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Micromax HM 340 E Instruction Manual

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Rotary Microtome HM 340 E
ROTARY MICROTOME
HM 340 E
INSTRUCTION MANUAL
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
386 200 - English

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  • Page 1 Rotary Microtome HM 340 E ROTARY MICROTOME HM 340 E INSTRUCTION MANUAL MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...
  • Page 2 Rotary Microtome HM 340 E MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...

  • Page 3: Warranty

    Rotary Microtome HM 340 E CERTIFICATION MICROM certifies that this instrument has been tested and checked carefully. Its technical data was verified before shipment to be in accordance with the published specifications. The instrument complies with applicable international safety regulations.
  • Page 4 Rotary Microtome HM 340 E MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...

  • Page 5: Intended Use

    Rotary Microtome HM 340 E INTENDED USE Dear Customer, Thank you for buying this MICROM instrument. Before putting the instrument into operation, please read these operating instructions carefully to familiarize yourself with its proper operation and functions. Only skilled or specially trained personnel must operate the microtome, i.e.
  • Page 6 Rotary Microtome HM 340 E Intended Use Table of Contents Safety Precautions Part 1 Introduction Description of the Rotary Microtome HM 340 E Technical specifications HM 340 E Part 2 Operating Instructions Setting up the microtome Initial turn-on Cutting movement and retraction...
  • Page 7 Rotary Microtome HM 340 E 2-12 Knife carriers 2-12-1 Standard knife carrier N 2-12-2 Disposable blade carrier E 2-12-3 Knife carrier C 2-12-4 Knife carrier S 2-12-5 Readjusting the knife carriers N, E and C 2-12-6 Readjusting the knife carrier S...
  • Page 8 Rotary Microtome HM 340 E How to avoid malfunctions 4-4-1 Preparation of the specimen 4-4-2 Temperature of the specimen 4-4-3 Tightening the clamping screws 4-4-4 Selection of the knife 4-4-5 Adjustment of the knife 4-4-6 Cutting speed 4-4-7 Trimming Part 5...
  • Page 9 Rotary Microtome HM 340 E SAFETY PRECAUTIONS ATTENTION ! Please observe the following general precautions during operation of this instrument. Failure to comply with these precautions violates safety standards and the intended use of the instrument. MICROM GmbH is not liable for misuse of the instruments and failure to comply with basic safety requirements.
  • Page 10 Rotary Microtome HM 340 E HAZARD OF MALFUNCTION To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic environment. This means, that transmitters such as mobile phones must not be operated in their close vicinity.
  • Page 11 Rotary Microtome HM 340 E WARNING SIGNALS AND SYMBOLS The installation and routine use of the HM 340 E is easy and safe if the instructions in this manual are being observed. Note: Special instructions regarding operation of the instrument.
  • Page 12 Rotary Microtome HM 340 E Fig. 1 MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...
  • Page 13 The HM 340 E will cut sections in a range from 0,5 µm up to 100 µm. For the protection of knife and specimen, the instrument retracts the specimen at the end of the cut. If desired, the function <retraction> can be turned off.
  • Page 14 Rotary Microtome HM 340 E 1 - 2 TECHNICAL SPECIFICATIONS HM 340 E Microtome: Section thickness range ............0,5 - 100 µm Resolution: ............0,5 µm for 0,5 - 2 µm .................. 1 µm for 2 - 10 µm ................2 µm for 10 - 20 µm ................

  • Page 15: Initial Turn-On

    Rotary Microtome HM 340 E PART 2 OPERATING INSTRUCTIONS SETTING UP THE MICROTOME Unpack the microtome and remove the wrapping. On the lower side of the instrument towards the front and rear longitudinal axis, there are recessed grips to lift or carry the microtome. Do not...

  • Page 16: Cutting Movement And Retraction

    Rotary Microtome HM 340 E CUTTING MOVEMENT AND LOCKING AND UNLOCKING RETRACTION THE HANDWHEEL To start the cutting movement of the microtome, The handwheel is integrated into the housing. The turn the handwheel. As the specimen moves down, position of the handle corresponds to the vertical sectioning is carried out (cutting movement, fig.
  • Page 17 Rotary Microtome HM 340 E Fig. 5 The graduation of the section thicknesses is SETTING SECTION THICKNESS divided into 6 ranges: AND TRIMMING THICKNESS The required section and trimming thicknesses are range graduation set by means of the control knob (fig. 5.3), which up to 2µm...
  • Page 18 Rotary Microtome HM 340 E Fig. 6 Three different speeds for the coarse feed are SPECIMEN FEED available: 400, 800 and 1200 µm/s. To select or change the speed, press button <speed coarse 2-6-1 COARSE FEED AND SPEED feed> (fig. 6.5) until the required speed is shown...

