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CYBIO -FeliX Head R Operating Manual
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CYBIO -FeliX Head R Operating Manual

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Operating Manual
Pipetting Head for CyBio FeliX

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Summary of Contents for CYBIO CyBio-FeliX Head R

  • Page 1 Operating Manual Pipetting Head for CyBio FeliX...
  • Page 3 Document type Translation of Original User Manual version Serial number Refer to product nameplate Registered trademarks: CyBio Analytik Jena GmbH+Co. KG, Germany Title to all other trademarks or brands which are referenced in this User Manual belongs to their legal owners.
  • Page 5 Einbauerklärung (inhaltliche Wiedergabe) Declaration of Incorporation (content reproduction) Name und Anschrift des Herstellers: Name and address of the manufacturer: Analytik Jena GmbH+Co.KG Konrad-Zuse-Straße 1 D-07745 Jena Hiermit erklären wir, dass das nachstehend beschriebene Herewith we declare, that the partly completed product described unvollständige Produkt below Leicht-Pipettierkopf...
  • Page 6 2024...

  • Page 7: Table Of Contents

    Warranty & Liability ....................6 Scope of Delivery ....................6 Technical Specifications 7 CHOICE Pipetting Head ..................8 CyBio-FeliX Head R 384/60 μl ................9 CyBio-FeliX Head R 96/60 μl ................10 CyBio-FeliX Head R 96/250 μl ................11 CyBio-FeliX Head R 96/1000 μl ................12 CyBio-FeliX Head R 384/25 μl ................13...
  • Page 8 4.6.2 Aspiration Without Additional Stroke..............36 Packaging, Transportation & Storage 37 Safety Notes ......................37 Description ......................37 5.2.1 Packaging......................37 5.2.2 Storage ........................37 Routine Start-Up Procedure 39 Safety Notes ......................39 6.1.1 Energy Supplies ....................40 Initial Start-Up & Configuration ................40 Function Tests .......................40 6.3.1 Pipetting Head Precision Test ................

  • Page 9: General Information

    Note This User Manual informs about the setup and function of the Pipetting Head for the CyBio-FeliX from Analytik Jena GmbH+Co. KG. This Manual further contains instructions and advice about general product care and the Manufacturer’s defined scope of maintenance work items.

  • Page 10: Conventions

    General Information Conventions 1.3.1 Textual Markups Work instructions involving a timed sequence are numbered and merged to action units to specify related results. Enumeration involving no timed sequence is shown as a bullet-style list, sub-level numbering or as a dash-style list. Safety notes are marked by a pictogram and a signal word (→...

  • Page 11: Warning Signs

    General Information 1.3.2 Warning Signs WARNING Indicates a potentially hazardous situation. May result in death or serious injury (crippling) if not avoided. CAUTION Dangerous situation! Potential consequences: light or moderate physical injury. NOTE Dangerous situation! Potential consequences: material damage. Useful application advice, no potential danger involved. Note regarding environmental protection.

  • Page 12: Intended Purpose

    General Information Intended Purpose This pipetting head is intended for use in the CyBio-FeliX (flexible pipetting platform for fully automated single-channel or multiple channel liquid handling). Its range of capabilities is restricted by the scope of integrated functional software and firmware, as well as the scope of delivery.
  • Page 13 General Information The definition for non-conforming use includes:  operating the product in medical laboratories of a non-research profile  working with explosive substances  working in an explosive atmosphere – operation in a potentially explosive zone is prohibited Operation involving dangerous substances will be at the sole responsi- bility of the user! This shall include compliance with all valid safety requirements for the protection of persons and material goods during work with radioactive, infectious, poisonous, corro-...

  • Page 14: Warranty & Liability

    General Information Warranty & Liability The period of warranty and scope of liabilities will be as stipulated under binding law and provided for in the General Terms of Business of Analytik Jena GmbH+Co. KG. Warranty will be limited to repair services or replacement of damaged parts. It will exclude consequential damage of any kind.

  • Page 15: Technical Specifications

    Technical Specifications Technical Specifications 2024...

  • Page 16: Choice Pipetting Head

    Precision in the volume range Variation coefficient 100 – 1000 μl ≤ 1% Pipette tip types CyBio Tips (accessories; → „Accessories & Spare Parts” on page 59) Specifications regarding selectable microplates SBS-format 96, 384, Tubes 0.2 – 2 ml Operating data Line voltage +24 V DC (±5%) / 3 A...

