ThermoFisher Scientific EVOS M3000 User Manual

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EVOS

M3000 Imaging System

™

USER GUIDE

Catalog Number AMF3000

Publication Number MAN0030066

Revision A

For Research Use Only. Not for use in diagnostic procedures.

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Summary of Contents for ThermoFisher Scientific EVOS M3000

Page 1 EVOS M3000 Imaging System ™ USER GUIDE Catalog Number AMF3000 Publication Number MAN0030066 Revision A For Research Use Only. Not for use in diagnostic procedures.
Page 2 Life Technologies Corporation | 22025 20th Ave SE Ste. 100 | Bothell, Washington 98021 USA For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. Revision history: MAN0030066 A (English) Revision Date Description 21 May 2024 New document for EVOS M3000 Imaging System.
Page 3 Contents About this guide ............7 Audience .
Page 4 Contents ■ CHAPTER 3 Capture images ........... . . 22 Overview .
Page 5 Contents ■ CHAPTER 9 Instrument care and maintenance ....... . . 42 General care .
Page 6 Contents Chemical safety ..............62 Biological hazard safety .
Page 7 About this guide CAUTION! ABBREVIATED SAFETY ALERTS. Hazard symbols and hazard types specified in procedures may be abbreviated in this document. For the complete safety information, see the “Safety” appendix in this document. IMPORTANT! Before using this product, read and understand the information in the “Safety” appendix in this document.
Page 8 About this guide Safety alert words Safety alert words Three safety alert words appear in this document at points where you need to be aware of relevant hazards. Each alert word—CAUTION, WARNING, DANGER—implies a particular level of observation or action, as defined below: CAUTION! Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury.
Page 9 Product information Product description EVOS M3000 Imaging System ™ The Invitrogen EVOS M3000 Imaging System (Cat. No. AMF3000) is a fully integrated bench-top ™ ™ imaging system for two-color fluorescence and transmitted-light applications. The EVOS M3000 Imaging System combines precision optics, a four-objective turret, a high-sensitivity ™...
Page 10 Chapter 1 Product information Standard items included Note: The light cube tool is threaded into the blank light cube (or LED light cube) and holds the light cube shipping restraint block in place. Note: EVOS LED light cubes and objectives for the EVOS M3000 Imaging System are not included ™...
Page 11 Chapter 1 Product information Instrument components and mechanical controls Instrument components and mechanical controls Front view Condenser Fixed stage LCD display Fine focus knob Objective turret Polycarbonate plastic stage insert Left view Condenser Phase ring selector LCD display Fine focus knob Objective turret EVOS M3000 Imaging System User Guide ™...
Page 12 Chapter 1 Product information Instrument components and mechanical controls Right view Condenser Phase ring selector LCD display USB 3.0 port Rear view Power switch Single-pin power input port (12 VDC, 5 A) Display port (video output) USB 2.0 port (2×) EVOS M3000 Imaging System User Guide ™...
Page 13 Chapter 1 Product information Instrument components and mechanical controls (Optional) Mechanical X-Y Stage The attachable mechanical stage is an optional feature. Go to Settings4Instrument settings4Hardware configuration4Mechanical stage for instructions on installing the Mechanical X-Y Stage (Cat. No. AMEP-5011). Vessel retention lever Polycarbonate plastic stage insert X-Y stage axis knobs EVOS...
Page 14 Chapter 1 Product information Graphical user interface (GUI) Graphical user interface (GUI) GUI layout The GUI of the system consists of the Viewing area on the left and instrument controls on the right and at the bottom. Capture and Gallery tabs, Settings button, and User and OneDrive Sign In buttons are at the top.
Page 15 Install and set up the instrument Precautions for use CAUTION! PHYSICAL INJURY HAZARD. Do not remove the instrument cover. There are no components inside the instrument that you can safely service yourself. If you suspect a problem, contact technical support. CAUTION! Before using a cleaning or decontamination method other than those recommended by Thermo Fisher Scientific, confirm with Thermo Fisher Scientific that the proposed method will not damage the instrument.
Page 16 Chapter 2 Install and set up the instrument Installation Installation Operating environment and site requirements • The dimensions of the EVOS M3000 Imaging System are 11.74 × 12.71 × 19.34 in (W × H × D) ™ (29.8 × 32.3 × 49.1 cm). The system requires a benchtop of approximately 16 x 20 in (40.04 x 50.80 cm) for best operating conditions.
Page 17 Chapter 2 Install and set up the instrument Installation Install the instrument Unpack the instrument 1. Open the shipping box and remove the accessory box. 2. Carefully lift the instrument out of the box by grasping it firmly with both hands under the instrument.
Page 18 Chapter 2 Install and set up the instrument Installation 1. If installed, using the X-axis and Y-axis stage positioning knobs, move the mechanical stage back to obtain access to the light cube shipping restraint, which is centered under the back of the stage. Note: The light cube shipping restraint is secured with the light cube tool to the blank light cube installed in the light cube turret.
