Page 1 ® NitriTox Online Nitrificants Toximeter Operation Manual ® Document NitriTox -04E5114 12/14 Software - Version: 4.4.
Page 2 LAR Process Analysers AG. Changes which are used for technical improvement remain reserved.
Page 3: Table Of Contents
3.4.3 Nutrient solution pump ..............14 3.4.4 Flow Diagram ................15 3.5 Front view ....................16 How to Work with NitriTox® ................17 4.1 Start-up check list ..................17 4.2 Operating software ..................19 4.2.1 Starting................... 19 4.2.2 General remarks concerning the operating software ..... 22 4.2.2.1 Access right / operator levels ..........22...
Page 4 4.4.12 Limit settings ................46 4.4.13 Data base..................47 4.4.13.1 Saving data ................47 4.4.13.2 Measurement data as a table ..........49 4.4.13.3 Measurement data as a curve ..........50 4.4.14 Log-book ..................52 4.4.14.1 Saving log-book data ............54 ® Operation manual NitriTox...
Page 5 8.4.3.1 Measurement cell ..............95 8.4.3.2 Oxygen sensor ................96 8.4.3.3 Lid of fermentation vessel ............97 8.4.3.4 Aeration of the fermentation vessel and position of the BMR .98 8.4.3.5 Aeration stone and filterballs ...........99 8.4.3.6 Growth powder unit ..............100 ® Operation manual NitriTox...
Page 6 14 Safety data sheets of chemicals................131 14.1 Generally ....................131 14.2 N-Allylthiourea ATU ................132 14.3 Sodium hydroxide .................141 14.4 Edisonite Super ................150 14.5 Growth powder ...................154 14.6 Bacteria culture ..................156 14.7 Hydrochloride acid ................158 14.8 Potassium phosphate ................168 ® Operation manual NitriTox...
Page 7 Content 14.9 Di sodium hydrogen phosphate .............173 14.10 Borax (Sodium tetraborate decahydrate) ..........177 14.11 Activated carbon ..................181 Index........................I ® Operation manual NitriTox...
Page 8 Content ® Operation manual NitriTox...
Page 9: Operating Principle
Nitrifying bacteria, similar as used in aerobic WWTP‘s, are used for measurements. Therefore: same sensitivity. • No toxification of used biomass - only a subset is being used for the measurement in a separate measurement cell. 1. test water = sample water and the aerated reference water (optional) ® Operation manual NitriTox...
Page 10: What Affects The Measurement Of Toxicity
What affects the measurement of toxicity Fig. 1: Influencing factors on the measurement of toxicity ® Operation manual NitriTox...
Page 11: 1.3 Application
Protection of the nitrification process in a sewage treatment plant • Laboratory application ® The patented NitriTox quickly recognizes the effects of pollutants on sensitive bacteria (nitrifiers), allowing the operator to early take counteractions. A permanently self-regenerating bacteria culture of nitrifiers that is contained within the analyser allows the examination of water for dangerous ingredients within minutes.
Page 12 1 Operating Principle ® Operation manual NitriTox...
Page 13: Operational Control
Operational Control Fig. 2: Front view of the control panel Legend: 1) Touch screen 2) USB 2.0 interface ® Operation manual NitriTox...
Page 14 2 Operational Control ® Operation manual NitriTox...
Page 15: Preparing To Start
Wipe away overflown fluid from the cultivation vessel, the measuring cell or leaking tube connections immediately! ® , from the cultivation • Do never suck up or pipette by mouth any fluids from the tubes of NitriTox vessel or from the measuring cell! ® Operation manual NitriTox...
Page 16: Reagents
Top up the container to 10 l using demineralized water (Cl free tap water). Note: For different application, an other cleaning solution may be better. For that please contact the LAR technical support (chapter 10.2, page 111). ® Operation manual NitriTox...
Page 17: Atu - Solution
To prepare a 0.1 mol/l hydrogen chloride solution, 8 ml of 25 % hydrogen chloride needs to be mixed with in 1 l distilled water. These two buffer solutions can be stored in a refrigerator for approximately 3 weeks. 2. ATU = Allylthiourea C - a white crystalline compound - nitrification inhibitor ® Operation manual NitriTox...
Page 18: Filling The Fermentation Vessel
At this point of the start-up process, the pump for the dosage of nutrient solution will begin to work. From now on the pH value will stay constant and the measurement parameter "dosing" will start to increase. ® When a dosing rate of 160 -250 ml/h is established, the NitriTox is ready for a calibration and first measurements.
Page 19: 3.4 Tubing Installation
Please put the tubes in the tube pinch valves (chapter 3.4.1, page 12), tubing pumps (chapter 3.4.2, page 13) and nutrient solution pump (chapter 3.4.3, page 14). Please put the tubing into the respective pump head according to the following guidelines of the next pages. ® Operation manual NitriTox...
Page 20: Putting The Tubing Into The Tube Pinch Valves
5) Relieve the valve piston. Fig. 3: Putting the tube into the pinch valves Note: The solution will flow back into the canister, if the tubes are not fitted correctly in the pinch valves. ® Operation manual NitriTox...
Page 21: Peristaltic Pump
Do not change the adjustments of the small wheels on each side of the pump head! They are preset for the specified tubing. Changing of the adjustments may cause a poor pump performance (please see → point 6.3) in chapter 5.3, page 66 ff.). ® Operation manual NitriTox...
Page 22: Nutrient Solution Pump
The tubing must not be tightened over the rotor in order to avoid a re- duction of the tubings cross-section and of the pumps delivery volume. Fig. 5: Inserting the pump tubing into the nutrient solution ® Operation manual NitriTox...
