Benchmark Scientific Accuris Smart-Q 800 Operation Manual
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Benchmark Scientific Accuris Smart-Q 800 Operation Manual

Fluorometer
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Operation Manual
V1.1
This Product is for Research Use Only

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Summary of Contents for Benchmark Scientific Accuris Smart-Q 800

  • Page 1 Operation Manual V1.1 This Product is for Research Use Only...
  • Page 2 Please inspect the instrument as well as all included accessories when you first open the packaging. If you find anything damaged or missing, please contact Benchmark Scientific or your local distributor immediately. BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS PO Box 709...
  • Page 3 Safety Warning and Guidelines 1. Important information for safe use Users should have a clear understanding of how to use this instrument before operation, please read this manual carefully prior to operation. NOTICE: Please review this manual in its entirety before operating the Smart-Q™...
  • Page 4 Unplug the instrument immediately if any of the following occur, and contact Benchmark Scientific’s Service Department: ⚫ Liquid enters the Instrument. ⚫ The instrument is dropped or the outer shell damaged. ⚫ The functions of the instrument have obviously changed.

  • Page 5: Table Of Contents

    Smart-Q 800 Fluorometer Operation Manual Chapter 1 Introduction Table of Contents Chapter 1 Introduction ......................3 Chapter 2 Specifications ......................4 1. Required Installation Environment ................4 Environmental Temperature: 10°C～40°C ................. 4 2. Specifications ....................... 4 Chapter 3 Instrument Overview ..................... 5 Chapter 4 Installation ......................
  • Page 6 Smart-Q 800 Fluorometer Operation Manual Chapter 1 Introduction Settings Interface ..................... 20 5.7.1 System Settings Interface .................. 20 5.7.2 Upgrade Interface ....................21 5.7.3 Maintenance Interface ..................21 Chapter 6 Troubleshooting ....................22...

  • Page 7: Chapter 1 Introduction

    Smart-Q 800 Fluorometer Operation Manual Chapter 1 Introduction Chapter 1 Introduction The Smart-Q™ 800 Fluorometer is used for the precise quantitation of nucleic acids and proteins in microliter volumes via fluorescent detection. Under conditions of low concentrations of molecules, concentration values are linear to fluorescence intensity.

  • Page 8: Chapter 2 Specifications

    Smart-Q 800 Fluorometer Operation Manual Chapter 3 Instrument Structure Chapter 2 Specifications 1. Required Installation Environment Environmental Temperature: 10°C～40°C Relative Humidity: ≤ 70% Input Voltage: 24 VDC 2A 2. Specifications Model Smart-Q™ Fluorometer Display 7.0-inch, color touch screen Dynamic Range 4 orders of magnitude Blue LED Light Source...

  • Page 9: Chapter 3 Instrument Overview

    Smart-Q 800 Fluorometer Operation Manual Chapter 3 Instrument Structure Chapter 3 Instrument Overview This chapter provides an overview of the physical characteristics of the Smart-Q 800. When using the instrument for the first time, please read this chapter carefully to familiarize yourself with all the parts. Structure 1: Sample holder Display Screen...

  • Page 10: Chapter 4 Installation

    Smart-Q 800 Fluorometer Operation Manual Chapter 4 Installation Chapter 4 Installation Initial Check Each Smart-Q 800 has been factory-inspected prior to shipping. Please refer to the included packing list to ensure that all items are included. Contact Accuris or your local distributor if there are any signs of damage to the packaging upon receipt.
  • Page 11 Smart-Q 800 Fluorometer Operation Manual Chapter 4 Installation 8 strip PCR tube Fig 4.3.01 Instrument Operation Diagram...

  • Page 12: Chapter 5 Software Introduction

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Chapter 5 Software Introduction 5.1 Instrument Startup Power the instrument on. Upon start-up, the instrument will perform a self-check (Fig. 5.1.01). After the self-check, the Login Interface will be displayed (Fig. 5.1.02). Fig 5.1.01 Self-check Fig 5.1.02 Login Interface 5.2 Main Menu Interface...

  • Page 13: Dsdna Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Table 5-1 Main Menu Interface Operations Name Function Select to manage account settings or to switch between users. Select to view the working solution calculator Select to enter the dsDNA Interface Select to enter the RNA Interface Select to enter the Oligo Interface Select to enter the Protein Interface Select to enter the Fluorescence Interface...