Page 19 Chapter 2 Install and set up the instrument Installation Install the stage plate 1. If installed, move the X-Y stage all the way back to expose the stage opening. 2. Unpack the stage plate and insert it into the stage opening. Polycarbonate plastic stage plate Stage opening Install the UV and Condenser light shields 1.
Page 20 Chapter 2 Install and set up the instrument Installation 2. Attach the Condenser light shield to the Condenser using the groove in the Condenser barrel to clip it in place. Note: The Condenser light shield can be easily rotated 180 degrees to make the open side accessible from the front, improving access to the sample.
Page 21 Chapter 2 Install and set up the instrument Instrument set up Instrument set up Power ON the EVOS M3000 Imaging System ™ 1. Turn the instrument power switch (on the back; see “Rear view” page 12) to the ON position. 2. When the Capture tab is displayed, the EVOS M3000 Imaging System is ready to use.
Page 22 Capture images Overview Workflow Capture images Select the objective and light source (Optional) Select the camera mode (monochrome or color) Focus on the sample Find the region of interest Capture an image in a single channel Capture an image in multiple channels Overlay multiple channels (Optional) Adjust Display Settings Save...
Page 23 Chapter 3 Capture images Capture images in a single channel Capture tab The basic functions of the EVOS M3000 Imaging System, such as viewing the sample, setting optimal ™ focus, and capturing and saving images are performed in the Capture tab, which is the first screen after start-up.
Page 24 Chapter 3 Capture images Capture images in a single channel 3. Select the desired Phase option by turning the phase ring selector (“Front view” page 11). Available options include: • Oly 4x: Used for Olympus 4x phase contrast objectives (Olympus 4x PH) ™ ™...
Page 25 Chapter 3 Capture images Capture images in a single channel • Turn on the illumination by tapping a Channel button. (Light) to open the slider, then move the slider up to increase the light intensity or down to decrease the light intensity. Focus on the sample Focus is controlled during illumination using the Focus slider in the Capture tab.
Page 26 Chapter 3 Capture images Capture images in a single channel Capture another channel Repeat the steps above for illuminating, focusing, and capturing another channel. Up to 2 fluorescence channels and one transmitted light channel can be captured on the instrument. 1. Tap another channel. 2.
Page 27 Chapter 3 Capture images Capture images in a single channel (Optional) Adjust display settings The Brightness and Contrast of each channel can be adjusted to optimize the image before saving. 1. Select the channels you want to adjust by tapping the channel thumbnails as described above. 2.
Page 28 Chapter 3 Capture images Capture images in a single channel Display scale bar • Tap (Scale bar) to superimpose a scale bar over the Viewing area. – The scale bar default value is based on the magnification of the objective in use. –...
Page 29 Chapter 3 Capture images Capture images in a single channel (Optional) Select the camera mode The camera can be set to a Monochrome or Color mode. Monochrome is used for transmitted light images of live cells or any fluorescence imaging (that is, either live or fixed cells). Alternatively, Color mode is used for imaging stained tissue slices, IHC, or other colorimetric samples requiring color images taken via transmitted light.
Page 30 Save captured images Save captured images The instrument allows you to save captured images to the Gallery or an external USB drive. • To save to the Gallery: a. Tap on all of the image channels to be captured. b. Tap (Save to Gallery).
Page 31 Cell confluency Measure real-time confluency Measure the confluency of cells in real time using the Confluence tool. To enable the Confluence tool, view a sample in the Trans channel. • Tap (Confluence). When enabled, the tool will display the percentage of the field of view that contains cells.
Page 32 Chapter 5 Cell confluency Measure real-time confluency d. At the bottom of the screen, adjust the slider or tap to increase or decrease the Sensitivity. To tighten the mask and report lower confluency, move the slider to the left. To loosen the mask and report greater confluency, move the slider to the right. Note: The sensitivity adjustment can be disabled to ensure the same sensitivity across multiple sessions and users by going to Settings4Instrument settings4Screen settings4Confluency sensitivity and tapping Enabled.
Page 33 Capture videos Capture video Video capture under transmitted light can be useful if you need to capture dynamic cellular events such as cardiac myocyte beating or procedures such as splitting cell colonies for purposes of documentation or training. To enable Video capture, view the sample in Live mode (with illumination on). The (Video) tool appears to the right of the (Capture) button.
Page 34 Export files Export files Image and video files can be exported from the Capture or Gallery tabs. • To export: a. From the Capture tab, select the channels to be included in the export. Tap Export. b. From the Gallery tab, the previously saved capture file is exported without changes. Select the file, then tap Export.
