronic equip- ment.
Page 80 Malvern Panalytical Ltd. Malvern Panalytical B.V. Grovewood Road, Malvern, Lelyweg 1, Worcestershire, WR14 1XZ, 7602 EA Almelo, United Kingdom The Netherlands Tel. +44 1684 892 456 Tel. +31 546 534 444 Fax. +44 1684 892 789 Fax. +31 546 534 598 MAN0593-10-EN www.malvernpanalytical.com...

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