  • Page 17: Cybio-Felix Head R 384/60 Μl

    Precision in the volume range Variation coefficient 5 – 60 μl ≤ 1% Pipette tip types CyBio Tips (accessories; → „Accessories & Spare Parts” on page 59) Specifications regarding selectable microplates SBS-Format 384, Tubes 0.2 – 2 ml (LH-adapter) Operating data Line voltage +24 V DC (±5%) / 3 A...

  • Page 18: Cybio-Felix Head R 96/60 Μl

    Precision in the volume range of Variation coefficient 5 – 60 μl ≤ 1% Pipette tip types CyBio Tips (accessories; → „Accessories & Spare Parts” on page 59) Specifications regarding selectable microplates SBS-Format 96, 384, Tubes 0.2 – 2 ml (LH-adapter)

  • Page 19: Cybio-Felix Head R 96/250 Μl

    Precision in the volume range of Variation coefficient 25 – 250 μl ≤ 1% Pipette tip types CyBio Tips (accessories; → „Accessories & Spare Parts” on page 59) Specifications regarding selectable microplates SBS-Format 96, 384, Tubes 0.2 – 2 ml (LH-adapter) Operating data Line voltage +24 V DC (±5%) / 3 A...

  • Page 20: Cybio-Felix Head R 96/1000 Μl

    ≤ 2% Precision in the volume range of Variation coefficient >100 – 1000 μl ≤ 1% Pipette tip types CyBio Tips (accessories; → „Accessories & Spare Parts” on page 59) Specifications regarding selectable microplates SBS-Format Operating data Line voltage +24 V DC (±5%) / 3 A...

  • Page 21: Cybio-Felix Head R 384/25 Μl

    ≤ 2% Precision in the volume range Variation coefficient 5 – 25 μl ≤ 1% Pipette tip types CyBio Tips (accessories; → „Accessories & Spare Parts” on page 59) Specifications regarding selectable microplates SBS-Format Operating data Line voltage +24 V DC (±5%) / 3 A...
  • Page 22 Technical Specifications 2024...

  • Page 23: Safety Notes

    Safety Notes Safety Notes General You should carefully read this chapter. Follow all safety notes that precede described action in the various chapters of this Manual, as well as any message or advisory prompt that may be displayed on the monitor screen by control and evaluation soft- ware tools.

  • Page 24: Standards & Guidelines

    Safety Notes Standards & Guidelines The Pipetting Head has been built to meet currently valid rules of technology and generally established requirements on safety engineering. The product and its components have been designed in accordance with basic safety and health requirements under applicable laws, standards and guideline regulations. All specifications relating to safety reference the European Union regulations in their latest binding revisions.

  • Page 25: Safety Labeling

    Safety Notes Safety Labeling Affixed warning notes and safety symbols are an integral part of the Pipetting Head and must be followed under any circumstances! Check warning labels and safety symbols for intactness and complete- ness before you begin any kind of start-up action. Do not proceed to start-up action if you have identified a missing or damaged warning note or safety symbol! Damaged or missing warning notes or safety symbols may lead to faulty action with...

  • Page 26: Mechanical Danger Zones - Notice

    Safety Notes Mechanical Danger Zones – Notice NOTE Mechanical motion of the Pipetting Head as an integral part of a host system may pose potential hazards to personnel. Always keep your hands/fingers clear of the mechanical motion range as the Pipetting Head (with pipette tips) performs vertical down-motion.

  • Page 27: Requirements For Operating Personnel

    Safety Notes Requirements for Operating Personnel  The product may not be started up, operated or maintained other than by duly Requirements under trained expert personnel having received instructions on operational safety. Such specific product training will also include familiarization with the contents of this Manual and man- aspects: uals of related product components or additional equipment units as may be appro- priate from case to case.

  • Page 28: Safety Notes For Operation

    Safety Notes Safety Notes for Operation 3.8.1 General  Operating personnel are obliged to convince themselves of the proper technical condition of the product and its components, including that of safety devices, before they can proceed to action for powering up. Notably, this requirement applies fol- lowing a change in, an extension to or a repair of the product.

