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Version:6.0
DR-200Bc
Microplate Reader
Instruction Manual
English
Wuxi Hiwell-Diatek Instruments Co.,Ltd.

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Summary of Contents for Diatek DR-200Bc

  • Page 1 Version:6.0 DR-200Bc Microplate Reader Instruction Manual English Wuxi Hiwell-Diatek Instruments Co.,Ltd.
  • Page 2: Table Of Contents

    Table of Contents SECTION 1 IMPORTANT SAFETY INSTRUCTION .............. 1 1.2 S ............................2 YMBOL SECTION 2 INTRODUCTION OF INSTRUMENT ..............2 2.1 A ........................2 PPLICATION COPE 2.2 M ........................3 ERFORMANCE 2.3 M .......................... 3 UNCTION 2.4 M ..................
  • Page 3 8.1 T ..........................17 EST SETTING 8.2 P ..........................18 LATE 8.3 T ............................ 18 EMPLET 8.4 W .......................... 18 OSITION 8.5 P ..........................20 LATE TEST 8.6 R ......................20 ESULT VIEW AND PRINT SECTION 9 SAMPLE INFORMATION ................21 9.1 B ........................
  • Page 4: Important Safety Instruction

    Section 1 Important Safety Instruction Dangerous or wrong using method may cause electric injuries, burns, fire or other disaster. Basic safety protection including all the followings should be always obeyed. When the instruments are used near children, disabled people or samples, rigorous monitoring should be performed.
  • Page 5: Symbol

    The symbol is marked on the machine nameplate to Service life indicate the period of use of the machine. Section 2 Introduction of instrument 2.1 Application Scope DR-200Bc Microplate Reader is applied in clinical optical method for enzyme-linked immunosorbent assay.
  • Page 6: Main Performance

    2.2 Main Performance Wavelength accuracy and transmission character of the filters should meet the following requirements: Item Indicator ±1.0 Indication error of wavelength(nm) ≤8 Half width(nm) ≥35 Peak transmittance(%) -Measured value stability:±0.003A -Absorbance indication error (Accuracy):±0.008A -Absorbance repeatability: ≤0.2% -Sensibility:≥0.01(L/mg) -Channel error: ≤0.01A 2.3 Main Function -Large screen interface, touch screen and touch pen input mode make it convenient for users to input...
  • Page 7: Specification And Technical Parameter

    ① Power indicator light : After the instrument is power on, the indicator light is lit. ② Touch screen: Show software interface. Users can press the screen with fingers for variable operation ③ Internal thermal printer: Plastic cover plate. Paper delivery when using internal thermal printer. You can open the cover plate to replace paper.
  • Page 8: Instrument Installation

    10 minutes Warm-up time: Computer system: ARM core series Programming More than 500 items: Communication RS-232 serial、Parallel printer、USB、Mouse and keyboard interface interface: LCD dispaly screen Display: Touch Screen ,Touch pen Input mode: Memory 200,000 test data Capability: Section 3 Instrument Installation 3.1 Instrument Packaging and unpacking Unpack the instrument package, and take out the instrument.
  • Page 9: Start Installing

    protective grounding of the machine unified using with logo .The AC power supply must be stable, don’t share power with high-power electrical appliances. When unplug the power line,, must grasp the plug itself, rather than the power line. If discover there is smoke, strange smell or strange sound, immediately shut off the power and contact the seller.
  • Page 10: Notice And Direction Of Sample Collection And Handling

    Input Tool and Operation 4.1 Touch Screen&pen DR-200Bc Microplate Reader uses touch screen & touch pen as basic input device. The user operates within the scope of the screen. Basic operations only have 1 kinds: (1) Click: Touch the screen with a finger or pen then leave;...
  • Page 11: Digital Soft Keyboard

    Warning: DR-200Bc Microplate Reader use touch screen: do not use sharp or hardness of objects (such as metal, glass, nails, etc.) touch screen surface; Part of touch screen is glass, do not exert great impact on touch screen, so as to avoid damage.
  • Page 12: Date、Time Soft Keyboard

    4.4 Date、time soft keyboard For entering date and time, sliding the number up or down can change the current selection state. 4.5 Formula soft keyboard Used to enter a qualitative formula. Section 5 Start-up 5.1 Procedure of start-up Turn on the power switch at the rear panel. Wait about 6 seconds, the instrument will perform an initialization process.
  • Page 13: System

    Section 6 System Click “System” button in the main menu and go into the system setting menu. 6.1 Main Menu Select the “System” button and go into the system setting menu: 1)Serial No.(ID): This number is read-only and cannot be modified; 2)Company Name: No more than 20 characters (including Chinese and English characters);...
  • Page 14: Data Delete

    Click the ‘Maintenance’ button to go into the system maintenance menu.  Add Filters: First select the number of filters, then fill each filter wavelength corresponding each well, click "OK" button.  Lamp Test: The function is mainly used to replace the lamp. After selected the filter, click the "Start"...
  • Page 15: Item Setup

    Item Setup In the main menu, press the “Item Setup” button to enter the item list window: DR-200Bc Microplate Reader all items are defined by the user. Items that have been set by the user are displayed in the window list.
  • Page 16: Analysis Method

    calculated. Note: the kinetic method uses only one wavelength. 7.2 Analysis method There are 9 analysis methods, divided into the following 3 categories: 7.2.1 O.D. mode The absorbance of the sample is measured and output directly. 7.2.2 Qualitative(Cut-Off) mode  ...
  • Page 17: Add New Item