Page 23: Flow Diagram
Table 1: Key of the flow diagram Short Description Oxygen sensor Flow sensor pH sensor Solution vessel Pump Fluid sensor Air pump Biomass Refeed (BMR) HW 1 Measurement cell HW 2 Growth powder unit Fermentation vessel Critical nozzle / choke Valve ® Operation manual NitriTox...
Page 24: Front View
3 Preparing to Start Front view Fig. 7: Overview of the analyser (look into the analyser) ® Operation manual NitriTox...
Page 25: How To Work With Nitritox
1) Check if drain for all liquids is present Fig. 6:, page 15 chapter 9.1, page 101 ff. ® 2) if existing: check if sample inlet of NitriTox ® connected to the FlowSampler (optional) 3) Check if nutrient solution is prepared chapter 3.2.1, page 8...
Page 26 4 How to Work with NitriTox® Fig. 8: Status screen (example) ® Operation manual NitriTox...
Page 27: 4.2 Operating Software
® Fig. 9: Power switch of the NitriTox On the first starting up, the toximeter analyser will go into offline mode (standby). After an electrical power cut or switching off, the analyser will go back to its last mode (online or standby), after it is powered on again.
Page 28 4 How to Work with NitriTox® Fig. 10: Login-window Fig. 11: Introductory screen with analyser serial number and software version ® Operation manual NitriTox...
Page 29 Fig. 12: Online button Note: After pressing the green “online“ button the respirometer will change to the measuring mode. To can- cel a measurement or any other running operation press the red “offline“ - button below. ® Operation manual NitriTox...
Page 30: General Remarks Concerning The Operating Software
4 How to Work with NitriTox® 4.2.2 General remarks concerning the operating software It is not necessary to memorise any commands to operate the toximeter. The toximeter offers a variety of functions on a screen to choose from. 4.2.2.1 Access right / operator levels There are two user levels with different access rights (see Fig.
Page 31 4.2 Operating software Fig. 14: Menu system - level II To enter any screen shown above please push the related function button. Fig. 15: Keyboard and keyboard button ® Operation manual NitriTox...
Page 32: What Happens During The Measurement
4 How to Work with NitriTox® What happens during the measurement? Fig. 16: Measuring signal in case of toxic event (example) Step 1: The present aerated test water or sample is filled into the measuring cell and optionally aerated. Step 2: The flow into the measurement cell stops.
Page 33 ® demineralized, aerated water (reference measurement). The NitriTox allows adjustable calibration frequencies ranging from 1 to 4 calibrations a day. The user may choose the calibration should include the measurement of a toxic ATU - solution.
Page 34: Toximeter Structure
4 How to Work with NitriTox® 4.3.1 Toximeter structure ® NitriTox basically consists of 5 assembly groups (see Fig. 18:) • Measuring cell for the sample measurement • Fermenter with nitrifiers (biomass) • Biomass refeed unit (BMR) • Growth Powder unit •...
Page 35: 4.4 Configuration
Some values are set by LAR technical support or authorised personnel. All values depend on the ap- plication and have to be adapted to the application. To change the values of the parameters, please contact the LAR technical support (chapter 10.2, page 111). ® Operation manual NitriTox...
Page 36: Measurement Parameter
4 How to Work with NitriTox® 4.4.2 Measurement parameter In this menu, which is available in level II, all measurement parameters can be set. After setting all measurement parameters, the measurement can be started with the green “online“ button. Three screens show the measurement values: •...
Page 37: Check-Interval
The sample can be diluted with reference water in the first measurement phase. If “yes“ is selected, the dilution will be calculated in the first measurement phase and taken into account in the toxicity measure- ment. For this setting please contact the LAR technical support (chapter 10.2, page 111). Default: ® Operation manual NitriTox...
Page 38: Amount Of Sample In Measurement Phase 1
4 How to Work with NitriTox® 4.4.2.8 Amount of sample in measurement phase 1 This parameter is for diluting the sample. For the dilution two parameter must be set. • Calculate amount of sample in measurement phase 1 • Amount of sample in measurement phase 1 Example: 50 % amount of sample in measurement phase 1, effects a dilution of 1:1.
Page 39: Minimal O2 Concentration
This setting is for the periodic addition of the growth powder solution. The number to be set is the number of times per day. Default: 4.4.2.18 Growth powder dosage time This parameter is the duration of the dosage in seconds. Default: 5 sec ® Operation manual NitriTox...
Page 40: Rinsing Parameters
4 How to Work with NitriTox® 4.4.3 Rinsing parameters Fig. 20: Rinsing parameter (default values) 4.4.3.1 Rinsing volume after measurement Here the volume of reference water is set that will be used for rinsing after each measurement. Minimum volume: 100 ml...
Page 41: Backwashing Of Tubes After Measurement
If the parameter “backwashing of tubes after the measurement“ is set on “Yes“, this parameter needs to be set too. The longer the tubes are the longer the set time should be. Minimum time interval: 5 sec Maximum time interval: 30 sec Default: 20 sec ® Operation manual NitriTox...
Page 42: O2 Sensor Calibration
4 How to Work with NitriTox® 4.4.4 Sensor Calibration During online mode the oxygen sensor will be re - calibrated automatically in regular time intervals. Frequency may be adjusted in menu measurement parameters (chapter 4.4.2.11, page 30). The "manual" calibration should be carried out when: •...
Page 43: Atu Consumption
6.0. Default: 4.4.4.9 Calibration oxygen sensor Press this button to start the calibration process of the oxygen sensor. 4.4.4.10 Oxygen sensor plot Here the signal can view during the O calibration. ® Operation manual NitriTox...