  • Page 19: Trimming And First Cuts

    Rotary Microtome HM 340 E 2-6-2 TRIMMING AND FIRST CUTS 2-6-3 FINE FEED After the specimen and the knife are adjusted, After having adjusted knife and specimen as well further gradual feed for trimming can be carried as having trimmed the specimen, sectioning can be out using the function <trimming>.
  • Page 20 Rotary Microtome HM 340 E INDICATION OF CUTTING 2-7-3 REMAINING TRAVEL TO PROCESSES FRONT END POSITION In the lower line of the display information about This value shows the distance in microns, which is the sectioning status can be seen. Press the <roll left for sectioning.
  • Page 21 Rotary Microtome HM 340 E TURNING OFF THE FUNCTION <RETRACTION> If desired, the function <retraction> can be turned off. This can be achieved by pressing the buttons (fig. 6.5 and 6.8) on condition that the lower line of the display (fig. 6.1) is blank.

  • Page 22: Standard Specimen Clamp

    Rotary Microtome HM 340 E 2-10 SPECIMEN CLAMPING 2-10-2 STANDARD SPECIMEN CLAMP To clamp specimens, different specimen clamping The standard specimen clamp (fig. 8) is used for systems are available. With the orienting adapter it rectangular and square paraffin and plastic blocks.
  • Page 23 Rotary Microtome HM 340 E 2-10-3 INSERT FOR ROUND SPECIMENS, 2-10-4 FOIL CLAMP V-INSERT AND V-DISTANCE PIECE The foil clamp (fig. 10) is a clamping system for foils or thin specimens. To insert the specimen, loosen the three clamping screws (fig. 10.5) slightly and push the movable jaw (fig.
  • Page 24 Rotary Microtome HM 340 E 2-10-5 SEGMENT ARC AND UNIVERSAL SPECIMEN HOLDER The segment arc and universal specimen holder are highly suitable for the clamping of small specimens embedded in plastic (fig. 11). Fig. 11 The specimen is inserted in the holder (fig. 11.1) and clamped by means of the screw (fig.
  • Page 25 Rotary Microtome HM 340 E 2-11 ADAPTERS FOR SPECIMEN CLAMPING 2-11-1 ADAPTER, NON-ORIENTING This adapter serves for the non-orienting fastening NOTE! To avoid undesirable movement while of the specimen clamps directly on the cylinder adjusting the specimen, press the eccentric lever (fig.
  • Page 26 Rotary Microtome HM 340 E 2-11-4 READJUSTING SPECIMEN CLAMPS Frequent use of the clamping lever (fig. 12.1) can lead to the fact that the specimen clamps cannot be clamped optimally anymore. If the necessary readjustments are not carried out, it might even be...
  • Page 27 Rotary Microtome HM 340 E 2-12 KNIFE CARRIERS Hazard of hand injuries: Due to moving parts in connection also with the microtome knife, a danger area arises, which might lead to hand injuries in case of non-compliance with the safety features of the microtome and when disregarding the instruction manual.
  • Page 28 Rotary Microtome HM 340 E Fig. 14 MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...
  • Page 29 Rotary Microtome HM 340 E 2-12-2 DISPOSABLE BLADE CARRIER E If the clamping lever (fig. 15.8) is loosened, the Inserting the blade upper part of the knife carrier can be moved The disposable blade carrier E is designed to take 1 cm to the left or right side.
  • Page 30 Rotary Microtome HM 340 E Fig. 15 MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...
  • Page 31 Rotary Microtome HM 340 E 2-12-3 KNIFE CARRIER C Inserting the knife Moving the knife carrier on the dovetail guide To insert the knife, the clamping screws (fig. 16.10) must be unscrewed slightly so the knife can Loosen the clamping lever (fig. 16.5) on the left be pushed in from the side.
  • Page 32 Rotary Microtome HM 340 E Fig. 16 Fig. 17 shows schematically the angles on the cutting edge profiles of c- and d-knives. Fig. 17 MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...
  • Page 33 Rotary Microtome HM 340 E 2-12-4 KNIFE CARRIER S Inserting the knife Turning the carrier The knife carrier S is designed to take triangular Loosen the clamping screw (fig. 18.4) to move the glass knives, diamond and sapphire knives. These carrier to the left or right side on the socket up to knives are put into the knife insert (fig.
  • Page 34 Rotary Microtome HM 340 E Fig. 18 MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf 386 200 - English...
  • Page 35 Rotary Microtome HM 340 E 2-12-5 READJUSTING KNIFE CARRIERS N, E AND C Frequent use of the clamping levers can lead to the fact that the knife carriers cannot be clamped optimally anymore. If the necessary readjustments are not carried out, it might even be possible that the knife carriers cannot be clamped anymore.

  • Page 36: Storage Unit

    Rotary Microtome HM 340 E 2-13 SECTION WASTE TRAY WITH 2-15 BACKLIGHTING SYSTEM INTEGRATED ARM REST The section waste tray with integrated arm rest The adjustment between specimen and cutting surrounds the knife carrier area. It can easily be edges...

