Rise V-FC User Manual

Video-based flow cytometer

Advertisement

Quick Links

Approved/Godkänd
Dag Ilver
Issued by/Utfärdare
Christian Jonasson, Dag Ilver
Distribution
Video-based Flow Cytometer
Contents
1
Introduction ...........................................................................................................................................2
2
Sensor system ........................................................................................................................................2
3
Instrument ..............................................................................................................................................2
3.1
Dimensions ....................................................................................................................................2
3.2
Front panel .....................................................................................................................................3
3.3
Built-in hardware ...........................................................................................................................4
3.3.1
Optical detection module .......................................................................................................4
3.4
Flow diagram .................................................................................................................................5
3.5
Computer .......................................................................................................................................5
3.5.1
Software User Interface .........................................................................................................6
3.5.2
Buttons...................................................................................................................................6
3.5.3
Graphs ...................................................................................................................................7
3.5.4
Program Menu .......................................................................................................................7
3.5.5
Input parameters ....................................................................................................................7
3.5.6
Settings file ............................................................................................................................9
4
Starting up the instrument ...................................................................................................................10
5
Turning of the instrument ....................................................................................................................10
6
Measurement sequence ........................................................................................................................11
6.1
Start a measurement from the front panel ...................................................................................11
6.2
Start a manual measurement from GUI .......................................................................................11
6.3
Log files .......................................................................................................................................11
6.4
Analog signal out.........................................................................................................................12
7
Remote connection via Modbus ..........................................................................................................13
8
How to Change the Instrument IP Address .........................................................................................14
8.1
To enable internet access (if network allows) .............................................................................16
Date/Datum
2022-08-31
Type of document/Dokumenttyp
User Guide
Reference/Referens
V-FC UserGuide R1
For information only/För kännedom
User Guide
Rev
Page/Sida
R1
1(16)

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the V-FC and is the answer not in the manual?

Questions and answers

Summary of Contents for Rise V-FC

  • Page 1: Table Of Contents

    2022-08-31 1(16) Approved/Godkänd Type of document/Dokumenttyp Dag Ilver User Guide Issued by/Utfärdare Reference/Referens Christian Jonasson, Dag Ilver V-FC UserGuide R1 Distribution For information only/För kännedom Video-based Flow Cytometer User Guide Contents Introduction ............................2 Sensor system ............................2 Instrument ..............................2 Dimensions ............................2 Front panel .............................3...
  • Page 2: Introduction

    Page/Sida 1 Introduction This document describes how to use the second release of the Video-based Flow Cytometer (V-FC) de- veloped by RISE AB. The instrument is designed to detect contamination of drinking water by sewage water containing E.coil, chosen as faecal indicator bacteria. In other applications the instrument is used for detection of other bacteria or auto-fluorescent microalgae.
  • Page 3: Front Panel

    Page/Sida 3.2 Front panel The instrument front panel, showed in Figure 2, has one ON/OFF-switch, two buttons and seven LEDs. All the components are described in table below. The front door can be open using a key. Figure 2. Instrument front panel. Table 1.
  • Page 4: Built-In Hardware

    Page/Sida LED on = “Auto mode” Auto Indicates the current state LED off = “Manual mode” of the system is. Failure System error indicator 3.3 Built-in hardware Figure 3 shows an overview of the hardware inside the instrument, including AC/DC converters, embed- ded computer (PC), I/O signal device, mixing stage, incubator (holding a ~39°...
  • Page 5: Flow Diagram

    Page/Sida Figure 4. The optical detection module inside the instrument consists of a laser source (532 nm) at the excitation side and a CCD camera with emission filter on the detection side. 3.4 Flow diagram The flow diagram in Figure 5 shows how the water sample is pumped into the system and mixed with the reagents before it flows further into the incubator.
  • Page 6: Software User Interface

    Page/Sida 3.5.1 Software User Interface The built-in software has a graphical user interface (GUI), which enables the user to control the meas- urement and visualize the results and show the progress of the video processing. This requires a screen and mouse/keyboard are connected. From the GUI the user can manually start/stop the pumps, control the video capture and start the pre- programmed measurement sequence.
  • Page 7: Graphs

    Page/Sida Pump #2 Start/stop Pump 2 (Reagent) Pump #3 Start/stop Pump 3 (Mixing) Pump #4 Not in use Re-open port Opens specified COM-port for pump control (Only to be used if computer fails to connect to the pump control board during program start-up) 3.5.3 Graphs...
  • Page 8 Page/Sida Pump 3 (measurement) duration (min) during meas- Pump #3 hold time urement sequence Incubation time Time of the incubation step (min) COM-port Pump COM-port...
  • Page 9: Settings File

    Page/Sida 3.5.6 Settings file Default settings (Figure 7) are loaded from file (settings.ini) during program start-up. The file is located in the program directory and contains default settings for example; COM-port number, pump volumes, flow rates, incubation time, camera settings and alarm levels. Figure 7.
  • Page 10: Starting Up The Instrument

    Page/Sida 4 Starting up the instrument 1. Make sure the instrument is connected to the mains (220 V). 2. Make sure the button “Embedded Computer” is set to “1” (ON) 3. Use the power switch in the front panel to turn on the system power → The “Power” LED will now shine 4.
  • Page 11: Measurement Sequence

    Page/Sida 6 Measurement sequence The pre-programmed measurement sequence will automatically pump the water sample into the mixing container and add the reagent. After the incubation the mixture will be pumped into the detection module and the video analysis will be started. When the measurement is finished data will be stored into the “da- ta”...
  • Page 12: Analog Signal Out

    Page/Sida Figure 8. Thresholded linogram showing a summary of all processed video data for the last measurement. Each island is counted as one particle/bacteria. Figure 9. Plot showing the number of counts versus the threshold value. 6.4 Analog signal out The instrument has an analog current output, 4-20 mA.
  • Page 13: Remote Connection Via Modbus

    Page/Sida 7 Remote connection via Modbus The IP address for the Flow Cytometer (FC#3) was assigned to 192.168.221.111 in order to be accessible locally over an Ethernet cable. The user should be able to connect to the instrument whose Station-ID is chosen to be 46 using the IPv4 protocol.
  • Page 14: How To Change The Instrument Ip Address

    Page/Sida 8 How to Change the Instrument IP Address 1. Make sure the ethernet cable is connected to the 2nd LAN port on the embedded PC 2. On the embedded PC: Open "Network and Sharing Center" from Control Panel 3. Click on the "External PC interface 4.
  • Page 15 Page/Sida 6. Choose "Use the following IP address" and enter the IP address and netmask:...
  • Page 16: To Enable Internet Access (If Network Allows)

    Page/Sida 8.1 To enable internet access (if network allows) 1. Do all above, 1-5 2. Choose: "Obtain an IP address automatically" 3. Click "OK"...

This manual is also suitable for:

1057.500

Table of Contents