Page 44 4 How to Work with NitriTox® Meanwhile, the signal of the calibration can be seen in a new screen "O sensor calibration plot" , for this push the button "oxygen sensor plot“. The oxygen consumption of the aerated non toxic reference water, is monitored and compared to this value (>0.4 mg/l).
Page 45: Ph Calibration
® The monitoring of the pH value in the fermentation vessel is essential for the operation of the NitriTox Due to the nitrification the pH value changes more and more into the acid area. For compensation of this effect, nutrient solution is added in suitable manner while the pH value is measured constantly with a pH sensor.
Page 46: Measurement Interval
4 How to Work with NitriTox® 5) Press the button "reset calibration", so that "calibrate pH 7“ is active. Note: When the analyser has been started up for the first time all 3 buttons (calibration pH 7, calibration pH 8 and reset calibration) are active! 6) Put the sensor in the buffer solution pH 7 for 60 seconds.
Page 47: Relay Setting
Program one of the 4 relays according to "M1" (without the quotation marks). This sequence means that the relay is closed after completion of the sample stream. This contact remains closed until the beginning of the next measurement. ® Operation manual NitriTox...
Page 48: Relay Testing
4 How to Work with NitriTox® 4.4.6.2 Relay testing There are two ways to check the relays: • Check with a multi meter (only an authorized person should carry out this operation, because the rear housing must be opened) or •...
Page 49: Pc Settings
(see Fig. 15:, page 23). • type in the new password (making note of upper- and lower case letters) in the field. • confirm this using the “floppy disc“ symbol (chapter 4.5.7, page 59). ® Operation manual NitriTox...
Page 50: Measurement Value Screen
4 How to Work with NitriTox® 4.4.8 Measurement value screen The measurement screen shows the current measurement value and the time it was measured. The dosing rate of the nutrient solution is also shown on this screen. Fig. 26: Measurement value screen (example) Note: The modules „measurement value screen“...
Page 51: 24 H - Profile
The modules „measurement value screen“ and „24 h- Profile“ that are shown in menu level I are only available when a measurement is carried out. When the measurement is stopped, these modules are greyed out and the module „introductory screen“ will be activated. ® Operation manual NitriTox...
Page 52: Signal Curve
4 How to Work with NitriTox® 4.4.10 Signal curve To view the measurement curve of the actual measured value, go to the screen “signal curve“. On the left side additional level dependent data is given. For level II users the recent O concentration [mg/l] for the actual measurement is visible.
Page 53: Status Screen
Errors • Limits • Control state Note: • When the device is offline then "offine" is simply displayed at the position "next measurement". • Next auto calibration can be only possible when the device is online. ® Operation manual NitriTox...
Page 54: Limit Settings
4 How to Work with NitriTox® 4.4.12 Limit settings 4 limits can be adjusted. To set one limit, the following settings have to be done: • choose: the parameter (toxicity or dosage) • enter: the minimum value of the measurement value (lower limit) •...
Page 55: Data Base
1) Connect the USB stick to the analyser (Fig. 2:, page 5). 2) Choose the desired channel data.The time interval must be set/ taken into account. 3) Press the “save“ button (number 4 in Fig. 55:) and the data will be copied to the USB stick. ® Operation manual NitriTox...
Page 56 4 How to Work with NitriTox® Fig. 31: Data base - calender (example) ® Operation manual NitriTox...
Page 57: Measurement Data As A Table
Only the last 200 curves are saved, thus it is possible that older signal curves can not be drawn up. ® Operation manual NitriTox...
Page 58: Measurement Data As A Curve
4 How to Work with NitriTox® 4.4.13.3 Measurement data as a curve If the chosen measurement data on the screen “main represention of the data“ (no. 1 in Fig. 30:, page 47) should be shown as a curve, then the “measurement data as curve“ button has to be activated (no.
Page 59 4.4 Configuration Fig. 34: Data base - O2 sensor details - plot (example toxicity) ® Operation manual NitriTox...
Page 60: Log-Book
4 How to Work with NitriTox® 4.4.14 Log-book When logged in as user with level II rights, the log-book can be accessed via the status bar (Fig. 35:). Use the symbol “go to the log-book“ (no. 2 in Fig. 35:) and a selection window will pop up. In this window, touch the “control state“...
Page 61 Press the button "set" located in the bottom right of the screen when finished. The requested parameter (example: errors) will show here for the selected time. Fig. 36: Log-book (example) Fig. 37: Go to the log-book (example) ® Operation manual NitriTox...
Page 62: Saving Log-Book Data
4 How to Work with NitriTox® 4.4.14.1 Saving log-book data Log-book data can be saved to an USB stick: 1) Connect the USB stick to the analyser (Fig. 2:, page 5). 2) Choose the desired data.The time interval must be set/ taken into account (chapter 4.4.13, page 47 ff.).
Page 63: Data Storage
A backup of the parameters obtained during the installation process is stored for safety reasons and archived in the LAR - Database. If needed, please get in touch with LAR technical support to get the stored data (chapter 10.2, page 111). ® Operation manual NitriTox...
Page 64: Analog Out Calibration
4 How to Work with NitriTox® 4.4.16 Analog out calibration In this menu, the operator can decide, whether he wants to use a 0 - 20 mA or 4 - 20mA signal for his analog outputs. If 4 - 20 mA is chosen, the “life-zero“ melanism is used. This means that in case of an error, 0 mA can exported out on the electrical output (analog output).