  • Page 29: Fundamental Maintenance & Care Rules

    (as a mandatory component of accident prevention measures): Chapter → “Safety Labeling” on page 17  operating manual CyBio-FeliX, chapter "Safety labeling" The product owner will be solely responsible for compliance with all safety requirements in place for the protection of persons and material goods during work involving radioactive, infectious, toxic, etching, combustible and other dangerous substances.

  • Page 30: Chemical Resistance

    Safety Notes 3.8.6Chemical resistance The manufacturer cannot be held liable if the operator of the device ana- lyzes aggressive substances which may affect the durability of the components. Caution when handling bases, acids and organic solutions. These substances may negatively affect the useful life of the device. Only use substances compatible with the materials listed.
  • Page 31 Safety Notes The components listed in the overview (→ Table 3, “Components”, on page 22), the basic unit CyBio-FeliX (including the corresponding pipetting heads) and any accesso- ries are not resistant to the following substances: Table 4: Substances Substances Hydrofluoric acid (HF / fluoric acid)
  • Page 32 Safety Notes Approved for wipe disinfection (→ Table 5, “Disinfection methods / disin- Disinfection and fectants”, on page 23): chemical resistance CyBio-FeliX Basic Units (OL5015-2X-1XX / OL5015-2X-5XX) CyBio-FeliX Heads (OL3316-14-X5X) Cover magazine (transport protection; OL3316-11-200) BioShake 3000 series (QINSTRUMENTS-2016-0XXX) BioShake wiring Mounting Kit –...

  • Page 33: Rules Of Conduct In Cases Of Emergency

    Safety Notes Rules of Conduct in Cases of Emergency Use the product’s main power switch (On/Off switch) immediately on noticing a dangerous situation and/or disconnect the power plug from the line socket in this case! Since prompt reaction can save lives in a situation of danger, make sure that the following requirements are met: Operating personnel must be aware of the locations and the proper han- dling of safety devices, accident and hazard alarms, as well as first-aid kits...
  • Page 34 Safety Notes 2024...

  • Page 35: Technical Description

    → “Nameplate” on page 28 → “Safety Labeling” on page 17 Pipetting Head – lower part (tip holder) ® → “CyBio -FeliX Head R” → page 29 → “CHOICE Head” on page 29 1 Figure shows a version Head R...

  • Page 36: Nameplate

     Manufacturer’s data  Product information (type designation, tradename)  Identification code (model & serial number)  Year of manufacture Versions Summary Table Head R CHOICE Head Tip change (automatic) CyBio RoboTipTrays – LH-adapter – (OL3316...) LH-adapter – (OL3317...) 2024...

  • Page 37: Components

    4.3.1 Pipetting Heads This Pipetting Head is equipped with a mechanism to allow the automated mounting of CyBio-FeliX Pipetting CyBio RoboTipTrays and liquid handling adapters. Head R Liquid handling adapters are intended for single-channel column-by-column or line-by- line pipetting. A liquid handling adapter provides support bases (cone(s)) for mounting the pipette tips onto.
  • Page 38 A CHOICE adapter provides support bases (cone(s)) for mounting the pipette tips onto. Fig. 5: CHOICE Head (bottom view) Gripper claws Sealing mat Frame for sealing mat CHOICE adapter Fig. 6: CHOICE adapter (12 channels) For further information about accessories, you should consult chapter 11 (CyBio-FeliX Basic Unit). 2024...

  • Page 39: Head Mount Dovetail Guide

    Technical Description 4.3.2 Head Mount Dovetail Guide Fig. 7: Dovetail guide at the Pipetting Head 4.3.3 Electrical Connection Fig. 8: Electrical connection (connector terminal) 2024...

  • Page 40: Mode Of Operation

    The time required to reach this state depends - among other factors - on the properties of the liquid being handled. For further information, please refer to the CyBio-FeliX Manual. 4.4.1 Pipetting The term pipetting means that a defined volume of liquid is aspirated (with/without additional stroke) from a source to be subsequently dispensed into a destination or a waste bin (with/without blow-out function).

  • Page 41: Dispensing

    Operating Modes Different operating modes are available. For further information, please refer to the CyBio-FeliX Manual. Volume Cycle Liquid volumes are aspirated and discharged by piston motion. The mechanical design of piston suspension implies a certain freeplay (backlash). This means that after each reversal of the direction of piston motion, the piston drive travels a certain path length without producing actual piston motion, i.e.

