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Summary of Contents for Apostle Magtouch 3000
- Page 1 Magtouch 3000 Nucleic Acid Purification System Operation Manual V 3.0 Apr 2024...
- Page 2 Version Record Version No. Date Description V1.0 2023-08-29 Initial Release Updated the software interface and V2.0 2023-12-12 parameters V2.1 2024-03-03 Formatting V3.0 Formatting...
- Page 3 C O N T E N T S 01 Introduction and Safety 05 Summary 10 Installation and site conditions 12 Extraction process outline 16 Running a program 17 Writing protocols 20 Sanitize 21 System and Settings 22 Maintenance...
- Page 4 Keep it for future reference. Reading this manual alone does not qualify individuals to use this instrument. Opening Check Please check the instrument and accessories against the packing list when you open the packing case. If anything is missing or damaged, please contact Apostle. Contact Us Telephone: 1-888-305-3218 Website: http://apostlebio.com...
- Page 5 丨Introduction and Safety Instrument Description The Magtouch 3000 is our newest fully automated nucleic acid extractor for DNA/RNA extraction from FFPE, cfDNA and other samples. Its main functions are mixing, heating, and magnetic absorption. Magnetic beads are transferred by adsorption on magnet rods, or inside of the mixing .
- Page 6 The user is prohibited from disassembling the instrument, except for maintenance covered in the user manual. Unauthorized disassembly will void the warranty and may cause electric shock. Apostle will handle all repairs. Electrical Safety Before installing the instrument, make sure that the voltage and rated current load of the power supply are consistent with the requirements of the instrument.
- Page 7 Instrument is functioning abnormally, including unusual sounds or smells, or ● If there are any spills or water damage. After disconnecting the instrument, contact Apostle to arrange repairs. Ultraviolet Hazard There is a germicidal UV lamp in the instrument for disinfection.
- Page 8 丨Summary 1丨Specifications Model Magtouch 3000 Throughput 1-24 samples Sample well volume 2-15 mL Minimum elution volume 20 µL Stability CV ≤ 5% Maximum heating temperature 120°C Temperature uniformity 3°C Temperature stability 1°C Temperature accuracy 1°C Pipette precision, 20-100 µL Pipette precision, 100-500 µL 1.5%...
- Page 9 丨Summary 2丨Dimensions 2.1 Dimensions 2.2 External Features 2.3 External Features, Rear 丨06 Apostle, Inc...
- Page 10 Pipette magnets: Small neodymium magnets used with the pipette tip to collect beads. Magnet rods and sleeve holders: Strong neodymium magnet rods which fit inside disposable plastic sleeves. Used for bead collection and transfer from the first well of larger cartridges. 2.5 Drawer Features 丨07 Apostle, Inc...
- Page 11 . There are tw o versions: one for 2 mL cartridges, and one for 8 and 15 mL. The instrument comes with a set of three of the same kind. 2.8 8/15 mL reagent rack 丨08 Apostle, Inc...
- Page 12 丨Summary 3丨Labware Description Image 2mL cartridge 8mL cartridge 15mL cartridge Reagent/Eluate Tube 1000 µL Pipette Tip Magnet Rod Sleeve Sample Tube 丨09 Apostle, Inc...
- Page 13 丨 Installation and site conditions Apostle will send an installation engineer to install the instrument. Please wait until they arrive to unpack the instrument and check the packing list. 1丨Installation Site Requirements ● Indoor use only ● Room temperature 20-30°C (68-86°F) during operation ●...
- Page 14 ● Ambient temperature: -20 to 55°C (-4 to 131°F) ● Relative humidity: <80% ● Atmospheric pressure: 500 to 1060 millibars ● Locate the instrument in a well-ventilated area, away from corrosive substances. 丨11 Apostle, Inc...
- Page 15 丨 Extraction process outline 1丨2 mL cartridge extraction process Step Description Illustration Lysis Incubation and lysis Mixing Binding Bead collection using pipette tip. Mixing and bead Wash transfer 丨12 Apostle, Inc...
- Page 16 丨 Extraction process outline Step Description Illustration Mixing Bead collection Elution Discarding beads Product recovery 丨13 Apostle, Inc...
- Page 17 丨 Extraction process outline 2丨5, 15, or 20 mL cartridge extraction process Illustration Step Description Lysis Incubation and lysis Mixing using the pipette tip Binding Bead collection using the magnet rod Mixing and bead Wash transfer 丨14 Apostle, Inc...
- Page 18 丨 Extraction process outline Step Description Illustration Mixing Bead collection Elution Discarding beads Product recovery 丨15 Apostle, Inc...
- Page 19 Turn on the instrument and log in. The default password for the Admin account is 0000. After changing your password, write it down and keep it safe. You will need to contact Apostle if you lose your password. To log out or switch users, press the user icon in the upper left corner.
- Page 20 The step name cannot be customized. Wait Set the program to either wait for a set time, or pause indefinitely until the user manually unpauses. Pierce Pierces the seal on pre-packaged reagent cartridges. For 2 mL cartridges only. 丨17 Apostle, Inc...
- Page 21 Mixing starts immediately, while the heating block is coming to temperature. Heating/Cooling sync Mix time starts counting after the set temperature is reached. Preheating /Precooling Mixing starts when the heating block reaches the set temperature. 丨18 Apostle, Inc...
- Page 22 Number of times the whole bead collection process is repeated. After you finish editing, save the protocol, or press “Save as” to create a copy under a different name. If you don’t want to save changes, press the back arrow. 丨19 Apostle, Inc...
- Page 23 HEPA filter are under the Sanitize tab. Users can start a timed sterilization and set an optional reminder to sterilize after each run. By default, the HEPA filter runs while the protocol is running. Alternatively, the HEPA filter can be turned on and off manually. 丨20 Apostle, Inc...
- Page 24 One-Click restore will permanently delete all user information, including all saved protocols and logs. Click on the current user in the upper left corner to switch users, change passwords, and manage user accounts. 丨21 Apostle, Inc...
- Page 25 3丨Annual Maintenance Contact the vendor for annual maintenance including the following: Alignment of extraction parts with the table surface Pipette maintenance, including O-ring, air tightness, and oil checks Pipette calibration Mechanical system checks Electrical system checks 丨Apostle, Inc 丨22...
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