Page 65: 4.5 Important Tasks, Good To Know
(see chapter 4.4.7, page 41 ff.). 4.5.5 Language The software language of the analyser is set by LAR technical support. To change the language, please contact LAR technical support (chapter 10.2, page 111) or an authorized distributor. ® Operation manual NitriTox...
Page 66: To Zoom In Diagrams
4 How to Work with NitriTox® 4.5.6 To zoom in diagrams Some screens (e.g. signal curve) have diagrams. The curve can be seen closer (zoomed). For that, cre- ate a box in the screen (Fig. 39:) with the finger or a touch pen. Now click inside of the box and the cutting box will be seen over the whole screen (Fig.
Page 67: Save
If the first parameter is wrong, deactivate this parameter with the “hook“ next to the parameter. Then the saving process can be finished with the green “hook“. The first parameter won‘t change but the other four are changed. ® Operation manual NitriTox...
Page 68: The Status Identification
4 How to Work with NitriTox® The status identification The current status of operations is reported in the status screen (Fig. 8:, page 18). This screen will only display the latest status reports, current errors and limit messages. For a status report per day (24 h - profile) please enter the “log-book“(chapter 4.4.14, page 52 ff.).
Page 69 Air flow fallen short of Nozzles (holes in the fer- Check aeration menter ground) blocked or compressor/choke broken E1991 Exceeded air flow Tubes between air flow sen- Check aeration sor and check valve to the choke interrupted ® Operation manual NitriTox...
Page 70 4 How to Work with NitriTox® ® Operation manual NitriTox...
Page 71: Control, Maintenance And Service
A detailed description of maintenance and service tasks is shown inchapter 5.3, page 66 ff. and chapter 5.4, page 74 ff.. Explanation of abbreviations: Daily Weekly Monthly Every 3 months Every 6 months Yearly C&M Control & Maintenance Service (change of worn parts and consumables) ® Operation manual NitriTox...
Page 72 7.4) Replacing the valve tubes and pump tubes 7.5) Checking tube system / removing the sediments / C&M replacing 8) Measurement cell 8.1) Checking the dosage rate of the biomass into the meas- C&M urement cell 8.2) Checking the measurement cell C&M ® Operation manual NitriTox...
Page 73 ® • Wash your hands after working on NitriTox If you have problems with the maintenances and service (if any part or value looks questionable or you are not sure about the correctness), please contact a authorized personnel by LAR or the LAR technical support (chapter 10.2, page 111).
Page 74: Description Of The Individual Control And Maintenance Task
If necessary: remove liquid from the fermenter by aid of a syringe and wash out sediments or remove them with an appropriate brush (e.g. bottle brush). ® Operation manual NitriTox...
Page 75 Is enough solution in the different canisters (nutrient solution, cleaning solution, reference water)? If the solutions need to be filled up, please prepare a new one and if necessary clean the canister. See → point 5.2). ® Operation manual NitriTox...
Page 76 LAR technical support (chapter 8.2, page 73). After this task, the measurement can be started again. Note: Please make sure that pumps are switched off. Risk of injury! ® Operation manual NitriTox...
Page 77 The pump should be set to the above-named value, as any deviating value can cause low pump ca- pacity, following into incorrect results. After the maintenance, the pumps need to be switched on again. After this task, the measurement can be started again. ® Operation manual NitriTox...
Page 78 (optional), a sink or any other pressureless drain. The tubes can not be extended ® outside of NitriTox . For the drain tube of the settling vessel it is also important, that any possibility of a backlog of the liquid or an insufficient gradient of the drain needs to be avoided.
Page 79 BMR lid and draw the biomass liquid into the syringe. Hold the full syringe at the edge of the vessel and let liquid run out. Alternatively you can use the bottle brush. After this task, the measurement can be started again. ® Operation manual NitriTox...
Page 80 If the vessel is dirty, you can take it out and clean it. Only clean it using purified water and a bottle brush. After cleaning the vessel, tube it and fill it with new growth powder solution. After this task, the measurement can be started again. ® Operation manual NitriTox...
Page 81 • clean sample tubing with pipe cleaner. • if necessary, replace tubing to the analyser. • reconnect all connections. • open sample inlet of your process. After this task, the measurement can be started again. ® Operation manual NitriTox...
Page 82: Description Of The Service Task
A new stone can be installed. After this task and the fermenter lid with cleaned or exchange aeration stone, the measurement can be started again. Note: Use gloves and safety goggle for that service task. ® Operation manual NitriTox...
Page 83 After that, close the clip at the tube A (Fig. 45: on page 75) and fill the biomass back into the fermentation vessel. Little screw for fixing the fermenter lid Fig. 45: Fermenter vessel and BMR unit After this task, the measurement can be started again. ® Operation manual NitriTox...
Page 84 See chapter 3.4.2 on page 13 and chapter 3.4.3 on page 14. After this task, the measurement can be started again. 7.5) Checking tube system / removing the sediments / replacing See → point 7.5) in chapter 5.3, page 66 ff.. ® Operation manual NitriTox...
Page 85 See → point 9) in chapter 5.3, page 66 ff.. 10) Growth powder unit See → point 10) in chapter 5.3, page 66 ff.. 11) FlowSampler® (optional) See → point 11) in chapter 5.3, page 66 ff.. ® Operation manual NitriTox...
Page 86 5 Control, Maintenance and Service ® Operation manual NitriTox...
Page 87: Optimisation
Optimisation For further instructions or wishes, please contact LAR technical support (chapter 10.2, page 111). ® Operation manual NitriTox...
Page 88 6 Optimisation ® Operation manual NitriTox...