  • Page 37: Memory Function

    To facilitate routine work for the user, the rotary When the first-cut-position as well as the safety microtome HM 340 E is equipped with two distance have been stored, please proceed as functions for the fast and exact approach of knife follows: edge and specimen.
  • Page 38 Rotary Microtome HM 340 E 2-16-2 AUTOMATIC APPROACH SYSTEM The automatic approach system is used for the fast After this process, turn the handwheel and thus the and exact approach of the specimen (fig. 24.1) specimen holder into the upper position. Then the towards the knife edge (fig.
  • Page 39 Rotary Microtome HM 340 E If the button (fig. 6.9) is pressed, although the automatic approach system is not installed or not yet ready for work, the display (fig. 6.1) shows NO APPROACH. Again press button (fig. 6.9) to delete this information. Then continue working as usual without automatic approach system.
  • Page 40 Rotary Microtome HM 340 E 2-17 LARGE FIELD MAGNIFIER The large field magnifier (fig. 25.1) is for looking at section production. The light is connected to the power outlet with the power cord (fig. 25.2) and turned on and off with the switch (fig.
  • Page 41 Rotary Microtome HM 340 E Installing the magnifier on the microtome Please use the enclosed fastening elements to attach the magnifier to the microtome. Remove the front plastic caps from the upper side of the housing. If there are no drilled holes in the housing, it must be replaced with a new one with drilled holes.

  • Page 42: Standard Equipment

    Rotary Microtome HM 340 E 2-18 STANDARD EQUIPMENT The microtome HM 340 E is supplied with the following accessories: 1 Hex head wrench 6 mm 1 Cover 1 Para Gard 1 Instruction manual The knife carriers and specimen clamps are not included in the standard equipment.

  • Page 43: Optical Accessories

    Rotary Microtome HM 340 E V-distance piece 715320 Segment arc 715050 Universal specimen holder 715060 2-19-3 OPTICAL ACCESSORIES Large field magnifier, 230 V 760160 Large field magnifier, 120 V 760170 Stereomicroscope Stemi 2000 755210 Adapter for Stemi 2000 532090 KL 1500 with ring light...

  • Page 44: Additional Accessories

    Rotary Microtome HM 340 E Superlap blades (10 pieces) 152190 2-19-5 ADDITIONAL ACCESSORIES Fast freezing unit K-300 100 V, 50 - 60 Hz 770080 115 V, 60 Hz 770070 230 V, 50 Hz 770030 240 V, 50 Hz 770090 Section transfer system STS...
  • Page 45 SPECIMEN COARSE FEED AND TRIMMING FUNCTION The manual rotary movement of the handwheel of the Rotary Microtome HM 340 E is converted into After changing the specimen or moving the knife a vertical movement of the cylinder head which or knife carrier, it is necessary to adjust the carries the specimen holder.
  • Page 46 Rotary Microtome HM 340 E KNIFE CARRIERS The knife carriers are easy to use. They allow the microtome knives to be clamped and adjusted as needed. Depending on application, special knife carriers are available. MICROTOME WITH FAST FREEZING UNIT The fast freezing unit K-300 allows frozen sectioning with the specimen temperature as low as -45°C.
  • Page 47 Rotary Microtome HM 340 E PART 4 WORKING WITH THE MICROTOME PREPARATION AND SECTIONING INSTRUCTIONS ORIENTATION Hazard of hand injuries: Before sectioning, the specimens must be prepared Due to moving parts in connection also appropriately and embedded in suitable media.
  • Page 48 Rotary Microtome HM 340 E HOW TO AVOID MALFUNCTIONS 4-4-1 PREPARATION OF THE SPECIMEN 4-4-5 ADJUSTMENT OF THE KNIFE When preparing specimens, be sure that a suitable Take care to adjust the proper clearance angle of embedding medium, fixation, dehydration and the knife: 5 - 7°...

  • Page 49: Maintenance

    Rotary Microtome HM 340 E PART 5 MAINTENANCE Annual routine maintenance To secure section quality and functioning of the microtome, it is recommended that a routine maintenance be performed by a trained service technician once a year. Service contract MICROM offers a service contract which guarantees that your instrument is always in Fig.

  • Page 50: Cleaning And Care

    Rotary Microtome HM 340 E PART 6 CLEANING AND CARE CLEANING Care Cleaning intervals Remove the knife from the knife carrier. Clean it Cleaning of the microtome depends on how and keep it in a knife case. frequently the microtome is used. The more it is used, the more it will need to be cleaned.
  • Page 51 Rotary Microtome HM 340 E DISPOSAL OF THE INSTRUMENT AFTER FINAL SHUTDOWN After the final shutdown of the instrument, we recommend to contact a local recycling company for the disposal according to the national applicable regulations. To be applied in the countries of...

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