Page 35 Chapter 7 Export files Select multiple files to export or delete Select multiple files to export or delete 1. To select multiple files within the Gallery tab, tap Select at the top of the screen. To select all of the images/videos in the Gallery, tap All. This will enable selection of multiple or all images with the file name of each one displaying in the side panel.
Page 36 Configure instrument settings Overview Settings Settings ( ) allows you to assign objectives to the objective turret, to calibrate objective magnification, to set image and general instrument options, add and remove light cubes, and to connect to a Wi-Fi network and to map network drives. •...
Page 37 Chapter 8 Configure instrument settings Overview Set date and time 1. From Instrument settings, click Date and time. 2. Enter the Time zone, Date format, and Time format. After each of the 3 selections, click the in the upper corner to return to the Date and time screen.
Page 38 Chapter 8 Configure instrument settings Overview 2. Click the icon at the end of the specific turret position selection (1, 2, 3, or 4). Find the objective in the Objectives list for the corresponding turret position. 3. Click Apply for the correct objective or Empty if the turret position no longer holds an objective. Calibrate white balance The setting White balance calibrates color channel lighting.
Page 39 Chapter 8 Configure instrument settings Overview Screen settings • Grid color: Choose the Capture screen image grid between white, gray, and black. The active color displays a white ring around the color circle. • Confluence Sensitivity: Enables or disables confluence sensitivity. • Sleep timer: Drag the slider bar up or down or click to increase or decrease the length of time to engage the Sleep timer.
Page 40 Chapter 8 Configure instrument settings Overview Map a network drive 1. Tap (Settings), then click Instrument settings4Network drive. 2. Enter the required Drive location (for example, //192.168.1.100/MyDocuments) with a Username and Password. 3. Tap Connect. Export error logs 1. Tap Settings4Export error logs. The Export troubleshooting screen will display with the File Name and Data destination dropdown menu.
Page 41 Chapter 8 Configure instrument settings Overview 4. Start the update by tapping Yes. Note: During the update process the instrument may restart multiple times. The update process is complete when the Capture tab is displayed. EVOS M3000 Imaging System User Guide ™...
Page 42 Instrument care and maintenance General care • When cleaning optical elements, use only optical-grade materials to avoid scratching soft lens coatings. • Clean the stage as needed with paper towels or Kimwipes tissues dampened with 70% ethanol. ™ • Use the appropriate cleaning solutions for each component, as indicated in the Decontamination Procedures below.
Page 43 Chapter 9 Instrument care and maintenance Objective lens care Objective lens care Clean each objective periodically or when necessary with an optical-grade swab and a pre-moistened lens wipe (or lens paper moistened with lens cleaning solution). To avoid scratching the soft lens coatings, use only optical-grade cleaning materials and do not rub the lens.
Page 44 Chapter 9 Instrument care and maintenance Change the objectives Change LED light cube 1. Go to Settings4Instrument settings4Light cubes. 2. Select the position of the Light cube you want to change. 3. Move the stage (if mechanical stage is installed) back to allow access to the light cube, which is centered under the back of the stage.
Page 45 Chapter 9 Instrument care and maintenance Change the objectives 3. Screw the new objective into the available position in the objective turret. Note the part number of the objective and the turret position. 4. Go to Instrument settings4Hardware configuration4Objectives, select the active turrent position with the new objective, then find the appropriate objective in the Objectives list.
Page 46 Troubleshooting Note: For additional technical support, contact your local EVOS distributor. If you do not have your ™ distributor information, visit thermofisher.com/evos or contact Technical Support. Image quality issues Observation Recommended action Transmitted light image is too dim (at higher Remove the condenser slider, if one is in place.
Page 47 Appendix A Troubleshooting Software interface issues Software interface issues Note: We recommend keeping the EVOS M3000 Imaging System up to date with the latest software. ™ Observations Recommended actions Some of the software controls The controls available on the EVOS M3000 Imaging System are ™...
Page 48 Graphical user interface (GUI) Capture screen Figure 2 EVOS M3000 Imaging System Capture screen at start up (with no channel selected) ™ Image pane Adjust image display Sign In/User Confluence tool Wireless connection status Save to Capture tab Export Gallery tab Toggle pseudocolor Settings Display scale bar Objective and phase indicator...
Page 49 Appendix B Graphical user interface (GUI) Capture screen Figure 3 EVOS M3000 Imaging System Capture screen with Live camera feed from fluorescence light ™ channel (GFP channel selected) Image page (showing live camera display from the Capture movie control selected channel) Capture control Live camera display indicator Toggle pseudocolor Transmitted light channel Display scale bar...