Page 89: Troubleshooting
After a power failure, an automatic start of the analyser is carried out if the analyser was in measurement mode at the time of the power failure. The auto start can be stopped with the red “offline“ button. After the auto start, the analyser will continue with the measurement. ® Operation manual NitriTox...
Page 90: Interpreting Failure Indications Correctly
This message appears when the oxygen concentration has dropped below a certain minimum value. This means that the supplied sample is insufficiently aerated, so that the measured values must be dropped by. ® In the following table the indications of NitriTox and the remedies are comprised: Table 6: Indications and remedies Indication...
Page 91: 7.3 Problems During The Recording Of Measured Values
Please check, whether the ambient temperature is within the permissi- conditions: ble range of 5 - 35 °C (41 - 95 °F). Call your local service or the LAR technical support (chapter 10.2, page 111) - we gladly advise you! ® Operation manual NitriTox...
Page 92 7 Troubleshooting ® Operation manual NitriTox...
Page 93: Installation
Relative humidity should be max. 80%. • Direct sun light should be avoided. • Power supply: 230 V or 115 V/ 50 Hz, max. 1000 VA. • Ambient air should be clean, otherwise contamination gets into the fermenter. ® Operation manual NitriTox...
Page 94: Mounting
Usually the analyser is directly mounted to a wall (Fig. 47:, page 87). It is also possible to order a mounting rack (chapter 8.2.2, page 88 ff.) to attach the analyser to. ® Fig. 46: NitriTox ® Operation manual NitriTox...
Page 95: Wall Mounting
No concrete walls are inappropriate for the assembly. Please use four armature bolts for the analyser’s wall mounting. ® Fig. 47: NitriTox Note: Our LAR support does not carry out any building or mounting work. The mounting of the analyser and specific options are to be overseen by the customer.
Page 96: Mounting Rack
Fig. 48: shows the pre-assembled mounting rack. Fig. 48: Pre -assembled mounting rack Install the cross braces and adjustable feet on the mounting rack. Fig. 49: Assembly of mounting rack ® Operation manual NitriTox...
Page 97 Then the lower screws are inserted and all screws fastened. ® on mounting rack Fig. 50: NitriTox ® Operation manual NitriTox...
Page 98: Removing Transportation Locks
Cable for heat/cool trans- The cable is packed in a bubble fer mechanism wrap and fixed on the parting plane. Remove the bubble wrap and install the cable with the heat/cool transfer Fig. 52: Parting plane mechanism. ® Operation manual NitriTox...
Page 99: 8.4 Connections
Please check the local power supply meets the requirements in accordance with the label on your analyser before switching the analyser on. If the voltage is not correct, please contact LAR technical support (chapter 10.2, page 111). ® Operation manual NitriTox...
Page 100: Electrical Connections
Digital inputs used by customer (remote control) used by customer (remote control) fluid sensor fluid sensor fluid sensor ® Operation manual NitriTox...
Page 101: 8.4.2.1.1 Digital Input Line
20 mA a so-called life-zero feature may be initiated in the operating software meaning that instrument failures are assigned to the 0 mA level. The conditions for the failure indication can be individually pro- grammed with the operating software. ® Operation manual NitriTox...
Page 102: 8.4.2.4 Serial Interface Rs232
"E4124_E1990_P", "L1_toxicity_L2_dosing" or "E1870_L1_toxicity_M1" (the underlines here and in the following represent blank space characters). Different activities are able to be performed in the status ring (table 3, page 60). The maximum length of the character string is 4095. ® Operation manual NitriTox...
Page 103: Installation Of Individual Components
8.4 Connections 8.4.3 Installation of individual components 8.4.3.1 Measurement cell The small stirrer bar (20x6mm) is inserted into the measuring cell. measuring cell stirrer bar Fig. 56: Measuring Cell with small stirrer bar ® Operation manual NitriTox...
Page 104: Oxygen Sensor
1) Inlet: sample, reference water, cleaning solution or ATU and air 2) Inlet: biomass 3) Outlet: drain 4) Oxygen sensor 5) Temperature sensor Fig. 57: Correct position of the screw joint (oxygen sensor) in the measuring cell ® Operation manual NitriTox...
Page 105: Lid Of Fermentation Vessel
1) pH - sensor position 2) Nutrient solution 3) Biomass refeed (BMR) 4) Aeration stone 5) Lid of the fermentation vessel 6) Growth powder suspension air elevation mechanism Fig. 58: Connection in the lid of the fermentation vessel ® Operation manual NitriTox...
Page 106: Aeration Of The Fermentation Vessel And Position Of The Bmr
The BMR unit is able to push valuable biomass back into the fermenter vessel on a regular base. Legend: 1-4: Aeration points 5: Biomass refeed (BMR) Fig. 59: Installation aeration of the fermentation vessel ® Operation manual NitriTox...
Page 107: Aeration Stone And Filterballs
For the transport, the aeration stone is dismounted from the fermenter vessel lid. After the nirtrifying bacteria are inside of the fermenter vessel with the filterballs, the aeration stone can be put in the fermentation vessel lid. Fig. 60: Aeration stone Fig. 61: Filterballs ® Operation manual NitriTox...
Page 108: Growth Powder Unit
3.2.4 on page 9. Legend: 1) Growth powder unit (housing) 2) Inlet into the fermentation vessel 3) Vessel with growth powder solution 4) Stirrer Fig. 62: Growth powder unit ® Operation manual NitriTox...