  • Page 42: Aspiration With Additional Stroke

    Technical Description path length corresponds to the amount of freeplay. In order to prevent pipetting errors, a volume cycle is organized in a manner to ensure that the piston reversal point is located outside of the actual pipetting range (motion to zero-position, aspiration with additional stroke) or the reversal zone will be compensated by appropriate motion, respectively (discharge with blow-out) 4.6.1...
  • Page 43 Technical Description blow-out (5) function (→ “Residual Discharge (Blow-Out)” on page 33). A volume cycle is completed on "Motion to zero-position". The "Move pistons into zero-position" (6) command triggers upward piston motion into the starting position, overcoming the lower amount of freeplay. This process step must be performed without liquid (tips cannot make contact with liquid).

  • Page 44: Aspiration Without Additional Stroke

    Technical Description 4.6.2 Aspiration Without Additional Stroke Fig. 10: Aspiration without additional stroke Zero-position A1=D1:Nominal pipetting volume Aspiration D2:Air Upper freeplay Discharge with additional stroke Motion to zero-position Where liquid is taken up without additional stroke motion, it must be discharged in a single step in order to achieve precise results.

  • Page 45: Packaging, Transportation & Storage

    Packaging, Transportation & Storage Packaging, Transportation & Storage Safety Notes NOTE Environmental influences, mechanical shocks or formation of con- densed water may destroy individual product components! Adequate precautions should be taken to protect all components from environmental impacts, mechanical shock or formation of condensed water during transportation or shipment! Temporary open-air stor- age of the product is forbidden! NOTE...
  • Page 46 Packaging, Transportation & Storage packing case. Climatic requirements on facilities for the Pipetting Head storage are as follows:  Temperature range: -10 °C to +50 °C  Permissible relative air humidity: ≤ 85 % at 30 °C, no formation of condensate 2024...

  • Page 47: Routine Start-Up Procedure

    Routine Start-Up Procedure Routine Start-Up Procedure Safety Notes The requirements regarding operating climate are as follows:  Temperature range:+15 °C to 35 °C  Permissible relative air humidity: ≤ 75 % at 35 °C, no formation of condensate The atmosphere inside the operating room should be dust-free to a maximum possible degree, free from drafts and free from etching vapours.

  • Page 48: Energy Supplies

    Routine Start-Up Procedure 6.1.1 Energy Supplies Applies in conjunction with the instrument CyBio-FeliX: WARNING In the event of a break in protective conductor wiring, there is life- threaten ding danger due to electrical shock! Insert the main power plug only into a mains socket with a grounded...

  • Page 49: Pipetting Head Precision Test

    Routine Start-Up Procedure 6.3.1 Pipetting Head Precision Test Testing for variation coefficient CV (percentage standard deviation) is performed using a 96-well or 384-well microplate with transparent flat bottom and dye solution. A suitable vertical photometer serves as the measuring tool. Its own precision must have been tested/verified and documented prior to measurement.
  • Page 50 Routine Start-Up Procedure Table 6: Aspiration and test volume Head type Prefill Test p-nitrophenol dye volume volume solution 96/60 μl 190 μl &197 μl 10 μl & 3 μl 2,4 mM for 10 μl MP 96 12 mM for 3 μl 96/250 μl 150 μl &190 μl 50 μl &...

  • Page 51: Accuracy Test

    Routine Start-Up Procedure 6.3.2 Accuracy Test Accuracy defines the degree of agreement between a measured dispensed volume (mean value of all measurements in a 96-well or 384-well microplate) and a specified (target) volume.  Laboratory scales with resolution not less than 1mg Material / prepara- tion: ...

  • Page 52: Leak Test

    Use setting screws as necessary to adjust horizontal position.  Remove tips, CyBio TipTray, CyBio RoboTipTray and LH-adapter as may be necessary from case to case.  Inspect O-rings thoroughly for cracks or sedimentation with the help of a suitable light source.

  • Page 53: Operation

    Operation Carefully read the following information: Chapter 7 of the CyBio-FeliX user manual  Chapter → “Changing the pipetting head” on page 46 1 If not included, this can be obtained from Analytik Jena GmbH+Co. KG. 2024...

  • Page 54: 7.3Changing The Pipetting Head

    Operation 7.3Changing the pipetting head The pipetting head can be inserted / replaced while the device is switched on or off. The seating of the head (X axis) should be in central position (can be moved manually). The seating of the head (Z axis) should not be in its topmost posi- tion.

