Sol COUNT1 User Manual

Automated cell counter

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Automated Cell Counter
[Model Name : SOL COUNT1]
Semiconductors for Lens-Free Sensors
User Manual(Ver.1.0)
ⓒ 2021 Sol Inc. All rights reserved.
1

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Summary of Contents for Sol COUNT1

  • Page 1 Automated Cell Counter [Model Name : SOL COUNT1] Semiconductors for Lens-Free Sensors User Manual(Ver.1.0) ⓒ 2021 Sol Inc. All rights reserved.
  • Page 2: Table Of Contents

    2. Product information --------------------------------------------------------------------------- 4 2.1. Product overview -------------------------------------------------------------------------- 4 2.2. Product configuration --------------------------------------------------------------------- 4 2.3. SOL COUNT Automated Cell Counter features ------------------------------------------ 5 2.4. Upon receiving the product package ---------------------------------------------------- 5 2.5. Device exterior components -------------------------------------------------------------- 6 2.6. Environmental requirements -------------------------------------------------------------- 7 2.7.
  • Page 3 7.2. Software update ------------------------------------------------------------------------- 17 7.3. Wi-Fi -------------------------------------------------------------------------------------- 17 8. Maintenance ---------------------------------------------------------------------------------- 18 8.1. Device care ------------------------------------------------------------------------------- 18 8.2. Cleaning the SOL COUNT Automated Cell Counter ----------------------------------- 19 8.3. Battery ------------------------------------------------------------------------------------ 21 9. Troubleshooting ------------------------------------------------------------------------------ 22 10. Safety standards ------------------------------------------------------------------------------ 24 10.1.
  • Page 4: Before Use

    1.2. Battery heating while in use or charging 1) If there is heat while charging the battery - The SOL COUNT Automated Cell Counter and charger might heat up during charging. This is normal, and the life and function of the product are unaffected.
  • Page 5: Safety Symbols & Abbreviations

    The disposable four-chamber cartridge makes it easy to use and more cost-effective than any competitor. In addition, SOL COUNT Automated Cell Counter is a compact and portable device. 2.2. Product Features...
  • Page 6: Product Configuration

    Damage claims must be filed with the package carrier. In-transit damage is not covered by the warranty. Store the device and cartridge at a normal temperature. (15-25 2.4. Product Configuration Picture Component Quantity SOL COUNT Automated Cell Counter 1 each SOL COUNT Disposable Cartridge (50ea/box) 1 box...
  • Page 7: Device Exterior Components

    ② ① Touchscreen display: The 7-inch capacitive touchscreen display displays cell count data, has function buttons, and is the main user interface of the SOL COUNT Automated Cell Counter. ② Cartridge tray: The SOL COUNT cartridge containing the sample is inserted into the device using this tray.
  • Page 8: Getting Started

    to an external device for record keeping and printing. You can use the USB drive supplied with the device or any other standard, FAT 32-formatted USB drive for data transfer. If needed, you can plug in a USB mouse into the rear USB port for device control. ⑥...
  • Page 9 “ menu icon in the upper right of the PREVIEW screen to display the pop-up menu, pictured below. - Login: Log in or out with the ID/PW made on the SOL website (http://www.sol.re.kr) or the SOL COUNT Automated Cell Counter.
  • Page 10 - Info: You can update the serial number, S/W version, and S/W related to the product. 3.4. Connecting to the Internet ※ In order to keep the SOL COUNT Automated Cell Counter up to date and ensure optimal performance, an ethernet or Wi-Fi connection is required.
  • Page 11 Cell Counter, and when the “Sign up” button is selected, you can create an ID/PW by registering as a new member. - When you log in, you can save the result image of the SOL COUNT Automated Cell Counter in the SOL developer platform by linking with the SOL developer platform...
  • Page 12: Cell Viability Test And Cell Counting

    3.7. Checking Product Information When you select the Product menu in the pop-up menu, you can see a list of products related to the SOL COUNT Automated Cell Counter. 4. Cell Viability Test and Cell Counting 4.1. Preparation 1) For measurement of total cell concentration and viability: The measurement range is 1 x –...
  • Page 13: Loading The Cartridge

    ※ CAUTION: A: Chambers 1 and 3 B: Chambers 2 and 4 ※ When the sample is loaded into the SOL cartridge chamber. It is ready to be put into the SOL COUNT cartridge tray. ※ CAUTION: Each chamber in the SOL COUNT Cartridge has a 10 ul sample capacity. If the sample exceeds this amount, the chamber may overflow.
  • Page 14: The Count Procedure