Page 109: Accessories And Optional
Accessories and Optional ® The options for the NitriTox are: ® • FlowSampler • External aeration vessel • Activated carbon cartridge for aeration • mounting rack and ® • FlowSampler , external aeration vessel, activates carbon cartridge ® FlowSampler ®...
Page 110 The sample dosing can be done by an immersion pump which may require a cutting device, depending on sample composition. The pump should has to guarantee the necessary flow of 2-5 m³/h (max. 10 m³/ h) over the bridged distance. ® Operation manual NitriTox...
Page 111: Putting Into Operation
9.1 FlowSampler® 9.1.1 Putting into operation 1) Establishing all connections 2) Open the flow (sample inlet) 9.1.2 Maintenance Please see chapter 5.3, page 66 ff. for the maintenance work. ® Operation manual NitriTox...
Page 112: External Aeration Vessel
The additional aeration vessel is placed at the left wall of the device. The additional aeration can be activated or deactivated in the measurement parameters (see chapter 4.4.2, page 28 ff.). External aeration vessel Fig. 64: ® Operation manual NitriTox...
Page 113 Table 11: Key of the flow diagram Short Description Oxygen sensor Flow sensor pH sensor Solution vessel Pump Fluid sensor Air pump Biomass Refeed (BMR) HW 1 Measurement cell HW 2 Growth powder unit Fermentation vessel Critical nozzle / choke Valve ® Operation manual NitriTox...
Page 114: Maintenance And Service
Pull off the tubes and then the caps of the vessels are taken off. The vessels can be cleaned using a bottle brush. After cleaning, the vessels should be rinsed with 200ml ultrapure water. After cleaning, the vessel can be put back and the tubes can be reconnected. ® Operation manual NitriTox...
Page 115: Activated Carbon Cartridge For Aeration
(gas tubing) Standard Flüssigkeitsschläuche (liquids tubing) -HS 1 -CM 6 Erstellt: 10.09.14 Jechorek -EN ISO 10628 Geprüft: 10.09.14 F.Klaus Option 02 Option 01 Freigegeben: 10.09.14 Genthe Revision: Nummer: Flow diagram with activated carbon cartridge Fig. 67: ® Operation manual NitriTox...
Page 116: Maintenance And Service
A711580drw11 sieve A711580drw19 Filtermatte D=60 filter mat D=60 A581028 O-Ring 65x4 O-ring 64*4 P 583/NBR 70 Label: Schild "max 4bar" maximal 1 bar Filterpatrone vollst. cartridge Filterpatrone vormont. A711580drw10 Activated carbon cartridge (exploded view) Fig. 68: ® Operation manual NitriTox...
Page 117: Replacing Air Filter (3 Month)
The filter is located behind the front panel. Open the rear house. Having done so, the tube connections of the old filter can be pulled off. Then the new filter can be connected to the tubes. Once, the filter has been replaced, the rear house can be closed. Filter Fig. 70: ® Operation manual NitriTox...
Page 118 9 Accessories and Optional ® Operation manual NitriTox...
Page 119: Technical Information
10,4“ - screen, resistive TFT display (touch display) 10.2 LAR contact Table 13: LAR contact Contact Name Telefone Number E-Mail Adresse Technical Support +49 30 278958 - 55 service@lar.com Export +49 30 278958 - 43 export@lar.com ® Operation manual NitriTox...
Page 120: Site
<= 80% Mounting rack dimensions 2000 x 1000 x 815 mm (H x W x D); weight of rack: 65 kg ® FlowSampler (optional) dimensions Carrier panel: 500 x 400 x 120mm (H x W x D) ® Operation manual NitriTox...
Page 121: Flow Diagrams
Flow Diagrams ® Fig. 71: NitriTox ® Operation manual NitriTox...
Page 122 11 Flow Diagrams ® with external aeration vessel Fig. 72: NitriTox ® Operation manual NitriTox...
Page 123 11 Flow Diagrams ® with activated carbon cartridge Fig. 73: NitriTox ® Operation manual NitriTox...
Page 124 11 Flow Diagrams ® Operation manual NitriTox...
Page 125: Operators Log
Operators Log ® Operation manual NitriTox...
Page 126 12 Operators Log ® Operation manual NitriTox...
Page 127: Level Iii: For Trained Users Only
Level III and the necessary USB stick is only granted after a technical training with LAR. To do so please contact LAR technical support (chapter 10.2 on page 111). 13.2 Operating level III Fig. 74: Operating level III ® Operation manual NitriTox...
Page 128: Measurement Parameters (Level Ii)
If a dilution from the sample should be done, the parameter “calculate amount of sample in measurement phase 1“ must be activated (YES). For this setting please contact the LAR technical support (chapter 10.2, page 111). Fig. 75: Measurement parameter screen in level II as a user III (example) ® Operation manual NitriTox...
Page 129: Pc Settings (Level Ii)
The word “auto“ can be written in. That mean the software will find automatically the IP - address. If more information is needed, please contact LAR technical support (chapter 10.1 on page 111). entry (example): inet:_static_address:_10.0.2.15_netmask:_255.255.255.0_gateway:_10.0.2.1_nameserver:_10.0.2.1 ® Operation manual NitriTox...
Page 130: Analog Out Calibration (Level Ii)
Before checking the analog outputs make sure the analyser is in offline mode and no measurement is performed. Testing: ® • Select analog out number calibration: in NitriTox there is only one analog output (Analog OUT1) • Select “0-20 mA“ under life zero •...
Page 131: Signal Curve (Level Ii)
On this screen, in level II, the user level III can see a few more parameters for each measurement phase that the level II user cannot see: • signal start and end • temperature in measurement cell • consumption Fig. 78: Signal curve screen in level II as a user III ® Operation manual NitriTox...