Page 50 Appendix B Graphical user interface (GUI) Gallery screen Gallery screen Figure 4 EVOS M3000 Imaging System Gallery screen (with captured images) ™ Gallery pane Channel(s) Selected image Confluence Video image (displays time length) Objective Select Phase Delete View Image name Export Date/time EVOS M3000 Imaging System User Guide ™...
Page 51 Specifications Instrument specifications Technical specifications Operating conditions Indoor use only Operating temperature 4°–32°C (40°–90°F) Operating humidity <90%, non-condensing Operating power 100–240 VAC, 1.5 A Frequency 50–60 Hz Electrical input 12 VDC, 5 A Optics Infinity-corrected optical system; RMS-threaded objectives with 45 mm parfocal distance Illumination Adjustable intensity LED (>50,000-hour life per light cube) 2-position chamber for up to 2 fluorescent LED cubes...
Page 52 Appendix C Specifications Physical dimensions (continued) Technical specifications Networking capability Connection through Microsoft Windows Server Message Block (SMB) network ™ ™ wirelessly using the supplied Wi-Fi adaptor Cloud connectivity Use the Thermo Fisher Connect Platform or Microsoft OneDrive database for ™ ™ ™...
Page 53 Safety WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document.
Page 54 Appendix D Safety Symbols on this instrument Standard safety symbols Symbol and description CAUTION! Risk of danger. Consult the manual for further safety information. CAUTION! Risk of electrical shock. CAUTION! Potential biohazard. CAUTION! Ultraviolet light. Symbole et description MISE EN GARDE ! Risque de danger. Consulter le manuel pour d’autres renseignements de sécurité.
Page 55 Appendix D Safety Symbols on this instrument Location of labels Label and location Figure 5 Instrument information label (back panel) Control and connection symbols Symbols and descriptions On (Power) Off (Power) Protective conductor terminal (main ground) Alternating current EVOS M3000 Imaging System User Guide ™...
Page 56 Appendix D Safety Safety information for instruments not manufactured by Thermo Fisher Scientific Conformity symbols Conformity mark Description Indicates conformity with safety requirements for Canada and U.S.A. Indicates conformity with China RoHS requirements. Indicates conformity with European Union requirements. Indicates conformity with Australian standards for electromagnetic compatibility. Indicates conformity with the WEEE Directive 2012/19/EU.
Page 57 Appendix D Safety Instrument safety Physical injury CAUTION! Moving and Lifting Injury. Improper lifting can cause painful and permanent back injury. Things to consider before lifting or moving the instrument or accessories: · Depending on the weight, moving or lifting may require two or more persons. ·...
Page 58 Appendix D Safety Instrument safety Electrical safety WARNING! Ensure appropriate electrical supply. For safe operation of the instrument: · Plug the system into a properly grounded receptacle with adequate current capacity. · Ensure the electrical supply is of suitable voltage. · Never operate the instrument with the ground disconnected. Grounding continuity is required for safe operation of the instrument.
Page 59 Appendix D Safety Safety and electromagnetic compatibility (EMC) standards Cleaning and decontamination CAUTION! Cleaning and Decontamination. Use only the cleaning and decontamination methods that are specified in the manufacturer user documentation. It is the responsibility of the operator (or other responsible person) to ensure that the following requirements are met: ·...
Page 60 Appendix D Safety Safety and electromagnetic compatibility (EMC) standards Safety standards Reference Description EU Directive 2014/35/EU European Union “Low Voltage Directive” EN 61010-1 Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 1: General requirements UL 61010-1 CAN/CSA C22.2 No. 61010-1 EN 61010-2-081 Safety requirements for electrical equipment for measurement, control and laboratory use –...
Page 61 Appendix D Safety Safety and electromagnetic compatibility (EMC) standards (continued) Reference Description ICES-001, Issue 4 Industrial, Scientific and Medical (ISM) Radio Frequency Generators FCC Part 15 Subpart B U.S. Standard Radio Frequency Devices (47 CFR) This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.
Page 62 Appendix D Safety Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: · Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials.
Page 63 Appendix D Safety Biological hazard safety · Veiller à utiliser des récipients à déchets primaire et secondaire. (Le récipient primaire contient les déchets immédiats, le récipient secondaire contient les fuites et les écoulements du récipient pri maire. Les deux récipients doivent être compatibles avec les matériaux mis au rebut et conformes aux exigences locales, nationales et communautaires en matière de confinement des récipients.) ·...
Page 64 Documentation and support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •...
Page 65 EVOS M3000 Imaging System_UG_MAN0030066-v2-GUID-CDC7C217-D87B-4571- AA93-17C03CC6FE0A-2024/05/21 22:22:10 en 22:55:23.413+01:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 21 May 2024...

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