    4.4. The Count Procedure 1) LOAD the SOL COUNT cartridge with your trypan blue stained sample, open the cartridge tray of the device and insert the cartridge into the cartridge tray. Push the cartridge tray until it closes.
  • Page 15 (위의 이미지 변경) 5) Press the “Capture” button. If you want to view files that have been saved to the USB, press the “Open Files” button. 6) To get the cell count result, set the type (cultured cell or beads) and dilution factor. If the sample is without trypan blue staining, set it to 1x.
  • Page 16: Next Steps

    5.1. View results screen 1) The data analysis results (total cell concentration, live and dead cell concentration, viability %) are displayed on the SOL COUNT screen. 2) When performing a cell count and viability check with a trypan blue-stained sample, the total cell concentration is calculated by multiplying by the dilution factor of 2x.
  • Page 17: Viewing The Graph

    6. Saving results 6.1. Saving count results 1) SOL COUNT Automated Cell Counter allows you to save your data and images using a USB flash drive. 2) To save your data, insert the SOL COUNT Automated Cell Counter USB drive (or...
  • Page 18 equivalent) into an available USB port on the device. There are two USB ports located on the back of the device. The first USB drive connected will be set as the preferred saving location. 3) Press the “Save Files” button on the cell counting RESULT screen as shown below to move to the save screen.
  • Page 19: Report

    2) The top section of the report contains an origin image along with the viability percentage and the number of total, live, and dead cells. 3) At the bottom of the report, a result image contains the circle from the cell counter algorithm developed by SOL Inc. Software Update...
  • Page 20 1) When a new software version is released, the SOL COUNT Automated Cell Counter displays the software update pop-up as shown below after booting is complete. 2) Select the Info menu in the pop-up menu and press the “S/W Update” button to update.
  • Page 21 8. Product Specifications 1) Product Dimensions (mm) 180 (W) x 120 (L) x 78 (H) Weight 1.8 kg LCD Standard 7” 1024 x 600 HD IPS Input 5V dc 2A / 3.7V 4400 mAH Image Sensor 1/4” 8M Pixel x 2 LED Wavelength 465-485 nm Battery...
  • Page 22 4) In case of breakdown or damage due to external impact or dropping. 5) In the event of a breakdown due to repair by partnerignated by SOL Inc. 6) In case of failure due to the use of consumables or repair materials not specified by SOL Inc.
  • Page 23: Wi-Fi

    ※ First of all, ensure that you have downloaded the latest version of the software via Wi-Fi. You can download the latest version of the software from the SOL COUNT Automated Cell Counter app. Please establish a Wi-Fi connection before downloading.
  • Page 24 • If you are pipetting different samples from the same cell sample, variability in cell counts could be due to pipetting or mixing. • Pipet the cell sample and trypan blue mixture up and down several times to make sure it is Variable counts of the same well mixed.
  • Page 25 • Do not over or under fill the cartridge chambers. Carefully load Improper sample loading the chambers with 10 uL of cell suspension • Use a new SOL COUNT Damaged or contaminated cartridge. Wear gloves and cartridge handle by the edges to avoid smudging and contamination.
  • Page 26 • If there are many samples to be measured, it is recommended to connect the power cable before measuring. • Check whether the USB is Errors in the storage setup plugged in, the folder to be process saved is set, and the image to be saved is properly selected.
  • Page 27: Safety Standards

    Users must be aware of and follow the conditions described in this manual for operating the device. The protection provided by the device may be impaired if the device is used in a manner not specified by Sol Inc.
  • Page 28: Korean Standards

    13.2. Korean Standards Description The KC certification mark indicates that this device conforms with Korea’s product safety requirements for electrical and electronic devices and components for which this marking is required. 13.3. US(FCC) Standards FCC ID : 2A9DGSOLCOUNT1 FCC NOTICE TO USERS: This device complies with Part 15 of the FCC Rules.
  • Page 29 14. Technical Support Information For product information and technical support, including manuals and FAQs, please contact us by telephone or email. SOL Inc Address: BK Tower 2F, 28, Beobwon-ro 11-gil, Songpa-gu, Seoul 05836, Republic of Korea Tel: +82-2-6949-2960 Fax: +82-2-6949-2965 Email: info@sol.re.kr...
  • Page 30: Ordering Information

    Product Name Composition/Unit SOL COUNT Automated Cell Counter device 1 ea., SOL COU 1 SOL COUNT SOL Cartridge 1 box, C- type cable, USB drive SOL COU 2 SOL Cartridge 1 box 50 ea./1 box SOL COU 3 SOL Cartridge 2 box 50 ea./1 box...

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