Page 132: Can Selftest
The analyser has a so called CAN circuit diagram. All parts of the analyser are registered here and every part has its own ID. The analyser tests this plan independently and shows the results here. Fig. 79: CAN selftest ® Operation manual NitriTox...
Page 133: Digital In
13.2 Operating level III 13.2.6 Digital IN In these screen, the states of digital inputs 1 are shown. Fig. 80: Digital IN 1 ® Operation manual NitriTox...
Page 134: Digital Out 1
In these screen, the state of the digital outputs 1 (on/off) is shown and can be defined.Components of the analyser (example: valves) can be switched on. The functions are useful for troubleshooting or maintenance purpose. Fig. 81: Digital OUT 1 ® Operation manual NitriTox...
Page 135: Update - Manager
If you click on “accept“ the screen will shut down for 30-40 seconds and the new software will be installed. The yellow button with the arrow restarts the analyser with a soft reboot. Fig. 82: Update - Manager ® Operation manual NitriTox...
Page 136: Names And Units
13.2.9 Names and Units In this screen, the names, units and minimum and maximum of channels can be adjusted. The minimum and maximum is used to adjust the axis. Fig. 83: Screen „Names and Units“ (Example) ® Operation manual NitriTox...
Page 137: 13.2.10 Hardware Info
13.2 Operating level III 13.2.10 Hardware Info This screen shows information about the hardware of the analyser: • PC info • Network The network settings can be adjusted (Chapter 13.2.2.3 on page 121). Fig. 84: Screen „Hardware Info“ (Example) ® Operation manual NitriTox...
Page 138 13 Level III: For Trained Users Only ® Operation manual NitriTox...
Page 139: Safety Data Sheets Of Chemicals
The LAR AG does not guarantee the correctness or dateness of the safety data sheets. Please make sure to get the most safety data sheets from your chemical supplier. ® Operation manual NitriTox...
Page 140: N-Allylthiourea Atu
C4-H8-N2-S, H2C=CHCH2NHC(=S)NH2, "urea, 1-allyl-2-thio-", allylthiocarbamide, allylthiourea, N-allylthiourea, 1-allylthiourea, (2-propenyl)thiourea, thiocynamine, thiosinamin, thiosinamine, Aminosin, Rhodalline, U-19571 Section 2 - HAZARDS IDENTIFICATION CHEMWATCH HAZARD RATINGS Flammability Toxicity Min/Nil=0 Body Contact Low=1 Moderate=2 Reactivity High=3 Chronic Extreme=4 CANADIAN WHMIS SYMBOLS 1 of 9 ® Operation manual NitriTox...
Page 141 For advice, contact a Poisons Information Centre or a doctor. Urgent hospital treatment is likely to be needed. In the mean time, qualified first-aid personnel should treat the patient following observation and employing supportive measures as indicated by the patient's condition. 2 of 9 ® Operation manual NitriTox...
Page 142 Combustion products include carbon monoxide (CO), carbon dioxide (CO2), nitrogen oxides (NOx), sulfur oxides (SOx), other pyrolysis products typical of burning organic material. 3 of 9 ® Operation manual NitriTox...
Page 143 Material Notes mg/m³ mg/m³ mg/m³ F/CC 1-allyl-2-thiourea Canada - Ontario (Particles Occupational (Insoluble or 10 (I) Exposure Limits Poorly Soluble) Not Otherwise) Canada - British 1-allyl-2-thiourea Columbia (Particles 10 (N) Occupational (Insoluble or 4 of 9 ® Operation manual NitriTox...
Page 144 (Z-1) (f) Respirable federal Limits. PNOR Fraction) means “particles not otherwise regulated.” 1-allyl-2-thiourea US - Wyoming (Particulates not Toxic and otherwise Hazardous regulated Substances Table (PNOR)(f)- Z1 Limits for Air Respirable Contaminants fraction) 5 of 9 ® Operation manual NitriTox...
Page 145 Vapour Pressure (mmHG) Negligible Upper Explosive Limit (%) Not available. Specific Gravity (water=1) 1.110 Lower Explosive Limit (%) Not available Relative Vapour Density (air=1) Not Applicable Volatile Component (%vol) Negligible Evaporation Rate Not applicable 6 of 9 ® Operation manual NitriTox...
Page 146 Dispose of by: burial in a land-fill specifically licenced to accept chemical and / or pharmaceutical wastes or Incineration in a licenced apparatus (after admixture with suitable combustible material) 7 of 9 ® Operation manual NitriTox...
Page 147 Section 16 - OTHER INFORMATION LIMITED EVIDENCE ■ Inhalation and/or skin contact may produce health damage*. ■ Cumulative effects may result following exposure*. * (limited evidence). Denmark Advisory list for selfclassification of dangerous substances 8 of 9 ® Operation manual NitriTox...
Page 148 This document is copyright. Apart from any fair dealing for the purposes of private study, research, review or criticism, as permitted under the Copyright Act, no part may be reproduced by any process without written permission from CHEMWATCH. TEL (+61 3) 9572 4700. www.Chemwatch.net Issue Date: Jan-10-2010 Print Date:Feb-22-2012 9 of 9 ® Operation manual NitriTox...
Page 149: 14.3 Sodium Hydroxide
14.3 Sodium hydroxide 14.3 Sodium hydroxide ® Operation manual NitriTox...
Page 150 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 151 14.3 Sodium hydroxide ® Operation manual NitriTox...
Page 152 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 153 14.3 Sodium hydroxide ® Operation manual NitriTox...
