Advertisement
Quick Links
Advertisement
Subscribe to Our Youtube Channel
Related Manuals for Scion Instruments LC6000
Summary of Contents for Scion Instruments LC6000
- Page 1 LC6000 Troubleshooting Guide...
- Page 2 20 column volumes Retained peaks look like baseline drift Use a guard column • • Thoroughly flush the column with strong • solvent before next injection Company Confidential. ©SCION Instruments 2021...
- Page 3 Prolonged retention of target compound(s) Modify mobile phase composition • • Use appropriate mobile phase buffer • Use a different stationary phase column • Blocked column Reverse phase flush column with strong • • solvent Replace column • Company Confidential. ©SCION Instruments 2021...
- Page 4 Dilute sample (too concentrated, sample • overload) Carry Over Increase run time • • Increase gradient • Flush system with strong solvent • Ghost peaks Prepare fresh mobile phase • • Reduce injection volume • Company Confidential. ©SCION Instruments 2021...
- Page 5 Sample loop blocked Flush with strong solvent • • Replace sample loop • Waste tubing blocked Flush with strong solvent • • Replace waste tubing • Syringe failure Tighten syringe gently • • Replace syringe • Company Confidential. ©SCION Instruments 2021...
- Page 6 Prime pump with mobile phase • No mobile phase Make up new mobile phase • • Check vales not working Replace check valves • • Leak Find source of the leak and tighten or replace • • fittings Company Confidential. ©SCION Instruments 2021...
- Page 7 Check valves are faulty Replace check valves • • Pump seal failure Replace seal • • Leak Identify leak and tighten/replace fittings • • Mobile phase changes Fully purge system with new mobile phase • • Company Confidential. ©SCION Instruments 2021...
- Page 8 Use a narrower ID tubing • Guard column / column contaminated Replace guard column / column • • No resolution of two peaks Change column to improve separation • • Column temperature too low Increase column temperature • • Company Confidential. ©SCION Instruments 2021...
- Page 9 • • Contaminated guard column / column Replace guard column / column • • Incorrect mobile phase composition Prepare new mobile phase • • Air in system Purge system • • Degas mobile phase • Company Confidential. ©SCION Instruments 2021...
- Page 10 Livingston Business Centre Kirkton South Road, Livingston West Lothian EH54 7FA Scotland, UK Phone +44 (0)1506 300 200 sales-eu@scioninstruments.com The Netherlands Amundsenweg 22-24 4462 GP Goes, The Netherlands Phone +31 (0)113 287 600 sales-eu@scioninstruments.com ©SCION Instruments 2021 Company Confidential. ©SCION Instruments 2021...
Need help?
Do you have a question about the LC6000 and is the answer not in the manual?
Questions and answers