  • Page 55: Mounting The Pipetting Head

    Operation 7.3.1 Mounting the Pipetting Head Make sure that the head mount is in mid-pipettor position, not in uppermost vertical position. Swing clamping lever around into front position. Fig. 11: Head mount (with clamping lever in front position) Mount pipetting head. Fig.
  • Page 56 Operation Raise blind to open Swing clamping lever into front position. Fig. 14: Pipetting head (fixed) Take the pipetting head off. Fig. 15: Removal of pipetting head Fig. 16: Head mount (with clamping lever in front position) Check for proper fixation of the head and close the blind 1 Does not apply for enclosure-less model (for example 30-5015-500-24).

  • Page 57: "What To Do If

     Make sure that connected assemblies are firmly seated,  Track any increasing freeplay in mechanically moving assemblies (effects of wear & tear). Further advice on trouble shooting or fault removal is contained in chapter 8 of the CyBio-FeliX User Manual. 2024...
  • Page 58 “What to do if...“ 2024...

  • Page 59: Maintenance & Care

    Maintenance & Care Maintenance & Care Safety Notes WARNING Please note that physical contact with voltage-carrying product parts may lead to body injury or even death! Turn system power off and detach power cable from the line power socket before you proceed to any kind of work for maintenance or care! Take adequate precautions to protect the product from acciden- tal restoration of power! Operating personnel are prohibited from performing work for mainte-...
  • Page 60 Maintenance & Care Dirt, e. g. dried-on liquid, may increase wear dramatically in some cas- Always maintain clean working conditions! 2024...

  • Page 61: Overview

    Maintenance & Care 9.2.1 Overview Table 8: Maintenance summary table Maintenance action Maintenance intervals Weekly Monthly required → “Cleaning housing parts” on page 55 → “Sealing Plate” on page 56 2024...

  • Page 62: Maintenance/Inspection Instructions

    “Chemical resistance” on page 22. Take particular care when using the device for processing biological samples of a risk Processing biological group because the CyBio-FeliX cannot be decontaminated as a whole. samples of a risk group In this case, we recommend applying the WHO safety recommendations (WHO Lab- oratory Biosafety Manual).

  • Page 63: Cleaning Housing Parts

    Maintenance & Care 9.3.1 Cleaning housing parts If in doubt, consult Analytik Jena GmbH+Co. KG. NOTE Pipetting heads must only be disinfected using the wipe disinfection method! For this purpose, use a lint-free cloth with a disinfecting / cleaning agent which is recommended by WHO guidelines and which is not ex- ®...

  • Page 64: Sealing Plate

    Maintenance & Care 9.3.2 Sealing Plate Where pipette tips are changed more frequently, fine fluff-ball stock or dust may settle on the sealing plate, impairing the efficiency of sealing. Procedure: 1.Do not use dusty pipette tips. 2.Check the cleanness of the sealing mat (once per week). 3.Initiate the tip replacement process to check and clean the component.

  • Page 65: Shutting Down

    Shutting Down Shutting Down CAUTION There is danger of physical injury and damage to the product if cabling is removed in power-on state! Do not remove cables as long as they are energized! Make absolutely certain that power has been turn off before you remove a cable! Make sure that all of the following requirements are met: ...
  • Page 66 Shutting Down 2024...

  • Page 67: Accessories & Spare Parts

    Accessories & Spare Parts Accessories & Spare Parts 11.1 Accessories For further information regarding accessories/spare parts, you should contact the Customer Service Department of Analytik Jena GmbH+Co. KG. 2024...
  • Page 68 Accessories & Spare Parts 2024...

  • Page 69: Waste Disposal

    Waste Disposal Waste Disposal 12.1 Consumables Consumable materials must be disposed in accordance with binding workplace safety and environmental provisions of law. 12.2 System, Components & Accessories  Unless otherwise agreed, the pipettor system, including any of its components, must be disposed in accordance with currently binding regulations of law on termination of use.
  • Page 70 Waste Disposal 2024...
  • Page 71 Cleaning powder 54 Product versions 29 Component description 29 Configuration 40 Residual Dicharge 33 Conforming Use 4 Connector panel 32 CyBio RoboTipTray 29 Safety symbol 17 Shutting Down 57 Software installation 40 Danger zones 18 Spray disinfection 55 Dangerous substances 21...
  • Page 72 2024...