Page 154 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 155 14.3 Sodium hydroxide ® Operation manual NitriTox...
Page 156 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 157 14.3 Sodium hydroxide ® Operation manual NitriTox...
Page 158: Edisonite Super
In adaptation to materials stored in the immediate neighbourhood. Special risks: Non-combustible. Special protective equipment for fire fighting: Do not stay in dangerous zone without self-contained breathing apparatus. Other information: Prevent fire-fighting water from entering surface water or groundwater. page 1 of 4 ® Operation manual NitriTox...
Page 159 Explosion limits lower not available upper not available Vapour pressure ( 20°C) not available Relative vapour density not available Density ( 20°C) ~ 800 kg/m³ Solubility in water ( 20°C) > 100 g/l page 2 of 4 ® Operation manual NitriTox...
Page 160 Recycling has to be preferred instead of disposal. Can be incinerated in compliance with local official regulations. Waste key number: 07 06 99 (EC) Packaging: Empty packaging may be treated like household waste or recycled. 14. Transport information Not subject to transport regulations. page 3 of 4 ® Operation manual NitriTox...
Page 161 The information contained herein is based on the present state of our knowledge. It characterises the product with regard to the appropriate safety precautions. It does not represent a guarantee of the properties of the product. page 4 of 4 ® Operation manual NitriTox...
Page 162: Growth Powder
, 8 $ 8 " - 3 : $ $ 7 < : 3 7 * ® Operation manual NitriTox...
Page 163 " " 6 $ 6 $ " , + $ +))( +))5 3 : $ $ 7 < : 3 7 * ® Operation manual NitriTox...
Page 164: Bacteria Culture
, 7 " - 3 : $ $ 7 < : 3 7 - ® Operation manual NitriTox...
Page 165 " " 6 $ 6 $ " 2 / + $ +))( +))5 3 : $ $ 7 < : 3 7 ® Operation manual NitriTox...
Page 166: Hydrochloride Acid
14 Safety data sheets of chemicals 14.7 Hydrochloride acid ® Operation manual NitriTox...
Page 167 14.7 Hydrochloride acid ® Operation manual NitriTox...
Page 168 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 169 14.7 Hydrochloride acid ® Operation manual NitriTox...
Page 170 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 171 14.7 Hydrochloride acid ® Operation manual NitriTox...
Page 172 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 173 14.7 Hydrochloride acid ® Operation manual NitriTox...
Page 174 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 175 14.7 Hydrochloride acid ® Operation manual NitriTox...
Page 176: Potassium Phosphate
14 Safety data sheets of chemicals 14.8 Potassium phosphate ® Operation manual NitriTox...
Page 177 14.8 Potassium phosphate ® Operation manual NitriTox...
Page 178 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 179 14.8 Potassium phosphate ® Operation manual NitriTox...
Page 180 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 181: 14.9 Di Sodium Hydrogen Phosphate
- Skin contact: Wash off thoroughly with soap and water. - Ingestion: Wash out mouth thoroughly with water. In severe cases obtain medical attention. 5. Fire-fighting measures Special risks: Not combustible. May evolve toxic fumes in fire. ® Operation manual NitriTox...
Page 182 Health, safety and environmental information: Melting temperature 240°C (decomposition) Density(g/ml) 1.53 Bulk density: ~0.88 g/ml Solubility in water Soluble (77 g/l, 20°C) pH value 8.7-9.3 (50g/l, 20°C) Flammability: Not combustible. Log P(o/w): -5.8 (calculated) 10. Stability and reactivity ® Operation manual NitriTox...
Page 183 14. Transport information Not subject to transport regulations. 15. Regulatory information Labelling according to EC directives Not classified as dangerous according to EC Directives. EC-No.: 231-448-7 Local Regulations 16. Other information Revision. Supersedes issue of 04/07/01 ® Operation manual NitriTox...
Page 184 14 Safety data sheets of chemicals Changes in Section : 6,12 Date of issue: 13/01/06 Date of print: 19/12/07 ® Operation manual NitriTox...
Page 185: Borax (Sodium Tetraborate Decahydrate)
Get medical aid. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk Ingestion: or water. Wash mouth out with water. Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If ® Operation manual NitriTox...
Page 186 Personal Protective Equipment Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face Eyes: protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. ® Operation manual NitriTox...
Page 187 Dispose of in a manner consistent with federal, state, and local regulations. Section 14 - Transport Information US DOT Shipping Name: Not regulated. Hazard Class: UN Number: Packing Group: Canada TDG Shipping Name: Not regulated as a hazardous material Hazard Class: UN Number: Packing Group: ® Operation manual NitriTox...
Page 188 -------------------------------------------------------------------------------- ® Operation manual NitriTox...
Page 189: 14.11 Activated Carbon
14.11 Activated carbon 14.11 Activated carbon ® Operation manual NitriTox...
Page 190 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 191 14.11 Activated carbon ® Operation manual NitriTox...
Page 192 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 193 14.11 Activated carbon ® Operation manual NitriTox...
Page 194 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 195 14.11 Activated carbon ® Operation manual NitriTox...
Page 196 14 Safety data sheets of chemicals ® Operation manual NitriTox...
Page 197: Index
Growth Powder .......100 Introductory screen ......20 Life-zero Feature ........93 Limits ..........46 Maintenance and Service tasks ......... 64 Measuring Cell ........96 Menu System ........22, Nutrient Solution Pump ........14 Operating Principle......1, Oxygen Respiration......1 Oxygen Sensor Calibration......... 34 ® Operation manual NitriTox...
Page 198 Index ® Operation manual NitriTox...

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