    Y  Exp. Regress: allow set 2-8 standard samples, an exponential curve is regressed by these standard points as a calibration curve,all the standard sample O.D. values must be >0 .  Y  If the absorbance value is logarithmic ( ), the exponential regression can be transformed ...
  • Page 18 ● Sort No.: Define the sort of the item in the patient report. The small No. is in the front of the list. The default value is 100. ● ABS analysis methods:Press the “OK ”to save the item information and press the “Cancel” to exit the settings for the current items;...
  • Page 19 In addition to the formulas Cut-Off and ABS, click the “Page 2” to display the quantitative settings interface, and you can modify the following parameters: ● STD numbers: In addition to Single regress only can set 1 standard samples ,others can set 2~8 standard samples.
  • Page 20: Modify The Item

    When adding a new item, the new item will not be created if the items number has exceeded 500, the system prompts that “The number of items is full”. The operation of adding a new item is the same as the procedure for changing an old project.
  • Page 21: Plate Well

    ● Plate Direction:select Horizontal or Vertical position; ● Moving Mode:select the plate continuous moving or stepping moving; ● Shaking speed:select the slow、medium or fast shaking speed; ● Shaking time:select the time of shaking plate, user-defined area is from 10 to 180 second. ●...
  • Page 22 The DR-200Bc Microplate Reader allows users to set wells at any position within the 96 orifice plate. -The procedure of the position: Click “Set” to select the item; Click “S,B,N,P,D,C” to select the type of the sample; If you need multi item board layout., click on the "2nd" or ">>" button, select the item and layout again.
  • Page 23: Plate Test

    set the starting position, the end position and the initial sample number, and press "OK". -Templet Press "Set" ,then press “Board Templet ” to enter the template setting window. The template is a specific type of cloth board, which can be set according to the usage habit, simplifying the daily fabric operation.
  • Page 24: Sample Information

    Click "Layout" to check the condition of layout board. Click "ABS" to view the absorbance value and click the single sample well position to modify the sample absorbance value. Note: If the measured absorbance is > 4.000 A, it is shown as 4.00 *; < 0.000A will show as 0.00 *. Click “Qualitative”...
  • Page 25: Search

    Name: 6 bytes in length; Sex: Male and female, default is blank; Age: 0 - 150 years old, default 0 years old; Bed No.: 9 bytes in length; Medical record No: 9 bytes in length; Sample type: Set in the dictionary function module, here only needs to select one item; Submit department: Set in the dictionary function module, here only needs to select one item;...
  • Page 26: Item Report

    ● After select a patient item,the first column of the table will appear the words "P", click again can cancel it .Can click “ Select all ” , add "P" to multiple patient items, click “Print” can continuously print multiple patient report. Click “Last page ”...
  • Page 27: Board Report

    10.3 Board Report Press the "Board report" button and click "ok" to enter the whole board test result search. Search according to Plate No. and Test date . The contents of the board report are in accordance with the test results. 10.4 Standard Curve Press the “Standard curve”...
  • Page 28: Report

    The quantitative items show the concentration and absorbance of each standard sample and show curves simultaneously. Section 11 Report In the main interface, press the "Report" button to enter the comprehensive report window. This button is ashortcut to check patient reports for the day. Section 12 PC Control 12.1 Software installation...
  • Page 29: Software Running Environment

    mode, the “Test” button on the main interface will be clicked invalid. 12.2 Software running environment Operating system: Windows XP,7,8,10 Hardware environment: Pentium III350 above, memory 128M, hard disk excess capacity of 500M or more, resolution 800*600 and above Mode of communication: Serial communication,USB Communication interface: RS232 serial port,USB Baud rate: 19200 12.3 Software serial communication protocol and code...
  • Page 30 0x010B Light on & off time 0x010C Read the serial number (ID) 0x010D Serial number 0x0118 Long time vibrating plate 3)Instruction sending and answering Command Data Response Data Timeout time Other instructions Reset 0x0010 15 seconds Read front end 0x0003 bytes version 1 seconds...
  • Page 31: Shut Down

    14.1 Summary DR-200Bc microplate reader is a precision clinical analytical instrument. To make the instrument keep a good status, user must do routine maintenance well. Although maintenance of DR-200Bc microplate reader is easy, user needs do it carefully. 14.2 Cleaning instrument ●...
  • Page 32: Simple Fault Handling

    6) Return upper cover and restart the instrument. : Note The lamp must be purchased with the specified brand and model. 14.4 Simple fault handling Phenomenon or hints Reason analysis Method   Bulb not lighting Bulb power supply Check whether supply abnormal voltage is 8V ...
  • Page 33 rotating  data board, Holzer components can not connect the magnetic steel of the plate holder   External printer unable Printer power is not normal Check if power plug is loose  to start Check ON/OFF button  Check if printer type is setting correct External printer unable ...
  • Page 34  The quantity of standard wells does not match with  Carefully verify the number the quantity of standard of standard items in the Standard layout error which is setted in the reagent user manual project in the number of items set in the standard quantity ...

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