  • Page 14: Dsdna Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Fig 5.3.01 dsDNA test type Fig 5.3.02 dsDNA test 5.3.1 dsDNA Interface Select the test type and click “OK” to enter next interface. The following takes dsDNA: High Sensitivity as an example. The specific functions are as follows: : Select an available standard curve.

  • Page 15: Calibration Curve Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction 5.3.2 Calibration Curve Interface Prior to sample measurements, a calibration curve must be created using the standards that are typically included with a quantification kit. Only 2 standards are required to form the calibration curve. button and select “Calibration”...

  • Page 16: Molarity Calculator

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction data from multiple samples within the same run. Click the name of the sample to modify the sample’s name. Fig 5.3.06 Testing interface Fig 5.3.07 Test Report Fig 5.3.08 Modify sample name : Delete selected test results.

  • Page 17: Normalization Calculator

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Select the conversion units: ng/μL, ng/mL, μg/μL, μg/mL, mg/mL. After selecting the conversion units, select the units: M, mM, μM, nM, pM. Input the molecular weight; the default value for dsDNA is set to 660g/mol. Input the DNA length, when “Auto-populate DNA length”...

  • Page 18: Fluorescence Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction concentration after dilution will be displayed (Fig. 5.3.12). The Initial Conc. interface shows the concentration of the original sample (Fig. 5.3.13). Click “Export” to export current data to an inserted USB drive. Fig 5.3.12 Secondary Dilution Fig 5.3.13 Initial Concentration of Sample 5.2 Fluorescence Interface...

  • Page 19: Working Solution Calculation Settings

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Test Types: Blue 470nm and Red 635nm are single excitation wavelength tests, whereas Blue & Red is a dual-wavelength test (Fig 5.4.03). The Blue 470nm interface is identical to the Red 635nm interface. Click to begin a sample measurement.

  • Page 20: Standard Curve Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction 5.4 Standard Curve Interface From the Main Menu Interface, click to enter the Standard Curve Interface (Fig. 5.6.01). Fig 5.6.01 Standard Curve Interface : Select the sample type (dsDNA, RNA, Oligo, Protein). : Create a new standard curve.
  • Page 21 Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Fig 5.6.02 New Curve Selection Box Fig 5.6.03 New Standard Curve Interface : Highlight the previous standard. : Highlight the next standard, or to add an additional standard. : View Parameter Settings (EX / EM wavelengths + concentration units). : Plot the obtained sample data points to a curve.

  • Page 22: Curve Management

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction 5.4.2 Curve Management From the Standard Curve Interface Click to enter Curve Management Interface (Fig. 5.6.04). Fig 5.6.04 Curve Management Interface Fig 5.6.05 Importing Curve(s) : Delete the selected curve. : Export the selected curve(s) to a USB drive (Fig 5.6.04). : Import curve(s) from a USB drive to the instrument (Fig.

  • Page 23: User Management Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Click on a report to view its saved data (Fig. 5.7.03). Click on the sample from the row to view the detailed sample report (Fig. 5.7.04). Fig 5.7.03 Saved Report Data Fig 5.7.04 Detailed Sample Report 5.6 User Management Interface From the Main Menu Interface, select the icon from the upper left corner of the...

  • Page 24: Settings Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction 5.7 Settings Interface Click in the main interface to enter the Settings Interface (Fig. 5.9.01). Fig 5.9.01 Settings Interface System Settings Upgrade Maintenance 5.7.1 System Settings Interface Fig 5.9.02 System Settings Click to enter system settings (Fig.

  • Page 25: Upgrade Interface

    Smart-Q Fluorometer Operation Manual Chapter 5 Software Introduction Date and Time: Set the system date and time. Screen Brightness: Adjust the screen brightness. Sleep Mode: Turn the automatic sleep mode on or off and adjust time required to initiate Sleep Mode. 5.7.2 Upgrade Interface From the Main Menu Interface, click to enter the Upgrade Interface (Fig 5.9.03).

  • Page 26: Chapter 6 Troubleshooting

    Chapter 6 Troubleshooting Issue Analysis Solutions Check the power supply; No display after Power not reaching Check whether the plug turning on power the instrument is loose. Check the power; Check whether the module is loose, if loose Light source not Light source failure;...

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